Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,887	G→A	A293A (GCC→GCT)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,887	0	G	A	100.0%	173.0 / NA	51	A293A (GCC→GCT)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/18);  total (33/18)

CGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAA  >  NC_000913/1829604‑1830158                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                cGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCACGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCAAGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTg                                                                                                                                                                                                                                                                 >  2:265589/1‑300 (MQ=255)          tGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGc                                                                                                                                                                                                                                                        >  1:303631/1‑300 (MQ=255)               gACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTgc                                                                                                                                                                                                                                                   >  1:66134/1‑300 (MQ=255)                      gCGGCATCGGCGTCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAGAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCt                                                                                                                                                                                                                                            <  1:614646/300‑1 (MQ=255)                      gCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCt                                                                                                                                                                                                                                            <  1:198523/300‑1 (MQ=255)                      gCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACACTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCt                                                                                                                                                                                                                                            <  1:614614/300‑1 (MQ=255)                          catctgcatcATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGccc                                                                                                                                                                                                                                        <  2:321436/300‑1 (MQ=255)                                            gccATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCATTCTGCCACTTCCACCAg                                                                                                                                                                                                                      >  2:705740/1‑300 (MQ=255)                                             ccATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGc                                                                                                                                                                                                                     >  1:638907/1‑300 (MQ=255)                                                                                  cgcgATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTcac                                                                                                                                                                                <  2:669970/300‑1 (MQ=255)                                                                                    cgATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGCCTTTTACGGATGGCGCGCACGCGGTCGATGTCacac                                                                                                                                                                              >  1:359813/1‑300 (MQ=255)                                                                                    cgATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCacac                                                                                                                                                                              <  2:4995/300‑1 (MQ=255)                                                                                    cgATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCacac                                                                                                                                                                              >  1:320663/1‑300 (MQ=255)                                                                                    cgATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCAGGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGACATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCacac                                                                                                                                                                              <  2:250800/300‑1 (MQ=255)                                                                                         cgcCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAg                                                                                                                                                                         <  1:158185/300‑1 (MQ=255)                                                                                          gcCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGc                                                                                                                                                                        >  1:660868/1‑300 (MQ=255)                                                                                          gcCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCAAACTCAAGc                                                                                                                                                                        >  2:709479/1‑300 (MQ=255)                                                                                                           aGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGTCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCaaa                                                                                                                                                       <  2:575415/300‑1 (MQ=255)                                                                                                                ccGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACCGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGCTGGCGCGCACGCGGTCGCTGTCACATTCCCGCGTCGGCCCACCGTCAAAGATGt                                                                                                                                                  >  1:682219/1‑300 (MQ=255)                                                                                                                ccGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGCGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGATGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCCGCCGACTTTTACGGATGGCGCGCATGCGGTCGATGTCAAACTAAAGCGTCGGCCTAACGTCacaggtgt                                                                                                                                                  >  2:253622/1‑293 (MQ=255)                                                                                                                ccGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGt                                                                                                                                                  >  1:330967/1‑300 (MQ=255)                                                                                                                ccGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGt                                                                                                                                                  >  2:375255/1‑300 (MQ=255)                                                                                                                        ccATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAg                                                                                                                                          <  1:379308/300‑1 (MQ=255)                                                                                                                           tAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTNATAATTTTCATTAGCGACCAGGCAGGNTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATTTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTa                                                                                                                                       <  2:489314/300‑1 (MQ=255)                                                                                                                               gTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGt                                                                                                                                   >  2:339961/1‑300 (MQ=255)                                                                                                                                       gTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGCCTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGaaaa                                                                                                                           >  2:41605/1‑300 (MQ=255)                                                                                                                                       gTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGaaaa                                                                                                                           >  1:584418/1‑300 (MQ=255)                                                                                                                                       gTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCATTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTAGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGaaaa                                                                                                                           >  1:136830/1‑300 (MQ=255)                                                                                                                                                tctGCGCCCAGGCGCACCCGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGCACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGTGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCAGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCAACGTAAAAGATGTCGATATAGTTACGGTAGCGAAAACCtgcttta                                                                                                                  >  2:421607/1‑299 (MQ=255)                                                                                                                                                      cacaGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGc                                                                                                            <  1:122698/300‑1 (MQ=255)                                                                                                                                                        caGGCGCACCAGACGAACGCGATCCCTTGCGTGTCATTTCAGGGCATCCAGTTGTGCTGCGGTTAAAATCAAACGCTCGGTTGCCAGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATGAGCGACCATGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCTACTTTTACGGATGGCGCGCACGCGGTCGATGTCGCACTCAAGCGTCTGCCCACCGTCAAAGATGTCGATATAGTTACGTTAGCGAAAACCTTCTTTCTCCAGCAc                                                                                                          <  2:187689/300‑1 (MQ=255)                                                                                                                                                        caGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCATTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCAc                                                                                                          <  2:379055/300‑1 (MQ=255)                                                                                                                                                        caGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCAc                                                                                                          <  2:50964/300‑1 (MQ=255)                                                                                                                                                        caGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGGGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCAc                                                                                                          <  1:302235/300‑1 (MQ=255)                                                                                                                                                              cACCAGACGAACGCGATCCCCGGCGTGGCATTTAAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCAACACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGACCTGCGCAGGCTGCCCTTCTGCCCCTTCCACCAGCCGACTTTTACGGATGGCGGGCACGCGGTCGATGTCAAACTCAAGCGTCGGCCCACCGTCGAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACcgagcg                                                                                                    >  2:206615/1‑300 (MQ=255)                                                                                                                                                                                           aTTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGAc                                                                       >  1:65307/1‑300 (MQ=255)                                                                                                                                                                                                   gCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACg                                                               >  1:134120/1‑300 (MQ=255)                                                                                                                                                                                                    cATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGt                                                              >  2:659722/1‑300 (MQ=255)                                                                                                                                                                                                        cAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTg                                                          >  1:306143/1‑300 (MQ=255)                                                                                                                                                                                                          gTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTggg                                                        >  1:135351/1‑300 (MQ=255)                                                                                                                                                                                                                    ggTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGa                                              >  1:109855/1‑300 (MQ=255)                                                                                                                                                                                                                                     cGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGa                             <  1:702127/300‑1 (MQ=255)                                                                                                                                                                                                                                       gTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATc                           >  1:323916/1‑300 (MQ=255)                                                                                                                                                                                                                                       gTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCAAACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATc                           >  1:40084/1‑300 (MQ=255)                                                                                                                                                                                                                                             ggATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACNTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATNGGATGt                     >  2:608607/1‑300 (MQ=255)                                                                                                                                                                                                                                                   gTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCCAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTATTCCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGc               >  1:6819/1‑300 (MQ=255)                                                                                                                                                                                                                                                   gTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGc               >  1:9404/1‑300 (MQ=255)                                                                                                                                                                                                                                                        caccagcaccaCGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCAGCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTNCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTAGGCATCAg          >  2:640254/1‑300 (MQ=255)                                                                                                                                                                                                                                                        caccagcaccaCGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAg          >  1:274974/1‑300 (MQ=255)                                                                                                                                                                                                                                                        caccagcaccaCGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCAAACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAg          >  2:226742/1‑300 (MQ=255)                                                                                                                                                                                                                                                                ccacGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCaa  <  2:65937/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                CGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAA  >  NC_000913/1829604‑1830158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑