Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 270,496 2 bp→AA pseudogene (219‑220/263 nt) insN → IS911A regulator fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913270,4960GA97.7% 82.2 / ‑3.0 44pseudogene (219/263 nt)insNIS911A regulator fragment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (25/18);  minor base . (1/0);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_000913270,4970CA97.7% 81.2 / NA 44pseudogene (220/263 nt)insNIS911A regulator fragment
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (25/18);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGTCATAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAA‑CTGGTCGTTGACCAGAACTACACCGTGGCAGATGCAGCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGG‑CAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCA‑GGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAG  >  NC_000913/270218‑270761
                                                                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                                                                        
ataaaTTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGANAGCCATGAATGTCGGGCTTTCCACCTNGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGaa                                                                                                                                                                                                                                                      <  2:588120/297‑1 (MQ=9)
           cACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATAtt                                                                                                                                                                                                                                           >  1:61221/1‑300 (MQ=9)
           cACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCACAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATAtt                                                                                                                                                                                                                                           >  2:242097/1‑300 (MQ=9)
           cACCTGAACAGAGGTGATATGCTCACCTCAGAACAACAAAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACTGAAAGCATTCACTATCACGCAGGAACAAATTGAGATATGTGAGCTAAAGGAAAAA‑CTTAAACGTATTGAAATGGaggaggacagatt                                                                                                                                                                                                                                           >  2:296109/1‑288 (MQ=2)
             ccTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATCCGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTaa                                                                                                                                                                                                                                         >  1:288690/1‑300 (MQ=9)
             ccTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTaa                                                                                                                                                                                                                                         >  2:350793/1‑300 (MQ=9)
                gAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTaaaaa                                                                                                                                                                                                                                      >  2:395237/1‑300 (MQ=9)
                gAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTaaaaa                                                                                                                                                                                                                                      >  1:518778/1‑300 (MQ=9)
                gAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAAAGTATTGAAATGGAAAACGAAATATTaaaaa                                                                                                                                                                                                                                      >  1:170052/1‑300 (MQ=9)
                     gagGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAGAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCACTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCt                                                                                                                                                                                                                                 >  2:162053/1‑300 (MQ=9)
                     gagGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTagaagcggct                                                                                                                                                                                                                                 >  2:121364/1‑291 (MQ=9)
                     gagGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCt                                                                                                                                                                                                                                 >  1:192868/1‑300 (MQ=9)
                     gagGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCt                                                                                                                                                                                                                                 >  2:162209/1‑300 (MQ=9)
                       ggTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCAGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAANGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTAc                                                                                                                                                                                                                               >  2:192843/1‑300 (MQ=9)
                        gTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGN‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACt                                                                                                                                                                                                                              >  1:619448/1‑300 (MQ=9)
                        gTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTAcc                                                                                                                                                                                                                              >  2:409520/1‑299 (MQ=9)
                        gTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGC‑CAAGCTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCAACTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCAGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTAcc                                                                                                                                                                                                                              >  2:368482/1‑299 (MQ=255)
                                         gAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCa                                                                                                                                                                                                             >  1:382453/1‑300 (MQ=255)
                                         gAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCACTATCACGCCGGAACAAATTGAGCTACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAGACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCa                                                                                                                                                                                                             >  2:745400/1‑300 (MQ=255)
                                             aacaCAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGc                                                                                                                                                                                                         <  1:22278/300‑1 (MQ=255)
                                              acacAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCa                                                                                                                                                                                                        <  1:514097/300‑1 (MQ=255)
                                                  aggtgccttAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACac                                                                                                                                                                                                    <  1:489140/292‑2 (MQ=2)
                                                            aTGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACTGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACacccctttcaat                                                                                                                                                                                          <  1:344271/300‑12 (MQ=2)
                                                              gAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTTACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGACATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAAc                                                                                                                                                                                        <  2:543435/300‑1 (MQ=255)
                                                                                                       gcccagctggttgtggatcAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACGGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACCTTTAc                                                                                                                                                >  2:664452/19‑300 (MQ=12)
                                                                                                         ccagctggttgtggatcAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAcag                                                                                                                                              <  2:372123/284‑1 (MQ=12)
                                                                                                          cagctggttgtggatcAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAcagc                                                                                                                                             <  2:310243/285‑1 (MQ=12)
                                                                                                                       atcAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGcc                                                                                                                                <  2:551442/298‑1 (MQ=12)
                                                                                                                                     gttgcagatgccgcgaaagccatgaaTGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACt                                                                                                                  >  2:11385/26‑300 (MQ=12)
                                                                                                                                        gcagatgccgcgaaagccatgaaTGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAAAGTATTGAAATGGAAAACGAAATATTAAAAAAGGGTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGGGGTTTTTTGACTGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATg                                                                                                               >  2:514408/23‑300 (MQ=12)
                                                                                                                                              gccgcgaaagccatgaaTGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACNAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGgaagaa                                                                                                         <  1:641909/284‑1 (MQ=17)
                                                                                                                                                             aaTGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCACTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAg                                                                                          >  1:33726/2‑300 (MQ=17)
                                                                                                                                                              aTGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAAAAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACGACAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGt                                                                                         >  2:813293/1‑300 (MQ=17)
                                                                                                                                                                                   cgcgGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGt                                                                    <  2:452694/300‑1 (MQ=17)
                                                                                                                                                                                           ggtaaagcaattactggacgaacgggcaggCAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGNAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAANCATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAAc                                                             <  2:689216/272‑1 (MQ=17)
                                                                                                                                                                                                   aTTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGa                                                    >  1:645409/1‑300 (MQ=17)
                                                                                                                                                                                                                     caggCAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACTAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAgg                                   <  2:184103/298‑1 (MQ=14)
                                                                                                                                                                                                                     caggCAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAgg                                   <  1:227294/298‑1 (MQ=17)
                                                                                                                                                                                                                      aggCAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAggg                                  <  1:750770/299‑1 (MQ=17)
                                                                                                                                                                                                                       ggCAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGa                                 <  1:426463/300‑1 (MQ=17)
                                                                                                                                                                                                                        gCAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATTAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGAt                                <  1:353017/300‑1 (MQ=17)
                                                                                                                                                                                                                                         tcccctatcacgccGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAAcc               >  1:733615/13‑300 (MQ=14)
                                                                                                                                                                                                                                           ccctatcacgccGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAAccac             >  1:221977/11‑298 (MQ=14)
                                                                                                                                                                                                                                                      cGGAACAAATTGAGATACGTGAGCGGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTTAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAg  <  2:379510/300‑1 (MQ=14)
                                                                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                                                                        
CTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGTCATAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAA‑CTGGTCGTTGACCAGAACTACACCGTGGCAGATGCAGCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGG‑CAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCA‑GGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAG  >  NC_000913/270218‑270761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑