Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,512,767	C→T	A215T (GCG→ACG)	fecC ←	ferric citrate ABC transporter membrane subunit FecC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,512,767	0	C	T	100.0%	208.5 / NA	60	A215T (GCG→ACG)	fecC	ferric citrate ABC transporter membrane subunit FecC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/28);  total (32/28)

CCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCT  >  NC_000913/4512472‑4513059                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                     ccTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCTCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTcagc                                                                                                                                                                                                                                                                                                  <  2:363002/300‑1 (MQ=255)             tctcCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCTCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCACCCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGaaa                                                                                                                                                                                                                                                                                      >  2:732479/1‑298 (MQ=255)             tctcCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCTCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACa                                                                                                                                                                                                                                                                                      >  2:758380/1‑300 (MQ=255)                  ggggAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCTCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGAc                                                                                                                                                                                                                                                                                 <  2:375460/300‑1 (MQ=255)                   gggAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCTCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACt                                                                                                                                                                                                                                                                                >  1:509506/1‑300 (MQ=255)                       aGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCTCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACTTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAg                                                                                                                                                                                                                                                                            <  1:491627/300‑1 (MQ=255)                          ccAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCTCCAGCAGCANGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCNGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGa                                                                                                                                                                                                                                                                         <  2:785223/300‑1 (MQ=255)                                       gAGTACATCTGCCAGCAGCATCAGCGTGGCCTCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTAGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCaa                                                                                                                                                                                                                                                            >  2:498826/1‑300 (MQ=255)                                                     gcagcaTCAGCGTGGCCTCCAGCATCATGCTCACTGGCAGGACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCATAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACAt                                                                                                                                                                                                                                              <  2:715746/300‑1 (MQ=255)                                                      cagcaTCAGCGTGGCCTCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCATCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATc                                                                                                                                                                                                                                             >  1:496633/1‑300 (MQ=255)                                                              gCGTGGCCTCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCAACGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCCCTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGCCCACCACCGGCAAGAGCTGCCAGACATCCTGCAAGc                                                                                                                                                                                                                                     >  2:466271/1‑300 (MQ=255)                                                                   gcctccAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGGTGAACAAACGTAGCCTAGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCAGCAAGAGCTGCAAGACATCCTGCCAGCGGGCg                                                                                                                                                                                                                                >  2:350905/5‑300 (MQ=255)                                                                   gcctccAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCg                                                                                                                                                                                                                                <  2:422789/296‑1 (MQ=255)                                                                         agcagcATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGac                                                                                                                                                                                                                          <  1:703412/300‑1 (MQ=255)                                                                             gcaTGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACANATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTc                                                                                                                                                                                                                      <  2:199823/300‑1 (MQ=255)                                                                             gcaTGCTCACGGGCAGTACGTTTCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTTACGCACGCACCAACCAGAAGCAGCACTAACANATGGATGACCAAACTTAGCCTCTTCAGGTTCACTCCCAGCGTATGGTCGGTGCTTTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACGTGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTc                                                                                                                                                                                                                      <  2:201046/300‑1 (MQ=255)                                                                                  cTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGcat                                                                                                                                                                                                                 >  2:819890/1‑298 (MQ=255)                                                                                        ggCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCAACGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTgcaagc                                                                                                                                                                                                           >  2:448279/1‑300 (MQ=255)                                                                                              aCGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTaa                                                                                                                                                                                                     >  1:834625/1‑300 (MQ=255)                                                                                              aCGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCAAGCCAGTaa                                                                                                                                                                                                     >  1:834592/1‑300 (MQ=255)                                                                                              aCGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCATGCAAGCAAGTaa                                                                                                                                                                                                     >  1:422895/1‑300 (MQ=255)                                                                                                   gcgcTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTaaaaaat                                                                                                                                                                                                <  1:193449/300‑4 (MQ=255)                                                                                                   gcgcTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGAt                                                                                                                                                                                                <  1:448518/300‑1 (MQ=255)                                                                                                        gATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATCGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCTTCATCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGt                                                                                                                                                                                           >  1:773139/1‑300 (MQ=255)                                                                                                                 cTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCCACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCCGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGAt                                                                                                                                                                                  >  1:776020/1‑300 (MQ=255)                                                                                                                  tGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACNAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATc                                                                                                                                                                                 <  2:755219/300‑1 (MQ=255)                                                                                                                   gCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGCGGGCCACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCt                                                                                                                                                                                >  2:317253/1‑300 (MQ=255)                                                                                                                              cgcCAGATGTGGCACCAGCCGACCGATAAACGCCACCGGACCTGCTCCGCTGACGCACGCACCAACCAAAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCCGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGATGAGGCTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCAAGACATCCTGCAAGCGGGCGTGGGACACTCAGCCTGCCAGCAAGTAAAAGATACCGTAAGCATGATCTTCGGCaagcag                                                                                                                                                                     >  2:381452/1‑300 (MQ=255)                                                                                                                                   gATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCACCACGACAGGGACTGCAGTGACCACCAACGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAggg                                                                                                                                                                >  1:326196/1‑300 (MQ=255)                                                                                                                                    aTGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAATCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGt                                                                                                                                                               <  2:127340/300‑1 (MQ=255)                                                                                                                                    aTGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGt                                                                                                                                                               >  2:822676/1‑300 (MQ=255)                                                                                                                                     tgtgGCACCAGCAGACCGATAAACGCCACCAGACCTGCTACGCTGACGCACGCACCAACCAGAGGCAGCACTAACATATTGAGGACCAAACTTAGCCTCGTCAGGTTCACTCCCAGCGTGTGGTCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCAGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTg                                                                                                                                                              <  2:330293/300‑1 (MQ=255)                                                                                                                                           aCCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCgg                                                                                                                                                        <  1:471049/300‑1 (MQ=255)                                                                                                                                                   aCCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCATAAGCAGCACTAACATATTGATGACCAAACCTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTGCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACACCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGcc                                                                                                                                                <  1:353019/300‑1 (MQ=255)                                                                                                                                                   aCCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGcc                                                                                                                                                <  1:327716/300‑1 (MQ=255)                                                                                                                                                                       cTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTAAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAGAAGATGCCGTAAGCATGATCTTCGGCAAGCAGCAGGGTGATGCGGGTAAGGACCATACAAAAGGCCGACAgcg                                                                                                                            >  2:432599/1‑300 (MQ=255)                                                                                                                                                                                  acgcacgcACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCcgcg                                                                                                                 <  1:252600/300‑1 (MQ=255)                                                                                                                                                                                   cgcacgcaCCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGa                                                                                                                >  2:515814/1‑300 (MQ=255)                                                                                                                                                                                   cgcacgcaCCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCAACGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCACATACAAAAGGCCGACAGCGCGATACCcgcgc                                                                                                                >  2:761418/1‑299 (MQ=255)                                                                                                                                                                                                        gcaCTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCAt                                                                                           >  1:645026/1‑300 (MQ=255)                                                                                                                                                                                                             aaCATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCACCAGCAGGGTGATGCGGGTACGGCACATNCAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTa                                                                                      >  2:620587/1‑300 (MQ=255)                                                                                                                                                                                                               cATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATg                                                                                    >  1:716385/1‑300 (MQ=255)                                                                                                                                                                                                                  atTGCTGACCAAACGTAGCCTCATCGGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTGGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACTACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCTTAAGCATGATCTTTGGCCAGCAGCAGGGTGATGCGGTTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACg                                                                                 <  2:291026/300‑1 (MQ=255)                                                                                                                                                                                                                                          cAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTGGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCATTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTTGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGAc                                                         <  2:251912/300‑1 (MQ=255)                                                                                                                                                                                                                                                cACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACcagcag                                                   <  1:695429/300‑1 (MQ=255)                                                                                                                                                                                                                                                        gCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCa                                           >  1:636365/1‑300 (MQ=255)                                                                                                                                                                                                                                                            aTGGTCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAANACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTTGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTNTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCAcgcc                                       <  2:791094/300‑1 (MQ=255)                                                                                                                                                                                                                                                            aTGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCATTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCAcgcc                                       <  2:244492/300‑1 (MQ=255)                                                                                                                                                                                                                                                            aTGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCAcgcc                                       <  1:278025/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   gTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGNCAGCCAGTAAAAGATGCCGTAAGCATNATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCg                                >  2:578121/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          cGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAACAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCACGCATGCcg                         >  2:286246/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          cGCTGAGGTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGAGTAAGGCACATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGCATGACGAAATACGCCTACTACGGTCATGACCAGCAGCCAGCTCACACCGCCCACGCATGCcg                         >  2:538325/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                ggTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCATCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGatga                   >  1:778282/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 gTTGAGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTACTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGatgaa                  >  2:774161/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     aGCAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTTGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGAc              <  2:758019/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       cAGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACag            <  1:283332/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        aGGTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACaga           >  1:738521/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         ggTTCAGTTGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACagag          >  2:811893/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                ttGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCTTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGcc   <  2:488635/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 tGATTCGTCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAATGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCt  <  2:349630/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                     CCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCT  >  NC_000913/4512472‑4513059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑