Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,157,998	G→A	E383E (GAG→GAA)	argH →	argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,157,998	0	G	A	98.9%	314.2 / ‑6.9	91	E383E (GAG→GAA)	argH	argininosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (61/29);  minor base C (0/1);  total (61/30)
Fisher's exact test for biased strand distribution p-value = 3.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGGAGCTGATTCGCGGTAAATGCGGCCGGGTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTATATGTATAAATTTGAGCCTGGCTTATCGCCGGGCTTTTTTATGGCAAAAAAAAGCG  >  NC_000913/4157712‑4158288                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                   cgcTGGAGCTGATTCGCGGTAAATGCGGCCGGGTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAANGTCCACGTTGCCAGGCAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCCCCATAttg                                                                                                                                                                                                                                                                                       >  2:727875/1‑300 (MQ=255)  gcTGGAGCTGATTCGCGGTAAATGCGGCCGGGTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATAttgt                                                                                                                                                                                                                                                                                      <  1:46540/300‑1 (MQ=255)    tGGAGCTGATTCGCGGTAAATGCGGCCGGGTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATAttgttg                                                                                                                                                                                                                                                                                    <  1:647949/300‑1 (MQ=255)    tGGAGCTGATTCGCGGTAAATACGGCCGGGTGCATGGGGCGTTAACCGGCATGATGATGACACGGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGTACTGTCTGCATATGGCGGCGCTGGTACTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATAttgttg                                                                                                                                                                                                                                                                                    <  2:325570/300‑1 (MQ=255)      gAGCTGATTCGCGGTAAATGCGGCCGGGTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGNAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGt                                                                                                                                                                                                                                                                                  <  1:582532/300‑1 (MQ=255)      gAGCTGATTCGCGGTAAATGCGGCCGGGTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGt                                                                                                                                                                                                                                                                                  <  1:395783/300‑1 (MQ=255)          tGATTCGCGGTAAATGCGGCCGGGTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAg                                                                                                                                                                                                                                                                              <  1:480900/300‑1 (MQ=255)             ttCGCGGTAAATGCGGCCGGGTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCgg                                                                                                                                                                                                                                                                           <  1:449100/300‑1 (MQ=255)                            gCCGGGTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAANGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGACCTGGCGAATTATCTGGTGGCGAAAGGCGTACCGTTCAGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTc                                                                                                                                                                                                                                                            >  2:206885/1‑300 (MQ=255)                              cGGGTGCAGGAAGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCACTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGTGTCCCGTTCCGCGAAGCGCACTATATTGTTGGTGAAGTGGTGGTGGCAGCCAtttgt                                                                                                                                                                                                                                                          >  2:52492/1‑297 (MQ=255)                                 gTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAg                                                                                                                                                                                                                                                       >  2:404258/1‑300 (MQ=255)                                 gTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAg                                                                                                                                                                                                                                                       >  1:56172/1‑300 (MQ=255)                                    cAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGTTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGc                                                                                                                                                                                                                                                    <  1:702398/300‑1 (MQ=255)                                    cAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGc                                                                                                                                                                                                                                                    <  1:76079/300‑1 (MQ=255)                                         ggCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAAcc                                                                                                                                                                                                                                               <  2:638567/300‑1 (MQ=255)                                          gCGTTAACCGGCATGATGATGACGCTGAAAGNTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGNGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCg                                                                                                                                                                                                                                              >  2:582370/1‑300 (MQ=255)                                          gCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAANGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCg                                                                                                                                                                                                                                              >  2:720508/1‑300 (MQ=255)                                          gCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCg                                                                                                                                                                                                                                              >  1:327052/1‑300 (MQ=255)                                                        gatgatgaCGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGcc                                                                                                                                                                                                                                >  1:397362/1‑300 (MQ=255)                                                        gatgatgaCGCTGAAAGGTTTGCCGCTGGCTNACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACNGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGGTCTggc                                                                                                                                                                                                                                >  2:598708/1‑298 (MQ=255)                                                              gaCGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAg                                                                                                                                                                                                                          <  1:538906/300‑1 (MQ=255)                                                                 gCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGa                                                                                                                                                                                                                       >  2:791734/1‑300 (MQ=255)                                                                 gCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGa                                                                                                                                                                                                                       >  1:619815/1‑300 (MQ=255)                                                                 gCTGAAAGGTTTGCCGCTGGCTTACAAAAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGa                                                                                                                                                                                                                       >  1:619822/1‑300 (MQ=255)                                                                    gAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGtt                                                                                                                                                                                                                    >  1:335901/1‑300 (MQ=255)                                                                                              aaGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACg                                                                                                                                                                                          >  1:18202/1‑300 (MQ=255)                                                                                                gATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGaa                                                                                                                                                                                        >  1:347655/1‑300 (MQ=255)                                                                                                    tGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGTACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATg                                                                                                                                                                                    <  2:334856/300‑1 (MQ=255)                                                                                                      cAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTc                                                                                                                                                                                  <  1:423097/300‑1 (MQ=255)                                                                                                           aGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATcc                                                                                                                                                                             >  2:432896/1‑300 (MQ=255)                                                                                                            gACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGNTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCg                                                                                                                                                                            <  1:593465/300‑1 (MQ=255)                                                                                                                    agGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTc                                                                                                                                                                    >  2:426059/1‑300 (MQ=255)                                                                                                                     ggTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGCCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCg                                                                                                                                                                   >  2:709316/1‑300 (MQ=255)                                                                                                                        cTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTCAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTg                                                                                                                                                                <  2:522100/300‑1 (MQ=255)                                                                                                                                    cTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCCGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTc                                                                                                                                                    >  2:442188/1‑300 (MQ=255)                                                                                                                                    cTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTc                                                                                                                                                    >  1:353659/1‑300 (MQ=255)                                                                                                                                    cTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTAAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAACCCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGAAGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGcata                                                                                                                                                    >  2:402778/1‑297 (MQ=255)                                                                                                                                       gATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGAc                                                                                                                                                 <  1:156619/300‑1 (MQ=255)                                                                                                                                        aTACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACa                                                                                                                                                >  1:91830/1‑300 (MQ=255)                                                                                                                                          aCCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAg                                                                                                                                              >  1:118747/1‑300 (MQ=255)                                                                                                                                            ctggctggACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAANCGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCg                                                                                                                                            <  2:615021/300‑1 (MQ=255)                                                                                                                                            ctggctggACTACCTGCATATGGCGTCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCg                                                                                                                                            <  2:486242/300‑1 (MQ=255)                                                                                                                                             tggctggACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCNGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGt                                                                                                                                           >  1:598389/1‑300 (MQ=255)                                                                                                                                             tggctggACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGt                                                                                                                                           >  1:12105/1‑300 (MQ=255)                                                                                                                                             tggctggACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGt                                                                                                                                           >  2:40704/1‑300 (MQ=255)                                                                                                                                                       gcctgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCNAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAANATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAg                                                                                                                                 >  2:778049/1‑300 (MQ=255)                                                                                                                                                       gcctgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTNCCGTTCCGCGAAGCGCACCATATTGTNGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAg                                                                                                                                 >  2:571562/1‑300 (MQ=255)                                                                                                                                                         ctgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAggc                                                                                                                               <  2:702010/300‑1 (MQ=255)                                                                                                                                                         ctgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAggc                                                                                                                               <  1:394095/300‑1 (MQ=255)                                                                                                                                                           gcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAggcgg                                                                                                                             >  1:380239/1‑300 (MQ=255)                                                                                                                                                                tGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCAACGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTAGACAAGCGTGCGGCAAAAGGCGGCGtct                                                                                                                        >  2:18915/1‑300 (MQ=255)                                                                                                                                                                     gcgcTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTAACCg                                                                                                                   >  1:548442/1‑300 (MQ=255)                                                                                                                                                                           gTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGAAGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGcagcag                                                                                                             >  2:282855/1‑300 (MQ=255)                                                                                                                                                                           gTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGAGTGACGAAGATGTCTATCCGATTCTGTCNCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGcagcag                                                                                                             >  2:627787/1‑300 (MQ=255)                                                                                                                                                                           gTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCAACGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTAAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGcagcag                                                                                                             >  1:164776/1‑300 (MQ=255)                                                                                                                                                                                ggACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGgc                                                                                                        <  1:447649/300‑1 (MQ=255)                                                                                                                                                                                       aTTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCg                                                                                                 <  2:498137/300‑1 (MQ=255)                                                                                                                                                                                          cAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCAACGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGAAAGCCATTCGTCAAGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATAGTGCCTAGACAAGCGTGTGGCAGAAGGCGGCGTCTCACCGAAGCAGGTGGCGCAGGCGAtt                                                                                              >  2:52684/1‑300 (MQ=255)                                                                                                                                                                                                   cGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTgcg                                                                                     >  1:741363/1‑300 (MQ=255)                                                                                                                                                                                                    gTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTTGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTgcgc                                                                                    <  1:108721/300‑1 (MQ=255)                                                                                                                                                                                                             gCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGt                                                                           >  2:482302/1‑300 (MQ=255)                                                                                                                                                                                                              ccAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGTTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGNTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGtt                                                                          <  1:558112/300‑1 (MQ=255)                                                                                                                                                                                                              ccAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGtt                                                                          <  2:223776/300‑1 (MQ=255)                                                                                                                                                                                                                  gAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAggg                                                                      <  1:116566/300‑1 (MQ=255)                                                                                                                                                                                                                  gAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAggg                                                                      <  2:816331/300‑1 (MQ=255)                                                                                                                                                                                                                        gCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGaa                                                                >  1:425254/1‑300 (MQ=255)                                                                                                                                                                                                                           cagcagGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGCAAGGCGTACCGTTCCGCGAAGCGCAACATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTAGACAAGCGTGCGGCAAAAGGCGGCGTCTAACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAAcct                                                             >  1:695241/1‑298 (MQ=255)                                                                                                                                                                                                                           cagcagGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCCGAAATTCAGTAAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCCAGAGGTGGAGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACAt                                                             >  2:322471/1‑300 (MQ=255)                                                                                                                                                                                                                           cagcagGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACAt                                                             >  1:762428/1‑300 (MQ=255)                                                                                                                                                                                                                           cagcagGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGACTGCTTTTGCGCAGGCTCGGTTAGGATAAGAACAt                                                             >  2:75514/1‑300 (MQ=255)                                                                                                                                                                                                                           cagcagGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTAAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACAt                                                             >  1:70414/1‑300 (MQ=255)                                                                                                                                                                                                                                gggTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTtata                                                        >  1:688633/1‑300 (MQ=255)                                                                                                                                                                                                                                gggTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTtata                                                        >  2:757049/1‑300 (MQ=255)                                                                                                                                                                                                                                gggTTACGCCAACGCCAACGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTAGACAAGCGTGCGGAAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTtata                                                        >  2:157433/1‑300 (MQ=255)                                                                                                                                                                                                                                 ggTTACGCCAACGCCACCGAACTGGCGGATTNTCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGNTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTatat                                                       >  2:603269/1‑300 (MQ=255)                                                                                                                                                                                                                                 ggTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCTAAGCGCACCATATTGTTGTTGAAGCGGTGGTAGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCATGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGTTTAGGGTAAGAACATTTatat                                                       <  2:48470/300‑1 (MQ=255)                                                                                                                                                                                                                                 ggTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGTTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTatat                                                       >  1:757616/1‑300 (MQ=255)                                                                                                                                                                                                                                 ggTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTatat                                                       >  2:82036/1‑300 (MQ=255)                                                                                                                                                                                                                                 ggTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTatat                                                       >  1:1935/1‑300 (MQ=255)                                                                                                                                                                                                                                 ggTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTAAACGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTatat                                                       >  2:379100/1‑300 (MQ=255)                                                                                                                                                                                                                                      cgccaacgccaCCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTATATGtata                                                  >  1:369583/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTATATGTATAAATTTGAGCCTGGCTTATc                               <  1:783843/300‑1 (MQ=255)                                                                                                                                                                                                                                                          cGGATTATCTGGTGGCGAAAGTCGTACCGTTCCGCGACGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTATATGTATAAATTTGAGCCTGGCTTATCg                              <  1:765113/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       ggCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCGGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTATATGTATAAATTTGAGCCTGGCTTATCGCCGGGCTTTTTTa                 >  1:472059/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       ggCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTATATGTATAAATTTGAGCCTGGCTTATCGCCGGGCTTTTTTa                 >  1:255377/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       ggCGAAAGGCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGACGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTATATGTATAAATTTGAGCCTGGCTTATCGACGGGCTTTTTTa                 >  2:499629/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               gCGTACCGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACACTTATATGTATAAATTTGCGCCTGGCTTATCGCCGGGCTTTTTTATGGCaaaa         >  2:415815/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    ccGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTNTATGTATAAATTTGAGCCTGGCTTATCGCCGGGCTTTTTTATGGCAANAAAAAg    >  2:688068/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    ccGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTATATGTATAAATTTGAGCCTGGCTTATCGCCGGGCTTTTTTATGGCAAAAAAAAg    >  2:633064/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    ccGTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTATATGTATAAATTTGAGCCTGGCTTATCGCCGGGCTTTTTTATGGCAAAAAAAAg    >  1:140961/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gTTCCGCGAAGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCCGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTATATGTATAAATCTGAGCCTGGCTTATCGCCGGGCTTTTTTATGGCAAAAAAAAGCg  >  2:328975/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                   CGCTGGAGCTGATTCGCGGTAAATGCGGCCGGGTGCAGGGGGCGTTAACCGGCATGATGATGACGCTGAAAGGTTTGCCGCTGGCTTACAACAAAGATATGCAGGAAGACAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGATTGCTTTTGCGCAGGCTCGGTTAGGGTAAGAACATTTATATGTATAAATTTGAGCCTGGCTTATCGCCGGGCTTTTTTATGGCAAAAAAAAGCG  >  NC_000913/4157712‑4158288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑