| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 339,958 | G→A | D64N (GAC→AAC) | yahH → | putative uncharacterized protein YahH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 339,958 | 0 | G | A | 96.7% | 78.3 / NA | 30 | D64N (GAC→AAC) | yahH | putative uncharacterized protein YahH |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (18/11); total (18/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCG > NC_000913/339672‑340230 | ggCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGCTAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCgg > 1:304345/1‑300 (MQ=255)ggCATTGTGCATCCACCTCTTGCCTGCTTAGAGAAAGCCGTTCTTGGTTGGTGCGAACGTTACGGCGTCTGACCTACATGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCgg > 1:233857/1‑300 (MQ=18) cATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGcc < 1:695333/300‑1 (MQ=255) gCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAGCGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAg < 1:339144/300‑1 (MQ=255) aGCCGTTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGTCGTGAACGCCTTATCCTGCCTACGAGTGGCGCAAGAATCTGTAGNCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGCTGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAg < 2:616473/300‑1 (MQ=17) aGCCATTCTTGGCTGGTGCGAACGTTACGTCGTCTGACCTACATGCTCATGCCAGAGGCGGCGTGAACGCCCTATCCGGCCTACGCATGGCGCAAGAATCTGTAGNCCTAATAAGCGTAGCGCATCATGCACTTGCCACCATTGCCGGGTGCGTCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCCGTAGGCCTGATAAGCTTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCGACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAg < 2:616537/300‑1 (MQ=14) aTTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTa > 1:663224/1‑300 (MQ=255) ttGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGNAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAgcgc < 1:474424/300‑1 (MQ=255) tGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCa > 1:50212/1‑300 (MQ=255) ggTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAgg > 1:665026/1‑300 (MQ=255) ggTGCGAACGTTACGGCGTCTGACCTAAATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGGATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACTATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCCGGCA‑CTTGTCAAAATTGCAGGATGCGGCGTGAACGCCTTATCCGGCCTAAGAGTGGCGCGAGGAT‑TGTAGGCCCGATAAGCGTAGCGCAttcgg > 2:7989/1‑296 (MQ=14) gTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGANTCTGTAGGCCTGATAAGCGTAGCGCCTCAGGCA‑TTTGTCAACATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGGAT‑TGTGGGTCTGATACGTGTAGTGCATCAGGCagtctggc > 2:161520/1‑293 (MQ=11) gTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCagtctggc > 1:259630/1‑293 (MQ=255) gacctacatgttcaggCCGGATGCGGCGTGAACGCCTTATCCGTCCTACGAGTGNCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGNGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAc < 2:647289/285‑1 (MQ=11) tgttcatGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAc < 1:553967/294‑1 (MQ=255) tgaagggcacgttagtattggagtctttATCCGGCCTACGCATAGCGAAGGAGTCTGTATGCCTGATGAGCGCGGCGCATCAGGCACTCTGC‑ACCACTGCCGGATGCGGCCTGGACGCCTTATCCGACCTACGAATGGCGCAAGAACCTGGAGTCCTGATAAGCGTAGCGCATCAGGCATTCTTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAAAATTGTAGGCCTGATAAGCTTAGCGCAGCAGGCAGTCGTTCGTTGGTCATAACCCCACCAccc < 2:183485/274‑1 (MQ=9) gATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCCTCAGGCATTTGTCACCATTGCCGGATTCGTCGTGAACGCCTTCTCCGGCCTAAAAGTGGCGCGAGAATTGTAGGTCTTATAAGCGTATCTCCTCAGGAAATCTGGCGTTGGTCATAAACCCATAACCCTATGTAGCg > 2:256455/1‑300 (MQ=11) gTGAACGCCTTAGCCTGCCGACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGNCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCNTAACCCCATCACCCTCTGTAGCGGACATaac < 2:503612/300‑1 (MQ=14) aaCGCCTTATCCTGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaacaac < 1:372981/300‑1 (MQ=255) ggCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTNGTCAACATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGGAGGCCTGATAAGCGTAGCACATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCATTATCCGGTCTCAGAGTGGCGCGAGTTGTGTCGGCCTGATCCGTGTAGCGCATCATGCAGTCCGGNGTTGGTCATAACACCATCACCATCTGTAGCGGAAATAAAAAAATGAAAGAGcct > 2:643652/1‑298 (MQ=14) gAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATtggt < 1:68834/300‑1 (MQ=255) tCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACCTAACAACATGAAAGAGCTTGTGGTCGTTGCAATTGGTGGc > 1:415434/1‑300 (MQ=255) ggCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTNATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGc > 1:503694/1‑300 (MQ=255) ggCCTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTAACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGc > 1:56447/1‑300 (MQ=255) ccTGATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCAt < 1:575368/300‑1 (MQ=255) gATAAGCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGCCATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTAt > 1:638465/1‑300 (MQ=255) gCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGTGTAGCGCATCAGGCATTTGTCGCCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATCAGCGTAGCGCATCAGGAAGTCTGGCGTTGGTCATAACCCCATCACCCCCTGTCGCGGACATACCAAAATGAAAGAGTTTGTGGTCTTTTCAATTGGTGGCCACAGCATTATCAAAg > 2:383009/1‑300 (MQ=17) gCGTAGCGCATCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAg > 1:14491/1‑300 (MQ=255) tCAGGCA‑TTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATACCAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCCAAGATAACgccag > 1:165411/1‑300 (MQ=255) tttGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCg > 1:361624/1‑300 (MQ=255) | GGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑TTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCG > NC_000913/339672‑340230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |