Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,816,088	G→A	Q118* (CAG→TAG)	gshA ←	glutamate‑‑cysteine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,816,088	0	G	A	100.0%	132.1 / NA	39	Q118* (CAG→TAG)	gshA	glutamate‑‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/22);  total (17/22)

GATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCGCAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTGTGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCGGTAGTAATCCATTTGTGCGTCAGTGCGGAACCTAATGCTTCAGGATGACCTGTTGTT  >  NC_000913/2815851‑2816320                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                         aaTAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCGCAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGGGGTAGAAGTGCCGTACTATGCCAGTTCGATg                                                                                                                                                                                                                             <  1:440886/250‑1 (MQ=255)                                   gCGGATAACGCGGAAATAGCCCGCAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAACGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACtt                                                                                                                                                                                            >  2:451724/1‑250 (MQ=255)                                     ggATAACGCGGAAATAGCCCGCAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAAc                                                                                                                                                                                         <  1:299208/251‑1 (MQ=255)                                       aTAACGCGGAAATAGCCCGCAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGAGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACgg                                                                                                                                                                                       >  1:26201/1‑251 (MQ=255)                                                  aTAGCCCGCAGAAATTTTCTCTTTGGCATCAGCGCCCAAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGGACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGTCCGTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCt                                                                                                                                                                            <  2:359420/251‑1 (MQ=255)                                                                       tttGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCg                                                                                                                                                       >  2:522213/1‑251 (MQ=255)                                                                       tttGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCg                                                                                                                                                       >  2:60001/1‑251 (MQ=255)                                                                             aTCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAAc                                                                                                                                                 >  2:193921/1‑251 (MQ=255)                                                                                 gcgcCCGAGATATCACCGCACTGCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATg                                                                                                                                             <  2:496226/251‑1 (MQ=255)                                                                                          atatCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGATTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATcgcg                                                                                                                                    <  2:261203/251‑1 (MQ=255)                                                                                            atCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCgcgc                                                                                                                                   >  2:155006/1‑250 (MQ=255)                                                                                                 cGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAg                                                                                                                             <  1:522213/251‑1 (MQ=255)                                                                                                    aCTTCGCTTGCCAGAATGCCATTGGCAAAGAGCAATTGAAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTc                                                                                                                          >  1:155074/1‑251 (MQ=255)                                                                                                        cGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGc                                                                                                                       >  1:165697/1‑250 (MQ=255)                                                                                                         gCTTGCCAGAATGACATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAAGGGTTTGCATCAGCGCGCCGTAGCGATTGTTCAGCCCTTCAAGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCGTCTGCGATGTAGCATGGCATACTGAGCGGCGACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCat                                                                                                                     <  2:276319/251‑1 (MQ=255)                                                                                                              ccAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGtt                                                                                                                <  1:491480/251‑1 (MQ=255)                                                                                                                aGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCa                                                                                                              <  1:155006/251‑1 (MQ=255)                                                                                                                 gAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAACTGGCTTGCATCAGCGCGCCGTAGCGATTTTTAAGCGCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGCCCTTCTGCGATGTAGCATGGCATACTTACCGGCCCCATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCCGATCGCGCATAAGGGTCAGCATATGTTCa                                                                                                              >  2:505612/1‑250 (MQ=255)                                                                                                                  aaTGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAat                                                                                                            >  1:351214/1‑251 (MQ=255)                                                                                                                      ccATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCa                                                                                                        <  1:146499/251‑1 (MQ=255)                                                                                                                                agagAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTg                                                                                              <  1:162490/251‑1 (MQ=255)                                                                                                                                   gaAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGgtg                                                                                           >  1:488828/1‑251 (MQ=255)                                                                                                                                    aaaTTGTAGGGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCTTAAAGGTCAGCATATGTTCAATATCACCATCCACTGgtgt                                                                                          <  1:33512/251‑1 (MQ=255)                                                                                                                                                  gCCGGAATTGGTTTGCATCAGCGCGCCGTAGCGATTTTTCCGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGATCGATGCCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGc                                                                             >  2:62287/1‑250 (MQ=255)                                                                                                                                                   acGGAAATGGGTTGCATCAGCACGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAATTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCAGCGCCCATATTGCGCGCCGTATATCGATGCATATCGCGCATAAAGGTCAGCAGATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCaa                                                                           <  2:275245/250‑1 (MQ=255)                                                                                                                                                        aaTGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGAAAGCATATGTTCAATATCACAATCCACTGGTGTAATGAATTCCAGCAATGCtt                                                                      >  1:237161/1‑251 (MQ=255)                                                                                                                                                                       cgcgcCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCGgt                                                         >  2:460944/1‑249 (MQ=255)                                                                                                                                                                           ccGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCGGTAGTAAt                                                   <  1:60001/251‑1 (MQ=255)                                                                                                                                                                             gTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGTGTCCGGTGTTAGAAGTGCCGTACTATGCAAGTTCGATGTCCTCACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGGAATGAATTCCAGCAATGCTTCCGCACAATCGGAAGTAATcc                                                 >  2:460375/1‑251 (MQ=255)                                                                                                                                                                                      tttCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCGGTAGTAATCCATTTGTGc                                         <  2:351214/250‑1 (MQ=255)                                                                                                                                                                                               ttCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCGGTAGTAATCCATTTGTGCGTCAGTGCgg                               >  1:304846/1‑251 (MQ=255)                                                                                                                                                                                                   cGATACAGCGTTTTAAAGCTTCCGGTGTTATAAGTGCCGTACTATGCCAGTTCGATGTCCTGGCCTTCTGCAATGTAGCATGGCATACGTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTTGTGTAATGAAGTCCAGCAATGCTTCCGCAAAATCTATATTAATCCCTTTGTGCGTCAGTGCGGatcc                           <  1:282473/251‑4 (MQ=255)                                                                                                                                                                                                     aTACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTAGATGTCCTGTCCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTTATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATTCTTCCGCAAAATCGGTAGTAATCCATTTGTGCGTCAGGGCGGAACCTa                         <  2:344206/251‑1 (MQ=255)                                                                                                                                                                                                         aGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCGGTAGTAATCCATTTGTGCGTCAGTGCGGAACCTAATGc                     >  1:428342/1‑251 (MQ=255)                                                                                                                                                                                                          gCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCGGTAGTAATCCATTTGTGCGTCAGTGCGGAACCTAATGCt                    <  1:296839/251‑1 (MQ=255)                                                                                                                                                                                                                        ccGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCGGTAGTAATCCATTTGTGCGTCAGTGCGGAACCTAATGCTTCAGGATGACCtg       <  2:405308/250‑1 (MQ=255)                                                                                                                                                                                                                        ccGGTGTTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCGGTAGTAATCCATTTGTGCGTCAGTGCGGAACCTAATGCTTCAGGATGACCtg       <  1:368901/250‑1 (MQ=255)                                                                                                                                                                                                                             gtTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCTGTAGTAATCCATTTGTGCGTCAGTGCGGAACCTAATGCTTCAGGATGACCTgttgtt  <  2:488828/250‑1 (MQ=255)                                                                                                                                                                                                                             gtTAGAAGTGCCGTACTATGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCGGTAGTAATCCATTTGTGCGTCAGTGCGGAACCTAATGCTTCAGGATGACCTgttgtt  <  1:395456/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                         GATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCGCAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGCGTTTTAAAGCGTCCGGTGTTAGAAGTGCCGTACTGTGCCAGTTCGATGTCCTGACCTTCTGCGATGTAGCATGGCATACTTAACGGCCACATCCGCTCATCGCCCATATTGCGCGCCGTATAACGATGCAGATCGCGCATAAAGGTCAGCATATGTTCAATATCACCATCCACTGGTGTAATGAATTCCAGCAATGCTTCCGCAAAATCGGTAGTAATCCATTTGTGCGTCAGTGCGGAACCTAATGCTTCAGGATGACCTGTTGTT  >  NC_000913/2815851‑2816320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑