Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,946	G→A	W386* (TGG→TGA)	yagH →	putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,946	0	G	A	98.3%	202.0 / NA	60	W386* (TGG→TGA)	yagH	putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (31/28);  minor base C (1/0);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGC  >  NC_000913/287667‑288241                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        cGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGAACCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTAGGGCTCTATGGCAACGAATCGCTCAATTNGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGc                                                                                                                                                                                                                                                                                     >  2:626022/1‑300 (MQ=255)      gCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGAACGTGGCGCGCCGCTGACAGCACTTCGCCTTACGGGCAGaaa                                                                                                                                                                                                                                                                                >  2:413265/1‑300 (MQ=255)                 cgcgACGGCTGGCCATACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGTGCAGAAACGCGGATGGAg                                                                                                                                                                                                                                                                     <  2:81449/300‑1 (MQ=255)                       ggCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCNGGAGCTGCAGACCCTGCGCATTCCGTNCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCg                                                                                                                                                                                                                                                               >  2:577462/1‑300 (MQ=255)                         cTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGcc                                                                                                                                                                                                                                                             <  1:262045/300‑1 (MQ=255)                                tACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCNGACCCTGCGCATTCCGTTCGACGACANCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCAc                                                                                                                                                                                                                                                      >  2:581736/1‑300 (MQ=255)                                   gTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACTGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTc                                                                                                                                                                                                                                                   <  2:59582/300‑1 (MQ=255)                                         ggcggcAAACACACGCAGCCTACCGTGAAATGCCCGCAAGTAGCCGAACAGCCTGCAGCCGTTCCGGGCAACTTGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGTGCTTCTTACGGCTCTATGGCAACTACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCATAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcagcag                                                                                                                                                                                                                                             <  2:390801/300‑1 (MQ=255)                                           cggcAAGCATGCGCAGCTGGCCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGACAACTGGCGGGACGATTTCGACTCCCTTTCGCTTGACCCTGAGCTGCAGACCCTGCGCCTTCATTTCGACGACACCTTCTGCTCGCTCACCGCGCGAAGGGNCTTCTTCCGGCGCTATGGCAACAAATCGCTCAATCCGACCTTCCCCCAATCGACCATGAAGCGCCGCTGCCGACACCTCGAATTCCGAACAGAAACGCGGACAGAGTCCCCGCCGATGCACTTCcagtagcg                                                                                                                                                                                                                                           >  2:205608/1‑295 (MQ=255)                                                        cAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAANTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCNGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAcc                                                                                                                                                                                                                              >  2:785923/1‑300 (MQ=255)                                                          gCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTg                                                                                                                                                                                                                            <  1:18845/300‑1 (MQ=255)                                                                  ttaaaGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCNGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTaca                                                                                                                                                                                                                    <  2:749932/298‑1 (MQ=255)                                                                  tGAAAGGCCCGCAAGTAGCCTAGCAGCCTGCAGCCGTTCCGTGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTaca                                                                                                                                                                                                                    <  1:279168/300‑1 (MQ=255)                                                                  tGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTaca                                                                                                                                                                                                                    <  2:479595/300‑1 (MQ=255)                                                                                           gcCTGCAGCCGTTCCGGGCACCTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCNTACGGCTCTATGGCAACGACTCGCTCNATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTACGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCtt                                                                                                                                                                                           >  2:569999/1‑300 (MQ=255)                                                                                           gcCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGATCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCCACGAATCGCTCAATTCGACCTTCACCCCATCGACCGTGGCGCGCCGATGAAAGCACTTCGCCTTCCGGGCAGAAACGGGGATGGAGTTCTCGCCGGTGCACTTCTNGCCGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAAATGGAGATACTGCtt                                                                                                                                                                                           >  2:641569/1‑300 (MQ=255)                                                                                           gcCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCtt                                                                                                                                                                                           <  1:705278/300‑1 (MQ=255)                                                                                           gcCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGAACCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGTGCCCGGGCTTCNTACGGCTCTATGGCAACGACTCGCTCNATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTAATGCtt                                                                                                                                                                                           >  2:572361/1‑300 (MQ=255)                                                                                               gCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTtgtg                                                                                                                                                                                       >  1:106571/1‑300 (MQ=255)                                                                                                  gCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGAc                                                                                                                                                                                    <  1:367585/300‑1 (MQ=255)                                                                                                          gggCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgagga                                                                                                                                                                            <  1:682522/300‑1 (MQ=255)                                                                                                            gCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGaggagg                                                                                                                                                                          <  1:759288/300‑1 (MQ=255)                                                                                                               aCTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGAc                                                                                                                                                                       <  1:395681/300‑1 (MQ=255)                                                                                                                         cGATTTCGACGCCAGTTCGCTTGACCCTGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAAc                                                                                                                                                             <  2:326849/300‑1 (MQ=255)                                                                                                                         cGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACTGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAAc                                                                                                                                                             <  2:545796/300‑1 (MQ=255)                                                                                                                         cGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAAc                                                                                                                                                             <  1:89548/300‑1 (MQ=255)                                                                                                                         cGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAAc                                                                                                                                                             <  2:342991/300‑1 (MQ=255)                                                                                                                                gACGCCGGTTCACTTGACCCGGAGCTGCATACCCTGCGCATTCCGTTCGACAACACCCTCTGCTCGCTCACCGCGCGCCCGGTCTCCTTACTGCTCTATGGCAACTACTCGCTCAATTCGACCTGCACCCAATCGACCGGGGCGCGCCGCTGACAACACTTCGCCTTCCGGGCAGAAACGCAGATGGAGTTCTCACCGGTGCACTGCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGTAAAAACTGGAGCTACTGCTTTGTGGGCTACGAGGAGGGACAGGTTAGAACCATCaaa                                                                                                                                                      <  2:112900/300‑1 (MQ=255)                                                                                                                                  cGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGt                                                                                                                                                    >  1:100553/1‑300 (MQ=255)                                                                                                                                       gTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATcc                                                                                                                                               <  1:369345/300‑1 (MQ=255)                                                                                                                                               gACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAAcctgtctcttatacacatctcgc                                                                                                                                       <  1:293727/300‑22 (MQ=255)                                                                                                                                                aCCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGAcc                                                                                                                                      >  1:158560/1‑300 (MQ=255)                                                                                                                                                       gCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGCCTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGTACTTCGCCTTACGGGCAGAAACGCGGATGGAGTTCTCGACGGTGCACTTCCCACAGAGCGCGGGGCTGACCTGCTCCTCCAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTCCGAGGAGGGACAGGGTCGAACCATCAAAGTTATCCAGATCGACCACAACGt                                                                                                                               >  2:122893/1‑300 (MQ=255)                                                                                                                                                        cTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTAAACCAATCGACCGTGGGGCGCCGCTGACAGCACTTAGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACcaaaaagtg                                                                                                                              >  2:534525/1‑293 (MQ=255)                                                                                                                                                          gCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTNGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAACAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGATCGACCACAACGTGcc                                                                                                                            >  2:203822/1‑300 (MQ=255)                                                                                                                                                          acAGACCCTGCGCATTTCGTTCTACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATTGCAACGACTCGCTCAACTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGTAGAAACGCGGATTGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGTAGGGACAGGGTAGAACTATCAAAGTTATCCAGCTCGACCACAACGTGcc                                                                                                                            <  2:438659/299‑1 (MQ=255)                                                                                                                                                              aCCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGCCTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCcgtcg                                                                                                                        >  1:812852/1‑300 (MQ=255)                                                                                                                                                                 cTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTgg                                                                                                                     >  1:821423/1‑300 (MQ=255)                                                                                                                                                                   gcgcATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGcc                                                                                                                   <  1:167881/300‑1 (MQ=255)                                                                                                                                                                                 acgacACCCTCGGCTCGCTCACCGCGCGCCCNGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCNGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGAACACAACGTGCCGTCGTGGCCGCTGCACGagcagc                                                                                                     >  2:597088/1‑300 (MQ=255)                                                                                                                                                                                 acgacACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCATCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGagcagc                                                                                                     >  2:500405/1‑300 (MQ=255)                                                                                                                                                                                        ccgcGGCTCGCTTACCGCGCGCCCGGGCTTCCTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTcc                                                                                              <  2:81548/297‑1 (MQ=255)                                                                                                                                                                                        ccTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCATCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTcc                                                                                              <  1:509278/300‑1 (MQ=255)                                                                                                                                                                                                cgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGAACTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAAAAACGCGGATGGAGTTCTCGCAGGTGCACTTCCAGCAGAGCGCGGAGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGTTTTGTGGACTACGAGGGGGAACGGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTACCGTTGCGGCCGCCGCCCGAGCAACCCACTCCGGTGTCgg                                                                                      >  2:93884/1‑300 (MQ=255)                                                                                                                                                                                                cactcACCGCGCGCCCAAGCCTCCCACGACTCTATGGCAACGACCCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGACGGTGCACTTCCAGCAGAGCGCAGGACTGACCTGCTACTACAACAGCAAAACCTGGAGCTACTGCTTTGTGGACCACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCATCGTGGCCGCTGCACGAGCAGCCCATCCCGGTGcaga                                                                                      >  2:397468/3‑297 (MQ=255)                                                                                                                                                                                                  ctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGaa                                                                                    >  1:320713/1‑300 (MQ=255)                                                                                                                                                                                                  ctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCACATTCCGGTGCCGGaa                                                                                    >  2:818267/1‑300 (MQ=255)                                                                                                                                                                                                      ccGCGTGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAATGCGGATGGAGTTCTCGCCGGTGCCCTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAGAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATg                                                                                >  2:801272/1‑300 (MQ=255)                                                                                                                                                                                                           cncaagggaggaggaaggCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCATCCCATTCCGGTGCCGGAACATGCGgag                                                                           >  1:65814/17‑300 (MQ=255)                                                                                                                                                                                                               cGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCg                                                                       <  1:429800/300‑1 (MQ=255)                                                                                                                                                                                                                     tcttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCAGGTGCCGGAACATGCGGAGAGCGTCTGGc                                                                 >  1:425007/1‑300 (MQ=255)                                                                                                                                                                                                                            gCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGTACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGAAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACACCGTGCCGTCGTGGCCGCTGCACGAGTAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCgggc                                                          >  2:293257/1‑299 (MQ=255)                                                                                                                                                                                                                               ctATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCAGGAACATACGGAGAGCGTCTGGCTGCGGGTGGa                                                       >  1:150680/1‑300 (MQ=255)                                                                                                                                                                                                                                     cAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGNAGGGACAGGGTAGAACCATCAAAGTT‑TCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGNGGAGAGCGTCTGGCTGCGGGTTGACGTGGAt                                                <  2:618102/300‑1 (MQ=255)                                                                                                                                                                                                                                                   aTTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGNCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGNTCTAcc                                   >  2:698237/1‑300 (MQ=255)                                                                                                                                                                                                                                                            tCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCt                          >  1:263414/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        ccGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGTGCAGAAACGCGGATGGAGTTCTCGCCGGGGCACTTCCAGCAGAGCGTGGGGCTGACCGGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGGGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCTGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCACTACAGCTACTCGTTGGATg              <  2:249317/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        ccGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATg              <  1:679187/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          gTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATGGc            >  1:238146/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gcTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGc  >  1:446829/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        CGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGC  >  NC_000913/287667‑288241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑