| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,054,886 | 0 | G | T | 36.3% | 124.0 / 33.5 | 91 | Q187K (CAA→AAA) | ygfB | PF03695 family protein YgfB |
| Reads supporting (aligned to +/- strand): ref base G (15/43); new base T (33/0); total (48/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.07e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTG‑ATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAG > NC_000913/3054590‑3055183 | aGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACNGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAc < 1:569097/300‑1 (MQ=255) aacgcagttaaaaagaatacggtagttgtGAGTGTCAGCGCTGGGAATCAGAACCAGAACCGCTTCCGGGTCGGGAAAGCCGGTGAAGTACCAGAAGTCACTGTGCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGGGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCCTACGGTTTTTGTGTTTAGTGGAGAGTCGGGTTGTGTACTTCTGgcg < 2:330711/272‑1 (MQ=255) cgGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGGCGGTTTTTTTCCTTCTTGCGcggt > 1:648790/1‑300 (MQ=255) cgGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCAATCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCCTCTTGCGcggt > 1:175050/1‑300 (MQ=255) gTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCAATCATAACACTCTCATTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCCTCTGGCGcggtct > 2:349776/1‑299 (MQ=255) aaaCAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGGCGGTTTTTGTACTTCTGGCGCGGTCAGtt > 2:320298/1‑300 (MQ=255) agcGCTGTGGTTATGAGTGTCATCGCTTTGAATCAGCACCAGTACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGGCGGCGCTACGTGTGACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt < 2:111493/298‑1 (MQ=255) aaCGCTNTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGNTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt < 2:640283/300‑1 (MQ=255) cTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTTACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCCTGTGCGGTCTGTTTCAGATGAG‑t > 1:649434/1‑300 (MQ=255) gtgGTTNTGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTNAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑Taa < 2:637876/300‑1 (MQ=255) gtgGTTATGAGTGTCATCGCTTTTACTCAGCACCAGCAACGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTATTCTGACGATAGGGGTATTTGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCAGTGCCGGGTTGCATTTGCTCCACCAGGGACTGACGGCGCCGCTGAAACTCTTGCCGGGATATCTCACTCCTAACATTCTCATTACGTTTTTTGTTTTTAGTGTAGAGGGGGTTTTTGTACttattgtgtggtcgagtgtgggctaggca > 2:303003/1‑273 (MQ=255) gtgGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCAATCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCTTCTTGCGCGGTCTGTTGCTGATGAG‑Taa > 1:617181/1‑300 (MQ=255) ggTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCAGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCTTGTTCGGTCTGTTTCGGATGAG‑tcaaa > 1:734528/1‑296 (MQ=255) gtgaTGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAgg < 2:227621/297‑1 (MQ=255) gTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTGGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAgg < 1:667168/300‑1 (MQ=255) gTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAgg < 2:328677/300‑1 (MQ=255) gtgtCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCTGGCGCGGGCGGTTGCGGATTAG‑TAAAAGTGTCGtt > 2:432572/1‑299 (MQ=255) cGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAAc < 1:493827/300‑1 (MQ=255) cGCTTTNAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCNGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAAc < 2:638784/300‑1 (MQ=255) cTTTTAATCCGCACCAGCATCGTTTCCGGTTCTGTAAAGCCGGTGAAGTACCAGGAGTCACTGTTCTGACGATATGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCGAAGATCAGCGCGGCGCTGCCTGGTTGCATGTGCTCCACCAGGGCCTGACGGCGACGCTGAAGCTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACTTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAg < 2:18936/300‑1 (MQ=255) cTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCTTCTTGCGCGGTCGGTTTCGGATGAG‑GAAAGGTGTCGTTGCATAACAg > 1:687773/1‑300 (MQ=255) cTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAg < 1:752495/300‑1 (MQ=255) caccagcaccGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTTGGTTTCGGATGAG‑TAAAAGTGTCGTGGCATCACAACGCGGCAacac > 1:499044/1‑300 (MQ=255) gcaccGCTTCCGGTTCGTTAAAGCCGTTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACg < 2:413716/300‑1 (MQ=255) aaGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAg < 1:724941/300‑1 (MQ=255) aGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTTGTTTTTGTCCTTCTTGTGCGGTCGGTTTCTGGTGAG‑TAAAAGTGTCGTGGCATCACAACACGGCAACAC‑GAAAGTATTCGATGACCTCTTCAAGc > 2:432517/1‑300 (MQ=255) gCCGTTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCg < 1:73263/300‑1 (MQ=255) gCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCg < 1:69128/300‑1 (MQ=255) aaGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCNTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCAGTCGGTTGCGGGTGGG‑TAAAAGGGTCGTGGCCTAACAGCCCGG‑ACCAAAGAACGTATTCTATGATCTCTTCAAGCAACA‑CTTc > 2:179160/1‑300 (MQ=255) aGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGNCGCTGAAACTCTTGCCGGGATATCTCNCTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTTGTTTTTTTCCTTCTTTTTCGGGCGGTTTCGGATGAGGCAAAGG‑GTCGTGGCACCAAAACGCGGCAACAC‑AAACGTATTTGACGATCTCTTccaaagaaccttcc > 2:510208/1‑287 (MQ=255) gTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCTTGCGCGGTCGGTTGCGGATGAG‑TCAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑AAACGTACTCGATGACCTCCTCAAGCGAAA‑TTTCaa > 1:328517/1‑300 (MQ=255) aGTCACTGTTCTGACGATAGGGGTATTCGCTTTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTcc < 2:389479/300‑1 (MQ=255) tCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTGCTTCTTGTGCGGTCGGTTTCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGAAACAC‑AAACGTATTCGATGCTCTCTTCCAAAGACA‑TTTCAAGGTTTTCCTg > 2:156563/1‑300 (MQ=255) tCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCATTACGTTTTTTGTTTTTAGTGTAGAGTGGGTTTTTTTCCTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAAGTGTCTTGGCACCACCGCTCGGCACCCC‑AAACGTATTCGATGATCTCTTCAAGCAACA‑TTTCACGCTCTTCCTg > 1:24906/1‑300 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGNTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCNGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTGGGGTGGTCGGGTTTGGAGGAG‑TAAAGGTGTTGTGGCACAACAGCGCGGAAACAC‑GAAAGTATTTGATGATCTCTTCCAGCGACC‑CTTCCAGCTCTTCCTGAtctt > 2:543541/1‑300 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTTCTTCTTTTTCGGTTGGTTTCGTATGAG‑TAAAAGTGTCGTTGCCTCACAACACAGCAACCA‑AAACGTATTTGATTGTCTCTTCAAGCTACA‑TTTCCAGCTCTTCCttctttt > 1:61193/1‑294 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCTGTCGGTTTCTGATGAG‑TAGAGGTGTCTTTGCCCAACCGCGCGGCAACCC‑GAACGTATTCTATGATCTCTTCAAGCTACC‑CTTCCAACTCTTCCTTAtctt > 1:768085/1‑300 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCTGTTTTGGATTAG‑TAAAGGTGTCGTGGCACAACAGCGCGGCAACCC‑AAACGTATTCTATGATCTCTTCCAACAACC‑CTTCCAGCTCTTCCTGAtctt > 1:732240/1‑300 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGATCGGTTGGTTTCGGATGAG‑TCAAAGTGTCTTTGCCCCACCACGCGGCCACCC‑AAACTTCTTCTATTATCTCTTCCAACGACA‑TTTCCAGCTCTTCCTTAtctt > 2:590064/1‑300 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTTGCGCGGTCGGTTGCGGATGAG‑TAAAAGTGTCGTTGCATAACAACGCGGCAACAC‑GAACGTATTCTATGATCTCTTCCAGCGACA‑TTTCCCACTCTTCCTGGtctt > 1:331638/1‑300 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCAGTTTTTTTTCTTCTTGCGCAGTCGGTTGTGGATGAG‑TCAAAGTGTCGTTGCATCCCAACGCTGCAACAC‑AAACGTATTCAACGACCTCTTCCAGCGACA‑TTTCAAACTCTTCCTGaccct > 2:696861/1‑296 (MQ=255) ggTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGTGCTGTCGGTTTCGGATGAG‑TAAAGGTGTCGTGGCACCACAACACTGAAACAC‑AAACATATTCTATGATCTCTTCCAACGACACTTTCAA‑CTCTTCCTGATcttcttcttcgttgtca > 1:99716/1‑295 (MQ=255) tATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGTTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑cc < 2:413472/300‑1 (MQ=255) agtCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGGGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATCTCTCACTCATAACACTCTCCTTACGTTTTTTGTTNTTAGTGTAGAGTCGGTTTTTGTACTTNTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑ccc < 2:510607/298‑1 (MQ=255) aTTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTNGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTGGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑ccc < 1:516811/300‑1 (MQ=255) gcTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGt < 2:315139/300‑1 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGc < 1:104650/300‑1 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCTTCTTGCGCGGTCGGTTGCGGAGGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑AAACGTATTCGATGATCTCCTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGTGCAATGTTACGc > 1:619081/1‑300 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGc < 1:103957/300‑1 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCGTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGc < 1:719526/300‑1 (MQ=255) gtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAg < 1:261607/300‑1 (MQ=255) ttCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCTTGCGCGGTCGGTTGTGGATGAG‑TAAAGGTGTCGTGGCCTAACAGCGCTGCAACAC‑AAAAGTATTCGATGATCTCTTCCCACGACA‑TTTCCCGCTCTTCCTGATCTTCGTTTTCGTCGTAA‑AACAGCTGCGCAACGTTACGCAAAtcgt > 1:308166/1‑300 (MQ=255) gcagcaAAAACCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGTATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATGCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGc < 2:204462/300‑1 (MQ=255) gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTGGCGCTGTTAGTTGCTGATGAG‑TAAAGGTGTTGTGGCATAACAGCGCGGCAACCC‑AAACATATTCTATGATCTCTTCCCGCGACC‑TTTCCAACTCTTCCTGATCTTCGTCTTCGTCGTCA‑CCCCGTTgttgaacgttcctcagattgtcgaggggttc > 1:105847/1‑270 (MQ=255) aaaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCAcc < 1:740672/300‑1 (MQ=255) aaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGNTTTTTTCCTTCTTGCGCGGTTGGTTGTGGATGAG‑TCAAGGTGTCGTGGCATAACAACGCGGNAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCCAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTCCGCAGACCGTCGACAGCTTCAACgg > 2:610992/1‑300 (MQ=255) tCAGCGCGGCGCGGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGTTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGcctgtctcttatacacatctcgcag < 1:214045/300‑25 (MQ=255) gggTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCCTGCGCGGTCGGTTGCGGATGAG‑TAAAAGTGTCGTGGCACCACAACGCGGCAACAC‑AAACGTATTCTATGATCTCTTCCAACAACA‑CTTCCAACCCTTCCTTATCTTCGTCTTCTTCGTAA‑ACCAATTGTTAAATGTTAAACAGACCGTCGATAGCTTCCCCAGTTTCTCCGGTCACtttct > 1:499200/1‑298 (MQ=255) gggTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCTTGCGCGGTCGGTTGCGGATGGG‑TCAAGGTGTCTTGGCATCACAACGCGGCACCAC‑GAACGTATTTGATGATCTCTTCCAACAACA‑TTTCCAACTCTTCCTGACCTTCGTCTTCGTCGTAA‑ACCAGTTGCGCAACGTTCCACAAATCGTCAACAGCTTCACCAGTTTCGCCAGTCAccctat > 1:259310/1‑295 (MQ=255) gggTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCAGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTTTGGATGAG‑TCAAAGTGTCGTTGCACAACAACGCGGCAACAA‑GAACATCCTCGATGATCCCTTCCAACGACA‑CTTCAAACCCTTCCTGATCTTCGTCTTCGTCGTAAACCCA‑TTGTGAAAAGTTAAGAAGGTCAGCGATAGCTTCAACGGTTTCGACGGTCAATTTAt > 1:231952/1‑300 (MQ=255) ggTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACCTCTTGCACGGTCGGTTTCGGATGAG‑TAAAAGTGTCGTGGAATAACAACGCGGCAACAC‑GAACATATTCGATGATCTCTTCAAGCGAAA‑CTTCCAGCTCTTCCTGATCTTCTTCTTTGTCGTGA‑CCCAGTTTCGCAACGTTCCGCAAATCTTCGATAGCTTCACCAGTTTTGCCgggccccttctc > 1:217122/1‑290 (MQ=255) gTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAANGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGANAGCTTCACCGGTTTCGCCGGTCACTTTATcc < 2:550587/300‑1 (MQ=255) gTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATcc < 1:484534/300‑1 (MQ=255) gCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCTGTTTTTGTCCTTCTTGCACAGTCGGTTTCGGACGAG‑TAAAGGTGTCGTGGCATAACAACGCGGCAACAC‑AAACGTATTCGATGATCTCTTCAAAAAACA‑TTTCCAACCCTTCCCTATCCTCGTCTTCGTCGTAA‑CCCAGTTTCGCCACGTTACACCGAACGTCGACAGTTTCCACGGTTTCGCCGGTCCCTTTCTcccaa > 1:287278/1‑297 (MQ=255) gCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGt < 1:677845/300‑1 (MQ=255) accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCTTCTTGTGCGGTCGGTTGTGGATGAN‑TAAAGGTGTCGTGGCACAACAACGCGNAAACAA‑GAACGTATTTGATGGTCTCTTCAAACGACA‑TTTCAAACTCTTCCTGATCTTCGTCTTCTTCGTAA‑ACCAGTTGTGCCATGTTCCGCAAATCGTCGATAGCTTCACCAGTTTTGCCGGTTACTTTATCCCGCCTCGGTTgtgt > 2:520541/1‑297 (MQ=255) accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTTCTGATGAG‑TCAAAGTGTCGTGGCCCCACAACGCAGCAACCC‑AAACATCTTCGATGGTCTCTTCAAACGACA‑CTTCCAACTCTTCCTGATCTTCGTCCTCGTCGTAA‑ACCAATTGTGCAATGTTCCACAGATCGTCGACAGCTTCCCCAGTTTCGCCAGTCACTTTCTCCAACTTCAGTTgtgt > 1:734421/1‑297 (MQ=255) accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCCTCTTGAGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCACAACAACACGGAAACAC‑GAACATATTCAATGATCTCTTCCAACGACA‑CTTCCAGCTCTTCCTTATTCTCGTCTTCGTCGTAA‑CCCCATTGCGCAATGTTCCGCAAATCGTCGATAGCTTCAACAGTTTCTCCAGTCACTTTCTCCCGCTTCGGTTTCGt > 1:681387/1‑300 (MQ=255) accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACCTCTTGCGCGGTCGGTTGCGGATGGG‑GAAAGGTGTCGTGGCATACCAACGCGGCAACAC‑GAACGTATTTTATGATCTCTTCCAGCGACA‑TTTCCAGCTCTTCCTGAGTTTCGTCTTCGTTGTCA‑CCCAGTTGTGCAATGTTCCGCAGATCGTCGATAGCTTCCCAAATTTCGCCGGTTCCCTTCTCCCGCTTCGGTTGCGt > 2:35854/1‑300 (MQ=255) gggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTCCTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAAGTGTCGTGGCACCACAACACAGCAACAC‑CAAAGTCTTCGATGATCTCTTCAAGCAACA‑TTTCCAGCTCTTCCTGATCTTCGTTTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAAATCGTCGATAGCTTCCACAGTTTCGCCGGTCACTTTCTCCCGCCTCTGTTGCGTaaaa > 1:6352/1‑298 (MQ=255) ggCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGc < 2:452594/300‑1 (MQ=255) aCGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGAc < 1:688148/300‑1 (MQ=255) aCGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGNTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGANAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGAc < 2:726207/300‑1 (MQ=255) ctTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTG‑ATTg < 1:266876/300‑1 (MQ=255) tgcctgtatatCTCACTCATAACACTCTCCTTACGTTTTTGGTTTGTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCTTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAATCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTG‑ATTGAc < 2:47679/293‑1 (MQ=255) gggATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTTGCGCGGTCGGTTTCGGATGAG‑TAAAAGTGTCGTGGCACAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCCAACGAAA‑TTTCCAGCTCTTCCTGGTCTTCGTCTTCGTCGTAA‑CCCAATTGCGAAATGTTCCGCAAATCGTCGATAGCTTCAACAGTTTCGACGGTCAATTTCTCCCGATTCGGTTTTGTAACGCCAAAAACAAACAAGAAGTGGTTTGACCCa > 1:643058/1‑300 (MQ=255) gATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAAAAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGTAAGTG‑ATTGACCCAAcc < 1:397518/300‑1 (MQ=255) atatCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTTGCACGGTCGGTTTCTGACGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCCTCCAACGACA‑CTTCCAACTCTTCCTGATCTTCGTCTTCGTCGTAA‑ACCAGTTGCGCAATGTTCCGCAGATCGTCGATAGCTTCACCAGTTTCGCCGGTCACTTTCTCCAGCTTCGGTTGCGTAACACCAAGACCAAACAGGAAGTG‑ATTGAccccaacc > 1:129949/1‑295 (MQ=255) atCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTACCTCTTGCGCGGTCGGTTGCGGATGAG‑TAAAAGTGTCGTGGCACCACAACGCGGAAACAC‑AAACGTATTTTATGATCTCTTCCAACAACA‑CTTCCAACTCTTCCTGATCTTCGTCTTCGTCGTAA‑ACCAATNGCGCAACGTTCCACAAATCGTCGATAGCTTCCCCAGTTTCGCCAGTCACTTTCTCCAGCTTCGGTTGCGTAACAACAAAAACAAACAGGGAGTG‑ATTGAACCaaacttc > 1:568895/1‑295 (MQ=255) atCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTTTCGCGGTCGGTTTCAAACAAA‑TAAAAGTGTCGTGGCACCACAACGCGGCAACAC‑AAACATCTTCGATTATCTCTTCCAACGAAA‑CTTCCAACTCTTCCTGATCTTCTTCTTCGTCGTAA‑CCCAGTTGCGACATGTTCCGCAAATCGTCGATAGCTTCCCCCGTTTCGACGGTCACTTTATCCCAATTCTGTTTCGTAACACCAAGACCAAGCAAGAAATG‑Atttaacccacctac > 1:264448/1‑288 (MQ=255) ctcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCTTCTTGCTCGGTCGGTTGTTGATGAG‑TCAAGGTGTCGTGGCATCACAGCAAGGCAACAA‑AAACATATTCTATGACCTCTTCCCACAACA‑TTTCAAACCCCTCCTGATTTTCGTCTTCGTCGTAA‑ACCAATTGCGAAACGTTACGCCGACCGTCGATAGCTTCACCAGTTTCGCCGGTCAATTTCTTCCGCTNCGGTTGTGTAAAAACAAAAACAAACAGGAAGTG‑ATTGACCCaaacttnccatgc > 2:631894/1‑289 (MQ=255) ctcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCTTCTTGCGTGGTCGGTTTCGGATGAG‑TAAAGGTGTCGTTGCATCACAGCGCAGCAACAC‑AAACATCTTCTATGGTCTCTTCCAGCGACA‑TTTCCAACTCTTTCTGAGCTTCGTTTTCGTCGTAA‑CCCCGTTGCGCCATGTTCCGCCAAGCGTTGATAGCTTCCCCTGTTTTGCCGGTCCCCTTCTCCAGCTTCGGTTGTGTAAAGACAAAAACAAACAGGAAGTG‑ATTGAACCaaactatcaacgc > 1:254974/1‑289 (MQ=255) ctcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTGCGGATGAG‑TAAAAGTGTCGTGGCATAACAACGCGGCAACAC‑AAACATATTCTATGATCTCTTCCAACGACA‑CTTCCAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑ACCAAGTGTGCAATGTTACGCAGAGCGTCGATAGCTTCACCGGTTTCGCCGGTCACCTTCTCCAACCTCGGTTTCGTAACAACAAAAACAAACAGGAAGTG‑GTTGGCCCAACCCGCCaacgc > 1:671195/1‑297 (MQ=255) ctcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCANGCTCTTCCTGATCTTCGTCTTCGTCGNAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTG‑ATTGACCCAACCTGCCAATGc < 2:523132/300‑1 (MQ=255) caTAACACTCTCCTTACGCTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTG‑ATTGACCCAACCTGCCAATGCAt < 1:48784/300‑1 (MQ=255) cacTCTCCTTACGTTTTTTGTTTTTAGTTTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTG‑ATTGACCCAACCTGCCAATGCATCAGcc < 2:699140/300‑1 (MQ=255) cGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTTCGGATGAG‑TAAAAGTGTCGTGGCATCACAACGCGGCAACAC‑GAACATATTCTATGATCTCTTCCAACGACA‑CTTCCAACTCTTCCCTATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTCCACAGATCGTCGATAGCTTCCCCAGTTTCTCCGGTCACTTTCTCCCGCTTCTGTTGCGTCACACCAAGACCAAGCAGGAAGTG‑ATTTACCCAAACTGCCAACGCCTCAGCCCAACCGaaaaa > 1:288712/1‑299 (MQ=255) gTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCTGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTG‑ATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGAcat < 2:415958/300‑1 (MQ=255) gTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTG‑ATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGc < 2:85607/300‑1 (MQ=255) gTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGNCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTG‑ATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGc < 2:174355/300‑1 (MQ=255) ttGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGNCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACNTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTG‑ATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAg > 2:609240/1‑300 (MQ=255) | AGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACAC‑GAACGTATTCGATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAA‑CCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTG‑ATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAG > NC_000913/3054590‑3055183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |