Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,548,392	T→C	D560G (GAT→GGT)	malQ ←	4‑alpha‑glucanotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,548,392	0	T	C	98.3%	218.1 / NA	59	D560G (GAT→GGT)	malQ	4‑alpha‑glucanotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (35/23);  total (35/24)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCT  >  NC_000913/3548097‑3548683                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                    gTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCATCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTAATCCGGATACAGCCCCAGGGTTTTGCCCAGCGCTAGACcccc                                                                                                                                                                                                                                                                                                 >  1:129063/1‑300 (MQ=255)     ttATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCAc                                                                                                                                                                                                                                                                                             >  1:663085/1‑300 (MQ=255)                aCTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAAcc                                                                                                                                                                                                                                                                                  >  1:670040/1‑300 (MQ=255)                 cTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACcg                                                                                                                                                                                                                                                                                 <  1:310769/300‑1 (MQ=255)                                       gTTCGGCCATATCCAGCCAGTCTTCCGGTTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCAtgtg                                                                                                                                                                                                                                                           <  2:458264/300‑1 (MQ=255)                                       gTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCGAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCAGCGACATCAACGATGCCTTGTGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCAtgtg                                                                                                                                                                                                                                                           <  2:458270/300‑1 (MQ=255)                                           ggCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTcgc                                                                                                                                                                                                                                                       >  2:119141/1‑300 (MQ=255)                                            gCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTcgcc                                                                                                                                                                                                                                                      >  1:116432/1‑300 (MQ=255)                                                        cagTCTTCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCTTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATNCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGNTGGCAGGTCATGTGTCGCCGCAACCGCCATc                                                                                                                                                                                                                                          <  2:636653/300‑1 (MQ=255)                                                              tCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGc                                                                                                                                                                                                                                    <  1:692715/300‑1 (MQ=255)                                                                cGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTc                                                                                                                                                                                                                                  <  1:255466/300‑1 (MQ=255)                                                                       aGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATAc                                                                                                                                                                                                                           >  1:437584/1‑300 (MQ=255)                                                                            tAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCCCTTCATCCGGATACAGCACAAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCttt                                                                                                                                                                                                                      >  2:527520/1‑300 (MQ=255)                                                                                 gggCACTGTTGCTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGGCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTg                                                                                                                                                                                                                 <  2:54648/298‑1 (MQ=255)                                                                                      cTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACgg                                                                                                                                                                                                            >  1:217074/1‑300 (MQ=255)                                                                                      cTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTACACgg                                                                                                                                                                                                            >  2:139269/1‑300 (MQ=255)                                                                                      cTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCACCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACgg                                                                                                                                                                                                            >  2:571621/1‑300 (MQ=255)                                                                                                  gCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCg                                                                                                                                                                                                >  1:596650/1‑300 (MQ=255)                                                                                                     aTGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTgg                                                                                                                                                                                             >  1:506040/1‑300 (MQ=255)                                                                                                                  aaCCACGGTTCAGCGTCGGCGTCATCGACATCGACGATGCCGTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAAt                                                                                                                                                                                <  2:16435/300‑1 (MQ=255)                                                                                                                    ccACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTGATGTGTCGCCGCAACCGCCATCGACTGNTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATag                                                                                                                                                                              >  2:300134/1‑300 (MQ=255)                                                                                                                         gTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCAc                                                                                                                                                                         <  1:457523/300‑1 (MQ=255)                                                                                                                                   ggCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAagag                                                                                                                                                               <  1:477177/300‑1 (MQ=255)                                                                                                                                      gTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTac                                                                                                                                                            <  1:524641/300‑1 (MQ=255)                                                                                                                                          tCGACATCAACGGTGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTacacac                                                                                                                                                        <  2:48543/300‑1 (MQ=255)                                                                                                                                           cGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACAcc                                                                                                                                                       <  1:437630/300‑1 (MQ=255)                                                                                                                                            gACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCg                                                                                                                                                      >  1:71958/1‑300 (MQ=255)                                                                                                                                             aCATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGACAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGAATGCTCCGGATACGCTTTCGGTGCATGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCgc                                                                                                                                                     >  2:317128/1‑300 (MQ=255)                                                                                                                                                tCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCgctgc                                                                                                                                                  <  2:400543/300‑1 (MQ=255)                                                                                                                                                        gCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGc                                                                                                                                          >  1:173722/1‑300 (MQ=255)                                                                                                                                                               gCCCTGCACGTTTCGGCAGACAACCATATTTATGCAGTGCAGCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGa                                                                                                                                   <  2:566708/300‑1 (MQ=255)                                                                                                                                                               gCCCGGCACGTTTCGTCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTNCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCATGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTNGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGa                                                                                                                                   <  2:383264/300‑1 (MQ=255)                                                                                                                                                                         tttCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCATGNTTGTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTTTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTAc                                                                                                                         <  1:295428/300‑1 (MQ=255)                                                                                                                                                                                         tatTTATGCAGTGCATCCAGCAGCCCTTGCTTTGCCAGTTCGCGATCCTGATATAGACCGCGCATTACCACTTCATCCGGATACAACCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGTATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCCCTACCGGTACGGTACCgaga                                                                                                         <  2:31472/300‑1 (MQ=255)                                                                                                                                                                                                 cAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCTAGATCTTCAcc                                                                                                 <  1:505599/300‑1 (MQ=255)                                                                                                                                                                                                   gTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCaa                                                                                               >  1:271455/1‑300 (MQ=255)                                                                                                                                                                                                       aTCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCTTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCAc                                                                                           >  1:214048/1‑300 (MQ=255)                                                                                                                                                                                                            gcagcCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCTTTAGACCCCCGCACTCCCATTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATAc                                                                                      <  1:16376/300‑1 (MQ=255)                                                                                                                                                                                                            catcccctgcgagatgtgtataagatacaagATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGTTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGGACGGTACCGAGATCTTCACCAATCACCATAc                                                                                      <  2:495247/270‑1 (MQ=255)                                                                                                                                                                                                                              gTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCCGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGAAGTTTACttt                                                                    >  2:235037/1‑300 (MQ=255)                                                                                                                                                                                                                                      tCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGCTGACGTTTACTTTCGAGTGcc                                                            >  2:234400/1‑300 (MQ=255)                                                                                                                                                                                                                                      tCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGcc                                                            >  1:310107/1‑300 (MQ=255)                                                                                                                                                                                                                                       ccTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGNGGTCGTTTTCGACATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTAACGAAAATCTCTAACGGTACGGTACCGAGATTTTCACCAATCATCACACAGTGACGACGTTCACTTTCGAGTACCa                                                           >  2:89647/1‑300 (MQ=255)                                                                                                                                                                                                                                          gATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGANTGCTCCGGATACGCTTTCGGTGCACGNAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCAGTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGACCTTCACCAATCACCATACAGCGCTGACGTTTACTTTCGAGTGCCAGaa                                                        >  2:616989/1‑300 (MQ=255)                                                                                                                                                                                                                                          gATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGTCAGaa                                                        >  1:510888/1‑300 (MQ=255)                                                                                                                                                                                                                                          gATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGaa                                                        >  2:180815/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gcgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAg                                              >  1:463367/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gcgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTAACCAATCACTATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAg                                              >  2:192267/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCTCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGatcat                                         >  1:45209/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGatcat                                         >  1:161119/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTAACGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGatcat                                         >  2:178793/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    tCCGGAGACAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCTAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAg                              <  1:164750/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            cAGCCCCAGGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGNTCGTTTTCGAAATAGAGCACTTTTTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATa                      <  2:88381/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                cccATGGTTTTGCCCAGCGTTAGACCCCCGCACTCCCACTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATAcgcg                  <  2:477646/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    gggTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTATACCGGTACGGTACCGAGATCTTCACCGATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCt              >  2:687239/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gTTTTGCCCAGCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGa            >  1:330254/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                gCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGGACGTCTTCTCGTGGTCGTTTTCGAANTAGAGCACTTTGTAGGAGTACACACCNCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGtct  >  2:621748/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                gCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGtct  >  1:625891/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                gCGTTAGACCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTAACGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGtct  >  1:700468/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                    GTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCT  >  NC_000913/3548097‑3548683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑