Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,076,997	C→A	R629L (CGC→CTC)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,076,997	0	C	A	100.0%	202.5 / NA	55	R629L (CGC→CTC)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/28);  total (27/28)

ATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAAATACGACACGGACGGTTAAGTTT  >  NC_000913/1076703‑1077289                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     aTACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTAACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGgtgt                                                                                                                                                                                                                                                                                                 >  1:408371/1‑300 (MQ=255)    ccAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCg                                                                                                                                                                                                                                                                                              <  1:792986/300‑1 (MQ=255)                tgcATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATcc                                                                                                                                                                                                                                                                                  <  1:753602/300‑1 (MQ=255)                     cTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGccc                                                                                                                                                                                                                                                                             <  1:125824/300‑1 (MQ=255)                              cATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGtt                                                                                                                                                                                                                                                                    >  1:94573/1‑300 (MQ=255)                                   gCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTcgc                                                                                                                                                                                                                                                               >  1:743049/1‑300 (MQ=255)                                    cACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTcgcg                                                                                                                                                                                                                                                              <  1:743268/300‑1 (MQ=255)                                                   cAAAACCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAg                                                                                                                                                                                                                                               <  2:304148/300‑1 (MQ=255)                                                    aaaaTCGCTGCGCGTTCAGCCGTAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCTTGACCGTAAAGa                                                                                                                                                                                                                                              <  2:317444/300‑1 (MQ=255)                                                                      gCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGACCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAAtt                                                                                                                                                                                                                            >  1:382495/1‑300 (MQ=255)                                                                                                    ttattaACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGNCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTcc                                                                                                                                                                                              >  1:611442/1‑300 (MQ=255)                                                                                                                cTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCACGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGt                                                                                                                                                                                  >  2:68639/1‑300 (MQ=255)                                                                                                                           cTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTAcagc                                                                                                                                                                       <  1:373349/300‑1 (MQ=255)                                                                                                                             gttttACTTCACGCTGCGGTGCTTCACGCACATAGCCCACAATGTCTTTCGGTTCCTCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTAcagcag                                                                                                                                                                     <  2:44817/296‑1 (MQ=255)                                                                                                                             gTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTAccgcag                                                                                                                                                                     >  1:240986/1‑300 (MQ=255)                                                                                                                             gTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTAcagcag                                                                                                                                                                     >  1:379097/1‑300 (MQ=255)                                                                                                                                  aCTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGAcc                                                                                                                                                                >  1:648277/1‑300 (MQ=255)                                                                                                                                            gcgTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGa                                                                                                                                                      >  1:170781/1‑300 (MQ=255)                                                                                                                                                 gCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGt                                                                                                                                                 >  1:829593/1‑300 (MQ=255)                                                                                                                                                    tCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCNGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCa                                                                                                                                              >  1:573280/1‑300 (MQ=255)                                                                                                                                                     cACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCAt                                                                                                                                             >  1:74510/1‑300 (MQ=255)                                                                                                                                                       cacacaTAGCCCACAATATCTTTCGGTTCCGCATGGTTAATAACGTGCGACATCGCACCTGCCACTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATcc                                                                                                                                           <  2:175380/298‑1 (MQ=255)                                                                                                                                                       cGCACANAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCNCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTCTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATcc                                                                                                                                           <  2:716652/300‑1 (MQ=255)                                                                                                                                                                 ccACAATAGCTTTCGGTTCCGCATGGTTAATAACGGGCTACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTTGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGTTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAag                                                                                                                                 <  2:288708/300‑1 (MQ=255)                                                                                                                                                                  cacaATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAaga                                                                                                                                >  1:220509/1‑300 (MQ=255)                                                                                                                                                                    caATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAgg                                                                                                                              >  1:809257/1‑300 (MQ=255)                                                                                                                                                                      atatCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGgtg                                                                                                                            <  1:320195/300‑1 (MQ=255)                                                                                                                                                                                  tCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACgg                                                                                                                <  1:288488/300‑1 (MQ=255)                                                                                                                                                                                        gggTTAATGACGGGCGACATCTCACCTGCCGCTAACGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGNTCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCCGTTGCGCCAGTTTTTCTACAGCAGTGCCCGGATCGGCGACCAGTTTATCCAGTGGCAACAAGGTGTCGACAATACGGTTAACa                                                                                                          >  2:192668/1‑300 (MQ=255)                                                                                                                                                                                        gggTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACa                                                                                                          >  1:816046/1‑300 (MQ=255)                                                                                                                                                                                         ggTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCNCGCCCGTGACCGTAAAGATCGCGCGGNAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACaa                                                                                                         <  2:578981/300‑1 (MQ=255)                                                                                                                                                                                                      cGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGc                                                                                            <  1:504583/300‑1 (MQ=255)                                                                                                                                                                                                        aCATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCGTTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGANCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTACAGATCGCGCGGCAGGGGAATTTTCGGNTGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCAc                                                                                          <  2:691057/300‑1 (MQ=255)                                                                                                                                                                                                        aCATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGTAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCAc                                                                                          <  2:838482/300‑1 (MQ=255)                                                                                                                                                                                                        aCATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCAc                                                                                          <  1:453311/300‑1 (MQ=255)                                                                                                                                                                                                              cACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGtttt                                                                                    >  1:757499/1‑300 (MQ=255)                                                                                                                                                                                                                  tGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCNCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCcag                                                                                <  1:624715/300‑1 (MQ=255)                                                                                                                                                                                                                                gttCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCa                                                                  >  1:30410/1‑300 (MQ=255)                                                                                                                                                                                                                                        aTTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGcc                                                          <  1:237295/300‑1 (MQ=255)                                                                                                                                                                                                                                        aTTGGCAAGGCCTGCCATTTTTGCAGGGCACTATTGAGCAGGGCAGATGAGAGCGAGGCCAGTAGTTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTTTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAGTACGGTTAACAAACGAGGTGTTAGCATCGTTTTCCAGCAGGCGACGCACCAGATACGcc                                                          <  1:413695/300‑1 (MQ=255)                                                                                                                                                                                                                                           ggCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAAc                                                       <  1:303205/300‑1 (MQ=255)                                                                                                                                                                                                                                                                aGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGTTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCATGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAAcc                                  <  2:390411/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 gTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTAATGTGTGCCAACCg                                 >  1:316742/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 gTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGAAGCACCAGATACGCAAACAGCGTTTCATGTGTGCCGACCg                                 >  2:424151/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   gCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGa                               >  2:830543/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      cTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCa                            >  2:478102/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       tATTGAGCAGGGCAGAGGAGAGCGAGGCCAGTAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGGGACCGTAAAGATCGCGCGGCAGGGGAGTTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAATAGTTGTCGGCAATACGGTTAACAAACTAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCAt                           <  2:376781/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tGAGCAGGGCAGAGGAGAGCAAGGCCAGGAGGTGGTCGTTAGCGAGAGCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTNCTGTTGCGCCAGTTTTTCTACAGCATTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATCCGGTTAACAAACGAGGTGTTAGCATCGTTTGCCAGCAGGCGACGCACTAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATaaa                        <  2:192352/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tGAGCAGGGCAGAGGAAAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACTAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATaaa                        <  2:115145/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gggCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAAATACGac                  >  1:190279/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                gggCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAATTTGCCCTTCCTGTTGCGCCAGTTTTTATACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCCAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCAAACAGCGTTTCATGTGTGCCAACCGGAGCATAAATACGaa                  >  1:363032/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                gggCAGAGGAGAGCGAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGGTGTCGCGCCCGTGACCGTAAAGATCGAGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAAATACGac                  >  2:771754/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              gAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAAATACGACACGGACGGTTAAGt    <  2:318618/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              gAGGCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAAGTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAAATACGACACGGACGGTTAAGt    <  2:82324/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                ggCCAGGAGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAAATACGACACGGACGGTTAAGttt  >  1:832924/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     ATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAAATACGACACGGACGGTTAAGTTT  >  NC_000913/1076703‑1077289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑