Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,556,628	C→T	Q74Q (CAG→CAA)	rtcA ←	RNA 3'‑terminal phosphate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,628	0	C	T	100.0%	192.9 / NA	54	Q74Q (CAG→CAA)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/26);  total (28/26)

GGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGCTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTCCACCAGATCGCTTTGTGCCGC  >  NC_000913/3556332‑3556918                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                   ggCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGNGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCAGCCGCGCACGGTGCAGGGCCGGAAGAGCAGACGTTGc                                                                                                                                                                                                                                                                                                 >  2:656937/1‑300 (MQ=255)           ccGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCt                                                                                                                                                                                                                                                                                       >  1:626327/1‑300 (MQ=255)            cGTTGCCACCACACCGCCTCCGTCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTc                                                                                                                                                                                                                                                                                      <  2:330968/300‑1 (MQ=255)                 ccaccaCACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCgcgc                                                                                                                                                                                                                                                                                 >  1:332408/1‑300 (MQ=255)                  caccacACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTTGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGTCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGcc                                                                                                                                                                                                                                                                                <  2:60103/300‑1 (MQ=255)                           tcgagatgtgtataatagacagTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCTGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGt                                                                                                                                                                                                                                                                       <  2:194821/279‑1 (MQ=255)                           gCCTCCGGCAGTGTAAAGACCTTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGTCGGATAAAATCCGCAGGCGGTGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCTGCGCCTTCCACGGt                                                                                                                                                                                                                                                                       <  2:196463/300‑1 (MQ=255)                           gCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCTGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGt                                                                                                                                                                                                                                                                       <  2:629671/300‑1 (MQ=255)                           gCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCNCCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGt                                                                                                                                                                                                                                                                       >  1:547554/1‑300 (MQ=255)                           gCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGt                                                                                                                                                                                                                                                                       <  2:4335/300‑1 (MQ=255)                           gCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGt                                                                                                                                                                                                                                                                       <  1:40559/300‑1 (MQ=255)                                                 tGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGtctgtctcttatacacatctccg                                                                                                                                                                                                                                                 >  1:195981/1‑278 (MQ=255)                                                   gCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTcgccgc                                                                                                                                                                                                                                               >  1:407838/1‑300 (MQ=255)                                                                             ttCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCa                                                                                                                                                                                                                     <  2:404739/300‑1 (MQ=255)                                                                                               gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCGACCGATAGCAAAGCGGTAATCGCCGCCGCGNACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTNAGATGCTGGCGCAACAGCCCCGGTTTcgccc                                                                                                                                                                                                   <  2:538633/300‑1 (MQ=255)                                                                                                cGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTcgcccg                                                                                                                                                                                                  >  2:153282/1‑300 (MQ=255)                                                                                                   cTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCgcccgccc                                                                                                                                                                                               <  2:675924/300‑1 (MQ=255)                                                                                                         cACCCGGCGGATAAAATCCGCAGGCGGCGCCTACGGGTTATCGGTGCCGCCGCTCACTTCAACACTCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACGATTTTCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCACATGGTGCCGGGCCTGAAGAGCATACGTTGCGACCCCAGCTCCACGCCTTCCACGGTTGCTCCGCAAATTTCCTTCGCCGCCTTTACCGCGGTCAGATGCTGGCACAACAGCCCCGGTTTCGCCCGCCCGGCACg                                                                                                                                                                                         <  2:31005/300‑1 (MQ=255)                                                                                                             cggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATg                                                                                                                                                                                     <  2:240366/300‑1 (MQ=255)                                                                                                                 ggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTgg                                                                                                                                                                                 >  1:313726/1‑300 (MQ=255)                                                                                                                   aTAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCTGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGTTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTg                                                                                                                                                                               <  2:385186/300‑1 (MQ=255)                                                                                                                            gCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAAt                                                                                                                                                                      <  1:272005/300‑1 (MQ=255)                                                                                                                               ggcggcGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGTAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACAGCGGTCAGATGCTGGCGCAACAGCCACGGTTTCGCCAGCCCGGCACGAATGCTGGTGATGGTAAAtggc                                                                                                                                                                   >  2:358529/1‑300 (MQ=255)                                                                                                                                          cGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGCACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCACCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTat                                                                                                                                                        >  2:419685/1‑300 (MQ=255)                                                                                                                                            ggTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTatca                                                                                                                                                      >  2:435269/1‑300 (MQ=255)                                                                                                                                             gTTATCGGTGCCGCCGCTCACTTCAACACGCNAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGNCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTatcat                                                                                                                                                     >  2:519490/1‑300 (MQ=255)                                                                                                                                             gTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTatcat                                                                                                                                                     <  1:569314/300‑1 (MQ=255)                                                                                                                                               tATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCg                                                                                                                                                   >  1:255639/1‑300 (MQ=255)                                                                                                                                                 tCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGAc                                                                                                                                                 <  2:106261/300‑1 (MQ=255)                                                                                                                                                            gcTCACTTCAACGCGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCAGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAgcgc                                                                                                                                      <  2:60142/300‑1 (MQ=255)                                                                                                                                                            gcTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAgcgc                                                                                                                                      <  1:594298/300‑1 (MQ=255)                                                                                                                                                                 cTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAgc                                                                                                                                 >  2:470781/1‑300 (MQ=255)                                                                                                                                                                   tCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGATGATGGTAAATGGCTGGCCGGTTATCATCAACAGGCTAAGCGCCGAgtgc                                                                                                                               >  1:570900/1‑297 (MQ=255)                                                                                                                                                                         cgcgAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCAGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCANATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCACGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATTGACAGGCTCAGCGCCGAGCGCAGGATc                                                                                                                         >  2:185017/1‑300 (MQ=255)                                                                                                                                                                               ggTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTAACGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCg                                                                                                                   >  1:145290/1‑300 (MQ=255)                                                                                                                                                                                gTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGc                                                                                                                  <  1:234354/300‑1 (MQ=255)                                                                                                                                                                                            aCCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAANAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCt                                                                                                      >  1:494504/1‑300 (MQ=255)                                                                                                                                                                                                      gggCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCNGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGACCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGTCGGTTATCATCGNCAGGCTCAGCGCCGAGCGCAGGATCTGCACGCCACCTTCGCCCTGTGCGCCATc                                                                                            >  2:684890/1‑300 (MQ=255)                                                                                                                                                                                                      gggCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATc                                                                                            >  1:45479/1‑300 (MQ=255)                                                                                                                                                                                                              ccGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAACTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCAGGCACGAATGCTGGTGATGGTAAACGGCTGGCAGGTTATCATCGACAGGCTCAGAGCCGAGCGCAGGATCTGCACGCCAACTTCGCCCTGTGCGCAATCCAGTGCaa                                                                                    >  2:66340/1‑300 (MQ=255)                                                                                                                                                                                                                gTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAatc                                                                                  >  2:469988/1‑300 (MQ=255)                                                                                                                                                                                                                                            cgcTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGc                                                      <  1:84749/300‑1 (MQ=255)                                                                                                                                                                                                                                                        cAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGTAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGTTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGTAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTGATCCTTTTACGCACACCACCTGAc                                          <  2:234378/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aaGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGc                                        <  1:198875/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    cgccgccgcGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAAGTTCCGTCGCCGCCTTTACCGCGTTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGTTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCGGCGCCGAGCGCAGGATCTGCCCGCCACCTTTGCCCTGTGCGCCGTCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGGATAGa                              <  1:656372/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      cctccgcGCACGGTGCCGGGCCGTAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGTTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATa                            <  2:105000/297‑1 (MQ=255)                                                                                                                                                                                                                                                                      ccgccgcGCACGGTGCCGGGCCGGAGGAGCAGACGTTGCGACCCCAGCTCCGCGNCTTCCACGGTTGCTCCGCAAATTTCCGGCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATa                            <  2:558682/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      ccgccgcGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATa                            <  1:592556/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           gcgcACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTc                       >  1:729235/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTCCACCAGa                >  1:99557/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATAGTAAATGGCTGGCCGGTTATCATCGAAAGGCTCAGCGCCGCGTGTAGGATCTGCCCGCCACATTCGCCCTATGCGCCATCCAGCGCAATCGTCCTTTTAATCATTTATCCTTTTCCGCCCCCCCCCTGAAGCAGGGTATAGATAGCTTCCACCAGa                >  2:719846/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gTGCAGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCAGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTAGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCCACTGACGAAGGGTATAGATAACTTACACCAGa                >  2:153458/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             gAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTCCACCAGATCGCTTTGTgc     >  1:649001/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                gagCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCAGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTCCACCAGATCGCTTTGTgccgc  >  1:195836/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                   GGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGCTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTCCACCAGATCGCTTTGTGCCGC  >  NC_000913/3556332‑3556918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑