Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,092,996	(TGGC)4→3	coding (599‑602/1071 nt)	hisC →	histidinol‑phosphate aminotransferase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2092982	2092985	4	68 [0]	[0] 68	hisC	histidinol‑phosphate aminotransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2092986 =	0 (0.000)	68 (0.720)	55/570	0.4	100%	coding (589/1071 nt)	hisC	histidinol‑phosphate aminotransferase
	?	NC_000913	= 2092981	0 (0.000)					coding (584/1071 nt)	hisC	histidinol‑phosphate aminotransferase

CGCCATCCTCTACTGCCCGCCAACGTACGGCATGTACAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTT  >  NC_000913/2092700‑2093275                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                       cGCCATCCTCTACTGCCCGCCAACGTACGGCATGTACAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaa                                                                                                                                                                                                                                                                                      <  1:277551‑M2/300‑19 (MQ=255)     aTCCTCTACTGCCCGCCAACGTACGGCATGTNCAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCNGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatc                                                                                                                                                                                                                                                                                  <  2:555589‑M2/300‑23 (MQ=255)     aTCCTCTACTGCCCGCCAACGTACGGCATGTACAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTCCAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTCAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatc                                                                                                                                                                                                                                                                                  <  2:323416‑M2/300‑23 (MQ=255)       ccTCTACTGCCCGCCAACGTACGGCATGTACAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccg                                                                                                                                                                                                                                                                                <  1:190968‑M2/300‑25 (MQ=255)          ctACTGCCCGCCAACGTACGGCATGTACAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTACGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcac                                                                                                                                                                                                                                                                             <  1:164846‑M2/300‑28 (MQ=255)                    ccAACGTACGGCATGTACAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATNAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattt                                                                                                                                                                                                                                                                   <  2:190488‑M2/300‑38 (MQ=255)                        cGTACGGCATGTACAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTNGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacg                                                                                                                                                                                                                                                               <  1:516222‑M2/300‑42 (MQ=255)                             ggtatgtACAGCGTCGGCGCCGAAACGATTGGCGTCGGGTGCCGCACGGTGCCGACGCTGGGCAACTGGCAATTGGATTTACAGTGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacac                                                                                                                                                                                                                                                          >  2:26637‑M2/4‑254 (MQ=255)                             ggCATGTACAGCGTCAGCGCCGAAACGATTTGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATGGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCTCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacac                                                                                                                                                                                                                                                          >  2:355887‑M2/1‑254 (MQ=255)                             ggCATGTACAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacac                                                                                                                                                                                                                                                          <  1:315474‑M2/300‑47 (MQ=255)                               cATGTACAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCNGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactg                                                                                                                                                                                                                                                        <  2:573531‑M2/300‑49 (MQ=255)                                  gTACAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcg                                                                                                                                                                                                                                                     <  1:719974‑M2/300‑52 (MQ=255)                                     cAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaa                                                                                                                                                                                                                                                  >  2:313588‑M2/1‑246 (MQ=255)                                       gCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagc                                                                                                                                                                                                                                                >  1:777902‑M2/1‑244 (MQ=255)                                             gcgcCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgc                                                                                                                                                                                                                                          <  1:134601‑M2/300‑63 (MQ=255)                                               gcCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctc                                                                                                                                                                                                                                        >  1:195978‑M2/1‑236 (MQ=255)                                                         ttGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggct                                                                                                                                                                                                                              >  1:179963‑M2/1‑226 (MQ=255)                                                           ggCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGCCCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttc                                                                                                                                                                                                                            <  1:579687‑M2/300‑77 (MQ=255)                                                                   gTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcgga                                                                                                                                                                                                                    <  1:167140‑M2/300‑85 (MQ=255)                                                                          acaGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCTCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgc                                                                                                                                                                                                             >  2:203903‑M2/1‑209 (MQ=255)                                                                             gTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGANGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttangcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctgg                                                                                                                                                                                                          <  2:713838‑M2/300‑95 (MQ=255)                                                                                   aCGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTNAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacantgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacg                                                                                                                                                                                                    <  2:721173‑M2/300‑101 (MQ=255)                                                                                                        gACTTACAGGGCATTTCCGACAAGCTGGACGNCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGNTAAGGCGATTGTGGTTGCCGATGAAGGCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacatgctga                                                                                                                                                                               <  2:538194‑M2/300‑122 (MQ=255)                                                                                                           ttACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatga                                                                                                                                                                            <  1:445337‑M2/300‑125 (MQ=255)                                                                                                            tACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaa                                                                                                                                                                           >  1:53484‑M2/1‑175 (MQ=255)                                                                                                                  gCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgat                                                                                                                                                                     <  2:334656‑M2/300‑132 (MQ=255)                                                                                                                        ccGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgcccc                                                                                                                                                               >  2:445733‑M2/1‑163 (MQ=255)                                                                                                                             aaGCTGGACGGCGTAAAATTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAATGNGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCATGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacc                                                                                                                                                          >  1:516776‑M2/1‑158 (MQ=255)                                                                                                                             aaGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGNAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacc                                                                                                                                                          >  2:576968‑M2/1‑158 (MQ=255)                                                                                                                             aaGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacc                                                                                                                                                          >  1:72612‑M2/1‑158 (MQ=255)                                                                                                                              aGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctaccc                                                                                                                                                         >  1:701175‑M2/1‑157 (MQ=255)                                                                                                                                       gCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgac                                                                                                                                                <  1:86337‑M2/300‑153 (MQ=255)                                                                                                                                                     tATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgnacactttcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgcnccctacccgctctcgacgccggttgccgaca                                                                                                                                  >  2:554972‑M2/1‑134 (MQ=255)                                                                                                                                                     tATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCGGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgaca                                                                                                                                  >  2:422392‑M2/1‑134 (MQ=255)                                                                                                                                                           tGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGNGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcgg                                                                                                                            >  1:548780‑M2/1‑128 (MQ=255)                                                                                                                                                                  ccAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggc                                                                                                                     <  1:634052‑M2/300‑180 (MQ=255)                                                                                                                                                                   cAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcg                                                                                                                    <  1:603425‑M2/300‑181 (MQ=255)                                                                                                                                                                    aaTAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGTTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCNGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgt                                                                                                                   >  1:566922‑M2/1‑119 (MQ=255)                                                                                                                                                                        aCCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAANCCGCGGTAAGGCGATTGTGGTTGCCGAGGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctgtcggaatatccgcaccttgctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaag                                                                                                               >  2:574613‑M2/1‑115 (MQ=255)                                                                                                                                                                        aCCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaag                                                                                                               >  1:595158‑M2/1‑115 (MQ=255)                                                                                                                                                                        aCCCGACCGGGCAACTAATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaag                                                                                                               >  2:400431‑M2/1‑115 (MQ=255)                                                                                                                                                                           cGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagccc                                                                                                            >  1:641744‑M2/1‑112 (MQ=255)                                                                                                                                                                                tggcAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGGTAACCCGCGGTNAGTCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgcccnctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagg                                                                                                       >  2:728188‑M2/2‑107 (MQ=255)                                                                                                                                                                                gggCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagg                                                                                                       >  1:431464‑M2/1‑107 (MQ=255)                                                                                                                                                                                   cAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaa                                                                                                    <  1:755781‑M2/300‑197 (MQ=255)                                                                                                                                                                                     aCTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatc                                                                                                  >  1:770551‑M2/1‑102 (MQ=255)                                                                                                                                                                                                                 ctgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaa                                                                      <  1:145915‑M2/300‑227 (MQ=255)                                                                                                                                                                                                                 ctgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaa                                                                      <  2:350179‑M2/300‑227 (MQ=255)                                                                                                                                                                                                                   gctgGAGTTACCCCACGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgctgcggatttacgctggcaaacgaagaagtcatcaacttgttgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccaaagggaatcgtcgccatgcgagaaggggtagtgcaactt                                                                    <  2:389027‑M2/300‑229 (MQ=255)                                                                                                                                                                                                                      ggAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaatgggtagcgcaaattatc                                                                 <  2:387567‑M2/300‑232 (MQ=255)                                                                                                                                                                                                                       gAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattg                                                                <  2:52778‑M2/300‑233 (MQ=255)                                                                                                                                                                                                                        aGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgc                                                               >  1:727533‑M2/1‑67 (MQ=255)                                                                                                                                                                                                                                  cgGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgncccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaa                                                     <  1:553059‑M2/300‑244 (MQ=255)                                                                                                                                                                                                                                  cgGTAAGGCGATTGTGGTTGCCGATGAAGCCNATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggnggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccctgcgcgaacgggtagcgcaaattattgcagaacgcgaa                                                     <  2:562709‑M2/300‑244 (MQ=255)                                                                                                                                                                                                                                      aatgcgaTTTTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCACAGGCATCgctggctggctggcggaatatccgcacccggctattttacgcacactgtcgaaagcttttgctctggcggtgcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacc                                                 >  2:60877‑M2/4‑53 (MQ=255)                                                                                                                                                                                                                                           gATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacctgatt                                            <  1:507893‑M2/300‑253 (MQ=255)                                                                                                                                                                                                                                            aTTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacctgattg                                           <  2:319960‑M2/300‑254 (MQ=255)                                                                                                                                                                                                                                                        gATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatatctgattgcagcactgaaag                               <  2:270319‑M2/300‑266 (MQ=255)                                                                                                                                                                                                                                                         aTGAAGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgntggcaaacgaagaagtcatcaacctgntgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgacatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacctgattgccgcactgaaaga                              <  2:526022‑M2/300‑267 (MQ=255)                                                                                                                                                                                                                                                          tGAAGTCTATATCGATTTTTGCCCGCAGGCATCgcgggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgcccccgacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagtgaatcgtcgccatgcgcgaatgggtagcgcaaattattgcagaacgcgaatacctgattgccgcactgaaagag                             >  2:65501‑M2/1‑33 (MQ=255)                                                                                                                                                                                                                                                             aGCCTATATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacctgattgccgcactgaaagagatc                          <  1:732993‑M2/300‑271 (MQ=255)                                                                                                                                                                                                                                                              gCCTAGATCGAGTTTTGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggtggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacctgattgccgcactgaaagagatcc                         >  2:12293‑M2/1‑29 (MQ=255)                                                                                                                                                                                                                                                                          ttttGCCCGCATGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggctgggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacctgattgccgcactgaaagagatcccctgcgtagagc             >  1:479687‑M2/1‑17 (MQ=255)                                                                                                                                                                                                                                                                          ttttGCCCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacctgattgccgcactgaaagagatcccctgcgtagagc             >  1:146675‑M2/1‑17 (MQ=255)                                                                                                                                                                                                                                                                          ttctgccCGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcatactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacctgattgccgcactgaaagagatcccctgcgtagagc             >  2:4081‑M2/4‑17 (MQ=255)                                                                                                                                                                                                                                                                               cccGCAGGCATCgctggctggctggcggaatatccgcaccttgctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacctgattgccgcactgaaagagatcccctgcgtagagcaggtt        >  1:629536‑M2/1‑12 (MQ=255)                                                                                                                                                                                                                                                                               cccGCAGGCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttgctctggcggggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaagtgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacctgattgccgcactgaaagagatcccctgcgtagagcaggtt        >  1:628012‑M2/1‑12 (MQ=255)                                                                                                                                                                                                                                                                                     ggCATCgctggctggctggcggaatatccgcacctggctattttacgcacactgtcgaaagcttttactctggctgggcttcgttgcggatttacgctggcaaacgaagaagtcatcaacctgctgatgaaantgatcgccccctacccgctctcgacgccggttgccgacattgcggcccaggcgttaagcccacagggaatcgtcgccatgcgcgaacgggtagcgcaaattattgcagaacgcgaatacctgattgccgcactgaaagagatcccctgcgtagagcaggttttcgac  >  2:190776‑M2/1‑6 (MQ=255)                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                       CGCCATCCTCTACTGCCCGCCAACGTACGGCATGTACAGCGTCAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTT  >  NC_000913/2092700‑2093275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑