Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,523,244	C→T	G284D (GGC→GAC)	yjhG ←	D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,523,244	0	C	T	98.8%	271.4 / NA	83	G284D (GGC→GAC)	yjhG	D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (38/44);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGG  >  NC_000913/4522954‑4523523                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                         cTTCATTCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCNTCAACGGTCGGGATATGGCAACCTGCNTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTGTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGAc                                                                                                                                                                                                                                                                                 <  2:456050/300‑1 (MQ=255) cTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCNTCAACGGTCGGGATATGGCAACCTGCNTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGAc                                                                                                                                                                                                                                                                                 <  2:456244/300‑1 (MQ=255)     aTGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAg                                                                                                                                                                                                                                                                             >  1:287542/1‑300 (MQ=255)         aacaaTCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTTACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCTGGATGTGTAGCAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTGTTTGACTCAGGTTc                                                                                                                                                                                                                                                                         <  2:4228/300‑1 (MQ=255)             aTCCGANGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGNATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGGCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGAACGTCATCGCAGTCTCTATCGCTGTATCGGTGAGAATTTCCCGGTTGGTGATGTCTTTTTGACTCAGGTGCAgcg                                                                                                                                                                                                                                                                     <  2:576925/300‑1 (MQ=255)             aTCCGAGGCTGCGCAGATGCAACCTTACTTCCTGCACACCACCTACCATAAACGCATTGACCGTTGGATGATAAACCGGGCCAGTAGGCAGTACGCTCACCATTCGGGACACGCGCTTGTTGCTGCGAATCCATTCATCAACGTTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGTTGATGTCTTTTTGACTCAGGTTCAgcg                                                                                                                                                                                                                                                                     <  2:99029/300‑1 (MQ=255)             aTCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAgcg                                                                                                                                                                                                                                                                     >  2:631567/1‑300 (MQ=255)             aTCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAgcg                                                                                                                                                                                                                                                                     >  1:545289/1‑300 (MQ=255)              tcctaGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGTTGATGTCTTTTTGACTCAGGTTCAgcgc                                                                                                                                                                                                                                                                    <  2:313149/296‑1 (MQ=255)              tCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAgcgc                                                                                                                                                                                                                                                                    <  1:630801/300‑1 (MQ=255)                        cgcAGATGCAACATGACTTCCTGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGNTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTcgcgcg                                                                                                                                                                                                                                                          <  2:163414/300‑1 (MQ=255)                          cAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGTGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATTGCAACCTGCCTGGTGAGCAATTGCCTGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGTTGTTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGa                                                                                                                                                                                                                                                        <  1:702627/300‑1 (MQ=255)                            gATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAg                                                                                                                                                                                                                                                      >  2:340166/1‑300 (MQ=255)                            gATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAg                                                                                                                                                                                                                                                      >  1:235785/1‑300 (MQ=255)                             aTGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCTGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCTGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGc                                                                                                                                                                                                                                                     <  1:315850/300‑1 (MQ=255)                               gCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGctc                                                                                                                                                                                                                                                   <  1:582296/300‑1 (MQ=255)                                  aCATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCAGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAACTCTgg                                                                                                                                                                                                                                                >  2:144959/1‑300 (MQ=255)                                    aTGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGTTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCg                                                                                                                                                                                                                                              <  1:426749/300‑1 (MQ=255)                                                     accTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAgg                                                                                                                                                                                                                             <  1:57483/300‑1 (MQ=255)                                                       cTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCt                                                                                                                                                                                                                           <  1:633304/300‑1 (MQ=255)                                                         gCCATAAAGGCATTGACCGTTGGATGATAAANCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTNAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCAGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGAGCTCCCGCCACACAGGCTCa                                                                                                                                                                                                                         >  2:487673/1‑300 (MQ=255)                                                         gCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGACCCACCGCACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCa                                                                                                                                                                                                                         >  1:300359/1‑300 (MQ=255)                                                         gCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCCCCGAACGCGGCATGGACCGTCATCGCAGTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCACGGAAGCTCTAGCGATCTCCCGCCACACAGGCTCa                                                                                                                                                                                                                         >  2:76664/1‑300 (MQ=255)                                                         gCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCa                                                                                                                                                                                                                         >  1:92538/1‑300 (MQ=255)                                                                  gCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCCGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAggg                                                                                                                                                                                                                <  2:435817/300‑1 (MQ=255)                                                                  gCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAggg                                                                                                                                                                                                                <  1:220007/300‑1 (MQ=255)                                                                         ccGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATNTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGNTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAggg                                                                                                                                                                                                         >  2:563264/1‑300 (MQ=255)                                                                          cGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGc                                                                                                                                                                                                        <  1:159731/300‑1 (MQ=255)                                                                          cGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGc                                                                                                                                                                                                        <  2:431153/300‑1 (MQ=255)                                                                               gctgatAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAAt                                                                                                                                                                                                   >  1:107258/3‑300 (MQ=255)                                                                                atgatAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGACAGGGATGAAtg                                                                                                                                                                                                  >  1:392122/1‑300 (MQ=255)                                                                                          gggCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATNGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACNGGAAGGGGCCAGGGCTGAATGTGGGATTGcc                                                                                                                                                                                        <  2:488333/300‑1 (MQ=255)                                                                                             ccATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGt                                                                                                                                                                                     >  1:169632/1‑300 (MQ=255)                                                                                             ccATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCAAGGGCTGAATGTGGGATTGCCAGt                                                                                                                                                                                     >  2:225242/1‑300 (MQ=255)                                                                                             ccATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCAGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGCAGCTCTGGCGATCTCACGCCACACAGACTCACCGGAAGGGGACAGGGCTGAATGTGGGATTGCCAGt                                                                                                                                                                                     >  2:136280/1‑300 (MQ=255)                                                                                             ccATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCAGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGt                                                                                                                                                                                     >  1:193563/1‑300 (MQ=255)                                                                                             ccATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCATGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACACGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCAGGGTGGTGATGTCTTTTTGACTAAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCAGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGt                                                                                                                                                                                     >  2:373348/1‑300 (MQ=255)                                                                                                       gTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCtt                                                                                                                                                                           >  1:51563/1‑300 (MQ=255)                                                                                                         aCGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑g                                                                                                                                                                        >  1:649947/1‑300 (MQ=255)                                                                                                           gCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCAGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGc                                                                                                                                                                      >  2:446134/1‑300 (MQ=255)                                                                                                                ccAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGccacca                                                                                                                                                                 >  2:627366/1‑300 (MQ=255)                                                                                                                  attctgtgCACGCGCTTGGTGATGCGGATCCAGTCATCAACAGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAATGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCaccacc                                                                                                                                                               <  2:12749/293‑1 (MQ=255)                                                                                                                     ctgcgagatgtgtataagagacagATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGGATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGACACCACCTga                                                                                                                                                            >  1:103292/24‑300 (MQ=255)                                                                                                                      ggggCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTgag                                                                                                                                                           <  1:265248/300‑1 (MQ=255)                                                                                                                         gCACGTGCTTGTTGATGCGGATCCAGTAATCAACGGTCGGGATATGGCAACCTGNCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACTCGGCATGGACCGTCATCGCATTCTGTATCACTTTATCGGTGAGAATTTCCCGGGTGTTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGTCGATTTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGACTCCCAGTcttgagtgatatggcaacaactgagatg                                                                                                                                                         <  2:514097/300‑28 (MQ=255)                                                                                                                         gCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGTAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTAAATGTGGGATTGCCAGTcttgaatggtatggcgacaaccgagatg                                                                                                                                                         <  2:179882/300‑28 (MQ=255)                                                                                                                              cgcTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCAAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTACCg                                                                                                                                                   >  2:213429/1‑300 (MQ=255)                                                                                                                              cgcTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCNTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTccag                                                                                                                                                   >  2:155654/1‑298 (MQ=255)                                                                                                                                  tgttgATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGNGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAg                                                                                                                                               >  1:474930/1‑300 (MQ=255)                                                                                                                                  tgttgATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAg                                                                                                                                               <  1:89380/300‑1 (MQ=255)                                                                                                                                                          cGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAgccgccgc                                                                                                                       >  2:420733/1‑300 (MQ=255)                                                                                                                                                                ggATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAag                                                                                                                 >  1:620215/1‑300 (MQ=255)                                                                                                                                                                ggATATGGCAACCTGCCTGGTGAGCAATTGCAGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAag                                                                                                                 >  1:26985/1‑300 (MQ=255)                                                                                                                                                                           cctgcctgGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATNTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCAGGAAGAGGCACAGGcg                                                                                                      >  2:170765/1‑299 (MQ=255)                                                                                                                                                                           cctgcctgGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCAGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCt                                                                                                      >  1:277694/1‑300 (MQ=255)                                                                                                                                                                               cctgGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCAACACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTAc                                                                                                  <  2:427691/300‑1 (MQ=255)                                                                                                                                                                                 tgGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAg                                                                                                <  1:116300/300‑1 (MQ=255)                                                                                                                                                                                   gTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCACGGGTGGTGATGTCTTTCTGAATCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTatggcc                                                                                              >  2:183891/1‑295 (MQ=255)                                                                                                                                                                                            tGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGgcg                                                                                     <  1:338366/300‑1 (MQ=255)                                                                                                                                                                                                 ggATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCGTTTTGACTCAGGTTCAGCGCAGCTCGCGCGAAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACATCCGCCGCCGGAAGAGGCACAGGCTTTACAGACCGCACTgcgtgcct                                                                                >  2:94125/1‑298 (MQ=255)                                                                                                                                                                                                   aTGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTTAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTcc                                                                              <  1:261127/300‑1 (MQ=255)                                                                                                                                                                                                        tAACAGCAGTTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCNATCGCTTTATCGGTGAGAATTTCCCGGCTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGTAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑TGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTag                                                                         <  2:526897/300‑1 (MQ=255)                                                                                                                                                                                                            agcagGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGNCTCACCTGAAGGGGCCAGGGCTGAATNTGGGATTGCCAGTCCCAATCCTTC‑TGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCTGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGAt                                                                     <  2:463821/300‑1 (MQ=255)                                                                                                                                                                                                                ggTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCACAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAAtt                                                                 >  2:449006/1‑300 (MQ=255)                                                                                                                                                                                                                 gTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGTTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAATGGGCCAGTGCTGAATGTGGGATTGCCAGTCCCAACCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTc                                                                <  2:629950/300‑1 (MQ=255)                                                                                                                                                                                                                   ttgttgAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCAGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGc                                                              >  1:526894/1‑300 (MQ=255)                                                                                                                                                                                                                        gAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTg                                                         >  2:697626/1‑300 (MQ=255)                                                                                                                                                                                                                                     cgGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATNTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTgcgc                                            <  1:469952/300‑1 (MQ=255)                                                                                                                                                                                                                                      ggCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCAGCAGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGcc                                           >  2:692380/1‑300 (MQ=255)                                                                                                                                                                                                                                                gTCATCGCATTCTCTATCGCTTTATCGATGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGAAAGCTCTGGCGATCTCCCGCCACCCAGGCTCACCGGCAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAGTCCTTC‑GGCCCCCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGCCAGCCACCGCCGTAAAAGGCACAGGCTTTAAAGCCCGAACGGCGTGCGCCCTGTAGGGATAATCCGCCATTGACGAAGCGTGCACCactagtttac                                 >  2:238951/1‑291 (MQ=255)                                                                                                                                                                                                                                                  cATCGCATTCTCTATCGCTTTCTCGTTGAGAATTTCCCGGTTGGTGATTTCTTTTTGACTCAGGTTCAGCGCAGCTCGCTCTGAAGCTCTTTCGATCTCCCGCCACCCATTCCNACCGTAATTGTCCATTTCTGAATGTTTTCTTACCATTCCCGACCCTTCTGGCAACAACC‑GANATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAc                               <  2:553449/300‑1 (MQ=255)                                                                                                                                                                                                                                                  cATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGTTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAc                               <  1:456677/300‑1 (MQ=255)                                                                                                                                                                                                                                                  cATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTTAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTTTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAc                               <  1:428930/300‑1 (MQ=255)                                                                                                                                                                                                                                                  cATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGGGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGTCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTTGGATTGCCAGTCCCAATCCTTC‑TGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAc                               <  2:401904/300‑1 (MQ=255)                                                                                                                                                                                                                                                  cATCGCATTCTCTATCACTCTATCGGTGAGAATTTCCCGTGTTGTGATGTCTTTTCGACTCAGGTTCATCGCAACTCGCGCTGAAGCTCTGTCGATCTCCCTCCACACAGGCTNACCTTAATGGGCCAGGGCTTAATGTGGGATTGCCAGTCCCGATCCTTC‑TGCCGCCAACTTANATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTATAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAc                               <  2:553442/300‑1 (MQ=255)                                                                                                                                                                                                                                                              tATCGCTTTATCGGTGAGAATTTCCCGGTTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTTAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtc                   <  1:694344/300‑1 (MQ=255)                                                                                                                                                                                                                                                              tATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtc                   <  1:117495/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 cGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtcttc                <  1:703902/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tcgttGAGAATTTCCCTGGTGTTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGTGCTGAATGTGGAATTGCCAGTCCCAACCCCTC‑TGCCAACACCCGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATcc         <  2:365634/296‑1 (MQ=255)                                                                                                                                                                                                                                                                        tCGGTGAGAATTTCCCGTGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCCTAAGGTGCCAGTGCTGAATTTGGGATTTCCAGTCCCAATCCTTC‑GGCCAACACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATcc         <  2:357322/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tCGGTGAGAATTTCCCGGGTGGTGATGTTTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGTAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCATGGCTGAATGTGGTATTGCCAGTCCTAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATcc         <  2:372516/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tCGGTGAGAATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTNGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑TGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATNCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATcc         <  2:658989/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               gaATTTCCCGGGTGGTGATGTCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGACGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCgg  >  2:342489/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                         CTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGGGATGTGTAACAGCAGGTTTGTTGAACCACCGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTC‑GGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGG  >  NC_000913/4522954‑4523523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑