Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,311,535 G→A V149V (GTG→GTA)  tonB → Ton complex subunit TonB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,311,5350GA89.7% 218.7 / NA 78V149V (GTG→GTA) tonBTon complex subunit TonB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (38/32);  minor base C (8/0);  total (46/32)
Fisher's exact test for biased strand distribution p-value = 1.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCC  >  NC_000913/1311240‑1311828
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      
cGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCNTTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCCAAGCCGGTGAAAAAGGTACAGGAGCANCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCtt                                                                                                                                                                                                                                                                                                    <  2:671375/300‑1 (MQ=255)
cGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCNAAGCCGGTGAAAAAGGTACAGGAGCANCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCtt                                                                                                                                                                                                                                                                                                    <  2:572061/300‑1 (MQ=255)
cGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTTCGGCATCACCGTTTGAAAATACGGCCCCGGCACGCCTGGCA‑TCAAGTACAGCAAAGGCTGCAACCAGCAAGACGGTTGCCAGTGTAGCtt                                                                                                                                                                                                                                                                                                    >  2:630976/1‑300 (MQ=255)
cGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCtt                                                                                                                                                                                                                                                                                                    <  2:503001/300‑1 (MQ=255)
cGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGNAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACNCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCtt                                                                                                                                                                                                                                                                                                    <  2:773354/300‑1 (MQ=255)
 gCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTc                                                                                                                                                                                                                                                                                                   <  1:793427/300‑1 (MQ=255)
      ctgATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGAc                                                                                                                                                                                                                                                                                              <  2:447095/300‑1 (MQ=255)
      ctgATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGAc                                                                                                                                                                                                                                                                                              <  1:682185/300‑1 (MQ=255)
      ctgATCTCGAACCGCCACAAGCCATTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCCGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCTTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGAc                                                                                                                                                                                                                                                                                              <  2:326282/300‑1 (MQ=255)
          tctcGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCAACGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCAcg                                                                                                                                                                                                                                                                                          >  1:451410/1‑300 (MQ=255)
               aaCCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCAt                                                                                                                                                                                                                                                                                     <  1:675771/300‑1 (MQ=255)
                    ccACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCACGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCAAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCCTTACCGTTTTACACCACGGCACCGGCACGCCCTACACTCA‑GTACAGACACGGCCGCAACCAACAAGACGGTTACCATTGTCACCTCAGGACCACGTGCACTAAga                                                                                                                                                                                                                                                                                >  2:430971/1‑299 (MQ=255)
                        aaGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCNTCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTa                                                                                                                                                                                                                                                                            >  1:589659/1‑300 (MQ=255)
                           ccGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATc                                                                                                                                                                                                                                                                         <  2:788544/300‑1 (MQ=255)
                             gTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCAGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCAACGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTCGCTTCAGGACCACGCGCATTAAGCCGTAACCAg                                                                                                                                                                                                                                                                       >  1:12353/1‑300 (MQ=255)
                              ttCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGc                                                                                                                                                                                                                                                                      <  1:648097/300‑1 (MQ=255)
                                                cGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCCGCCGCAGTATCCGGCACGAg                                                                                                                                                                                                                                                    >  1:494583/1‑300 (MQ=255)
                                                    ggtAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATTACCGTTTGGAAATACAGAACCGGCACGCCTGACA‑TCCAATCCCGAAAAGGCTGCAAACAGTAAGCCGGTTACAAGTGTAGCTTCAGGACCAAGCGACTTAAGCCGTAATTAGTCGCAATATCCggaacaagccaa                                                                                                                                                                                                                                                >  2:228695/1‑290 (MQ=255)
                                                              gAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTNAAAAGCCGAAGCCGAAACCTAAGCCAAGACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTgcgc                                                                                                                                                                                                                                      <  2:618785/300‑1 (MQ=255)
                                                              gAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCCAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTgcgc                                                                                                                                                                                                                                      >  1:712742/1‑300 (MQ=255)
                                                              gAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCAACGTTTGAAAATACGGCACCGGCACGCCTGACC‑TTAAGTCCAGCAACGGCTGCAACCAACAAGCAGGTTACCCGTGTAGATTCAGGACCAAGCGCATTCAGACGTCATAAGCCGCAGTATCTGGCACGAGTAAAGGCATTgggc                                                                                                                                                                                                                                      >  2:415306/1‑297 (MQ=255)
                                                                aCCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCACACCCGAAGCAGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCAGCACCACCATTTTAAAATCCTGCACCAGCCCACCTGACA‑TCAAGTACCGCAACGGATGCAACTAGCAAGCAGGTTGACAGTGGAGCTTCAGGACCAANAGGATTAAAGAGTAATCAGATGCAGTATTCGGGAATAGCACAGGCATTGCGCAt                                                                                                                                                                                                                                    >  2:618273/1‑300 (MQ=255)
                                                                        cTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGc                                                                                                                                                                                                                            <  2:421371/300‑1 (MQ=255)
                                                                                 tCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGACGGTTACCAGTGTAGCTTCAGGACCACGCGCATTGAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGgttaaag                                                                                                                                                                                                                   >  1:768321/1‑300 (MQ=255)
                                                                                         ccGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCAGCATCCCCGTTTGAAAATACTGCCCCGGCACGCCTTACA‑TCCAGTCCCACCACTGCTTCCACCAGCAAGCCGGTTCCCAGTGTCGCTTCAGGACCAAGCGCCTTCAGCCGTAATCCGCCGCCGTCCTCGGCACGAGCACAGGCCTTGCGCATTGAAGGGGAGgttaaagttcacgtt                                                                                                                                                                                                           >  2:814996/1‑294 (MQ=255)
                                                                                          cGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCNTCCCCGTTTTAAAATACGGCACCGGCNCGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCCGTGTAGCTTCAGGACCACGCGCATTCAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGAAGGTTAAAGTTCAATTTg                                                                                                                                                                                                          >  2:561175/1‑300 (MQ=255)
                                                                                           gCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGa                                                                                                                                                                                                         >  1:803027/1‑300 (MQ=255)
                                                                                           gCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCAAGAGCATTAAGACGTAATCAGCCGCAGTGTTCGGCACGAGCACAGGCGTTGAGAATTGAAGGGAAGGTTAAAGTTAAATTTGa                                                                                                                                                                                                         >  2:783661/1‑300 (MQ=255)
                                                                                              aaaaGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCNAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTANCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGt                                                                                                                                                                                                      <  2:766145/300‑1 (MQ=255)
                                                                                                aagaagCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTCAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTa                                                                                                                                                                                                    >  1:721874/1‑300 (MQ=255)
                                                                                                     gCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCCCGCCTGACA‑TCCAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTCCCCGTGTAGCTTCAGGACCCCTCGCATTCCGCCGTCACCAACCGTAGTCTCCTGCACGAGCACCGGCCTTTCGCATTGAAGGGCCGGTTCAAGTTAAACTTGAAGttcagcct                                                                                                                                                                                               >  2:633970/1‑294 (MQ=255)
                                                                                                      cACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCAGGTGGAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTCGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGTTCATTTAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCgg                                                                                                                                                                                             >  1:250258/1‑300 (MQ=255)
                                                                                                                cATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCCCCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGt                                                                                                                                                                                   >  1:749938/1‑300 (MQ=255)
                                                                                                                              agccgaaACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACaaa                                                                                                                                                                     <  2:705928/300‑1 (MQ=255)
                                                                                                                               gccgaaACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCTGCATCACCGTTTGACAACACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCCAGCCGGTTCCCAGTGTAGCCTCAGGACCACGCGCCTTACGCCTTAACCAGCCGCAATATCCGGCACAAGCACAAGCACTGCGCATTGAAAGGGAGGTTAAAATTAAATTTGACGTTCAG‑ACAGACGGTCGCGTGGATAACGTACaaac                                                                                                                                                                    >  2:452814/1‑299 (MQ=255)
                                                                                                                                                              aaaaaGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAg                                                                                                                                     <  1:651657/300‑1 (MQ=255)
                                                                                                                                                                aaaGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCg                                                                                                                                   <  2:804943/300‑1 (MQ=255)
                                                                                                                                                                  aGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTCGAGTCGCGTCCGGCATCCCCATTTGAAAATACGGCCCCGGTACGCCTGACA‑TCAAGTACAGCAACAGCTGCCACCAACAAGCCGGTTACCAGTGTCGCCTCAGGACCCCACGCATTAAGCCGTAATCCGCCGCAGTCTCCGGCACGAGCACAGGCATTGTGCATTGAAGGGCAGGTTAAAGTTCCATTTTACGTTACG‑CCAGCTGGTCGCGTGGATAACGTACAAATTCTCTCCGCCAAGCCTGCGAACATGTTTGAGCGTg                                                                                                                                 >  1:151475/1‑300 (MQ=255)
                                                                                                                                                                  aGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGACAATCCTGCCCCGGCCCGCCTGCCA‑TCAAGTACAGCAACGGCTGCAACCAGCCAGCCGGTTACCAGTGTCGCTTCAGGACCACGCGCCTTGCGTCGTCATTCGNCGCAATCTTCGGCACGGGCACAGGCATTGCGCATTGAAGGGCAGGTTCAAGTTCAATTTGACATTACAACCGG‑GGGTCGCGGGGATCAAGTACAAATCCTCTCaaacaagaccctggacacgtttgagcgtg                                                                                                                                 >  2:191562/1‑272 (MQ=255)
                                                                                                                                                                  aGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATCCGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTCCCAGTGTAGCTTCAGGACCCCGCGCATTAAGACGTAATCAGCCGCCGTATCCGGCACGAGCCCAGGCATTGCGCATTGAAGGGCAGGTTCAAGTTCAATTTGACGTTACG‑CCGGATGGTCGCGTGGATCACGTCCAAATCCTCTCAGACAAGCCCGCGAACATGTTTGAGCGTg                                                                                                                                 >  2:697499/1‑300 (MQ=255)
                                                                                                                                                                    gTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCCTCACCGTTTGGAAATCCGGCACCGGCCCGCCTGACA‑TCAAGTACCGCAACAGCTGCAACCAGCAAGCCGGTTGCCAGTGTAGCTTCAGGACCACGCGCATTCAGCCGTAATCCGCCGCAGTATCCGGCACAAGCACAGGTATTGCGCACTGAAGGGCAGGTTCAAGTTAAACTTGACATTACG‑CAGGATGGTCGCGTGGATAACGTACAAACCCTCCCCGCCAAGCCTGCGAACACGTTTGAGCGGGAg                                                                                                                               >  2:282366/1‑300 (MQ=255)
                                                                                                                                                                    gTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAATACAGCAACAGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGAACACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGTATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAg                                                                                                                               >  1:126575/1‑300 (MQ=255)
                                                                                                                                                                         ggAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCCAATCCAGCAACGGCTGCAACCAGCAAGCCGGTTCCCAATGTCGCTTCCGGAACACGCGCACTAAGCCGTAACCAGCCGCAGTATCCGGCACAAACACAGGCATTGCGCAGTGAAGGGCAGGTTGAAGTTCAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACCAATCCTCTCAGCCAAGCATGAGAACATGTTTGAGCGTGAGGTGaa                                                                                                                          >  1:64320/1‑300 (MQ=255)
                                                                                                                                                                          gAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCCCCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TTAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTTCGCATTGAAGGGCAGGTTCAAGTTCAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTCCAAATCCTCTCAGCCCAGCCTGCGAACATGTTTGAGCGTGAGGTGaaa                                                                                                                         >  1:637079/1‑300 (MQ=255)
                                                                                                                                                                                     aCGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATCCGGCACCGGCCCGCCTGACA‑CCCAGTACAGCAACGGCTTCAACCAGCCAACCGGTTCACAGTGTAGATTCAGGANCACGCGCATTCAGCCATAACCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGANGGGCAGGTTAAAGTTAAATTTGACGTTACG‑ACGGATGGTCGCGTGGATAACATACAAATCCTCTGCGCCAAGCCTGTGAACATGTTTGAGCGTGAGGTGAAAAatgcgatgcg                                                                                                              >  2:652859/1‑300 (MQ=255)
                                                                                                                                                                                           tggcAAACCCGTAGAGTCGCGTCCGGCATCACCGGTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACAGCTGCAACCAGCAAGCCGGGTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATg                                                                                                        <  2:449041/297‑1 (MQ=255)
                                                                                                                                                                                            gTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGGGGTGAAAAATGCGATGCGCAGATgg                                                                                                       >  1:694710/1‑300 (MQ=255)
                                                                                                                                                                                              cAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGNAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCg                                                                                                     <  2:201349/300‑1 (MQ=255)
                                                                                                                                                                                                aaCCCGTAGAGTCGCGTCCGGCATCCCCGTTNGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTCGCTTCAGGACCCCGCGCCTTNAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGAATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAACCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGtt                                                                                                   >  2:588068/1‑300 (MQ=255)
                                                                                                                                                                                                aaCCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAACAACGGCTGCAACCAGCAAGCCGGTTACCCGCGTAACTTCAGGACCACGCGCATTNAGCCGTAATCAGCCGCAGTATCCGGCNCGAGCACAGGCATTTCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTCCG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCATGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGAGtt                                                                                                   >  2:559265/1‑300 (MQ=255)
                                                                                                                                                                                                    cttAGAGTCGCGTCCGGCATCACCGGTTGAAAATACGGCACCGGCACGCCTTACA‑TCAAGTACAGCAACGGCTGCAACCAGCTAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGa                                                                                               <  2:497655/298‑1 (MQ=255)
                                                                                                                                                                                                      tAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACTGGCACGCCTGACA‑TCAAGTACAGTAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCGCGAGCACATGCATTGCGCATTGAATGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGc                                                                                             <  2:396820/300‑1 (MQ=255)
                                                                                                                                                                                                          gTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGTGTGAGGTGAACAATGCGATGCGCAGATGGCGTTATGAGCCggg                                                                                         >  2:364666/1‑300 (MQ=255)
                                                                                                                                                                                                                tCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCGGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTCAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGcc                                                                                   <  2:502601/300‑1 (MQ=255)
                                                                                                                                                                                                                tCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGcc                                                                                   <  1:15303/300‑1 (MQ=255)
                                                                                                                                                                                                                  cGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCCGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTNAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTAAGCAAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCCGATGGCGTTATGAGCCGGGTAAGcaag                                                                                 >  2:185459/1‑297 (MQ=255)
                                                                                                                                                                                                                    gCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGCAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGc                                                                               <  2:504243/300‑1 (MQ=255)
                                                                                                                                                                                                                      aTCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTTAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAg                                                                             <  2:408869/300‑1 (MQ=255)
                                                                                                                                                                                                                                      aCGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTTAAGGGCAGGTTATAGTTAAATTTGACGTTACG‑CCGGATGNTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCNGGGTAAGCCAGGCAGTGGGATTGTGGTGAat                                                             <  2:577081/300‑1 (MQ=255)
                                                                                                                                                                                                                                          caccggcacGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTTTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTTAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATcc                                                         <  2:322181/300‑1 (MQ=255)
                                                                                                                                                                                                                                          caccggcacGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCNGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTCAAATTTGACGTTACG‑CCGGATGGGCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATcc                                                         <  2:719949/300‑1 (MQ=255)
                                                                                                                                                                                                                                          caccggcacGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATcc                                                         <  2:334732/300‑1 (MQ=255)
                                                                                                                                                                                                                                          caccggcacGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAACTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGTTTAAAGTTAAATTTGACGCTACG‑CCGGATGGTCGCGTGGATAACGCACAAATCCTCTCAGCCAAGCCTGCGAACAGGTTTGAGCGTTAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAataccc                                                         <  1:438798/300‑4 (MQ=255)
                                                                                                                                                                                                                                                        aCA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAAc                                           >  2:223654/1‑300 (MQ=255)
                                                                                                                                                                                                                                                          a‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGACCATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACgg                                         >  2:119116/1‑300 (MQ=255)
                                                                                                                                                                                                                                                              aaGTACAGCAACGGCTGCAACCAGCAAGCCGNTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTNAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCAGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGcac                                      >  2:596396/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                gTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGcacca                                    >  1:473234/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                    aGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTAAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCAAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGa                                >  2:748531/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                         cggcggCAACCAACAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGTATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGAATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGTTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGTTGAACATCCTGTTTaaggataaggcgaccaccgagattc                           <  2:145364/300‑24 (MQ=255)
                                                                                                                                                                                                                                                                         cGGCTGCAACCAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCTGCACGAGCACAGGCATTGCGCATTGAAGGGCAGTTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGTGATTTTGGTGAATATCCTGTTTaagggtaatggtaccaccgagattc                           <  2:703248/300‑24 (MQ=255)
                                                                                                                                                                                                                                                                                   cAGCAAGCCGGTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGCGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGa                 >  2:290763/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                            ggTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCaaaa        >  1:158/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                            ggTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCATCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCAGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCaaaa        >  1:167/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                            ggTTACCAGTGTAGCTTAAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCCGTGGGATTGTGGTGAATATCCTGTTTAAAATTCACGGCACCACCGAAATTCAGTAAACAGAAAGTCagaa        >  2:379328/1‑297 (MQ=255)
                                                                                                                                                                                                                                                                                             gTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAg       >  1:333927/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                             gTTACCAGTGTAGCTTCAGGACCACGCGCATTAAGCAGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTAGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGCGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAACTCAGTAAGCAGACAGTCaaagg       >  2:169000/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                               tACCAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGca     >  2:538314/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                                  cAGTGTAGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTcc  >  2:525502/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      
CGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACA‑TCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACG‑CCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCC  >  NC_000913/1311240‑1311828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑