Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,848,669	C→T	L191L (CTG→CTA)	hycC ←	formate hydrogenlyase subunit HycC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,848,669	0	C	T	100.0%	203.3 / NA	59	L191L (CTG→CTA)	hycC	formate hydrogenlyase subunit HycC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/29);  total (30/29)

GGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGCAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCGTTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAG  >  NC_000913/2848383‑2848963                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                       ggCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCGCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATAc                                                                                                                                                                                                                                                                                           <  2:515576/300‑1 (MQ=255) ggCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATAc                                                                                                                                                                                                                                                                                           <  1:295393/300‑1 (MQ=255)  gcgcTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACg                                                                                                                                                                                                                                                                                          >  1:533540/1‑300 (MQ=255)       ggATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCatat                                                                                                                                                                                                                                                                                     >  1:15709/1‑300 (MQ=255)         aTGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCGGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATcc                                                                                                                                                                                                                                                                                   <  2:323944/300‑1 (MQ=255)         aTGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATcc                                                                                                                                                                                                                                                                                   >  1:547457/1‑300 (MQ=255)              gtgCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCcagcag                                                                                                                                                                                                                                                                              <  2:363450/300‑1 (MQ=255)                    caccaGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAg                                                                                                                                                                                                                                                                        <  2:256409/300‑1 (MQ=255)                       caGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATc                                                                                                                                                                                                                                                                     <  1:519880/300‑1 (MQ=255)                        aGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGTAGCGCGATCCCCCATCACAGCGGTGCATTACCGCCCAGCGGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATcc                                                                                                                                                                                                                                                                    <  2:59196/300‑1 (MQ=255)                        aGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATcc                                                                                                                                                                                                                                                                    >  1:534227/1‑300 (MQ=255)                                 aGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCg                                                                                                                                                                                                                                                           <  1:856210/300‑1 (MQ=255)                                  gACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCAGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGt                                                                                                                                                                                                                                                          >  2:131140/1‑300 (MQ=255)                                     caccGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCGGCAGTGACAGGGTTAAAATGCCCAGCAGTCCAATTTTCATGACTACCGTAGAAAACGACGCGGCAGCTGGCGCAGAGTCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCTGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATATGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAAc                                                                                                                                                                                                                                                       <  2:101842/300‑1 (MQ=255)                                     caccGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGGGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCTAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAAc                                                                                                                                                                                                                                                       <  2:101853/300‑1 (MQ=255)                                       ccGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGc                                                                                                                                                                                                                                                     <  1:668026/300‑1 (MQ=255)                                                                         gcgcGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCAGTAACGCTGCCACAGCAGCAAGCAAGCAACCGCcagcagta                                                                                                                                                                                                                   >  2:348847/1‑298 (MQ=255)                                                                          cgcgATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACGCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCcagcagcag                                                                                                                                                                                                                  <  1:202351/300‑1 (MQ=255)                                                                              aTCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTg                                                                                                                                                                                                              >  1:157516/1‑300 (MQ=255)                                                                                 ccccACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGNCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCNAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGccg                                                                                                                                                                                                           >  2:676600/1‑299 (MQ=255)                                                                                 ccccACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCCGCAGCAGAGTGCCa                                                                                                                                                                                                           >  1:399205/1‑300 (MQ=255)                                                                                    caccacAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCANCCAGCCGTGCAGCGGAATAATCCCGGNCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGa                                                                                                                                                                                                        >  2:572573/1‑300 (MQ=255)                                                                                    caccacAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCNGGCCAGCAGGCCAAAGCCAATCACTCNGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGa                                                                                                                                                                                                        <  2:584549/300‑1 (MQ=255)                                                                                    caccacAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGa                                                                                                                                                                                                        <  1:192109/300‑1 (MQ=255)                                                                                    caccacAGCGGTGCAGTACCGCCCAGCAGTGACAGGGGTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGa                                                                                                                                                                                                        <  2:30662/300‑1 (MQ=255)                                                                                          aGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGcccc                                                                                                                                                                                                  <  2:647469/300‑1 (MQ=255)                                                                                              gTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAgcg                                                                                                                                                                                              >  1:165274/1‑300 (MQ=255)                                                                                                gCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCa                                                                                                                                                                                            >  1:496229/1‑300 (MQ=255)                                                                                                                      gggTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCANCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCAGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCNCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTttgc                                                                                                                                                                      >  2:790316/1‑300 (MQ=255)                                                                                                                      gggTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTttgc                                                                                                                                                                      >  1:103376/1‑300 (MQ=255)                                                                                                                      gggTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCACTCTttgc                                                                                                                                                                      >  1:103522/1‑300 (MQ=255)                                                                                                                      gggTTAAAATGCCAAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCAGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGTGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCAAAGCAGCCAGCAAGCAATCGCCAGCAGCAGCGTGCCAAGACGCCCCAGAGCAAACCACAGTTTGCCCTCTttgc                                                                                                                                                                      >  1:103466/1‑300 (MQ=255)                                                                                                                                       agGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAac                                                                                                                                                     <  2:104178/300‑1 (MQ=255)                                                                                                                                         gCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAacac                                                                                                                                                   <  1:76110/300‑1 (MQ=255)                                                                                                                                                         aCCGTAGAAAACAACGCGGCAGCTGGCGAAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCAAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCACTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATg                                                                                                                                   >  2:648263/1‑300 (MQ=255)                                                                                                                                                                                  gcgcAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGNTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGNACATGc                                                                                                          >  2:696451/1‑300 (MQ=255)                                                                                                                                                                                  gcgcAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGc                                                                                                          >  1:812928/1‑300 (MQ=255)                                                                                                                                                                                  gcgcAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCAAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCAAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTAACACGAACATGc                                                                                                          >  1:112671/1‑300 (MQ=255)                                                                                                                                                                                                gCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCa                                                                                            >  1:823502/1‑300 (MQ=255)                                                                                                                                                                                                     tgCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCTGCCAGCAGGCCAAAGCCGATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGAc                                                                                       <  2:396058/300‑1 (MQ=255)                                                                                                                                                                                                     tgCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGAc                                                                                       <  1:120417/300‑1 (MQ=255)                                                                                                                                                                                                      gCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGaag                                                                                      >  1:33365/1‑298 (MQ=255)                                                                                                                                                                                                      gCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACg                                                                                      >  1:496249/1‑300 (MQ=255)                                                                                                                                                                                                       ccTGCGGCGCCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGTTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACgg                                                                                     <  2:144618/300‑1 (MQ=255)                                                                                                                                                                                                             gCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGTAGGCCAAAGCAAATCACTCCGAGCAGCCAGGTATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCAAGCAGCAGAGTGCAAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTCGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCACGATTTCGACCATTACCACGAACATGCCGAGGTTGTTGGCAATGGCGGCGCAGa                                                                               >  2:34773/1‑300 (MQ=255)                                                                                                                                                                                                                  cAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGAcggcg                                                                          >  1:314161/1‑300 (MQ=255)                                                                                                                                                                                                                    gCCGTGTAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCATTCGCCAGCAGCAGAGTGCCAAGACGCNCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCGGTTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGNGGTTGCGGGCAATGACGGCGCAggcggcggc                                                                        <  2:591643/300‑1 (MQ=255)                                                                                                                                                                                                                    gCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACggcggc                                                                        >  1:673307/1‑300 (MQ=255)                                                                                                                                                                                                                      cGTGTAGCGGAATAATCCCTGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAACAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCTGCCATTACCACAAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAg                                                                      <  2:153188/300‑1 (MQ=255)                                                                                                                                                                                                                       gTGCAGCGGAATAATCCAGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGTCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCAAAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCAAAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTAACAAGAACATGACGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGc                                                                     >  2:72709/1‑300 (MQ=255)                                                                                                                                                                                                                               gAATAATCCAGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGTAGccgccaaca                                                             >  2:289705/1‑293 (MQ=255)                                                                                                                                                                                                                                     tCCCGTCCAGCAGGCCAAAGCCAACCACTCCGAGCAGCCAGAGATCGGAACCAAGCGGTAGCTGTTGCATACGTATAGCCAGCAGGCACAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTTAGGAACACCGCGCGCAGGGCCATGATTTCGGCCATTACCACGAACATTCCGAGGTTGCTGGCAATGACGGCGCAGACTGCGGCAGCCATCAACATATTGa                                                       <  2:71462/300‑1 (MQ=255)                                                                                                                                                                                                                                     tCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATACAGCATGCACAGATCCAGCGTGCCTTAACGCTGCCACAGCAGCCAGCAAGCAGTCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGa                                                       <  2:71133/300‑1 (MQ=255)                                                                                                                                                                                                                                     cccctgCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACACTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGa                                                       <  2:71020/295‑1 (MQ=255)                                                                                                                                                                                                                                                 ggCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCGt                                           >  1:807862/1‑300 (MQ=255)                                                                                                                                                                                                                                                          cAATCACTCCGAGCAGCAAGATATCGGAACCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGNTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATACCGAGGTTGCTGGCAATGCCGGCGCAGACGGCGGCAGCCATCAACATCTTGATCTGCAAGCCGTTGCGCttaa                                  >  2:215434/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                     aaCCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGTCGCAGATCCAGCATGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCACGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACTGCGCAAACTGCGGCAGCCATCAACATATTGATCTGCAAGCCGTTGCACTTCACCTGCGCGTGGCGATGCCAGTTAAtgt       <  2:165184/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     aaCCGAGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCGTTGCACTTCACCTGCGCGTGGCGATGCCAGTCAAtgt       <  2:279696/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          aGCGGTAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCGTTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTag  <  1:48657/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                       GGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTAAAATGCCCAGCAGGCCAATTTTCATGACTACCGTAGAAAACAACGCGGCAGCTGGCGCAGAGGCGTTCGCATGTGCCTGCGGCACCCAGCCGTGCAGCGGAATAATCCCGGCCAGCAGGCCAAAGCCAATCACTCCGAGCAGCCAGATATCGGAACCGAGCGGCAGCTGTTGCATACGCATATCCAGCAGGCGCAGATCCAGCGTGCCGTAACGCTGCCACAGCAGCCAGCAAGCAATCGCCAGCAGCAGAGTGCCAAGACGCCCCAGCGCAAACCACAGTTTGCCCTCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCGTTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAG  >  NC_000913/2848383‑2848963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑