Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,150,948	IS1 (+) +9 bp	coding (30‑38/1947 nt)	yegI ←	putative serine/threonine‑protein kinase YegI

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	128 (1.300)	82/598	0.1	97.6%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	2150948 =	3 (0.030)					coding (38/1947 nt)	yegI	putative serine/threonine‑protein kinase YegI
*	?	NC_000913	= 1979270	NA (NA)	84 (0.850)	64/596	0.3	96.5%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 2150956	3 (0.030)					coding (30/1947 nt)	yegI	putative serine/threonine‑protein kinase YegI

AATCGTGTTGAGGCCAACGCCCATAATGCGGGCTGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAA  >  NC_000913/1978976‑1979275                                                                                                                                                                                                                                                                                                       |      aaTCGTGTTGAGGCCAACGCCCATAACGCTTGCTGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAACGATTTTCTGTTGCGTACCGGGTTGAGAAGCGGTGTAAGGGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCAcccgcga  <  2:211094/300‑5 (MQ=2)                                                                                                                                                                                                                                                                                                       |      AATCGTGTTGAGGCCAACGCCCATAATGCGGGCTGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAA  >  NC_000913/1978976‑1979275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.