Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,494	T→A	intergenic (+665/+71)	ycdU → / ← serX	uncharacterized protein YcdU/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,494	0	T	A	90.9%	111.8 / 7.6	46	intergenic (+665/+71)	ycdU/serX	uncharacterized protein YcdU/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base A (35/5);  total (37/9)
Fisher's exact test for biased strand distribution p-value = 1.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTT  >  NC_000913/1097217‑1097789                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                        ggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTNCACTCGCCCTGCTGGCAGCCCTCTCACTGCGTTCCCAGTCTGTCCAACTGGCTGTCGACAGTTGTCGCACCCCGGTCGTGGCATCCCATCCCCCAGGtgtgtgaa                                                                                                                                                                                                                                                                                   >  2:785986/1‑298 (MQ=11)   aTTGACTCGTTTCGCTCGCCCTGCGGGCAGCTCGTTCATTGCGTTCACGTTCTGTCTAAATGGCTGGCGCCGGTTGTCGAACCCCGTTCGGGGATTCTTGTCCCCCCGGTGGGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGTTCGGGGCTTCTCATCCCCCCGGTGTGTGCAat                                                                                                                                                                                                                                                                                 <  1:472460/300‑1 (MQ=11)   aTTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAat                                                                                                                                                                                                                                                                                 <  1:86392/300‑1 (MQ=32)                                            cGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCttt                                                                                                                                                                                                                                        >  1:380715/1‑300 (MQ=17)                                                   ggTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCNACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATCTACGAAAAAAAAGCACGTACTTTCGTACGAGCTCTTCTTTAAATATg                                                                                                                                                                                                                                 >  1:626077/1‑300 (MQ=17)                                                   ggTCTGTCCAACTGGCTGTCGACAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTATTTAAATATGGCGGTGAGGGGGGGGTTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGTATCTCATACACCAGGTGTGTGTAAGATCCGAACAAAAAGCCCGTACTTTCGTATGAGCGCTTATTTCAatata                                                                                                                                                                                                                                 >  2:330912/1‑299 (MQ=11)                                                     tccttCCAACTGGCTTTCGCCAGTTGTTGAACCCCGTTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGTTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACTAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGc                                                                                                                                                                                                                               <  1:298659/296‑1 (MQ=255)                                                        gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTCAATATGGCGGt                                                                                                                                                                                                                            >  1:147618/1‑300 (MQ=17)                                                        gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCAGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTATTTAAATATGGAGGt                                                                                                                                                                                                                            >  1:845184/1‑300 (MQ=12)                                                        gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCACCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCCACTGGCTGTCGCCAGTTGTCGAACCACGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTAGTACGAGCTCTCCTTTACATCTGGCgtt                                                                                                                                                                                                                            >  2:184214/1‑298 (MQ=11)                                                        gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCACTCGCCCCGCGGGCCGCCCTCTCACTGCGTTCCCGTTCTGTCCCCCTGGCTGCCACCAGCTTTCTAACCCCGGTCGTGACGGCTCATCCACCAGGTGTGTGCAATATCCGAAAAAAACAACCGTACTTTCGTACAAACTCTTCtttccatctgacggt                                                                                                                                                                                                                            >  1:747808/1‑288 (MQ=11)                                                                                        ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCCATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGTGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCACCTGGCTGTCGCCAGTTGTCGAACCCAGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTCCATATAGCGGTGAGGGGGGGATTCGCACCCCCGATACGTTGcc                                                                                                                                                                                            >  1:827008/1‑300 (MQ=11)                                                                                             gggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGtata                                                                                                                                                                                       >  1:716823/1‑300 (MQ=255)                                                                                              ggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAACATGTCGGTGAGGGGGGGATTGACTCGCTTCGCCCGCCCTTCTTGCCGCCCGCTCACTTCGTTCACTGTCCTTCCAACTTGCTGTCGCCATTTTGCGAATTTTGTTCGGGGCTTTTTATCCCCCCCAGGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATac                                                                                                                                                                                      <  1:692934/300‑1 (MQ=11)                                                                                              ggCTTCCCACCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCCCTTCTCTAAATATGTCTGTGGGGGGGGGATTGACTCTCTTCGCTCGCCCTGCGGGTAGCCCGCTCGCTGCNTTCGCGGTCTGTCCAACTGGCTGGCGCCAGTTTTCGAACCCCGGTCGGGGCGTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATac                                                                                                                                                                                      <  2:226262/300‑1 (MQ=14)                                                                                                cTTCTCATCCCCCCGGTGTGGGCAATATACGAAAAAAAAGCCCGTACTTTCGTANGAGCTCGTCTGTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATacac                                                                                                                                                                                    <  2:674175/300‑1 (MQ=255)                                                                                                       tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTcc                                                                                                                                                                             >  1:492829/1‑300 (MQ=255)                                                                                                         cccccGGTGTGTGCAATATACGAAAAAAAAGNCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTNACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGACCCCCCGATACGTTGCCGTCTACACCCTTTCCAg                                                                                                                                                                           >  2:636833/1‑300 (MQ=255)                                                                                                         cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAg                                                                                                                                                                           >  1:272758/1‑300 (MQ=255)                                                                                                         cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGACAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCCTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCAATACGTTGCCGTATACACACTTTCCAg                                                                                                                                                                           >  2:332604/1‑300 (MQ=255)                                                                                                                 gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTc                                                                                                                                                                   >  1:53599/1‑300 (MQ=255)                                                                                                                 gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGACAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCACCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTATGAGCTCTTATTTACATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTc                                                                                                                                                                   >  1:120581/1‑300 (MQ=255)                                                                                                                   gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTCAATCTGGCGGTGAGGGGGGGATTAGAACCCCCGATCCGTTGCCGTCTACACCCTTTCCAGGTGTGCTCCt                                                                                                                                                                 >  2:522044/1‑300 (MQ=14)                                                                                                                   gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCt                                                                                                                                                                 >  1:322328/1‑300 (MQ=255)                                                                                                                         tataCGAAAAAAAAGCCCGTACTTTCATACGAGCGCCTCTTTAAATACGGCTGCGAGTCTTGAATCGACTCTCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGTGTTCACGGTCTGTCCGACTGGCTGTCGCCAGCTGTCGAACTCCGGTCGGGGCATCGCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGcc                                                                                                                                                           <  2:276740/300‑1 (MQ=17)                                                                                                                                 aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGac                                                                                                                                                   >  1:394731/1‑300 (MQ=255)                                                                                                                                                              tcttTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGc                                                                                                                      >  1:871388/1‑300 (MQ=255)                                                                                                                                                              tcttTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCAAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCCGTCTTGc                                                                                                                      >  2:70182/1‑300 (MQ=255)                                                                                                                                                                              tGAGGGGGGGATTAACTCGCTTCGCTCGCCCTGCGTGCAGCCCGCTCACTGCGTTCACGGTCCTTCCAACTAGCTTTCGCCAGTTGTCGACCTCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAt                                                                                                      <  1:444800/300‑1 (MQ=21)                                                                                                                                                                               gcgggggggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTAATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAtt                                                                                                     >  1:222526/3‑300 (MQ=255)                                                                                                                                                                               gAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCAGCTCACTGCGTTCACGGTCTGTCCAACTGGCTATCGCCAGTTGTCGACCCCCGGTCGGGGCATCTAATCCCACCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTCGCAGTATACACACCTTCCAGGCGTGCTCCTTCAGCCAATCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCCGGAACGGGCGCTAAtc                                                                                                     >  2:47964/1‑299 (MQ=18)                                                                                                                                                                                      ggATTGACTCGCTTCGCTCGCCCTGCGGGCANCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCACGGTGTGTGCAATATANGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaa                                                                                              >  2:654128/1‑300 (MQ=255)                                                                                                                                                                                                         gcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTAACCAAATTGTCGNTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGANGTcaac                                                                           >  2:737893/1‑300 (MQ=255)                                                                                                                                                                                                            cTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATTTCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTAACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACaaa                                                                        >  1:298653/1‑300 (MQ=255)                                                                                                                                                                                                            cTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACaaa                                                                        >  1:299708/1‑300 (MQ=255)                                                                                                                                                                                                                                  gTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGTTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTNAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGtt                                                  >  1:648163/1‑300 (MQ=255)                                                                                                                                                                                                                                   ttCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGGGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGACCCCCCGATACGTTGCAGTATACACACTTTCCAGGCGTGCTCATTCCGCCACTCGGACACCTCACCAACTTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGCAATCATGACCTGAGGTCAACACACTTTTTGAAAAAATCGCGCGttt                                                 >  2:75504/1‑300 (MQ=25)                                                                                                                                                                                                                                   ttCACGGTCTGTCCAACTGGCTGTCGACAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCNCTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAACTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGttt                                                 >  2:779593/1‑300 (MQ=255)                                                                                                                                                                                                                                           cTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCNGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAAc                                         >  1:612839/1‑300 (MQ=255)                                                                                                                                                                                                                                           cTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAAc                                         >  1:721185/1‑300 (MQ=255)                                                                                                                                                                                                                                             gTCCAACTGGCTGTCGCCAGTTGTCGAACCCNGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGNGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACtt                                       >  2:651388/1‑300 (MQ=255)                                                                                                                                                                                                                                             gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACtt                                       >  1:153891/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGt                        <  1:420011/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   gTCGAACCCCGGTCGGGGCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTNTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCACACTTCAATCCATGTGTGGTTTtaata                 >  2:795534/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gggCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTATGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAACTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATGAGCGAAGTCTGCttt  >  2:299796/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gggCATCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATCAGCGAAATCTGCttt  >  2:56937/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                        GGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTT  >  NC_000913/1097217‑1097789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑