Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,942,765	Δ8 bp	noncoding (70‑77/206 nt)	gcvB →	small regulatory RNA GcvB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2942765	2942772	8	30 [0]	[1] 31	gcvB	small regulatory RNA GcvB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2942764	0 (0.000)	30 (0.640)	29/476	0.6	100%	noncoding (69/206 nt)	gcvB	small regulatory RNA GcvB
	?	NC_000913	2942773 =	0 (0.000)					noncoding (78/206 nt)	gcvB	small regulatory RNA GcvB

TAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCGGTATCGTTATCAGTCTG  >  NC_000913/2942544‑2943022                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                          aaggcatttagcggtggtaatcgtttagacatggctattaaactttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCt                                                                                                                                                                                                                                      >  2:151431‑M2/230‑251 (MQ=255)            cggtggtaatcgtttagacatggctattaaactttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGAc                                                                                                                                                                                                                           <  1:207963‑M2/33‑1 (MQ=255)                   aatcgtttagacatggctattaaactttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGt                                                                                                                                                                                                                     >  1:390027‑M2/212‑250 (MQ=255)                                     attaaactttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCttttt                                                                                                                                                                                                    <  2:364087‑M2/56‑1 (MQ=255)                                            tttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCt                                                                                                                                                                                           >  1:249578‑M2/187‑251 (MQ=255)                                             ttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTTCCTTTTTTCACTTCCt                                                                                                                                                                                           <  2:498748‑M2/65‑1 (MQ=255)                                                atgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTAc                                                                                                                                                                                       <  1:259050‑M2/69‑1 (MQ=255)                                                    taaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACAtt                                                                                                                                                                                    <  1:49137‑M2/72‑1 (MQ=255)                                                     aaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACAttt                                                                                                                                                                                   <  1:267384‑M2/73‑1 (MQ=255)                                                                       gatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTg                                                                                                                                                                >  1:127589‑M2/160‑251 (MQ=255)                                                                        atcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTg                                                                                                                                                                >  2:159662‑M2/159‑250 (MQ=255)                                                                          cacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTGCCTGTACATTTACCCTGTCTGTCCATAGTGAtt                                                                                                                                                             <  1:138910‑M2/95‑1 (MQ=255)                                                                             actatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAAt                                                                                                                                                          >  1:205473‑M2/154‑251 (MQ=255)                                                                                                     aatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCt                                                                                                                                  >  2:277610‑M2/130‑251 (MQ=255)                                                                                                          gagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCttt                                                                                                                                <  2:382700‑M2/124‑1 (MQ=255)                                                                                                                    aaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGc                                                                                                                    <  1:151431‑M2/136‑1 (MQ=255)                                                                                                                        tgtccgttgagcttctaccagcaaattccgatagttgcggcacttcctgagccggaacgaaaagttttatcggaaggcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTTCAATTGGTCTGCGATTCAGGCCATGGTAGCAAAGCTACCTTTTTTCACTGCCTGTACATTTACCCTGTCTGTCCATACTGATTAATGGAGCACCGCCTaattgcggtgcttttatttgccgagcgatc                                                                                                                <  2:92916‑M2/140‑12 (MQ=255)                                                                                                                            cgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCtt                                                                                                                                                                                                       <  1:444706‑M2/53‑1 (MQ=255)                                                                                                                            cgttgagcttctaccagcaaatacctatagtggcggcacttcctgagacggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCtt                                                                                                                                                                                                       >  2:444706‑M2/107‑159 (MQ=255)                                                                                                                               tgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAAtt                                                                                                         <  2:289164‑M2/147‑1 (MQ=255)                                                                                                                                                atacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCa                                                                                       <  1:499039‑M2/165‑1 (MQ=255)                                                                                                                                                atacctatagtggcggcacttcctgagccggaacgaaaagctttatcggaatgcgtggtctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGAGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGaccc                                                                                       >  2:22251‑M2/87‑250 (MQ=255)                                                                                                                                                   cctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGt                                                                                                                                     >  2:140250‑M2/84‑202 (MQ=255)                                                                                                                                                   cctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGt                                                                                                                                     <  1:140250‑M2/119‑1 (MQ=255)                                                                                                                                                                      ctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGtt                                                                 >  2:417032‑M2/65‑251 (MQ=255)                                                                                                                                                                             ggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATctgc                                                          <  2:45756‑M2/194‑1 (MQ=255)                                                                                                                                                                                               gtaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCTACCATCTCTTTCCCGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCg                                        <  1:293530‑M2/212‑1 (MQ=255)                                                                                                                                                                                                       tgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGCTTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCCTCTCTTTCACGTCAGTCCGGTTGATCTGCTGTTTGTTGCCATTAGCGtctttct                                 >  2:510399‑M2/32‑248 (MQ=255)                                                                                                                                                                                                                    ttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCACAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCg                   >  1:183659‑M2/19‑251 (MQ=255)                                                                                                                                                                                                                        ggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCGGTAt               >  1:233647‑M2/15‑251 (MQ=255)                                                                                                                                                                                                                                     gttgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCGGTATCGTTATCAGTCtg  >  2:439456/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                          TAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCGGTATCGTTATCAGTCTG  >  NC_000913/2942544‑2943022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑