Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,324	G→A	G179D (GGC→GAC)	yagH →	putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,324	0	G	A	100.0%	169.0 / NA	52	G179D (GGC→GAC)	yagH	putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/31);  total (21/31)

GGTAAATTCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCG  >  NC_000913/287032‑287588                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                         ggtcaaTTCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCAACGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCATGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCAGCGACACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTAGCCCGAGCGCAAAGAGCCGCTTACCGACAcgccg                                                                                                                                                                                                                                                                   >  2:39146/5‑300 (MQ=255)    aagtttcggctacttgacacCGACGGGAAGATTTTTAACTCACCGTGGAAAAACGGCCGCAACTTTCTCCTCACCGCGCCCCGCGTCGAGGGGCCGTGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAAGACTATATTTACTACCCTTGGGGGCCGCGCCACCAAAGCAACCCACACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACTGGCACGCTCTTGCCCGAGAGAAAAACGCTGTTGACCGACAcgccgcgc                                                                                                                                                                                                                                                                <  2:415608/284‑3 (MQ=255)       ttCTGGTTACTCGACACCGACGGGAAGATTGTCGACTCTCCGTGGAAAAACGACCAAAACTTCCTCGTCACCACACCCCCCACCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGTCCGCAAATACTATATCTACCACCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACGGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGc                                                                                                                                                                                                                                                             <  2:302995/300‑1 (MQ=255)       ttCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGACAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGc                                                                                                                                                                                                                                                             <  2:551882/300‑1 (MQ=255)            gctgctCGACACCAACGGGGAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTCCCTCGTCACCTCACCCTCCATCGAGGTGCCAGGTAGCGAGCCAACCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACNATGGCCGCAAATACTATATCTACCGCNCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGTGTTTGACCCGCAGACCGACACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCACTCTGCTacac                                                                                                                                                                                                                                                        <  2:578715/300‑1 (MQ=255)               gctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACNCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGNCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGa                                                                                                                                                                                                                                                     >  2:686699/1‑300 (MQ=255)               gctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGa                                                                                                                                                                                                                                                     >  1:24453/1‑300 (MQ=255)                ctctACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGaa                                                                                                                                                                                                                                                    >  1:519022/1‑300 (MQ=255)                                aGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGAAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACCGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACACGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTCCCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGtctc                                                                                                                                                                                                                                    >  2:323798/1‑297 (MQ=255)                                 gATTGTTGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCTTCACCGCGCCCTCCATCGAGGGGCCATGGAGCAAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCTTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCg                                                                                                                                                                                                                                   <  2:365538/300‑1 (MQ=255)                                  attttCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCNTCGAGGTGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGTCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGc                                                                                                                                                                                                                                  <  2:632587/296‑1 (MQ=255)                                                 tGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCTAGGTGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACCCTGTTTACCGACACGCCGCTCTGCTACACCTAAGACGCGCACCTGTATCGCCACGCGGGATGGtac                                                                                                                                                                                                                   <  2:448694/300‑1 (MQ=255)                                                 tGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCGTGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCATTACGACGATGGCCGCAAATACTATATCTACCGCCCTTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCACCGTGTTACAGGCGTTGGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGtac                                                                                                                                                                                                                   <  2:396135/300‑1 (MQ=255)                                                    aaaaaCGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGtactac                                                                                                                                                                                                                <  1:510633/300‑1 (MQ=255)                                                      aaaCGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTTTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCt                                                                                                                                                                                                              <  1:464/300‑1 (MQ=255)                                                      aaaCGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCt                                                                                                                                                                                                              <  1:19584/300‑1 (MQ=255)                                                      aaaCGGCCACAATTTCCTCGGAACTACGCCCGCCATCGAGGGGCCATGGAGCGAGCAAATCCCGATGGGAAACGGCGGGTTTGACCCGTCCCTGTTCCATGACGACGATGGCCGCAAAGACTATATCTACCACCCGTGGGGTCCGCGCAACCAAAACAACCCGCACACCACCATCTTGTTACAGGCGTTTGACCCGCAGACCGGCACGCCCTCGCCCGAGCGCAAAACGCTGTTTACTGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCt                                                                                                                                                                                                              <  2:99712/300‑1 (MQ=255)                                                      aaaCGACCGCAATTTCTTCGTCACCGCACCTTCTGTCTGGGGGCCATTGAGCGGGCCAATCCCGATGGGCAATGTCGGGTTTGACCCGTCCCTGTTCCACGACTACGATGGCTACAAATACTATACCTACCCCCCTCTGGGTCCGCTCCACCACAGCAACCCGCACACCACCATCTTGTTACATGCGTTTGACCCGCGGATCGGCACGCTTTTGCCTGGGCGCAAAGCGCTGTTTACTGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCt                                                                                                                                                                                                              <  2:135416/300‑1 (MQ=255)                                                                     ccTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCAACACAGCAACCAGCACAACACCATCGTGTTACCGTCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATAACCGCAGAggg                                                                                                                                                                                               >  2:69184/1‑300 (MQ=255)                                                                           cACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCAc                                                                                                                                                                                         <  1:416717/300‑1 (MQ=255)                                                                                 gcCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTa                                                                                                                                                                                   <  1:429806/300‑1 (MQ=255)                                                                                        aTCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCAc                                                                                                                                                                            >  1:395018/1‑300 (MQ=255)                                                                                                gCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGACACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCcgtcgt                                                                                                                                                                    >  1:509717/1‑300 (MQ=255)                                                                                                  cATGGAGCGACCCAATCCCGATGGGCAACGACGGGTTTGACCCGCCCCTGTTCCACGACGACGAGGGCCGCAAAGACTATATCTACCGCCCGTGGGGGCTGCGCCACCACAGCAACCCGCACAACACCGTCGTGTTACATGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCAAGCGCAAAACGCTGTGTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCGGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCgtgg                                                                                                                                                                  <  2:144019/300‑1 (MQ=255)                                                                                                     ggAGCGAGCCAATCCCGAGGGGCAACGGCGGGTTTGACCCGTCCCGGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGGGGGGGCCGCGCCACCACAGGAACCCGCACAACACCGTCGTGTTACAGTTGTTTGACCCGCAGACCGGCACGCCCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCTGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGtgc                                                                                                                                                               <  1:148470/300‑1 (MQ=255)                                                                                                                                   ggTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACg                                                                                                                                 >  1:634844/1‑300 (MQ=255)                                                                                                                                   ggTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCAAGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACg                                                                                                                                 >  1:143712/1‑300 (MQ=255)                                                                                                                                       tGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCTCCACCACAGCAACCCGCACAACACCACCTTGTTACAGGCGTTTGACCCGCAGACCGGCACACTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCt                                                                                                                             <  2:766959/300‑1 (MQ=255)                                                                                                                                            cGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCAcc                                                                                                                        <  1:325296/300‑1 (MQ=255)                                                                                                                                             gTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTCCGAGCAAGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCAccc                                                                                                                       >  2:18817/1‑300 (MQ=255)                                                                                                                                              tCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCg                                                                                                                      >  1:77254/1‑300 (MQ=255)                                                                                                                                              tCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCAGCACGCTCTCGCCCGAGCGCAAAACGATGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCAAGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGGGCTGCACCCg                                                                                                                      >  2:260379/1‑300 (MQ=255)                                                                                                                                                 cTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGAc                                                                                                                   >  1:81721/1‑300 (MQ=255)                                                                                                                                                        acgacgacgATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGTTCTCGCCCGAGCGTAAAATGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGAAGCGTACCTGTATTGCCACGCGGGATGGTACTAACTGATGGCCGACGAGGGCGGAACCAGCTACGGGCAAGCAGTCGTGATGCTGCGTTCCAAAAATATCGACGGGCAGTAagagttgtacatggaagtgacga                                                                                                            >  2:277252/1‑281 (MQ=255)                                                                                                                                                                gaTGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTGACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGAcc                                                                                                    <  1:523856/300‑1 (MQ=255)                                                                                                                                                                         aaatcccctatCTCCCGCCCTTGGGGGCCGCGCCACCACAGCAACCCGCACAACCCCATCGTGTTACAGGCGTTTGCCCCGCAGCCCTGCACGCTCTCACCCGAGCGCAAAACGCTGTCTACCAACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCTGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGTTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGGAACGACGATGACCAGCTGGCAc                                                                                           <  2:823121/292‑1 (MQ=255)                                                                                                                                                                                  atCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGNACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGgag                                                                                  >  2:742513/1‑300 (MQ=255)                                                                                                                                                                                       ccGCCCGGGGGGGCCGCTCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTGTGACACGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAAccc                                                                             <  1:326493/300‑1 (MQ=255)                                                                                                                                                                                               gggggCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCTGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCaga                                                                     <  1:682888/300‑1 (MQ=255)                                                                                                                                                                                                 gggCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCagaag                                                                   <  1:88903/300‑1 (MQ=255)                                                                                                                                                                                                  ggCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGCACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCagaaga                                                                  >  2:364182/1‑300 (MQ=255)                                                                                                                                                                                                               caGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTc                                                     >  1:766750/1‑300 (MQ=255)                                                                                                                                                                                                                      ccGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACNAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCNctgctg                                              >  2:689947/1‑300 (MQ=255)                                                                                                                                                                                                                         cacaACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAg                                           <  1:818783/300‑1 (MQ=255)                                                                                                                                                                                                                           caacaCCCTCGTGTGACAGTCGTTTGACCCACAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCTGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGAc                                         <  2:819309/300‑1 (MQ=255)                                                                                                                                                                                                                                     gtgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGt                               >  1:113346/1‑300 (MQ=255)                                                                                                                                                                                                                                      tgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCAACGCGGGGTGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTCCAAAAAATATCGAAGGGCCGTACGAGCTGCACCCGGAAGTAACGATAATGCCCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAAACGCATACGGGTg                              >  2:89240/1‑300 (MQ=255)                                                                                                                                                                                                                                              tcatttgacccacataccgccgcgcgcgctcccGAGCGCAAAACGCTGTGGACCGACACGCCGCTCTGCTACACCCAAGACGCGCACCTGTATCGCCACGCTGGATGGTATTACCGGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGACACCTGCCGGAAAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTAc                      <  2:256595/270‑1 (MQ=255)                                                                                                                                                                                                                                                   tGACCTGCAGACCGGCGCGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATTGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGc                 <  2:462694/300‑1 (MQ=255)                                                                                                                                                                                                                                                   cgACCCGCAGACCGGCACACTCGCTCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCTAAGACGCGCACCTGTATCGCCACGCTGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGc                 <  2:476325/299‑1 (MQ=255)                                                                                                                                                                                                                                                                ggcgcGCTCTCACCAGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATTGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGTTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAgc    <  2:303141/296‑1 (MQ=255)                                                                                                                                                                                                                                                                  cACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGCGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAgccg  <  2:511527/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                         GGTAAATTCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCG  >  NC_000913/287032‑287588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑