Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,705,108	G→A	G54R (GGG→AGG)	cnu →	H‑NS‑ and StpA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,705,108	0	G	A	100.0%	77.5 / NA	22	G54R (GGG→AGG)	cnu	H‑NS‑ and StpA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/10);  total (12/10)

ACAAGCGTTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCGGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGCTCTGTTGAGTACGACGCTGGCGTTGTTGCTGTATAC  >  NC_000913/1704869‑1705353                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      acaAGCGTTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCgtttgttt                                                                                                                                                                                                                                            <  1:121078/251‑1 (MQ=255)                           tATCATAACTAATAGTACCTACCCCTTTGGGATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGACTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGCACTGATCAATATGTATCGTGATGCCGATCACCGTCGCGCAGAGATGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCCGGTACCGAAGTCCGt                                                                                                                                                                                                                  >  2:300819/1‑251 (MQ=255)                                tcaCTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGc                                                                                                                                                                                                             >  2:456443/1‑251 (MQ=255)                                     aataatACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTa                                                                                                                                                                                                         <  2:249905/250‑1 (MQ=255)                                           acctaccCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCaa                                                                                                                                                                                                  <  2:59277/251‑1 (MQ=255)                                                 cccTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATaa                                                                                                                                                                                               >  2:378055/1‑248 (MQ=255)                                                   cTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATaaagaa                                                                                                                                                                                           >  2:193639/1‑250 (MQ=255)                                                        gTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGc                                                                                                                                                                                     <  2:244186/251‑1 (MQ=255)                                                             aattaTTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCg                                                                                                                                                                                >  2:82821/1‑251 (MQ=255)                                                                   tttATGGACATGTTATGACTGTTCAGGACTACTTATTAAAAGTACGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACTATCTTAATTACACCTCGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTTCAATAAAGAAAGTAGCGATCGGTtat                                                                                                                                                                           <  2:179428/250‑1 (MQ=255)                                                                               ttATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTtata                                                                                                                                                               <  1:378055/250‑1 (MQ=255)                                                                                               ctactTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATaaaa                                                                                                                                                                       >  2:172808/1‑226 (MQ=255)                                                                                               ctactTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATaaaa                                                                                                                                                                       <  1:172808/226‑1 (MQ=255)                                                                                                                              cTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTgg                                                                                                                 >  1:105032/1‑249 (MQ=255)                                                                                                                                     gTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTAt                                                                                                        >  1:359570/1‑251 (MQ=255)                                                                                                                                                   tACGACCCTCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCAccacaacgc                                                                                          >  2:47634/1‑251 (MQ=255)                                                                                                                                                                      cccTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTg                                                                       >  2:217062/1‑251 (MQ=255)                                                                                                                                                                            cggacgAACAGGAACTGATCAATATGTATCCTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGTCCAGGGACCGAAGTCCGTCTGTCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGTTTTGCCTGTTTGCGTCGCTGTTCTGGAGACCCTATAACCACCACAACGCCACAACGTATTGGAGGCTGGTTGc                                                                  <  2:289307/250‑1 (MQ=255)                                                                                                                                                                                        aaCTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGTCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCTTCTGGCACTAt                                                                                                                                                                                                        <  2:74415/104‑1 (MQ=255)                                                                                                                                                                                                       aTCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGc                                      <  2:359570/251‑1 (MQ=255)                                                                                                                                                                                                                  gATCACCGTCGTGCAGAGCTGGTTTCTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGCTCTGTTGAGt                            >  2:191368/1‑250 (MQ=255)                                                                                                                                                                                                                                           cTGGCAGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGCTCTGTTGAGTACGACGCTGGCGTTGTTGCTGTATAc  >  1:332566/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      ACAAGCGTTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACCATCTTAATTACACCCTGACGGACGATCAGGAACTGATCAATATGTATCGTGCTGCCGATCACCGTCGCGCAGAGCTGGTTTCTGGCGGGCGTTTGTTTGACCTCGGCCAGGTACCGAAGTCCGTCTGGCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGCTCTGTTGAGTACGACGCTGGCGTTGTTGCTGTATAC  >  NC_000913/1704869‑1705353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑