Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,570,269	G→A	P23P (CCC→CCT)	eutJ ←	putative ethanolamine utilization chaperonin EutJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,570,269	0	G	A	100.0%	202.4 / NA	57	P23P (CCC→CCT)	eutJ	putative ethanolamine utilization chaperonin EutJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/28);  total (29/28)

AGTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGC  >  NC_000913/2569985‑2570558                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                  aGTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGNCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGNAGATTCCGTCGCGGCAGGCGTCTGGTTacac                                                                                                                                                                                                                                                                                    <  2:524168/300‑1 (MQ=255) aGTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTacac                                                                                                                                                                                                                                                                                    <  1:3877/300‑1 (MQ=255)   tCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAg                                                                                                                                                                                                                                                                                  <  2:41481/300‑1 (MQ=255)                 gCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGttt                                                                                                                                                                                                                                                                    >  1:196100/1‑300 (MQ=255)                            gAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACTGTCGCGGTCGACAAACATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGTGTCTGGTTATACAGCGTTGCCGCCGTTTGCAGACGTggg                                                                                                                                                                                                                                                         >  2:408520/1‑300 (MQ=255)                             aaaTACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTgggg                                                                                                                                                                                                                                                        <  1:310753/300‑1 (MQ=255)                                       gTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGNTGCTCGAGCGTGTCGAGATTGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCATTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCNGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTg                                                                                                                                                                                                                                              <  2:693777/300‑1 (MQ=255)                                        tCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGt                                                                                                                                                                                                                                             >  2:401489/1‑300 (MQ=255)                                           gTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCAACAGCGGAGATTCCGTCGAGGCAGGCGTATGGTTACACAGCGTTGCCGCCTTTTGCAAACGTGGGGTGAGCCATTGTtcg                                                                                                                                                                                                                                          >  2:35708/1‑300 (MQ=255)                                                   aggaAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATGACTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTTGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTgcgc                                                                                                                                                                                                                                  <  2:635997/300‑1 (MQ=255)                                                               cgccgcATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTc                                                                                                                                                                                                                      <  1:582381/300‑1 (MQ=255)                                                                      tGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCTAAGAAATCCCAGAGGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAAc                                                                                                                                                                                                               <  2:77357/300‑1 (MQ=255)                                                                             aaCGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGNGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGa                                                                                                                                                                                                        >  1:534250/1‑300 (MQ=255)                                                                                             ctgctCGAGCGTGTCGAGATGGCGACGAACAATGATGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTacta                                                                                                                                                                                        >  2:666445/1‑297 (MQ=255)                                                                                              tgctCGAGCGTGTCGAGATGGCGACGAACAATGTTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATac                                                                                                                                                                                       <  2:95676/300‑1 (MQ=255)                                                                                              tgctCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGTCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATac                                                                                                                                                                                       <  2:44953/300‑1 (MQ=255)                                                                                               gctCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTACACAATGCGAAACGCATCGACTAATaca                                                                                                                                                                                      >  1:617755/1‑300 (MQ=255)                                                                                                        tgtagagaTGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGTATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATCCACAGCGACGc                                                                                                                                                                             >  2:276145/5‑300 (MQ=255)                                                                                                        tgtCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGaagc                                                                                                                                                                             >  1:556998/1‑297 (MQ=255)                                                                                                        tgtCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCACAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCGTCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGc                                                                                                                                                                             >  1:165473/1‑300 (MQ=255)                                                                                                                 ggCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTNACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACa                                                                                                                                                                    >  2:559084/1‑300 (MQ=255)                                                                                                                                             gaaATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCAc                                                                                                                                        <  1:137006/300‑1 (MQ=255)                                                                                                                                                aTCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTNTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCgg                                                                                                                                     >  2:644330/1‑300 (MQ=255)                                                                                                                                                aTCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCgg                                                                                                                                     <  1:735875/300‑1 (MQ=255)                                                                                                                                                                 cgcgAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCATGCGTCTGTTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCAGCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTc                                                                                                                    <  2:394572/300‑1 (MQ=255)                                                                                                                                                                      aCCACATCGGCCCATTCTAGGCATACCGCCACTTGCTGACCGTCGTGGTCGACAACCATCGACACCACATCGCAAGTGCCCAGATCCACCCCCATCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACACATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGTTTGGCGTGGTGATGTTCAtggt                                                                                                               <  2:285901/300‑1 (MQ=255)                                                                                                                                                                      aCCACATCGGCCCAGTCTAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCAtggt                                                                                                               <  2:360976/300‑1 (MQ=255)                                                                                                                                                                      aCCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCATGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTTAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGTTGATGGTCAtggt                                                                                                               <  2:273351/300‑1 (MQ=255)                                                                                                                                                                        cacaTCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCAtggtgg                                                                                                             <  1:61317/300‑1 (MQ=255)                                                                                                                                                                          caTCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTc                                                                                                           >  1:688413/1‑300 (MQ=255)                                                                                                                                                                                tgtataagagacagaCCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCt                                                                                                     <  1:713430/286‑1 (MQ=255)                                                                                                                                                                                    gTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTccc                                                                                                 >  1:242636/1‑300 (MQ=255)                                                                                                                                                                                    gTCGAGGCATACCGCAAATGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTccc                                                                                                 >  2:196493/1‑300 (MQ=255)                                                                                                                                                                                         ggCATACCGCCACTTGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCATGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCCGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGccc                                                                                            <  2:133643/300‑1 (MQ=255)                                                                                                                                                                                         ggCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGccc                                                                                            >  2:463344/1‑300 (MQ=255)                                                                                                                                                                                             tACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGcccagcc                                                                                        >  1:245110/1‑300 (MQ=255)                                                                                                                                                                                             tACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGAAGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGcccagcc                                                                                        >  2:476302/1‑300 (MQ=255)                                                                                                                                                                                              acctccactttccgACCTTCGCGGTCTACAACCAGCGAAGGCACATCGCAGGTGCCCAGATCTACCCCCAGCCACAGGGGAGATTCCGTCGCGGCAGGCGTCTAGTTACACAGCGTGGCCGACGTGTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACGCAGCGACGCAGACGGACAAACATACGCGCGCTGGGTACCCCTCCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGcccagccc                                                                                       <  2:258708/287‑1 (MQ=255)                                                                                                                                                                                              aCCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCNAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCNTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCtctgtctcttataca                                                                                       >  2:697379/1‑286 (MQ=255)                                                                                                                                                                                                   cACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCTGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCccagccccagcc                                                                                  <  1:463772/300‑1 (MQ=255)                                                                                                                                                                                                   cACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCccagccccagcc                                                                                  <  1:464945/300‑1 (MQ=255)                                                                                                                                                                                                      tGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCgg                                                                               >  1:424282/1‑300 (MQ=255)                                                                                                                                                                                                            aCCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGc                                                                         <  1:702118/300‑1 (MQ=255)                                                                                                                                                                                                                tCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTCAATCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGTAATGCACgg                                                                     >  2:37168/1‑300 (MQ=255)                                                                                                                                                                                                                       cGACAACCATCGACACCACATCACAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCACGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAGCAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTc                                                              <  2:251881/300‑1 (MQ=255)                                                                                                                                                                                                                            aCCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAAc                                                         >  2:471359/1‑300 (MQ=255)                                                                                                                                                                                                                                 cGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGaaaa                                                    >  1:394544/1‑300 (MQ=255)                                                                                                                                                                                                                                    caccacATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATNGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGc                                                 <  1:541169/300‑1 (MQ=255)                                                                                                                                                                                                                                        acaTCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGt                                             >  1:561774/1‑300 (MQ=255)                                                                                                                                                                                                                                         caTCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGGCGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTAATGGTGGTCCAGCCTTCACCGCCCAGCCACAGCCCGGCAATGCACGGTCCGTTCTTAACGAAGATGCTGGTa                                            >  2:572270/1‑300 (MQ=255)                                                                                                                                                                                                                                              cAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAAt                                       >  1:660509/1‑300 (MQ=255)                                                                                                                                                                                                                                                ggTGCCCAGATCCACCCCCAACCACAGCGGAGATTCCGTCGCGGCAGTCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAACCATTGTTCATCGTGCGCCAGCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTGACCCCTTCACCGTTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAg                                     <  2:229590/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gTGCCCAGATCCACCCCCATCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGNTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACCCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCNAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGc                                    <  2:697359/300‑1 (MQ=255)                                                                                                                                                                                                                                                   gCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCAt                                  >  1:401231/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGcc                             <  1:296592/300‑1 (MQ=255)                                                                                                                                                                                                                                                             aCCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTg                        >  1:91889/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   ccGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACNAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATNTTgcgc  >  2:611979/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                  AGTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGC  >  NC_000913/2569985‑2570558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑