Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,948,258	IS3 (–) +5 bp :: +TC	coding (173‑177/339 nt)	maoP →	macrodomain Ori protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	315229 =	NA (NA)	40 (0.850) +TC	35/484	0.4	100%	noncoding (1/1255 nt)	IS3	repeat region
	?	NC_000913	3948258 =	0 (0.000)					coding (173/339 nt)	maoP	macrodomain Ori protein
*	?	NC_000913	1094245 =	NA (NA)	42 (0.880)	40/486	0.3	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	= 3948262	0 (0.000)					coding (177/339 nt)	maoP	macrodomain Ori protein

AACTGAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGAAC  >  NC_000913/314982‑315474                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      tagctatcgagcgattgaccgtttgctcatccatctgcagaaactactgacataaaaaaagggcatttcgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTc                                                                                                                                                                                                                                                      >  1:229094‑M1/249‑250 (MQ=255)  aCTGAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCtt                                                                                                                                                                                                                                                    >  1:412587/1‑250 (MQ=255)     tatcgagcgattgaccgtttgctcatacatctgcagaaaatactgacataaaagaagggcatttcgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggacaacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTAcc                                                                                                                                                                                                                                                 <  1:189328‑M1/7‑1 (MQ=255)          ccAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCaa                                                                                                                                                                                                                                           <  1:308286/251‑1 (MQ=255)             gattgaccgtttgctcatccatctgcagaaactactgacataaaaaaagggcatttcgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAAt                                                                                                                                                                                                                                          <  2:154588‑M1/14‑1 (MQ=255)              attgaccgtttgctcatccatctgcagaaactactgacataaaaaaagggcatttcgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccaaacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAAt                                                                                                                                                                                                                                          <  1:123878‑M1/14‑1 (MQ=255)                tgaccgtttgctcatccatctgcagaaactactgacctaaaaaaagggcatttcgccctttttattaatcgtcagaatcggtgtagtcttcagaaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTg                                                                                                                                                                                                                                      <  1:197762‑M1/18‑1 (MQ=255)                    cgtgtgctaatccatctgcagcaaatacggacataaaaaaagggcatttcgccctttttattaatcgtcagaatcggagtagtattaagcaccttcaacctgaggtttaccgacggaaagggtgtgaaaacgttttggacgcttaatacgcgtcaaatacttggaccacacgcgttctgcttcagtaactggctaacgtacgctacggcatattgctacgaagagttgaTCTTGCCCAGCAATAGTGgacc                                                                                                                                                                                                                                  <  2:440494‑M1/22‑2 (MQ=255)                               ccatctgcagaaactactgacataaaaaaagggcatttcgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAg                                                                                                                                                                                                                        >  2:161344‑M1/219‑250 (MQ=255)                               ccatctgcagaaactactgacataaaaaaagggcatttcgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGt                                                                                                                                                                                                                       <  1:154444‑M1/33‑1 (MQ=255)                                 ggctggAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAg                                                                                                                                                                                                                    <  1:420696/251‑1 (MQ=255)                                     ggAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTaa                                                                                                                                                                                                                 >  2:373993/1‑250 (MQ=255)                                     gcagaatctactgacataaaaaaagggcatttcgcactttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgcctgaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagtttaTCTTACCCAGCAATAGTGGTCACGCGGCTAAGTGa                                                                                                                                                                                                                     >  1:397218‑M1/213‑247 (MQ=255)                                       aaaaaaTCGCAGGATATTGAGGATTATCAGGAACGCCCGGCGACTGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTANAct                                                                                                                                                                                                              >  2:473202/1‑251 (MQ=255)                                          aactactgacataaaaaaagggcatttcgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAActct                                                                                                                                                                                                            >  2:436366‑M1/208‑251 (MQ=255)                                                      aaaaaaagggcatttcgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTg                                                                                                                                                                                                <  1:31285‑M1/56‑1 (MQ=255)                                                               tATCAGGACCGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGGGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATg                                                                                                                                                                                       <  1:440687/250‑1 (MQ=255)                                                                aTCAGGAACGCCCGGCGAATGGCGGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGa                                                                                                                                                                                      <  2:205554/250‑1 (MQ=255)                                                                  tttcgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGAc                                                                                                                                                                                     <  1:243426‑M1/67‑1 (MQ=255)                                                                  tttcgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGAc                                                                                                                                                                                     <  1:55298‑M1/67‑1 (MQ=255)                                                                  tttcgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGAc                                                                                                                                                                                     <  2:447533‑M1/67‑1 (MQ=255)                                                                   ttagccctttttattaatcgtcagaatctgtgtagtctgcagcaccttcaacctgcgggtgaccgccggaaagggtgtgaaaacgtgttggacgcttaagacgcgtcatatacttggaccacacgctttctgcttctgtctctggctcacgttcgccacggcatactgctacgaagagttgaTCGTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGGCAGAGGTGACTCACATGACa                                                                                                                                                                                    >  2:180304‑M1/183‑250 (MQ=255)                                                                       aCGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACa                                                                                                                                                                              <  1:230742/251‑1 (MQ=255)                                                                              tattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATc                                                                                                                                                                         >  1:441700‑M1/172‑250 (MQ=255)                                                                                        gggATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTaaaa                                                                                                                                                              >  1:423487/1‑250 (MQ=255)                                                                                        cagaatcggggtagtcttcagcaccttcaacctgcagtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCATCCAGTaaa                                                                                                                                                               >  2:301636‑M1/162‑250 (MQ=255)                                                                                           agtntcgtgaggagggctggttccccGCCTTGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGGTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAAcc                                                                                                                                                          <  2:465604/228‑1 (MQ=37)                                                                                              cggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAAcccc                                                                                                                                                        <  1:190736‑M1/96‑1 (MQ=255)                                                                                                gtgtagtcttccgcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGCGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGt                                                                                                                                                      <  2:291918‑M1/98‑1 (MQ=255)                                                                                                gtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGt                                                                                                                                                      <  2:62392‑M1/98‑1 (MQ=255)                                                                                                   gCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTAACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAAc                                                                                                                                                  >  1:508250/1‑251 (MQ=255)                                                                                                             aTTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGGATCAACCAGTAAAAAACCCCGTAAACAGCATTCg                                                                                                                                          <  1:452096/249‑1 (MQ=255)                                                                                                                 ttcaacctgcggtttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTg                                                                                                                                      <  1:161934‑M1/114‑1 (MQ=255)                                                                                                                     cccTGGCCACAGCTTGGCGCAAGCCTGATGTATGAACAGTCATATGGCGAGGCAGTCGGGTTGTTTGGTAAAGATAAGCCCCCGAAATGCCCGCATTCTATTTCTGCCGAGGTGCCCTATACGCCGGTGCC‑TCTTTCCCAGCAATAGCGGAGACGCGGATAAGTGAGTAAACCCTCAGTCAGATGTGACTCACATTACCAAAACAGTATCAACCAGTAAAAAACCCCGTAATCACCATTCGCCTGAGTTTc                                                                                                                                <  2:241748/250‑1 (MQ=18)                                                                                                                           ccGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTg                                                                                                                          >  1:101045/1‑251 (MQ=255)                                                                                                                              ttaccgccggaaagggtgtgaaaacgttttggacgcttaatacgcgtcctatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGa                                                                                                                         <  1:35963‑M1/127‑1 (MQ=255)                                                                                                                                   tGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCt                                                                                                                   <  2:336859/250‑1 (MQ=255)                                                                                                                                        cAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAATCCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCt                                                                                                             <  2:508250/251‑1 (MQ=255)                                                                                                                                             cTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTg                                                                                                        >  1:120924/1‑251 (MQ=255)                                                                                                                                                tgaaaacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGa                                                                                                       <  2:178448‑M1/145‑1 (MQ=255)                                                                                                                                                   acacgttttggacgcttaatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggccaacgttcgccacggcatactgctacgaacatttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAACCTCTCAGTCAGAGGTGACTCACATGACAACAACAGTATCAACCAGAACACAACCCCGTAAACAGCATTCGCCTGAATTTCGCTGTGAAGCCCTGAAGCTTGCTGAACGc                                                                                                   <  2:244692‑M1/149‑1 (MQ=255)                                                                                                                                                       aaCAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCgg                                                                                              >  2:83253/1‑251 (MQ=255)                                                                                                                                                          agaattatGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCCTGCTATTTCTTCCGAGGTGACCTATACGCCA‑TGCCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCTGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGAAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAACCTTGTTGAACGCTTCGgtgt                                                                                           >  2:122773/4‑251 (MQ=18)                                                                                                                                                                   taatacgcgtcatatacttggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGc                                                                                     >  2:55298‑M1/87‑249 (MQ=255)                                                                                                                                                                    cGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCa                                                                                 <  1:373993/251‑1 (MQ=255)                                                                                                                                                                               ggCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACt                                                                      >  2:286848/1‑251 (MQ=255)                                                                                                                                                                                      ggaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCt                                                                >  1:5739‑M1/68‑251 (MQ=255)                                                                                                                                                                                       ggTAAAGATAAGCGCGAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACACAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGGGACTCAGATGACAAAAATAGTCTCAACCAGAAAAAATCCCCGTAAACAGCACTCGCCTGAATTTCGCAGCGAAGCCCTTAAGCTTTCTTGACTCATCTGTGTTACTGCCGCAGCCCGTGAACGCAGCCTg                                                               <  2:250676/250‑1 (MQ=18)                                                                                                                                                                                       gaccacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTg                                                               >  2:492406‑M1/67‑251 (MQ=255)                                                                                                                                                                                        accacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGt                                                              >  1:154117‑M1/66‑251 (MQ=255)                                                                                                                                                                                          cacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCagag                                                                                                                                                                                                   <  1:496511‑M1/53‑1 (MQ=255)                                                                                                                                                                                          cacacgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCagag                                                                                                                                                                                                   >  2:496511‑M1/64‑116 (MQ=255)                                                                                                                                                                                          aaaGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCGCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATg                                                           <  1:314024/251‑1 (MQ=255)                                                                                                                                                                                           acacgcgttctgcttctgccactggctcacgttcgccccggcataatgctacgaagagttgaTCTTACCCAGCAATAGAGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGCCTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTACACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGAACCGGTGTTACTGCCGCAGCCCGTGAACTCAGCATGTATg                                                           <  2:210912‑M1/189‑1 (MQ=255)                                                                                                                                                                                            cgcgcgttctgcttctgtcactggctcacgttcgccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGGGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGa                                                          >  1:330254‑M1/62‑251 (MQ=255)                                                                                                                                                                                               tAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACCCGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATc                                                       >  2:346519/1‑250 (MQ=255)                                                                                                                                                                                                      cAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAActct                                               >  1:315691/1‑251 (MQ=255)                                                                                                                                                                                                       aGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAActct                                               >  2:522407/1‑250 (MQ=255)                                                                                                                                                                                                                     gCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGt                                 <  1:473202/250‑1 (MQ=255)                                                                                                                                                                                                                       tATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTaa                               >  1:266973/1‑250 (MQ=255)                                                                                                                                                                                                                             gccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTg                                        >  1:57441‑M1/29‑236 (MQ=255)                                                                                                                                                                                                                             gccacggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTg                                        <  2:57441‑M1/208‑1 (MQ=255)                                                                                                                                                                                                                                  ggcatactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATc                    >  1:154588‑M1/24‑251 (MQ=255)                                                                                                                                                                                                                                  aGGTGACATCTACGCCAGTGCC‑TCCTACCCAGCAATAGTGGACACCCGGCTAAGTGAGTAAACTCTCAGTCAGAGGAGACTCACATGACAAAAACAGTATAAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATc                    >  2:520969/1‑250 (MQ=255)                                                                                                                                                                                                                                    gTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGCAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACCACTGGCGCAGTAAACAGCAAAATcagc                 >  2:20624/1‑251 (MQ=255)                                                                                                                                                                                                                                     atactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATcagc                 >  1:418130‑M1/21‑251 (MQ=255)                                                                                                                                                                                                                                     atactgctacgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATcagc                 >  1:478674‑M1/21‑251 (MQ=255)                                                                                                                                                                                                                                        ccTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACaaaaa                                                                                                                                                                                >  1:112515/1‑88 (MQ=255)                                                                                                                                                                                                                                        ccTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACaaaaa                                                                                                                                                                                <  2:112515/88‑1 (MQ=255)                                                                                                                                                                                                                                             acgaagagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAACACCCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGtc          >  2:200436‑M1/13‑250 (MQ=255)                                                                                                                                                                                                                                                ccAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTg     >  1:357002/1‑251 (MQ=33)                                                                                                                                                                                                                                                 agagttgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCACGTAAACGGCATTCGCCTGAATTTCGCAGTGAAGACCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTg     <  2:16377‑M1/243‑1 (MQ=255)                                                                                                                                                                                                                                                  aGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGaa   <  1:522407/251‑1 (MQ=32)                                                                                                                                                                                                                                                    gttgaTCTTACCCATCAATAGTGCACACGCGGCTAAGTTAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCACCCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCTCAGTGAAGCCCTGAATCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGATCTCAGCCTGTATGAATCACAACTCTCCATCTAGGGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGAAc  >  1:440494‑M1/6‑251 (MQ=255)                                                                                                                                                                                                                                                       c‑tctTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTaa                               <  1:131452/218‑1 (MQ=11)                                                                                                                                                                                                                                                       c‑tctTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTaa                               >  2:131452/1‑218 (MQ=11)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      AACTGAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCC‑TCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGAAC  >  NC_000913/314982‑315474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.