Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,125,151	C→T	V554I (GTT→ATT)	priA ←	primosomal protein N'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,125,151	0	C	T	100.0%	138.0 / NA	42	V554I (GTT→ATT)	priA	primosomal protein N'
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/14);  total (28/14)

CACAATCACATGGCTGGTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGCCCAGCCCCACGGGGACCAGGTGCGTGGAACCGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCG  >  NC_000913/4124894‑4125441                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                   cacaATCACATGGCTGGTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGAAATCAGCGTCAAATCCGGGAAATGGTGACCTTTCGCCAGCATTtg                                                                                                                                                                                                                                                          >  2:225725/1‑300 (MQ=255)    aaccacaTGGCTGGTCCACGGTGGTAGCTTCATCATTCGCTGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCTGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCGGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTtgggt                                                                                                                                                                                                                                                       <  2:250745/297‑4 (MQ=255)      tcacagtgCTGGTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCTGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCTGGAAATGGTGACCTTTCGCCAGCATTTGTGTAc                                                                                                                                                                                                                                                     <  2:94296/293‑1 (MQ=255)       cacaTGGCTGGTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGNAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTAcc                                                                                                                                                                                                                                                    >  1:508262/1‑300 (MQ=255)       cacaTGGCTGGTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCCGCATTTGTGTAcc                                                                                                                                                                                                                                                    >  2:395617/1‑300 (MQ=255)                 gTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATc                                                                                                                                                                                                                                          >  1:231789/1‑300 (MQ=255)                        gcggTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGCAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCgcgcgc                                                                                                                                                                                                                                   >  1:114712/1‑300 (MQ=255)                                                  gccagcGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTCGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGc                                                                                                                                                                                                         >  1:521823/1‑300 (MQ=255)                                                          cTGTTCGGCAAAGGCGTCGTAGCCTGTATAGAGCAACGTTTGCAACAGGGGATGTTCCGGATGGTGCGTTTGCAGCACCACTACGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCTAAACGCTCTGCCGAGCGAAAATCGTCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCTGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAg                                                                                                                                                                                                 <  2:604079/300‑1 (MQ=255)                                                                         gttgtAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCATTTGCGGCt                                                                                                                                                                                  >  2:635937/4‑300 (MQ=255)                                                                         gtcgtAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCAAATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCt                                                                                                                                                                                  >  2:635648/1‑300 (MQ=255)                                                                              aGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGNTCTGCCGAGCGAAAATCGGCAGAAAANAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCtggtgg                                                                                                                                                                             >  2:462905/1‑300 (MQ=255)                                                                                ccTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTa                                                                                                                                                                           >  1:89799/1‑300 (MQ=255)                                                                                   ttATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATcg                                                                                                                                                                        >  1:408024/1‑300 (MQ=255)                                                                                   ttATAGAGCAACATTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCTGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCTGGAAATTGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGTTGGTATcg                                                                                                                                                                        <  2:242652/300‑1 (MQ=255)                                                                                                        agagGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGTTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGc                                                                                                                                                   <  2:308133/300‑1 (MQ=255)                                                                                                             aTGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCTGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCCGTTGCTGTTCCAGCGCCCCTTTGCGGTTGGTGGTATCGCGGTCGATATGAGAAATGGGCACGcc                                                                                                                                              >  2:229080/1‑300 (MQ=255)                                                                                                               gTTCCGGATGGTGTGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCCATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGTTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCgg                                                                                                                                            >  1:293899/1‑300 (MQ=255)                                                                                                               gTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCgg                                                                                                                                            >  1:14769/1‑300 (MQ=255)                                                                                                                       gggtacGTTTGCAGCACCACTTCTCCCTGTTGACCCGCACGCCCGGCACGACCTGCGACCTGGTTGTAAAGCTGAGCTAAACGCTCTGTCGAGCGAAAATCGGCAGAAAACAGCACGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCTGGAAATGGGGACCTTTCGTCGGCATTTGTGTACCAATTAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGGTCCAGCGCCCCTTTGCGGCTGGTGGTGTCGCGGTCGATACGAGAAATGGGCACGCCGGGGaacaac                                                                                                                                    <  2:25431/295‑1 (MQ=255)                                                                                                                                    gcaCCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCt                                                                                                                       >  1:169343/1‑300 (MQ=255)                                                                                                                                    gcaCCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGACAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGATGGTGGTATCGCGGTCGATACGAGGAATGGGCACGCAGGGGAACAACGGCGCGAGCGTCt                                                                                                                       >  2:115625/1‑300 (MQ=255)                                                                                                                                            tCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGTTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGc                                                                                                               <  2:670001/300‑1 (MQ=255)                                                                                                                                            tCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGc                                                                                                               <  1:457466/300‑1 (MQ=255)                                                                                                                                            tCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTCGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGc                                                                                                               <  2:606468/300‑1 (MQ=255)                                                                                                                                              gCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCAAATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTg                                                                                                             >  1:251669/1‑300 (MQ=255)                                                                                                                                                 cTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCACTCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCAGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTc                                                                                                          >  2:669162/1‑300 (MQ=255)                                                                                                                                                 cTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTc                                                                                                          >  1:162792/1‑300 (MQ=255)                                                                                                                                                   gTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCTCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCgg                                                                                                        >  1:429574/1‑300 (MQ=255)                                                                                                                                                      tACCCGCACGCCCTACACTACCGTCGACCTGGGTGGAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCTGCAGAAAACAGCGTGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGGACCAAGCAAAATCCGCGCGCCGCCGCGATGTACTTCCACCATTTGCCGTGCCAGCACCCCTTTGCGGCTGGTGGTATCGCAGTCGATACGAGAAATGGGTACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGc                                                                                                     <  2:268391/300‑1 (MQ=255)                                                                                                                                                      tACCCGCACGCCCGGCACGACCTGCGACCTGGGTGTAAAGCTGAGCGAAACGCTNTGCCGAGCGAAAATCGGCAGAAAACAGCGCGTCGTCCACGTCCATGAATGCAATCAGCTTCACATCCGTGAAATGGTGACCTTTCGCCAGCATTTGTGGACCAATCAAAATCCGCGCACCACCGCGATGTACTTCTGCCATGTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACNAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGc                                                                                                     <  2:650385/300‑1 (MQ=255)                                                                                                                                                                          ccGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGCCCAGCCCCACGGGGACCAgg                                                                                 >  1:174703/1‑300 (MQ=255)                                                                                                                                                                           cGGCGANCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGNAAACAGCGCGCCGTCCACGGCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGCCCAGCCCCACGGGGACCAGGt                                                                                <  2:580907/300‑1 (MQ=255)                                                                                                                                                                                      gtgtAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGCCCAGCCCCACGGGGACCAGGTGCGTGGAACCg                                                                     >  2:451904/1‑300 (MQ=255)                                                                                                                                                                                      gtgtAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGCCCAGCCCCACGGGGACCAGGTGCGTGGAACCg                                                                     >  1:367209/1‑300 (MQ=255)                                                                                                                                                                                                  gtgaagcGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTAGCCAGTATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCAGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGTGCGAGCGTATGTTCAAGCTGTTCGGTGCCCAGCACCAAGGGGACCAGGTGCGTGGACCCGCAGGagtggcat                                                         >  2:173387/7‑293 (MQ=255)                                                                                                                                                                                                         gCTCTGCCGAGCGAAACTCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGCCCAGCCCCACGGGGACCAGGTGCGTGGAACCGCAGGAAGGGCACTGGcgcg                                                  >  1:709140/1‑300 (MQ=255)                                                                                                                                                                                                         gCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGNGGCTGGTGGTATCGCGGTCGCTACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCACGCTGTTCGGTGCCCAGCCTCACGGGGACCAGGTGTGTGGAGCCGCAGGAAGGACACTGGcgcg                                                  >  2:170457/1‑300 (MQ=255)                                                                                                                                                                                                                  aGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGCCCAGCCCCACGGGGACCAGGTGCGTGGAACCGCAGGAAGGGCACTGGCGCGGCACCGGAc                                         <  1:199543/300‑1 (MQ=255)                                                                                                                                                                                                                                       gcgcgcCGTCCACGTCCAGTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGCCCAGCCCCACGGGGACCAGGTGCGTGGAACCGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGc                    <  1:451578/300‑1 (MQ=255)                                                                                                                                                                                                                                              gtccacgtccaGTACTGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGCCCAGCCCCACGGGGACCAGGTGCGTGGAACCGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAg             >  1:448596/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gTAATGCAATCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTANCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGCCCAGCCCCACGGGGACCAGGTGCGTGGAACCGCAGGAAGGGCACNGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTgcg  >  2:648859/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                   CACAATCACATGGCTGGTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGCCAGTTGCTGTTCCAGCGCCCCTTTGCGGCTGGTGGTATCGCGGTCGATACGAGAAATGGGCACGCCGGGGAACAACGGCGCGAGCGTCTGTTCAAGCTGTTCGGTGCCCAGCCCCACGGGGACCAGGTGCGTGGAACCGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCG  >  NC_000913/4124894‑4125441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑