| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,322,048 | (C)5→6 | coding (375/726 nt) | phnF ← | putative transcriptional regulator PhnF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,322,048 | 1 | . | C | 96.6% | 220.9 / ‑5.0 | 60 | E125D (GAG→GAT) | phnF | putative transcriptional regulator PhnF |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (25/32); minor base A (2/0); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CC‑TCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTG > NC_000913/4321749‑4322341 | tACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTccc‑cc < 1:742931/300‑1 (MQ=255) aCGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGa < 1:406505/300‑1 (MQ=255) ggtggtTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACg > 1:456257/1‑300 (MQ=255) gtggtTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGt > 1:718547/1‑300 (MQ=255) ggtTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGNACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGTGGCGCAGTGCAGTTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTACAGCGTCGGACAGGGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGAAACGACGCATGGTGNGCAGGTGGATGACGCTCTCCC‑CCATCGGTAATCCCCATTGCGTCAGCGCCGTgg > 2:700412/1‑300 (MQ=255) ggtTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGCAGCGTTGCAGCGTCGGCAAGAGGGTGAGGCCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCAAGTGCGTCAGCGACGTgg > 2:720556/1‑300 (MQ=255) ggtTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACCCGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCATCGGTAATCCCCAGTGCGTCAGCGACGTgg > 2:723634/1‑300 (MQ=255) ggtTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTgg > 1:729127/1‑300 (MQ=255) gggtAAGGGTGCGAACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCATGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCAGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCTGCGAAGTAGTGGTCGATTAACCAGAGCGCGACGCCGGTGCAACGACGCAGGGGGCGCAGGTGGATGAAATTCTCCC‑CCCTCGGTCATCCAAAGTGCGTCCGCGACGTgg > 2:723431/4‑300 (MQ=255) gTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCNGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGAcgc < 1:538522/300‑1 (MQ=255) cagcagCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATa > 1:102649/1‑300 (MQ=255) agcagcNGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGNGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATAc < 2:611873/300‑1 (MQ=255) agcagcGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCNCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATAc < 1:501925/300‑1 (MQ=255) actcaggATCTCAAGACGCTGGCACTCTTTGGCCTGGGTGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTTGCGCAGAAAATCGTGCAGCGCGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCGGGGTGCGCAGGGGGATGGCGTTCTCCAACC‑TCAATCATCCACAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGCAAGCAGttttt > 2:64289/6‑300 (MQ=255) gggATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGGCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCt < 1:127396/300‑1 (MQ=255) tCAAGACGCCTGCACTCTTTGGTCTGGGCGCGGCGGGCGCTGAGCCGCGTCTGGCTGCGGCGCAGCACAATTCCGGTTTGCTCGCGCAGAAAATCGGGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCAGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTggg < 2:10413/300‑1 (MQ=255) cAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGa < 1:322202/300‑1 (MQ=255) aCGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGc < 1:757889/300‑1 (MQ=255) aCGCTGGCACTCTGTGGCCTGGGCGCGGCGTGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGc < 2:202955/300‑1 (MQ=255) cTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGc > 2:311132/1‑300 (MQ=255) cTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGc > 1:248342/1‑300 (MQ=255) tgCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGGCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGccc < 1:165215/299‑1 (MQ=255) ggCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGccc < 1:165308/300‑1 (MQ=255) ctctTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGAt > 1:487784/1‑300 (MQ=255) ggCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCcagca > 1:566248/1‑300 (MQ=255) gCCTGGGCGCGGCAGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCTAAGCGTTGCAGCGTCGGCCAGAGGTTGAGGTCCGCGAAGTAGTGGTCGATGAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCTCGGATCcagcag < 1:281713/300‑1 (MQ=255) aTTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGgcg > 1:690664/1‑300 (MQ=255) tGCTCGCGCAGAAAATCGGGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGTTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGAGGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATcagcac < 2:159922/300‑1 (MQ=255) gCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATcagcacc < 2:578545/300‑1 (MQ=255) cacgcAGAAAGTCGTGCAGCGAGCCACTTTCGAAGCGTTGCAGCGTCAGCCAGAGGGTGAGGTCCGCGAATTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCTTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCACCTGGGCGTTGAGCGGGTAATCGAACGGGCGCaccagcaccagc < 2:12142/298‑1 (MQ=255) cGAGCCGCTGTCGAAGAGTTGCAGCGTCGGTCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATGAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGgc < 2:332307/300‑1 (MQ=255) cTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTAc < 1:686318/300‑1 (MQ=255) aGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTccac < 1:699403/300‑1 (MQ=255) agagGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCNGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCNCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCg < 2:581826/300‑1 (MQ=255) gagGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCTCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGa > 2:457613/1‑300 (MQ=255) gagGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGa > 2:462950/1‑300 (MQ=255) ccGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTNGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATggcgcggcgc < 1:518980/300‑1 (MQ=255) aaggagtGGTCGATGAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGgt < 2:481473/296‑1 (MQ=255) aaGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCNGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCNTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGgt < 2:687754/300‑1 (MQ=255) aaGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGgt < 1:197832/300‑1 (MQ=255) aaGTAGTGGGCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGgt < 1:83960/300‑1 (MQ=255) gtagtGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGgtgt > 1:155907/1‑300 (MQ=255) gtagtGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCACAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGTGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGgtgt > 2:82021/1‑300 (MQ=255) gtGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGc < 1:12122/300‑1 (MQ=255) tGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCg < 2:287319/300‑1 (MQ=255) ggTCGATTAAGAGGAGCGCGACGCCGTTGACACGACGTAGTGTGCACAGTTGGATGACGTTCTCCC‑CCCTCTGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTCTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCCGACGGCGCTGTACCCAGCCTTTTCCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGa < 2:233108/300‑1 (MQ=255) ggTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGa < 1:405039/300‑1 (MQ=255) gTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGAt > 1:270976/1‑300 (MQ=255) cGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCACAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTc > 2:38790/1‑300 (MQ=255) aTTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCAc > 2:7458/1‑300 (MQ=255) aTTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCAc > 1:105509/1‑300 (MQ=255) agagCGCGACGCCGTTTACACGGCGCAGGGTGCGCAGGTGGATGACGTCCTCCC‑CCCTCGGTAATCCCCAGTGCGGCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCTAGCAGATTCTGGCTAAAACGCGCCTGGGCGTGGAGCGGGTAATCGAGCGGGCGCATTAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTGTTTCCACCAGTTGGTCAATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAg < 2:372795/300‑1 (MQ=255) aacctcgACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGGGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAgc < 2:437325/295‑1 (MQ=255) gtcgACACTACGCAGGGTGCGCAGGTGGATTGCGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTTGGATGGCTGCCCTGATCCAGCAAATTCTGGCTAAAACGCGCCTGTGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGGCGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAGCGCGCTGCCAGtt < 2:225124/297‑1 (MQ=255) gacgCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTAACCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAACGCGCTGCCAGTTGCTGCTcg > 2:248889/1‑300 (MQ=255) aGGGTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCggg > 2:221245/1‑300 (MQ=255) gggTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCNGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGa > 1:497834/1‑300 (MQ=255) gggTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGa > 1:754342/1‑300 (MQ=255) gTGCGCAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAg > 1:419175/1‑300 (MQ=255) gcgcAGGTGGATGACGTTCTCCC‑CCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGAt < 1:320201/300‑1 (MQ=255) ggTGGATGACGTTCTCCC‑CCCTCGGTAATCCACAGTGCGTCAGCGACGTGGCAGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCACTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGAAGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAGCGCGCTGCCGGTTGCTGCTCGGCGGGAAGATAGTcg > 2:123492/1‑300 (MQ=255) tctcccc‑cc‑TCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAg < 1:587691/296‑1 (MQ=255) tcccc‑cc‑TCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAgtg < 1:208754/298‑1 (MQ=255) | TACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCC‑CC‑TCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTG > NC_000913/4321749‑4322341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |