Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	270,400	A→G	pseudogene (123/263 nt)	insN →	IS911A regulator fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	270,400	0	A	G	100.0%	83.8 / NA	52	pseudogene (123/263 nt)	insN	IS911A regulator fragment
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/14);  total (38/14)

TCATGAATCCTTTTCACTAGGGATGAAGTGGTTTACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGTCATAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAA‑CTGGTCGTTGACCAGAACTACACCGTGGCAGATGCAGCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGG‑CAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCA‑GGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGT  >  NC_000913/270183‑270695                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                          aaaagtgtcattgatactaaccttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTg                                                                                                                                                                                                                        <  1:126061/274‑1 (MQ=2)  aagctacatgaagcgatgcctttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGa                                                                                                                                                                                                                       >  1:277271/26‑300 (MQ=2)    gctacatgaagcgatgcctttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGc                                                                                                                                                                                                                     >  1:817217/24‑300 (MQ=9)     gtgtcattgatactaaccttttaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACATGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCt                                                                                                                                                                                                                    <  1:296553/278‑1 (MQ=9)     gtgtcattgatactaaccttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCt                                                                                                                                                                                                                    <  1:302724/278‑1 (MQ=9)       acatgaagcgatgcctttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCACTATCACGCCGGAACAAATTGAGATACGTGAGCtga                                                                                                                                                                                                                  >  1:329305/21‑298 (MQ=2)         cattgatactaaccttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCtgaag                                                                                                                                                                                                                >  1:839355/19‑296 (MQ=9)             gatactaaccttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGaaaa                                                                                                                                                                                                           >  1:690065/15‑300 (MQ=9)              atactaaccttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCTAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCACTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGaaaaa                                                                                                                                                                                                          >  1:772792/14‑300 (MQ=9)                   gcctttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGaaaaaaattc                                                                                                                                                                                                     >  1:96875/9‑295 (MQ=9)                    cctttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCCCGCCGGAACAAATTGAGATACGTGAGCTGA‑AGaaaaaaattca                                                                                                                                                                                                    >  2:333103/8‑294 (MQ=2)                    cctttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGaaaaaaattca                                                                                                                                                                                                    >  1:728455/8‑294 (MQ=9)                    cctttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCAGGAACAAATTGAGATACGTGAGCTGA‑AGaaaaaaattca                                                                                                                                                                                                    >  2:814596/8‑294 (MQ=2)                    accttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGaaaaaaattca                                                                                                                                                                                                    >  1:712828/8‑294 (MQ=9)                    accttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGaaaaaaattca                                                                                                                                                                                                    >  2:772182/8‑294 (MQ=9)                    accttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCACTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGaaaaaaattca                                                                                                                                                                                                    >  1:163494/8‑294 (MQ=2)                       ttgtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGaaaaaaattcaacg                                                                                                                                                                                                 >  1:111210/5‑291 (MQ=9)                         gtaGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGACTGTTGGGCTTTCCACCTTGACGCGGCGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAAAACCGAAAGCATCCACTATGACGCCGGAACAAATTGAGATAAGTGAGCtgacgaacaaaattcaacgta                                                                                                                                                                                                >  2:74110/3‑280 (MQ=2)                                    ataaaTTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGANAGCCATGAATGTCGGGCTTTCCACCTNGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGaa                                                                                                                                                                                    <  2:588120/297‑1 (MQ=9)                                               cACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATAtt                                                                                                                                                                         >  1:61221/1‑300 (MQ=9)                                               cACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCACAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATAtt                                                                                                                                                                         >  2:242097/1‑300 (MQ=9)                                               cACCTGAACAGAGGTGATATGCTCACCTCAGAACAACAAAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACTGAAAGCATTCACTATCACGCAGGAACAAATTGAGATATGTGAGCTAAAGGAAAAA‑CTTAAACGTATTGAAATGGaggaggacagatt                                                                                                                                                                         >  2:296109/1‑288 (MQ=2)                                                 ccTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATCCGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTaa                                                                                                                                                                       >  1:288690/1‑300 (MQ=9)                                                 ccTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTaa                                                                                                                                                                       >  2:350793/1‑300 (MQ=9)                                                    gAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTaaaaa                                                                                                                                                                    >  2:395237/1‑300 (MQ=9)                                                    gAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTaaaaa                                                                                                                                                                    >  1:518778/1‑300 (MQ=9)                                                    gAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAAAGTATTGAAATGGAAAACGAAATATTaaaaa                                                                                                                                                                    >  1:170052/1‑300 (MQ=9)                                                         gagGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAGAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCACTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCt                                                                                                                                                               >  2:162053/1‑300 (MQ=9)                                                         gagGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTagaagcggct                                                                                                                                                               >  2:121364/1‑291 (MQ=9)                                                         gagGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCt                                                                                                                                                               >  1:192868/1‑300 (MQ=9)                                                         gagGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCt                                                                                                                                                               >  2:162209/1‑300 (MQ=9)                                                           ggTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCAGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAANGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTAc                                                                                                                                                             >  2:192843/1‑300 (MQ=9)                                                            gTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGN‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACt                                                                                                                                                            >  1:619448/1‑300 (MQ=9)                                                            gTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTAcc                                                                                                                                                            >  2:409520/1‑299 (MQ=9)                                                            gTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGC‑CAAGCTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCAACTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCAGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTAcc                                                                                                                                                            >  2:368482/1‑299 (MQ=255)                                                                             gAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCa                                                                                                                                           >  1:382453/1‑300 (MQ=255)                                                                             gAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCACTATCACGCCGGAACAAATTGAGCTACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAGACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCa                                                                                                                                           >  2:745400/1‑300 (MQ=255)                                                                                 aacaCAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGc                                                                                                                                       <  1:22278/300‑1 (MQ=255)                                                                                  acacAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCa                                                                                                                                      <  1:514097/300‑1 (MQ=255)                                                                                      aggtgccttAATGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACac                                                                                                                                  <  1:489140/292‑2 (MQ=2)                                                                                                aTGAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACTGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACacccctttcaat                                                                                                                        <  1:344271/300‑12 (MQ=2)                                                                                                  gAAAAAACGAAATTTCAGTGCAGAATTCAGACGTGAATCAGCCCAG‑CTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTTACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGACATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAAc                                                                                                                      <  2:543435/300‑1 (MQ=255)                                                                                                                                           gcccagctggttgtggatcAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACGGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACCTTTAc                                                                              >  2:664452/19‑300 (MQ=12)                                                                                                                                             ccagctggttgtggatcAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAcag                                                                            <  2:372123/284‑1 (MQ=12)                                                                                                                                              cagctggttgtggatcAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAcagc                                                                           <  2:310243/285‑1 (MQ=12)                                                                                                                                                           atcAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGcc                                                              <  2:551442/298‑1 (MQ=12)                                                                                                                                                                         gttgcagatgccgcgaaagccatgaaTGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACt                                                >  2:11385/26‑300 (MQ=12)                                                                                                                                                                            gcagatgccgcgaaagccatgaaTGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAAAGTATTGAAATGGAAAACGAAATATTAAAAAAGGGTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGGGGTTTTTTGACTGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATg                                             >  2:514408/23‑300 (MQ=12)                                                                                                                                                                                  gccgcgaaagccatgaaTGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACNAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGgaagaa                                       <  1:641909/284‑1 (MQ=17)                                                                                                                                                                                                 aaTGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCACTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAg                        >  1:33726/2‑300 (MQ=17)                                                                                                                                                                                                  aTGTCGGGCTTTCCACCTTGACGCGGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAAAAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACGACAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGt                       >  2:813293/1‑300 (MQ=17)                                                                                                                                                                                                                       cgcgGTGGGTAAAGCAATTACGGGACGAACGGGCAGG‑CAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGA‑AGAAAAAAATTCAACGTATTGAAATGGAAAACGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGt  <  2:452694/300‑1 (MQ=17)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                          TCATGAATCCTTTTCACTAGGGATGAAGTGGTTTACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGTCATAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAA‑CTGGTCGTTGACCAGAACTACACCGTGGCAGATGCAGCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGG‑CAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCA‑GGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGT  >  NC_000913/270183‑270695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑