Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	73.7 / NA	23	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/10);  total (13/10)

GTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCAT  >  NC_000913/1652092‑1652573                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   gtgtTTCTGTATATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTa                                                                                                                                                                                                                                          <  1:425905/250‑1 (MQ=255)            tataCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAACGGGTCATTCATCTTTTTAAACCAGCAAACGGCGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGCATATCATTGATGTTCAACTCAGAGAGTATGGcagaac                                                                                                                                                                                                                                 >  2:298629/1‑248 (MQ=255)                      ttCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAAAGGGGCATTCATCTTATTAAGACAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGCATATCATTTATGTTCACCTCAGAGAGTATGGCAGAACAGaaaaaaaaca                                                                                                                                                                                                                     >  2:179381/1‑245 (MQ=255)                           ccTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCAc                                                                                                                                                                                                               <  1:197027/251‑1 (MQ=255)                               gTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCttt                                                                                                                                                                                                           <  2:92957/251‑1 (MQ=255)                               gTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCCGGTCATTCATCTTATTAAGCCAGCCATCGGCGCTCTCCGGAGTCAGATGAAATTAACGAGAATGAGCAAAGAGCATATCATTGATATTCAACTCAGAGAGTATTGCAGAACAAAAAAACAAAAAAGCACCttt                                                                                                                                                                                                           >  1:324008/1‑251 (MQ=255)                                               tGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGaa                                                                                                                                                                                            <  2:362703/250‑1 (MQ=255)                                               tGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGa                                                                                                                                                                                             <  2:412227/249‑1 (MQ=255)                                                              aGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATAGGGGCGTTCATTTTATTAAGCCAGCAAACGACGCTCTCCGGAGTCAGAAGAAATTAACGAAACTGAGCAAAAAGCATATCAGTGATGTTCACCTCAGAGAGGATGGCAGAACAGAAAAAAAAAACTGCAACTTTTTTTTTCAAACTGAAggggacacgagagaa                                                                                                                                                                             >  1:506139/1‑236 (MQ=255)                                                                 tCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGGCAGATGACATTAAAGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTTAACCTGAAGTGTCAGCGAGAGGaaaa                                                                                                                                                                          >  1:378354/1‑250 (MQ=255)                                                                                 atgatgATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTGCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCAttt                                                                                                                                                         <  1:516040/251‑1 (MQ=255)                                                                                       atCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGt                                                                                                                                                   <  2:401973/251‑1 (MQ=255)                                                                                          aGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAAAAGGGTCATTCATCTTATTAAGCCATCAATCGACGCTCTCCGGGGGCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTTACCTCAGAGAGAATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTTAAGGGTCAACGAGAGTAAAAAATTATGGTGAACATTTTACCGttt                                                                                                                                                 >  2:267005/1‑249 (MQ=255)                                                                                                                           ccAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAtt                                                                                                                                                                      <  1:177299/196‑1 (MQ=255)                                                                                                                           ccAAAAACACAGGCGGGAACCAAACGGGTCATTCATCTTATTAAGCCAGCAATCGACGCCCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAAAAAAAAAAAAATGCACCTTTGTTTTTTAACCTGAAGTGTCAGCGCGAGTaaaaaaat                                                                                                                                                                      >  2:177299/1‑194 (MQ=255)                                                                                                                                        taggAACCAATCGGGTCATTCATATTATTAAGCCAGCAATCGACGCTATCCGGAGTCAGATGACATTAACGAAACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCAACTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGAGCCGGACTc                                                                                                  >  2:115033/3‑251 (MQ=255)                                                                                                                                                                              aTCGACACTCTCCGGAGTCAGATGAAATTAACGAGACGGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAAAGAAAAAAAAAAATGCACCTTTGTTTTTCAAACTGAAGTGTCAGCGAGAGGAAAAAATTATGGGGACCATATTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGACAAAACGTGCCGGAGTCGGACATCGAAAATCATATCAGACGAGACATACTTATg                                                             >  2:94490/1‑250 (MQ=255)                                                                                                                                                                                                  gATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACACCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTg                                        >  1:360820/1‑251 (MQ=255)                                                                                                                                                                                                                    gAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGGCCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCAttt                       <  2:508119/250‑1 (MQ=255)                                                                                                                                                                                                                            agCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAAc              <  2:324008/251‑1 (MQ=255)                                                                                                                                                                                                                               atatCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAAcc             >  2:92790/1‑249 (MQ=255)                                                                                                                                                                                                                               atatCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAAcc             >  1:211666/1‑249 (MQ=255)                                                                                                                                                                                                                                        aTGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCAt  >  1:214607/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   GTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCAT  >  NC_000913/1652092‑1652573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑