Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,859	T→C	Y110C (TAT→TGT)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,859	0	T	C	100.0%	77.7 / NA	27	Y110C (TAT→TGT)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/12);  total (15/12)

CCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGAACCGGCCT‑CCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATC  >  NC_000913/1358634‑1359101                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       ccTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGACGGAGATTTGCACCAGTACAAACAGGGGATTGAAGTTAGCGACCATGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACGCCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCACCAAGACGTTAATCATCTGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGAc                                                                                                                                                                                                                               <  1:33095/251‑1 (MQ=255)    ttATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGAcc                                                                                                                                                                                                                              <  1:519791/249‑1 (MQ=255)      aTGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATa                                                                                                                                                                                                                           <  1:148201/250‑1 (MQ=255)            tCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGCCGAAAGGTT‑CCACCCACACCCACGGCGGCACCTCCGGGGAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGGGATGACCACCCGACCATAAAACCg                                                                                                                                                                                                                     >  1:278888/1‑250 (MQ=255)                           gAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCg                                                                                                                                                                                                    >  1:461084/1‑251 (MQ=255)                                           atgatgGAGATTTGAACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTGAAAGAGGGACGGA‑GGGTACCACACACACCCACGGCGGGACTTCCGGGAAGGGGGGGGGGAGAGAGGTTTTCGCCAAAAAGACGTTAATCATCGGCAAAAAGAGACAAACCAGCAGGGAAGAACAGCCGACCATAAAGACGACGTGCGGG‑GTAATCG‑ACTTTTGCGCGtcggg                                                                                                                                                                                   >  2:94613/1‑247 (MQ=255)                                                      ttGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGaa                                                                                                                                                                        <  2:86804/250‑1 (MQ=255)                                                        gCNCCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGAAcc                                                                                                                                                                      <  2:482533/250‑1 (MQ=255)                                                            cAGTACAACCAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGAACCGGCCt                                                                                                                                                                 <  2:190140/251‑1 (MQ=255)                                                              gTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGGTACCACCCCCACCCACGGCGGGACTTTCGGGAAGAGGGCGGGGAGAACGATTTTCGCCAACAAGACGTTAAACATCGGCAAAAAGAGACAATCCAGCAGCGACGACCAGCCGACCATAAATCCGACGTGCGGGGTTAA‑CG‑ACTTTTGCGGGCAGGGATAGG‑CCGAACCGGCCT‑cc                                                                                                                                                              >  1:387157/1‑251 (MQ=255)                                                                aCAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGCGAGAAAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGAGTACCAGCCGACCCTAAATCCGACGTGCGGG‑CTAATCG‑ACTTTTGCGCGTCGGTATAGG‑GCGAACCGGCAT‑CCgg                                                                                                                                                            >  1:18025/1‑251 (MQ=255)                                                                             tGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAAGCGCCGAC‑GGTTACCACCCACACCCCCGGCGGCACTTCCGGGAAGAGGGCGGAGAGATCGATTTTTGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATTCAGCAGCGATGACCCACCGACCATAAATCCGACTCGCCGG‑TTTACCG‑ACTTTTGTGCGTAGGTATAAG‑CCGGACCGGCCTTCCGGAA‑CTGGCGAAcc                                                                                                                                               >  1:231412/1‑251 (MQ=255)                                                                                      ggaccatgttaacactctcaaGATTCGCGGCGGTTAAAAGGGCGACGAA‑GGCTACCACCCACACCCACGTCGCCACATACGCGAATAGGGCGGAGAGATAGATTTTCGCCAACAAGTCGTTAATCAACGGCAAAAAGAGACAATTCCCCAGCGATGACCAGGCGACCACAAACTCGACGAGCGTG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCCAACCGGCCT‑CCGGAAACTGGCGAACCAGTTTGcct                                                                                                                                      <  2:326466/231‑2 (MQ=255)                                                                                              ttGACGCTCTTCAGATTCGCGGCGGTTAAAATAGGGACGAA‑GGTTACCACCCACCCCCCCGGCGGCACTTCCGGGAAGACGGCGGGGTGATAGATTTTCGCCCACAAGACGTTAAACATCGGCAAAAAGAGACAATCCCGCATCGATGACCAGCCGACCATAAAACCTGCCAGCGGG‑TTAATCG‑ACCTTTTCGCGTAGGGATAGAACCGA‑CCGGCCT‑CCGGAAACTAGAGAACCCGTTTgtccgagctagt                                                                                                                              >  2:268071/1‑240 (MQ=255)                                                                                                  cGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGTGCGTAGGTATAAG‑CCGAACCGGCCT‑CCGGGAACTGGCGAACCAGTTTGCCGTAGCTGATAGCg                                                                                                                          >  2:12141/1‑251 (MQ=255)                                                                                                   gCTCTTCAGCTTCGCGGCGGGAAAAATAGCGGCGAA‑GGGTTCAACCCACACCCACGGCGGCACCTCCGGGGAGAGGGCGGAGAGATAGATTTTCGCCAACACGACGTTTATAATCGGCAAAAAGAGACAATCCAGCAGCGAAGACCCGCCGACCCTAAATCCGACGTGCGGG‑TTAATTGCACTTTT‑CGCGTAGGGATAAG‑CTCAAACGGCCT‑CCGGAAACTGGCCAACCatgttaccgttgccgaaagtgg                                                                                                                         >  2:325052/1‑230 (MQ=255)                                                                                                                  ggcggTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGAACAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGAACCGGCCT‑CCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGcaccaccg                                                                                                          >  2:236030/1‑246 (MQ=255)                                                                                                                    cggTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGAACCGGCCT‑CCGGAAACTGGCg                                                                                                                                                   <  1:64067/208‑1 (MQ=255)                                                                                                                    cggTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGAACCGGCCT‑CCGGAAACTGGCg                                                                                                                                                   >  2:64067/1‑208 (MQ=255)                                                                                                                                          ttACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGAACCGGCCT‑CCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGCAAACAGCACACCCGCCAGCGGCATCAAATAGGACGcc                                                                                   >  2:444590/1‑251 (MQ=255)                                                                                                                                              cccgcacccccggggcggctttttcGGGAAGAGGGCGGAAAAATGGATTTTCTCCAAAAAGACTTTAATCATCCGAAAAAAGAGACAATCCAGGATCTAGGACCAGCGGACAATAAACCATACGTGGGGG‑TGAATCG‑ACTTTTGCGCGGAGGTATGGG‑CCGACCCGGCCC‑CCGGAACCGGGCGAACCGGGTTGCCGTGGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGaa                                                                               <  2:278888/227‑1 (MQ=255)                                                                                                                                                           ggcggcACTTCAGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACCAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGAGCGTAGGAATAGG‑CCGAACCGGCCT‑CCGGAACCTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAAAAGCACACCCGCCAGCGCCACCACATAGGACGCCGGAACGTGACCGTCGc                                                                   >  2:182603/1‑250 (MQ=255)                                                                                                                                                                                      agatagatTTTCCCCAACAAGACGTAAATCATCGGCAAAAAGAGACAACCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TAAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGAACCGGCCT‑CCGGAAACGGGCGAACCAGTTTGCCGTAGCTGAGAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATc                                       <  2:461084/251‑1 (MQ=255)                                                                                                                                                                                                  gCCAACAAGACGTAAATCATCGCCAAAAAGAAACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGAACCGGCCT‑CCGGAAACTGGCGAACCAGTTGGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCAt                           <  1:70914/251‑1 (MQ=255)                                                                                                                                                                                                         aGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAAACG‑ACTTTTGCGCGTAGGTATAGG‑CCGAACCGGCCT‑ACGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGAGTg                    >  1:325022/1‑251 (MQ=255)                                                                                                                                                                                                                 accaccGGCAAAAAGAGCCACTCCAGCAGCAATGCCCAGCCCACCATAAACCCGCCGTGCGGT‑TTAACCG‑CCTTTTGCCCTTAGTTATAGT‑CCGACCCGCCCT‑CCGGAACCTGGCAACCCATTTTGCCGTAGCTGATAGCGGTAACCACCACACCCGCCAGCGCCATCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGc            <  2:282700/246‑1 (MQ=255)                                                                                                                                                                                                                            aaaGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGAACCGGCCT‑CCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAACTACGGTCATCGGCGTGAGATAGGCCAGACCcatc  >  1:217668/1‑250 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       CCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGG‑TTAATCG‑ACTTTTGCGCGTAGGTATAGG‑CCGAACCGGCCT‑CCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATC  >  NC_000913/1358634‑1359101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑