Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,132,697	G→A	E28K (GAG→AAG)	metF →	5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,132,697	0	G	A	100.0%	339.7 / NA	92	E28K (GAG→AAG)	metF	5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/64);  total (28/64)

GTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCGAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGT  >  NC_000913/4132404‑4132969                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                 gTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGtttt                                                                                                                                                                                                                                                                            >  1:120851/1‑300 (MQ=255)     atatTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTccc                                                                                                                                                                                                                                                                        <  1:520091/300‑1 (MQ=255)      tatTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCGTTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGCTAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTAGTTCAAGTTTTTCccg                                                                                                                                                                                                                                                                       >  2:88668/1‑300 (MQ=255)              gTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTAc                                                                                                                                                                                                                                                               >  2:112132/1‑300 (MQ=255)               tCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTAcc                                                                                                                                                                                                                                                              <  1:178622/300‑1 (MQ=255)                 cGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAg                                                                                                                                                                                                                                                            <  1:211043/300‑1 (MQ=255)                      tgctttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATATTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGaaa                                                                                                                                                                                                                                                       <  1:721575/300‑1 (MQ=255)                         tttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATgg                                                                                                                                                                                                                                                    <  1:578664/300‑1 (MQ=255)                            gcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGTTTGACGCCCTTCGGCTTTTCCTTCANCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGc                                                                                                                                                                                                                                                 <  1:619533/300‑1 (MQ=255)                            gcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGc                                                                                                                                                                                                                                                 <  1:151768/300‑1 (MQ=255)                                 ccGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGAcc                                                                                                                                                                                                                                            <  1:564256/300‑1 (MQ=255)                                  cGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGTTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGAccc                                                                                                                                                                                                                                           <  1:40978/300‑1 (MQ=255)                                  cGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGAccc                                                                                                                                                                                                                                           <  1:311928/300‑1 (MQ=255)                                   ggATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCATGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCt                                                                                                                                                                                                                                          >  2:40696/1‑300 (MQ=255)                                       tttctttttcttACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTgg                                                                                                                                                                                                                                      <  1:13619/300‑1 (MQ=255)                                        ttctttttcttACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGNCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATNAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGa                                                                                                                                                                                                                                     <  2:680858/300‑1 (MQ=255)                                          ctttttcttACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAAc                                                                                                                                                                                                                                   <  1:824274/300‑1 (MQ=255)                                          ctttttcttACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGAGAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAAc                                                                                                                                                                                                                                   <  2:482071/300‑1 (MQ=255)                                            ttttcttACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTc                                                                                                                                                                                                                                 <  2:777091/300‑1 (MQ=255)                                            ttttcttACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTc                                                                                                                                                                                                                                 <  1:517582/300‑1 (MQ=255)                                            ttttcttACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGGCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTc                                                                                                                                                                                                                                 <  1:153044/300‑1 (MQ=255)                                               tcttACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCat                                                                                                                                                                                                                              <  1:562286/300‑1 (MQ=255)                                                    ccATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCatcgatc                                                                                                                                                                                                                         <  1:742971/300‑1 (MQ=255)                                                              gTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGANGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCNCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTagcag                                                                                                                                                                                                               <  2:790075/300‑1 (MQ=255)                                                              gTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTagcag                                                                                                                                                                                                               <  1:235129/300‑1 (MQ=255)                                                                      aTCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCNCTGAATCAGAGCCTGGCAGAAGACCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGNAACTCCATCGATCGCCTTAGCAGCCTGAAAc                                                                                                                                                                                                       <  2:600956/300‑1 (MQ=255)                                                                      aTCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGGTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAAc                                                                                                                                                                                                       <  2:241333/300‑1 (MQ=255)                                                                      aTCTTCCCTGATTTTTCCTCACCATCATTGGTCAGTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATTAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAAc                                                                                                                                                                                                       <  2:386717/300‑1 (MQ=255)                                                                         ttCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGa                                                                                                                                                                                                    >  2:506556/1‑300 (MQ=255)                                                                                   tttCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATc                                                                                                                                                                                          <  1:425928/300‑1 (MQ=255)                                                                                         cACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGAc                                                                                                                                                                                    >  2:499963/1‑300 (MQ=255)                                                                                             atcatTGTTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTAt                                                                                                                                                                                <  2:327778/300‑1 (MQ=255)                                                                                             atcatTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTAt                                                                                                                                                                                <  1:287610/300‑1 (MQ=255)                                                                                               catgggTCATTTTTCGGTTGACGCTCGTCGGCTTTTCCTGCATCTGTACATCTGGACGTCTAAATGGATAGAGGTGCACAACACAACATATAACTACAAGCGATTGATGAAGTAAGGTATGAGCTTTCTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGTCTGGCAGAAGGCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCGGTGAAGGGGAGCAGACCCTGTGAAACTCCATCGATCGCTTTAGCAGCCGGAAACCGAAGTTTGTATCGGTGACCTaggg                                                                                                                                                                              <  2:400578/296‑4 (MQ=255)                                                                                               catTGTTCATTTTTCGTTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATgg                                                                                                                                                                              <  2:399390/300‑1 (MQ=255)                                                                                               catTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATgg                                                                                                                                                                              <  1:279978/300‑1 (MQ=255)                                                                                                 ttGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGNTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGgcg                                                                                                                                                                            <  1:580681/300‑1 (MQ=255)                                                                                                  tGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGgcgc                                                                                                                                                                           <  1:665813/300‑1 (MQ=255)                                                                                                             cGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCg                                                                                                                                                                <  1:449555/300‑1 (MQ=255)                                                                                                               gTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAg                                                                                                                                                              >  2:361604/1‑300 (MQ=255)                                                                                                               gTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAg                                                                                                                                                              >  1:826972/1‑300 (MQ=255)                                                                                                                       ccTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGTATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATTGCGCGAACTCCGGCGAGCGCGACCg                                                                                                                                                      <  1:799705/300‑1 (MQ=255)                                                                                                                            ggCTTTNCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACANAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGc                                                                                                                                                 <  2:716929/300‑1 (MQ=255)                                                                                                                              cTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGTTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTTAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGTTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGcac                                                                                                                                               <  1:420148/300‑1 (MQ=255)                                                                                                                              cTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGNGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTNCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGcac                                                                                                                                               <  2:699226/300‑1 (MQ=255)                                                                                                                              cTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGcac                                                                                                                                               <  1:321184/300‑1 (MQ=255)                                                                                                                                 ttCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGc                                                                                                                                            <  1:675264/300‑1 (MQ=255)                                                                                                                                 ttCCTTCATCTTTACATCTGGACGTCGAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAGCGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGc                                                                                                                                            <  2:415137/300‑1 (MQ=255)                                                                                                                                        aTCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGNATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGNACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCAttatta                                                                                                                                     <  2:679520/300‑1 (MQ=255)                                                                                                                                        aTCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCAttatta                                                                                                                                     <  1:199396/300‑1 (MQ=255)                                                                                                                                           tttACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTTGCAGAAGTCCAGGGGCAGATTAACGTNTCGTTCAAGTTTTTCCCGCCGCGTACNAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAg                                                                                                                                  <  2:584343/300‑1 (MQ=255)                                                                                                                                                   tGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTaaa                                                                                                                          <  1:738956/300‑1 (MQ=255)                                                                                                                                                    ggACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAg                                                                                                                         <  1:694619/300‑1 (MQ=255)                                                                                                                                                                ggATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTc                                                                                                             >  1:196301/1‑300 (MQ=255)                                                                                                                                                                ggATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTc                                                                                                             >  2:415195/1‑300 (MQ=255)                                                                                                                                                                     gATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTTGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCATTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGaa                                                                                                        <  1:230726/300‑1 (MQ=255)                                                                                                                                                                     gATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGaa                                                                                                        >  2:108079/1‑300 (MQ=255)                                                                                                                                                                     gATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGaa                                                                                                        >  1:137383/1‑300 (MQ=255)                                                                                                                                                                     gATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAAGCATTAAAGATCGCACTGGTCTGGaa                                                                                                        >  1:237884/1‑300 (MQ=255)                                                                                                                                                                     gATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTAGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTCTTAAAGGCATTAAAGATCGCACTGGTCTGGaa                                                                                                        >  2:373175/1‑300 (MQ=255)                                                                                                                                                                      aTGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAg                                                                                                       <  2:368429/300‑1 (MQ=255)                                                                                                                                                                            acaacacaacATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCAc                                                                                                 <  1:499557/300‑1 (MQ=255)                                                                                                                                                                             caacacaacaTATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTTAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCAcc                                                                                                >  1:802000/1‑300 (MQ=255)                                                                                                                                                                             caacacaacaTATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCAcc                                                                                                >  1:204331/1‑300 (MQ=255)                                                                                                                                                                             caacacaacaTATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGAACTATGGCGCGAACTCCGGCGAGCGCGACCGGACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCAcc                                                                                                >  1:802618/1‑300 (MQ=255)                                                                                                                                                                               acacaacaTATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCAcagc                                                                                              >  2:178935/1‑297 (MQ=255)                                                                                                                                                                               acacaacaTATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGc                                                                                              >  2:473741/1‑300 (MQ=255)                                                                                                                                                                               acacaacaTATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGc                                                                                              >  1:402697/1‑300 (MQ=255)                                                                                                                                                                                      atatAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGTGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTAc                                                                                       <  2:297393/300‑1 (MQ=255)                                                                                                                                                                                      atatAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTNTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTAc                                                                                       <  2:746734/300‑1 (MQ=255)                                                                                                                                                                                           aCTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCa                                                                                  <  1:748681/300‑1 (MQ=255)                                                                                                                                                                                            cTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCAt                                                                                 <  1:721719/300‑1 (MQ=255)                                                                                                                                                                                            cTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCAt                                                                                 >  2:285005/1‑300 (MQ=255)                                                                                                                                                                                                 aGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTNTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGagg                                                                            <  2:763906/300‑3 (MQ=255)                                                                                                                                                                                                             ggtaaggtaTGAGCTTTTTTCACGCCAGCCGGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCcgacg                                                                >  1:346482/1‑300 (MQ=255)                                                                                                                                                                                                             ggtaaggtaTGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCcgacg                                                                >  1:108118/1‑300 (MQ=255)                                                                                                                                                                                                             ggtaaggtaTGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTGAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGNACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCcgacg                                                                >  2:752024/1‑300 (MQ=255)                                                                                                                                                                                                                               ttCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCCCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCAc                                              <  2:523362/300‑1 (MQ=255)                                                                                                                                                                                                                                             cGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAAt                                <  1:35173/300‑1 (MQ=255)                                                                                                                                                                                                                                               ggATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATaa                              <  1:102935/300‑1 (MQ=255)                                                                                                                                                                                                                                                 aTGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACg                            <  1:19342/300‑1 (MQ=255)                                                                                                                                                                                                                                                  tGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACgg                           <  1:112604/300‑1 (MQ=255)                                                                                                                                                                                                                                                    cccTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTa                         >  1:705185/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCg                     <  1:807433/300‑1 (MQ=255)                                                                                                                                                                                                                                                        gAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCATGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGAACGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCg                     >  2:111518/1‑300 (MQ=255)                                                                                                                                                                                                                                                          aTCAGANCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCNGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTc                   <  2:704673/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aTCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTc                   >  2:58183/1‑300 (MQ=255)                                                                                                                                                                                                                                                               agCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATc              <  2:120326/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      cAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGgcgc       <  1:852204/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      cAGAAGTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGNTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAANGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGgcgc       <  2:690647/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      cAGAAGTCCAGGGGCAAATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCATTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGgcgc       <  2:366197/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           gTCCAGGGGCAGATTAACGTTTCGTTCAAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGt  >  1:176625/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                 GTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAACATATAACTACAAGCGATTGATGAGGTAAGGTATGAGCTTTTTTCACGCCAGCCAGCGGGATGCCCTGAATCAGAGCCTGGCAGAAGTCCAGGGGCAGATTAACGTTTCGTTCGAGTTTTTCCCGCCGCGTACCAGTGAAATGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGT  >  NC_000913/4132404‑4132969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑