Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,907,439	IS1 (+) +8 bp	intergenic (‑4/+2)	cspC ← / ← yobF	transcription antiterminator and regulator of mRNA stability CspC/DUF2527 domain‑containing protein YobF

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1907439 =	3 (0.030)	100 (1.010)	70/598	0.2	97.0%	intergenic (‑4/+9)	cspC/yobF	transcription antiterminator and regulator of mRNA stability CspC/DUF2527 domain‑containing protein YobF
	?	NC_000913	1978503 =	NA (NA)					noncoding (768/768 nt)	IS1	repeat region
*	?	NC_000913	= 1907446	3 (0.030)	81 (0.820)	57/598	0.4	96.3%	intergenic (‑11/+2)	cspC/yobF	transcription antiterminator and regulator of mRNA stability CspC/DUF2527 domain‑containing protein YobF
	?	NC_000913	= 1979270	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

AATCGTGTTGAGGCCAACGCCCATAATGCGGGCTGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAA  >  NC_000913/1978976‑1979275                                                                                                                                                                                                                                                                                                       |      aaTCGTGTTGAGGCCAACGCCCATAACGCTTGCTGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAACGATTTTCTGTTGCGTACCGGGTTGAGAAGCGGTGTAAGGGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCAcccgcga  <  2:211094/300‑5 (MQ=2)                                                                                                                                                                                                                                                                                                       |      AATCGTGTTGAGGCCAACGCCCATAATGCGGGCTGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAA  >  NC_000913/1978976‑1979275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.