Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,464,645	C→T	Q59* (CAG→TAG)	paaY →	2‑hydroxycyclohepta‑1,4,6‑triene‑1‑carboxyl‑CoA thioesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,464,645	0	C	T	100.0%	279.2 / NA	84	Q59* (CAG→TAG)	paaY	2‑hydroxycyclohepta‑1,4,6‑triene‑1‑carboxyl‑CoA thioesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/61);  total (23/61)

TAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTCAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCA  >  NC_000913/1464350‑1464940                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                         tAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGa                                                                                                                                                                                                                                                                                                      <  2:806904/300‑1 (MQ=255)          ctgctTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGACCCGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATCTAGGATAATTGCGt                                                                                                                                                                                                                                                                                             >  2:455188/1‑300 (MQ=255)          ctgctTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGt                                                                                                                                                                                                                                                                                             <  2:391942/300‑1 (MQ=255)            gctTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTa                                                                                                                                                                                                                                                                                           >  2:402531/1‑300 (MQ=255)            gctTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTa                                                                                                                                                                                                                                                                                           >  1:190498/1‑300 (MQ=255)                 aGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCAc                                                                                                                                                                                                                                                                                      >  1:542290/1‑300 (MQ=255)                    gttcgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCAAACATTTAGGATAATTGCGTTATGCACGGt                                                                                                                                                                                                                                                                                   <  1:74329/300‑1 (MQ=255)                    gttcgtnAGTGAAAAAGGTGAAACCTCGTTCGGTGAACTGCCTGCGCCGGGAAGNCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGt                                                                                                                                                                                                                                                                                   <  2:704327/300‑1 (MQ=255)                      tcgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGttt                                                                                                                                                                                                                                                                                 >  1:183811/1‑300 (MQ=255)                                     ttGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGTCTTGAATATTGAACAGGAGGTGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAGCATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATAc                                                                                                                                                                                                                                                                  <  2:107164/299‑1 (MQ=255)                                     gTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATAc                                                                                                                                                                                                                                                                  <  1:520642/300‑1 (MQ=255)                                        aaaCCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGTCGGCTTGAATATTGAACAGTAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGTGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTTGCGATTTTGGTCGTATCTTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACt‑gt                                                                                                                                                                                                                                                              <  1:273207/300‑1 (MQ=255)                                        aaaCCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGNCTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACt‑gt                                                                                                                                                                                                                                                              <  1:579397/300‑1 (MQ=255)                                        aaaCCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACt‑gt                                                                                                                                                                                                                                                              <  1:73098/300‑1 (MQ=255)                                         aaCCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGCTTTTGGTCGTATCGTGGTGAAAGATGGNGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACt‑gtt                                                                                                                                                                                                                                                             >  2:630460/1‑300 (MQ=255)                                              cggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAgg                                                                                                                                                                                                                                                        <  1:403095/300‑1 (MQ=255)                                              cggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAgg                                                                                                                                                                                                                                                        <  2:508216/300‑1 (MQ=255)                                               ggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGga                                                                                                                                                                                                                                                       <  1:720609/300‑1 (MQ=255)                                                      gAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCNAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATg                                                                                                                                                                                                                                                >  1:603301/1‑300 (MQ=255)                                                          tgcctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACa                                                                                                                                                                                                                                            <  1:446255/300‑1 (MQ=255)                                                              tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGTGCGTTTACGTTGGGCNAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCNGGATACT‑GTTGTAGTAGAAGATGGACATAtt                                                                                                                                                                                                                                        <  2:614868/300‑1 (MQ=255)                                                                       aaGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCNTCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAgc                                                                                                                                                                                                                               <  1:590791/300‑1 (MQ=255)                                                                       aaGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAgc                                                                                                                                                                                                                               <  2:43577/300‑1 (MQ=255)                                                                       aaGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAgc                                                                                                                                                                                                                               <  1:683604/300‑1 (MQ=255)                                                                            tGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTAt                                                                                                                                                                                                                          >  2:282181/1‑300 (MQ=255)                                                                              tATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATcc                                                                                                                                                                                                                        <  1:156975/300‑1 (MQ=255)                                                                              tATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTNTACGTTGGGCCAAATGCCAGCCTGCGNGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATcc                                                                                                                                                                                                                        <  2:577943/300‑1 (MQ=255)                                                                              tATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTAGGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATcc                                                                                                                                                                                                                        <  1:80882/300‑1 (MQ=255)                                                                              tATTTTCAACGTTTTGGCGGCTTGAATATGGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATcc                                                                                                                                                                                                                        <  1:747148/300‑1 (MQ=255)                                                                                   tCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATCCTTCAc                                                                                                                                                                                                                   <  1:544791/300‑1 (MQ=255)                                                                                   tCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGNATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCAc                                                                                                                                                                                                                   <  1:627357/300‑1 (MQ=255)                                                                                      aCGTTTTGTCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGc                                                                                                                                                                                                                <  1:200336/300‑1 (MQ=255)                                                                                                            gAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGCATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGTAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAACGCAt                                                                                                                                                                                          >  2:410679/1‑300 (MQ=255)                                                                                                                 ggaggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCAAGGCTGCATTATCCGCCGCAATGCATTAGTg                                                                                                                                                                                     <  1:439884/300‑1 (MQ=255)                                                                                                                          aTGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCTAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTNGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAAc                                                                                                                                                                            <  2:189342/300‑1 (MQ=255)                                                                                                                                         aTAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGAc                                                                                                                                                             <  2:456602/300‑1 (MQ=255)                                                                                                                                         aTAGACGGCCTGACTCCGGTTGTGCCATAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAAGGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATGCT‑GTTGTAGTAGAAGATGTACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGAc                                                                                                                                                             <  2:233076/300‑1 (MQ=255)                                                                                                                                           aGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGAcgg                                                                                                                                                           <  1:144008/300‑1 (MQ=255)                                                                                                                                                gTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGAcggtgcgg                                                                                                                                                      >  1:123466/1‑300 (MQ=255)                                                                                                                                                gTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGANACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGAcggtgcgg                                                                                                                                                      >  1:598278/1‑300 (MQ=255)                                                                                                                                                   tggcTCCGGTTGTGCCAGAAGAGAGTTTTGCCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCTAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGa                                                                                                                                                   <  2:2931/297‑1 (MQ=255)                                                                                                                                                   tGACTCCGGTTGTGTCATAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATACTTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGa                                                                                                                                                   <  2:288551/300‑1 (MQ=255)                                                                                                                                                   tGACTCCGGTTGTGCCAGAATAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGa                                                                                                                                                   <  2:32509/300‑1 (MQ=255)                                                                                                                                                   tGACTCCGGTTGTGCCAGAATAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGa                                                                                                                                                   <  2:536899/300‑1 (MQ=255)                                                                                                                                                   tGACTCCGGTTGTGCCAGAAGAGATTTTTGTCCATCCGACAGCGGTAGTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGGTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGa                                                                                                                                                   <  2:409956/300‑1 (MQ=255)                                                                                                                                                   tGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTAGGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGa                                                                                                                                                   <  2:516418/300‑1 (MQ=255)                                                                                                                                                   tGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTNCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGNATGAACGCGGTAGTGATGGACGGTGCGGTGa                                                                                                                                                   <  2:619137/300‑1 (MQ=255)                                                                                                                                                    gACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGAt                                                                                                                                                  >  2:811759/1‑300 (MQ=255)                                                                                                                                                      cTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGGTTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACCTATTGGTCATAGTGCTATTCTTCACGACTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGAAGGTGCGGTGATTg                                                                                                                                                >  2:4053/1‑300 (MQ=255)                                                                                                                                                       tCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTgg                                                                                                                                               <  1:484188/300‑1 (MQ=255)                                                                                                                                                          ggTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCNCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCga                                                                                                                                            >  2:624566/1‑300 (MQ=255)                                                                                                                                                          ggTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTGGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCga                                                                                                                                            <  1:780910/300‑1 (MQ=255)                                                                                                                                                              gtgCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAAc                                                                                                                                        <  1:103570/300‑1 (MQ=255)                                                                                                                                                               tgCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACa                                                                                                                                       >  2:224614/1‑300 (MQ=255)                                                                                                                                                               tgCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGAAGGTGCGGTGATTGGCGAGAACa                                                                                                                                       >  2:441647/1‑300 (MQ=255)                                                                                                                                                                   agaagaGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCAt                                                                                                                                   <  1:525040/300‑1 (MQ=255)                                                                                                                                                                           gTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTg                                                                                                                           <  1:225864/300‑1 (MQ=255)                                                                                                                                                                           gTTTTGCCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGCTTTCCCGAGCAGGATACT‑GTTGTAGTNGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGNGCGGTGATTGGCGAGAACAGCATTGTTGGTg                                                                                                                           <  2:594285/300‑1 (MQ=255)                                                                                                                                                                                         aCAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATACCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTtgtg                                                                                                             <  2:138929/300‑1 (MQ=255)                                                                                                                                                                                            gCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGaaa                                                                                                          >  1:249065/1‑300 (MQ=255)                                                                                                                                                                                                             tGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGcc                                                                                         <  1:529616/300‑1 (MQ=255)                                                                                                                                                                                                                      cGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTa                                                                                >  1:408773/1‑300 (MQ=255)                                                                                                                                                                                                                              gCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTg                                                                        <  1:768064/300‑1 (MQ=255)                                                                                                                                                                                                                                   tgcGTTGGGCCAAGTGCCAGCCTGCGTGTCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGc                                                                   <  2:36866/298‑1 (MQ=255)                                                                                                                                                                                                                                   tACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTANCCTTCACGGCTGCATTATCCGCCGCANTGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGc                                                                   <  2:584819/300‑1 (MQ=255)                                                                                                                                                                                                                                   tACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTTTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGc                                                                   <  1:767271/300‑1 (MQ=255)                                                                                                                                                                                                                                   tACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTctgc                                                                   <  2:228901/300‑4 (MQ=255)                                                                                                                                                                                                                                   tACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGc                                                                   <  1:509409/300‑1 (MQ=255)                                                                                                                                                                                                                                   tACGTTGGGCCAAATGCCAGCCGGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGc                                                                   <  2:95564/300‑1 (MQ=255)                                                                                                                                                                                                                                          ggCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCgg                                                            >  2:481286/1‑300 (MQ=255)                                                                                                                                                                                                                                                aTGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGNCATAGCGCTATCCTTCACGGCTGCATGATCCGCCGCAATGCATTAGTGGGAATGAACGCGGNAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCGAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAg                                                      >  2:686192/1‑300 (MQ=255)                                                                                                                                                                                                                                                              ggCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTc                                        <  1:73718/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      tGGTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCa                                <  1:407096/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        gTCGTATCGTGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAgg                              <  1:718196/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          catATCGTGGTGAAAGATGGCGCGAACATGTAGGATAATTGCGTTATGCACGGTTTTCCCAAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGTTCATAGCGCTATCCTNCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAAGGAACTCGGTAGTGATGGACGGTGNGGTGATTGTCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAg                            <  2:687325/298‑1 (MQ=255)                                                                                                                                                                                                                                                                          cGTATCGTGGGGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑ATTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTNCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGNGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAg                            <  2:687115/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          cGTATCGGGGTGAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGNGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGNGAAAGCGATTCGTGAACTCAGTGAGCAGGAg                            <  2:605328/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gtggtgAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCa                      <  2:499483/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 ggaggAAAGATGGCGCGAACGTTTAGGATAATGGTGTTATACACGGTTTTCCCGAGCAGGATACTTGTT‑TAGGAGAAGATGTACATATGGGTCATAGCGCTATCCTTAACGGCGGCATTATCCGCCGCAACGCATTAGTTGGAATGAACGCGGTAGTGATGGACGGTGCGGTGAGTTGCGAAAACAGCATTGTTGGTGCATCCGCATTTGCGAAAGCCAAAGCAGAAATGTCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGCGATCAGGAGTTggtag                     <  2:41913/296‑4 (MQ=255)                                                                                                                                                                                                                                                                                   gtgAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATgg                   >  2:58808/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   gtgAAAGATGGCGCGAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATgg                   >  1:178818/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 gAACATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGGTGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATGGAAAAAGCAGGGTAc     <  1:713983/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    cATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCa  <  1:284178/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    cATTTAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCa  >  2:158243/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                         TAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGTGAAAGATGGCGCGAACATTCAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACT‑GTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCA  >  NC_000913/1464350‑1464940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑