Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,423,829	G→A	G44S (GGC→AGC)	ulaF →	L‑ribulose‑5‑phosphate 4‑epimerase UlaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,423,829	0	G	A	100.0%	174.2 / NA	53	G44S (GGC→AGC)	ulaF	L‑ribulose‑5‑phosphate 4‑epimerase UlaF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/27);  total (26/27)

GTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAAC  >  NC_000913/4423546‑4424120                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                    gttgttTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAAc                                                                                                                                                                                                                                                                                     <  1:237195/300‑1 (MQ=255)    gttTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCNAAAGCGGGCATGGTGGAGGCGGCATANTGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCAt                                                                                                                                                                                                                                                                                  >  2:476980/1‑300 (MQ=255)               cTCAAACAGAGTGGCTATTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCg                                                                                                                                                                                                                                                                       >  2:391519/1‑300 (MQ=255)               cTCAAACAGAGTGGCTATTGCGGGCAGTACCTGATTGAGATGTGGAGTGAAATGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGGAAGCGCGCATGGCGAAAGCGGGTATGGTGGAAGCGGAATAATGCAAAAGATAAAACAGCAGGTATCTGAAGCCAACACGGAGCTGTCGCGCTACGGGCTGGTGACTTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGACTGCGGGTTGGTGGTGATCAAGAACAGCAGCGTTGCCTACGAAACCATGAAAGCggacg                                                                                                                                                                                                                                                                       >  2:368894/1‑297 (MQ=255)                      agagTGGCTATTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGNGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGNTAtggt                                                                                                                                                                                                                                                                >  2:569462/1‑300 (MQ=255)                       gagTGGCTATTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTNGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGANAtggtg                                                                                                                                                                                                                                                               >  2:572815/1‑300 (MQ=255)                       gagTGGCTATTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATAtggtg                                                                                                                                                                                                                                                               >  1:649414/1‑300 (MQ=255)                        agTGGCTATTGCGGGCCGTACCTGATTGAAATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAAGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCAAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATAtggtgg                                                                                                                                                                                                                                                              >  2:450538/1‑300 (MQ=255)                           ggCTATTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTCCCTGGGGCAACGTCAGCGCTATCGAACGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATGtggtggtgg                                                                                                                                                                                                                                                           >  2:76732/1‑300 (MQ=255)                             cTATTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCNTGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGtt                                                                                                                                                                                                                                                         <  1:491002/300‑1 (MQ=255)                               aTTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGa                                                                                                                                                                                                                                                       >  2:428327/1‑300 (MQ=255)                               aTTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCNGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCATACGAAAACATGAAAGCGGCCGATATGGTGGTGGTTGa                                                                                                                                                                                                                                                       >  2:166769/1‑300 (MQ=255)                                ttGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAGCCATGAAAGCGGCCGATATGGTGGTGGTTGat                                                                                                                                                                                                                                                      <  2:349982/300‑1 (MQ=255)                                     ggCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAg                                                                                                                                                                                                                                                 >  2:542907/1‑300 (MQ=255)                                       ccGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAGCGCGGGCTGGTGGTGATCAAGCCCAGCNGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCg                                                                                                                                                                                                                                               >  2:511894/1‑300 (MQ=255)                                           aCCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGTCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATAGTGGTGGTTGATATGAGCGGCaa                                                                                                                                                                                                                                           <  1:560505/300‑1 (MQ=255)                                              tGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAggt                                                                                                                                                                                                                                        <  1:359450/300‑1 (MQ=255)                                                     gaTGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAANGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGaa                                                                                                                                                                                                                                 <  1:469314/300‑1 (MQ=255)                                                     gaTGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGaa                                                                                                                                                                                                                                 <  1:197840/300‑1 (MQ=255)                                                        gtgGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAggg                                                                                                                                                                                                                              >  1:274926/1‑300 (MQ=255)                                                                      cggAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCGAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATc                                                                                                                                                                                                                <  1:699351/300‑1 (MQ=255)                                                                               cggcggCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGacac                                                                                                                                                                                                       <  1:409737/300‑1 (MQ=255)                                                                                                 aaGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGTAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGTTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAAct                                                                                                                                                                                     <  2:289736/300‑1 (MQ=255)                                                                                                   gcgcGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAAACATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAActct                                                                                                                                                                                   >  2:120921/1‑300 (MQ=255)                                                                                                        tGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACcgt                                                                                                                                                                              <  1:352399/300‑1 (MQ=255)                                                                                                               gTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTAcc                                                                                                                                                                       <  1:74630/300‑1 (MQ=255)                                                                                                                              gCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGATTGGTGGCa                                                                                                                                                        >  2:424674/1‑300 (MQ=255)                                                                                                                                 aaaGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAGACAGCAGGTATTTGAGGCCAACATGGAGCTGCCGCGCTACGTGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCAtgg                                                                                                                                                     <  2:326713/300‑3 (MQ=255)                                                                                                                                         cATGGTGGAGGCGGCATAATGCAGAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTTGTGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGTTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATAcc                                                                                                                                             <  2:208421/300‑1 (MQ=255)                                                                                                                                         cATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATAcc                                                                                                                                             <  1:68711/300‑1 (MQ=255)                                                                                                                                         cATGGTGGAGGCGGCATAATGCAAAAGCGAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATAcc                                                                                                                                             <  1:433054/300‑1 (MQ=255)                                                                                                                                          aTGGTGTAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATAccc                                                                                                                                            <  1:153111/300‑1 (MQ=255)                                                                                                                                           tggtggAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGCCGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACcca                                                                                                                                           >  2:667803/1‑300 (MQ=255)                                                                                                                                             gtggAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTAAGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACccact                                                                                                                                         >  1:107687/1‑300 (MQ=255)                                                                                                                                                 aGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTAGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACccactccac                                                                                                                                     >  2:332394/1‑300 (MQ=255)                                                                                                                                                   gcggcATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCcactccactc                                                                                                                                   <  1:573962/300‑1 (MQ=255)                                                                                                                                                                  aGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATggg                                                                                                                    >  2:424095/1‑300 (MQ=255)                                                                                                                                                                          cagcagGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCg                                                                                                            <  1:541043/300‑1 (MQ=255)                                                                                                                                                                                                  gAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTAGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCAGGCGTTAGGc                                                                                    >  1:326427/1‑300 (MQ=255)                                                                                                                                                                                                  gAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCANGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGc                                                                                    <  1:480590/300‑1 (MQ=255)                                                                                                                                                                                                         cgcgCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACcacgc                                                                             <  1:360134/300‑1 (MQ=255)                                                                                                                                                                                                          gcgcTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACNCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACcacgca                                                                            >  2:622939/1‑300 (MQ=255)                                                                                                                                                                                                                           ccTTTACCTGGGGCAACGTCGGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGACACCACGCACGCCGACTACTTCTTTg                                                           >  2:14643/1‑300 (MQ=255)                                                                                                                                                                                                                                aCCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGAc                                                      >  2:128677/1‑300 (MQ=255)                                                                                                                                                                                                                                    ggggCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTc                                                  <  1:606917/300‑1 (MQ=255)                                                                                                                                                                                                                                      ggCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCg                                                >  2:373082/1‑300 (MQ=255)                                                                                                                                                                                                                                           cGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTAc                                           <  1:193126/300‑1 (MQ=255)                                                                                                                                                                                                                                                  gcTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCggg                                    >  2:266877/1‑300 (MQ=255)                                                                                                                                                                                                                                                  gcTATCGAACGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGTATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCggg                                    >  1:159809/1‑300 (MQ=255)                                                                                                                                                                                                                                                               aaCGCGGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGaagaag                       <  1:6454/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  gcgGGCTGGTGGTGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAgg                    <  1:669101/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         ggtggtGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg             <  1:334388/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         ggtggtGATCAAGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg             >  2:679106/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    aGCCCAGCAGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaac  <  1:40682/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                    GTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAAC  >  NC_000913/4423546‑4424120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑