Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,224,169	A→G	F234F (TTT→TTC)	pbpG ←	peptidoglycan DD‑endopeptidase PbpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,224,169	0	A	G	100.0%	366.1 / NA	97	F234F (TTT→TTC)	pbpG	peptidoglycan DD‑endopeptidase PbpG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (52/45);  total (52/45)

GGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCG  >  NC_000913/2223875‑2224460                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                    ggAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGcc                                                                                                                                                                                                                                                                                                <  1:138212/300‑1 (MQ=255)      aaCTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAANGCTGCTGCCGGCACAGGCATCAATTTNCCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGttt                                                                                                                                                                                                                                                                                           >  2:282235/1‑300 (MQ=255)          ggCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGt                                                                                                                                                                                                                                                                                       <  2:28861/300‑1 (MQ=255)          ggCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGNATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGt                                                                                                                                                                                                                                                                                       >  2:371225/1‑300 (MQ=255)                     gAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAACACCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAatat                                                                                                                                                                                                                                                                            <  2:440697/300‑1 (MQ=255)                          aaaaTTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAg                                                                                                                                                                                                                                                                       <  1:743035/300‑1 (MQ=255)                            aaTTACNGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTNTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGtt                                                                                                                                                                                                                                                                     <  2:347415/300‑1 (MQ=255)                                  ggATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCAGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCNTCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCAAGTTATcgcg                                                                                                                                                                                                                                                               >  1:644835/1‑300 (MQ=255)                                      ggCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCTGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTAAGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATac                                                                                                                                                                                                                                                           <  1:392197/300‑1 (MQ=255)                                      ggCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATac                                                                                                                                                                                                                                                           <  1:656134/300‑1 (MQ=255)                                       gCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATaca                                                                                                                                                                                                                                                          >  1:527984/1‑300 (MQ=255)                                          gagTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCa                                                                                                                                                                                                                                                       >  1:182459/1‑300 (MQ=255)                                            gTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCACGCCGCATTGGTGAAGACGGTTTTGGTTAACTGAACATCCCAGTTATCGCGATACACCaaa                                                                                                                                                                                                                                                     >  2:523619/1‑298 (MQ=255)                                             tATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAgat                                                                                                                                                                                                                                                    <  2:659636/300‑1 (MQ=255)                                                 gCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGAtt                                                                                                                                                                                                                                                <  1:613880/300‑1 (MQ=255)                                                    aTCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGANGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATNATTAGt                                                                                                                                                                                                                                             >  2:678330/1‑300 (MQ=255)                                                      ccGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTAt                                                                                                                                                                                                                                           <  2:181090/300‑1 (MQ=255)                                                      ccGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCGGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTAt                                                                                                                                                                                                                                           <  2:213743/300‑1 (MQ=255)                                                        gAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGTATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTAACAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGACCGCCGCACTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACCACAGATGATTAGTATTg                                                                                                                                                                                                                                         >  2:117248/1‑300 (MQ=255)                                                        gAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAACATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCACCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCAGCATTGGTGGAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTAttt                                                                                                                                                                                                                                         >  2:603621/1‑299 (MQ=255)                                                                      gTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCATAGGCGTCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATCTTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCACGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCAAGTTATCGCGATACAACAGATGAGTAGTATTGCGGAAAGGCAGCGt                                                                                                                                                                                                                           >  2:139739/1‑300 (MQ=255)                                                                      gTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGt                                                                                                                                                                                                                           >  1:103908/1‑300 (MQ=255)                                                                             gCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCACCGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCACTGGTGAAGCCGGTTTTGGTTAACTGAATATTCAAGTTATCGCGATAAACAAGATGGTTAGTATTGCGGAACGGCAGTGTATActtc                                                                                                                                                                                                                    >  2:440784/1‑297 (MQ=255)                                                                                gTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTNTTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGTTTAACTGCATATTCCAGTTATCGCGGTACACCAGATGATTAGTATTGCGGAACGGAGGCGTATACGTCGGa                                                                                                                                                                                                                 >  2:484935/1‑300 (MQ=255)                                                                                    gCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTag                                                                                                                                                                                                             >  1:505214/1‑300 (MQ=255)                                                                                       ccgccgccgCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTGTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGTTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAgaga                                                                                                                                                                                                          <  2:424289/300‑1 (MQ=255)                                                                                            gccgccATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGtt                                                                                                                                                                                                     <  1:230708/300‑1 (MQ=255)                                                                                                   tttGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCatat                                                                                                                                                                                              <  1:78783/300‑1 (MQ=255)                                                                                                            cTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCggg                                                                                                                                                                                     <  2:585963/300‑1 (MQ=255)                                                                                                                  gTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTAc                                                                                                                                                                               <  1:135943/300‑1 (MQ=255)                                                                                                                       ttagaGCTCAACGTTGCTTCCGGCACAGGCAGCGCTTTACCGGTTTCAATCCAGGTACGCAGGCGTCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGTTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGTTAGTACTTAAc                                                                                                                                                                          <  2:50075/296‑1 (MQ=255)                                                                                                                       ttATAGCTCAACGCTGCTGCCTGCACATGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAAc                                                                                                                                                                          <  2:50158/300‑1 (MQ=255)                                                                                                                       ttATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCTGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAAc                                                                                                                                                                          <  2:67133/300‑1 (MQ=255)                                                                                                                       ttATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCNTCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAAc                                                                                                                                                                          <  2:694769/300‑1 (MQ=255)                                                                                                                       ttATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAAc                                                                                                                                                                          <  2:101129/300‑1 (MQ=255)                                                                                                                       ttATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAAc                                                                                                                                                                          <  1:363435/300‑1 (MQ=255)                                                                                                                            gCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCNGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGccc                                                                                                                                                                     <  1:301317/300‑1 (MQ=255)                                                                                                                            gCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGccc                                                                                                                                                                     >  1:18698/1‑300 (MQ=255)                                                                                                                                    ctgctgCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATNACCGTACGCATCACCAGACAATGGCCNGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACg                                                                                                                                                             >  2:284564/1‑300 (MQ=255)                                                                                                                                      gctgcCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGa                                                                                                                                                           >  1:71617/1‑300 (MQ=255)                                                                                                                                        tgcCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGata                                                                                                                                                         >  1:728345/1‑300 (MQ=255)                                                                                                                                                 aGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCt                                                                                                                                                >  1:610124/1‑300 (MQ=255)                                                                                                                                                  ggCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTg                                                                                                                                               >  1:485204/1‑300 (MQ=255)                                                                                                                                                      tCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTCCCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCAGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCaa                                                                                                                                           >  2:35274/1‑300 (MQ=255)                                                                                                                                                        aCTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATg                                                                                                                                         >  1:444231/1‑300 (MQ=255)                                                                                                                                                         cTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTCTTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGa                                                                                                                                        >  1:479346/1‑300 (MQ=255)                                                                                                                                                         cTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCAGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGa                                                                                                                                        >  2:257994/1‑300 (MQ=255)                                                                                                                                                           ttACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCTGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGc                                                                                                                                      <  1:609829/300‑1 (MQ=255)                                                                                                                                                                 gTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTg                                                                                                                                >  1:174947/1‑300 (MQ=255)                                                                                                                                                                     cAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGGCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCANCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTa                                                                                                                            <  2:361832/300‑1 (MQ=255)                                                                                                                                                                           aGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCAc                                                                                                                      >  1:247073/1‑300 (MQ=255)                                                                                                                                                                              tACGCAGTCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACggg                                                                                                                   <  2:538506/300‑1 (MQ=255)                                                                                                                                                                              tACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACggg                                                                                                                   <  1:20486/300‑1 (MQ=255)                                                                                                                                                                               aCGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGc                                                                                                                  <  1:693856/300‑1 (MQ=255)                                                                                                                                                                                 gCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTGTATTATGGATAACCATACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGNTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAg                                                                                                                <  2:481547/300‑1 (MQ=255)                                                                                                                                                                                         cTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACg                                                                                                        <  2:699377/300‑1 (MQ=255)                                                                                                                                                                                         cTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTGAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGANCGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGANCAGTTTGGTTAAGTCACGGGCAGTTGAAACg                                                                                                        <  2:295099/300‑1 (MQ=255)                                                                                                                                                                                                cGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCa                                                                                                 >  1:179275/1‑300 (MQ=255)                                                                                                                                                                                                 ggCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCAc                                                                                                <  1:535877/300‑1 (MQ=255)                                                                                                                                                                                                 ggCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCACCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCANTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCAc                                                                                                <  2:489071/300‑1 (MQ=255)                                                                                                                                                                                                     aaaTGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGAc                                                                                            <  1:655803/300‑1 (MQ=255)                                                                                                                                                                                                         gggTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATc                                                                                        >  1:371909/1‑300 (MQ=255)                                                                                                                                                                                                               atTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAg                                                                                  <  1:424438/300‑1 (MQ=255)                                                                                                                                                                                                                 gtgtcAAACGCGGCCATCACTACCAGCGCCACCGGTTTATGATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGTTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGGTTAGTATTGCGGAACGGCAGCGTATACGTCGGATTATAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGt                                                                                <  2:432710/296‑1 (MQ=255)                                                                                                                                                                                                                        aCGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGNAGCGTATACGTCGGATTAGAGAAGGTNGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTcaacaa                                                                         <  2:280088/300‑1 (MQ=255)                                                                                                                                                                                                                        aCGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTcaacaa                                                                         <  2:724348/300‑1 (MQ=255)                                                                                                                                                                                                                        aCGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTcaacaa                                                                         <  1:234658/300‑1 (MQ=255)                                                                                                                                                                                                                            gTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCATACAATGGCCCGCCGCATTGGTGAAGCCGGTTGTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAgcg                                                                     <  1:719358/300‑1 (MQ=255)                                                                                                                                                                                                                             tCCATCACTACCAGCGCCACCGGTTTATTATNGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATNATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACGAAgcgc                                                                    >  2:296179/1‑300 (MQ=255)                                                                                                                                                                                                                                aTCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCgtg                                                                 >  1:679611/1‑300 (MQ=255)                                                                                                                                                                                                                                aTCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAAAAAAACGCgtg                                                                 >  2:510696/1‑300 (MQ=255)                                                                                                                                                                                                                                aTCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCACGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCgtg                                                                 >  2:597490/1‑300 (MQ=255)                                                                                                                                                                                                                                          acgcCACCGGTTGATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGGAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTc                                                       <  2:421423/299‑1 (MQ=255)                                                                                                                                                                                                                                                   gTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGACGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATTTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGTGTGTTGTTCATTACGAGCGAtc                                              >  2:53358/1‑299 (MQ=255)                                                                                                                                                                                                                                                   gTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtt                                              >  2:655646/1‑300 (MQ=255)                                                                                                                                                                                                                                                     ttattaTTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCAGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtttc                                            >  1:480477/1‑300 (MQ=255)                                                                                                                                                                                                                                                          attGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACNTCGGATTAGAGAAGGTTGCCATATCTNCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCGGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAACGAtttcgcttt                                       >  2:273469/1‑300 (MQ=255)                                                                                                                                                                                                                                                          attGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtttcgcttt                                       >  1:469337/1‑300 (MQ=255)                                                                                                                                                                                                                                                          attGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtttcgcttt                                       >  2:583708/1‑300 (MQ=255)                                                                                                                                                                                                                                                          attGATAACCGTACACATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtttcgcttt                                       >  2:706755/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gATAACCGTACGCATCACCAGACAATGGCACGCCGCATTGGTGAAGCCGGTTTTGGTTAACCGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtgtcgctttcgc                                    >  2:65436/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 aCCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGcattc                                <  1:537006/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  ccGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGNTCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGcattca                               >  2:88627/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  ccGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGcattca                               >  1:391257/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       cGCATCACCAGACAATGGCCCGCCGCATTGATGAAGCCGGTTTTGGTTACCTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGCAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAAGTTCAACAAAGCGCGTGTTGTTCATTCCGCGCGATTTCGCTTTCGCATTCATTGcc                          >  2:522520/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         cATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCtt                        >  2:258540/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           tCACCAGACCATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATTAACGGANATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATACGGTAGGTTCAACAAAGCGCGTGTTGTTCCTTCCGAGCGATCTCGCTTTCGCCTTCATTGCCttca                      >  2:483212/1‑298 (MQ=255)                                                                                                                                                                                                                                                                           tCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTtaa                      >  1:2801/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           tCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCAGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTtaa                      >  2:540984/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           tCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCACGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTCGTTCATTCCGAGCGATTTCGCTTTCGCCTTCATTGCCTtaa                      >  2:182648/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                aGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGTCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAg                 >  1:592475/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 gACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCACCGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTACGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGTGATTTCGCTTTCGCATTCATTGCCTTAATAAAgg                >  2:577109/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  aCAATGTCCCGCCGCATTGGTGAAGCCGTTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGGCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGTTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGc               <  2:522580/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aCAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCAGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGc               >  1:165798/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          ccgccgCATTGGGGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAac       <  1:127686/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             ccgcATGGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTTAAACGTTATGCACCGACAATCCGTTAGGTTCAACAAAGCGCGTGTGGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac    <  2:525214/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               gcATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCg  >  1:716818/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                    GGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCG  >  NC_000913/2223875‑2224460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑