Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,582	C→T	noncoding (35/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,582	0	C	T	96.0%	62.9 / NA	26	noncoding (35/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (13/11);  minor base G (0/1);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCT  >  NC_000913/696340‑696817                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTCTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                                                                                                                     <  1:102816/251‑1 (MQ=255)   cATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                                                                                                                   <  2:178217/251‑1 (MQ=255)                            gcgccttCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCGTGGTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGGTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaa                                                                                                                                                                                                          <  1:319512/246‑1 (MQ=255)                                          gaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATACGGTGCCTTACCGCTTGGCGATACCCCAACTGGGAGCACTTAAACGGTAAGCGTCTTGAATAAATTGGCTGGGGAAAGGGGATTCGAACCTGGGGATACAGGAATAAAAAACCGGTGCATTACCGCTTGGCAATACCCcaaaaacatgggtgtg                                                                                                                                                                                             >  2:305854/1‑237 (MQ=25)                                               aaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTg                                                                                                                                                                                       <  1:411570/251‑1 (MQ=255)                                                        aaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGGCTTGAATAAATTGGCTGGGGCACGAGGATCCGAACCTCGGAATGCCGGAATTAGAAGCGGGTGGCTTGCCGGTTGGCAATACCCCAATCAATTGGCTTTGacttatccgtacagc                                                                                                                                                                              >  1:380856/1‑237 (MQ=25)                                                             aaaaGCACGCCGACGCGAGCTTTTTTAATGAGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCAGAATACGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGa                                                                                                                                                                         <  2:399120/251‑1 (MQ=38)                                                              aaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGAACTTAAAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGATTTGAATTTGCCGAACATATTCGAt                                                                                                                                                                        >  1:223894/1‑251 (MQ=255)                                                                    cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACGAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTAAATTTGCCGAACATATTCGATACATTc                                                                                                                                                                  <  1:129621/251‑1 (MQ=255)                                                                         aagcgatctagtttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGATAAGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATGAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAAt                                                                                                                                                             <  2:248560/240‑1 (MQ=255)                                                                                      taaggtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGAGATACCCCAACTGGGTGCACTTACAAGGTAAGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTacg                                                                                                                                                 <  2:400746/246‑1 (MQ=255)                                                                                                                                    aGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTTCCGTACATATTCGCTACCTTCAGAAATTGGGGCCTACTACGGTATTAGTGCCTGTGACCCCATCATTCTGTATGATGTGCACTa                                                                                                   >  2:448442/1‑250 (MQ=25)                                                                                                                                                            cGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGAtt                                                                          >  1:288414/1‑251 (MQ=255)                                                                                                                                                             gATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTGGCCGAACATATTAGATCCCTGCGGAATTTGGTGGTGAAGACGGGATTAGAACCTGTGACCCCCACATTATTAGTGATGTGCTCTAAACAAATGAACTACCTAGCCAGATTg                                                                         >  2:337971/1‑251 (MQ=25)                                                                                                                                                                     aaCTGGGTGCACTTACATGGTAAGCGTCTTGAATAAATTGGCTGGGGTATGAGGATTCGAACCTAGGAATGCCGGAATTAGAATCCGGCGCCTTAACCCTTGCCGAAACCACAACGAATTGGTTTTGAATTTGCCTACCATACTCGATACATTCAGAATTTGCTGGCTACGTCGGGATTCGAACCTGTGACCCCATCATTTTGAGGGATGTGCTCTAATCAACTGACATACGAAGCCAGAATGGTTCTTCg                                                                 >  2:341795/1‑251 (MQ=255)                                                                                                                                                                     aaCTGGGTGCACTTACAAGGTAAGCGTCTTGAATACATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACCAATTGGGTTTTAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACTACGGGATTCGAACCTGGTACCCCATCATTATGCGTGCTGCGCTCTAACCAACTGAGCTACGCAGCCatcttcttgagtcc                                                                 >  2:334470/1‑238 (MQ=25)                                                                                                                                                                                              gTCTTGAATCAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAATAGAATCCGGTGCCTTACCGCTTGGCGCTACCCCACCAGCTTGGTTTTGAATTGGCCGAACATATTCGCTACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCAATATGAGCGATGGGCTCTAACCCACAGAGCGACGTAGCCAAATTGTTTATTCGATGGGTGGGCTACCTGAATTCGaa                                         >  2:36798/1‑250 (MQ=25)                                                                                                                                                                                              gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAAc                                        <  1:160406/251‑1 (MQ=255)                                                                                                                                                                                                 ttGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAACTTGGCTTTGAATTTGCCACACATATTCGATCCATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGAACCCATCATTATGAGTAATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGaagc                                       >  1:46789/1‑247 (MQ=38)                                                                                                                                                                                                           tGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTGTTGAAGTTGCCGAACATGTTCGATACATTCAGAAGTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATTCCgg                           >  2:409779/1‑251 (MQ=255)                                                                                                                                                                                                                ggggTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGGCCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTACGATGGCTGGGGTACCTGGATTCGAGCCAGGGAATGCCGGTATCa                      <  1:169691/251‑1 (MQ=255)                                                                                                                                                                                                                        aGGATTCGAACCTCGGCATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCCGAATTTGATGGCTACGCCGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACAAAGCTACGTAGCCAGCTTTTTTCTTCGATGGCTGGGTTACCATGATTGGAACCAGGGTATgtatgtaacaataaccgg              >  1:298296/1‑234 (MQ=25)                                                                                                                                                                                                                         ggATTCGAACCGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATGCGACCCAGGGAATGCCGGTATCAAAAACCGGt             <  1:121962/251‑1 (MQ=255)                                                                                                                                                                                                                              cGAACCGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCATCAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGAATCGAACCTGGGAGCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTCCGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCt         <  1:341795/250‑1 (MQ=39)                                                                                                                                                                                                                                aaCCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGCGACCGCCTCATTATGAGTTATGTGCTCTAACCAAATGAGCTACGTCGCAGGATCGTTTCTTCGATGGCTGGGTTACCTGGATTCTAACCAGGGAATGCCGGTATCCAAACCCggagtctaac      >  2:393402/1‑243 (MQ=255)                                                                                                                                                                                                                                    tCGGAATGCCGGAATTAGAATCCGGTGCCGTACCGCTTTGCGATACCCCAACACATTGGTGTTGAATTTGCCGATCATATTCGATACATTCAGAATGTGGAGGCTACGACGGGATTCGAACCTGTGACCCCACCATTATGAGAGATGGGCTATAACCAACTGAGCTACGTCGCCAGATTGATTCTTCGATGGCTGGGTTCCCTGGATTCGAACCAAGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCt  >  1:196056/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            GACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCT  >  NC_000913/696340‑696817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑