Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,148,900	G→A	G335D (GGC→GAC)	gpr →	L‑glyceraldehyde 3‑phosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,148,900	0	G	A	100.0%	61.2 / NA	18	G335D (GGC→GAC)	gpr	L‑glyceraldehyde 3‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/12);  total (6/12)

CCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAACCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACTTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCC  >  NC_000913/3148611‑3149185                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                              ccGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAACCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATNAATGGCGCAAATGGCGTTAAGCTGGTNGCTGAAAGATGATCGCGTGACGTAGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACGAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAAt                                                                                                                                                                                                                                                                                     >  2:487201/1‑300 (MQ=255)                                        cGTGGTCTGACACCGAAAATGCTTACCGAAGCCAACCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAAc                                                                                                                                                                                                                                              <  1:318769/300‑1 (MQ=255)                                                                          aCCTCAGCAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTCTAGGCGCTGAATAATCTGACATTTAGNACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGNCGCGCGAACCGCGCCCGAATCAGATCAGTGa                                                                                                                                                                                                            <  2:513425/300‑1 (MQ=255)                                                                                               tgaatatactGGCACAACAGCGTGGACAACCAATGTCACAGATGGCGTGAAGCGGGTTGCTGAAAGAGGACCGCTTGACGTCGGTATTAATTGGTGCCACCCACGCGGACCAACTAGAGGAAAACGGACAGGTGCTGAATAATCTGACCTTTAGCACCAGGGAGCTGGCGCAGATTGGTCAGCACATCTCCGATGAGGAACTGAATCTGTGAAAGGCGTCTTCCGACAAATGACCTGTTAATAATGGGCACGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCa                                                                                                                                                                                       <  2:636646/291‑1 (MQ=255)                                                                                                   ggaagtGGCACAGCAGCGTGGACAATCAATGGCGCAAATTGCTTTACGCTGGTGGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTAGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGAt                                                                                                                                                                                   <  2:56012/295‑1 (MQ=255)                                                                                                        tGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGccc                                                                                                                                                                              <  1:604881/300‑1 (MQ=255)                                                                                                                     tGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAg                                                                                                                                                                 <  1:283196/300‑1 (MQ=255)                                                                                                                                 ggCGCAAATGGCGTTAAGCTGGTTGCTTAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACg                                                                                                                                                     <  2:396599/300‑1 (MQ=255)                                                                                                                                                           tGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTTTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATc                                                                                                                           <  1:62707/300‑1 (MQ=255)                                                                                                                                                                        cgTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGCGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGACGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGAAGTATCAGGTCGGTTTGTTATCCg                                                                                                              >  2:256158/1‑300 (MQ=255)                                                                                                                                                                                                             cAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTg                                                                         <  2:338098/300‑1 (MQ=255)                                                                                                                                                                                                              aaCTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGa                                                                        >  1:53108/1‑300 (MQ=255)                                                                                                                                                                                                              aaCTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTAAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGAGGCGTAGTAAGTGa                                                                        >  2:208749/1‑300 (MQ=255)                                                                                                                                                                                                                     ggagAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATNAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGgaag                                                                 <  1:497730/300‑1 (MQ=255)                                                                                                                                                                                                                                        aataatCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCTGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACgc                                              <  1:688315/300‑1 (MQ=255)                                                                                                                                                                                                                                                gACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACtt                                      <  2:339992/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    gCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCATATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACTTTGTTTTTCAGCGACGTTGCGTCCATTTTCCccagcc  >  1:229478/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACTTTGTTTTTCAGCGACGTTGCGTCCATTTTCCccagcc  >  1:716779/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                              CCGCAAGATTCACGGATGCATCGTGAAGGGAATAAAGTTCGTGGTCTGACACCGAAAATGCTTACCGAAGCCAACCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACTTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCC  >  NC_000913/3148611‑3149185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑