Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,983,395	C→T	G146G (GGC→GGT)	aslB →	putative anaerobic sulfatase maturation enzyme AslB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,983,395	0	C	T	100.0%	325.0 / NA	93	G146G (GGC→GGT)	aslB	putative anaerobic sulfatase maturation enzyme AslB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/45);  total (48/45)

GACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTCGGTGAGTTTCTGGTG  >  NC_000913/3983099‑3983683                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                 gACACGCTGTAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAATCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGa                                                                                                                                                                                                                                                                                               <  2:147187/300‑1 (MQ=255)   cacGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGAcc                                                                                                                                                                                                                                                                                             <  1:81544/300‑1 (MQ=255)       cTGGACGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTAAAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCCTCCAGACTAACGGCGTGCTGCTGGATGACGAATAGTGCGCGTTTCTCGCGGAGCATCATTTTTTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGCTCCACAATCAATATCGCGTGACTAAAGGCGGTAGACCCACg                                                                                                                                                                                                                                                                                         >  2:234695/1‑300 (MQ=255)       cTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTACAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTATTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGATAGACCCACg                                                                                                                                                                                                                                                                                         >  2:234335/1‑300 (MQ=255)         ggAAGCGTATGTCCGTCACTATATCGCTGCCNGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGNCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCa                                                                                                                                                                                                                                                                                       >  2:502433/1‑300 (MQ=255)         ggAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCa                                                                                                                                                                                                                                                                                       <  1:597398/300‑1 (MQ=255)             gCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAg                                                                                                                                                                                                                                                                                   >  1:50387/1‑300 (MQ=255)              cGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGc                                                                                                                                                                                                                                                                                  >  1:365287/1‑300 (MQ=255)              cGTATGGCCGTCACTATATCGCGGCCAGCGAACCGCAAAACGAGGTTGCTTTTATCTGGCAGGGCGGCGAACCAACGCTGCTCGGGCTGGCGTTTTACCACCGTGCTGTAGCGCTGCAGGCGAAACATGGTGTTGGCAGGAAGATAAGTAACAGCGTCTAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTGCTCTCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGc                                                                                                                                                                                                                                                                                  <  2:235570/300‑1 (MQ=255)                   gTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGANATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAANCTGGTg                                                                                                                                                                                                                                                                             >  2:575886/1‑300 (MQ=255)                    tCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGTCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGa                                                                                                                                                                                                                                                                            <  2:394436/300‑1 (MQ=255)                    tCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGa                                                                                                                                                                                                                                                                            >  2:448352/1‑300 (MQ=255)                    tCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGa                                                                                                                                                                                                                                                                            <  1:667435/300‑1 (MQ=255)                    tCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCGTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGa                                                                                                                                                                                                                                                                            <  1:689448/300‑1 (MQ=255)                      cGTCACTATATCGCTGCCAGCGAACCGCAAAACGAATTGGCTTCTACCTGGCAGGGCGGCGAACCAACGCTACTCGTACTGGCTTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTTGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAACATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATg                                                                                                                                                                                                                                                                          <  2:46049/300‑1 (MQ=255)                        tCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCg                                                                                                                                                                                                                                                                        >  2:677275/1‑300 (MQ=255)                           cTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGACGATAAGTAACAGCTTCCAGACTAAAGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTAGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGtac                                                                                                                                                                                                                                                                     >  1:157740/1‑298 (MQ=255)                           cTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCAGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGc                                                                                                                                                                                                                                                                     >  2:290038/1‑300 (MQ=255)                                   cTGCCAGCGAACCGCAAAACTAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAGCGCTACTCGTGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACTGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCTTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGc                                                                                                                                                                                                                                                             <  2:135672/300‑1 (MQ=255)                                                gCAAAACGAAGTGGCTTTGACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGTCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAAca                                                                                                                                                                                                                                                <  2:684642/300‑1 (MQ=255)                                                             gCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCACGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGAATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACtat                                                                                                                                                                                                                                   >  2:240212/1‑300 (MQ=255)                                                                 ttACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACg                                                                                                                                                                                                                               <  1:704509/300‑1 (MQ=255)                                                                  tACCTTGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCNGTAGCGCTACAGTCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCANAAGCTGTTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGt                                                                                                                                                                                                                              <  2:643644/300‑1 (MQ=255)                                                                                   aaCCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGTATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATc                                                                                                                                                                                                             >  2:77313/1‑300 (MQ=255)                                                                                   aaCCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATc                                                                                                                                                                                                             >  2:378274/1‑300 (MQ=255)                                                                                   aaCCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATc                                                                                                                                                                                                             <  1:270539/300‑1 (MQ=255)                                                                                   aaCCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGAAGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATc                                                                                                                                                                                                             >  2:430715/1‑300 (MQ=255)                                                                                        aCGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCAcc                                                                                                                                                                                                        >  2:134937/1‑300 (MQ=255)                                                                                        aCGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCAcc                                                                                                                                                                                                        <  1:658256/300‑1 (MQ=255)                                                                                        aCGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTNTCTCGCGGAGCATCATTTTCTTGTTGNTTTATCGCTGGATGGCCCGCCTGAGATCCACAATGAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCAca                                                                                                                                                                                                        >  2:456335/1‑299 (MQ=255)                                                                                             aCTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAgcgc                                                                                                                                                                                                   <  1:181332/300‑1 (MQ=255)                                                                                                       gCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCg                                                                                                                                                                                         >  2:667556/1‑300 (MQ=255)                                                                                                             tACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAg                                                                                                                                                                                   <  1:311089/300‑1 (MQ=255)                                                                                                              aCCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGTTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAgg                                                                                                                                                                                  <  1:311683/300‑1 (MQ=255)                                                                                                              aCCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAgg                                                                                                                                                                                  <  1:89813/300‑1 (MQ=255)                                                                                                                      gccgtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGAt                                                                                                                                                                          >  2:13745/1‑300 (MQ=255)                                                                                                                                        aaaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCNTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGNGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGa                                                                                                                                                        >  2:548184/1‑300 (MQ=255)                                                                                                                                        aaaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGNCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGa                                                                                                                                                        >  1:500082/1‑300 (MQ=255)                                                                                                                                            gtGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAGCCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCg                                                                                                                                                    >  2:605935/2‑300 (MQ=255)                                                                                                                                                 gCTGGCAGGAAGATAAGTAACAACTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTc                                                                                                                                               <  1:42326/300‑1 (MQ=255)                                                                                                                                                    ggCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTACTCGCACCAGCGCNCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATc                                                                                                                                            >  2:525266/1‑300 (MQ=255)                                                                                                                                                               aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTcc                                                                                                                                 <  1:627780/300‑1 (MQ=255)                                                                                                                                                                aGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCg                                                                                                                                >  2:270580/1‑300 (MQ=255)                                                                                                                                                                 gTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCgg                                                                                                                               <  1:284227/300‑1 (MQ=255)                                                                                                                                                                       gCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGGTCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGCCTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACAGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCAGTGGTCg                                                                                                                         >  2:39322/1‑300 (MQ=255)                                                                                                                                                                       gCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCg                                                                                                                         >  1:415705/1‑300 (MQ=255)                                                                                                                                                                            cAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAgcgc                                                                                                                    <  1:548255/300‑1 (MQ=255)                                                                                                                                                                             aGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGcc                                                                                                                   <  1:448128/300‑1 (MQ=255)                                                                                                                                                                              gACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCTACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCt                                                                                                                  >  1:362406/1‑300 (MQ=255)                                                                                                                                                                              gACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCt                                                                                                                  >  2:412213/1‑300 (MQ=255)                                                                                                                                                                                cTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCACTGACGCTCCTGCAAAAACCCCATGTCGACTATAACGTGCTGGTATGCGTTAATCGCCCCAGTGCGCAGCAACTGTTGCAGGTATATAATTTTTTGTGTGCTGCGGGCGTCGACTTCGTCCAGTTTCTTCCTGTGGTCgcgctcatgg                                                                                                                >  2:190239/1‑291 (MQ=255)                                                                                                                                                                                cTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTAGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTgg                                                                                                                >  2:189640/1‑300 (MQ=255)                                                                                                                                                                                 tAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGc                                                                                                               <  1:57563/300‑1 (MQ=255)                                                                                                                                                                                     ggCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCANGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGNTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCtgct                                                                                                           >  2:564363/1‑298 (MQ=255)                                                                                                                                                                                       cGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCtgatga                                                                                                         <  1:167195/300‑1 (MQ=255)                                                                                                                                                                                        gTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGaa                                                                                                        >  1:426851/1‑300 (MQ=255)                                                                                                                                                                                             gctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAaacaac                                                                                                   >  1:53764/1‑300 (MQ=255)                                                                                                                                                                                             gctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGATGGATGGCCCGCATGAGATCCACAATCGATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGTGTGACCTGAAGCTCCTGCAAAAACACCATGTCGACTATACCGTGTTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATTATTTTTTGTGCGATGCGGGAGTAGAATTCGTCCAGTTTATTCCGTTGGTCAAGCGCCTGGCTGATGAaacaac                                                                                                   >  1:53640/1‑300 (MQ=255)                                                                                                                                                                                               tgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCTACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTg                                                                                                 <  2:359027/300‑1 (MQ=255)                                                                                                                                                                                                ggATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGc                                                                                                >  2:101219/1‑300 (MQ=255)                                                                                                                                                                                                    gACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATCTGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCcgc                                                                                            >  2:418699/1‑300 (MQ=255)                                                                                                                                                                                                                      ctcGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACAt                                                                          <  1:170329/300‑1 (MQ=255)                                                                                                                                                                                                                       tcGCGGAACATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAGTCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATg                                                                         <  2:115367/300‑1 (MQ=255)                                                                                                                                                                                                                            gAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGCCCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAAACGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTATATGCGCCt                                                                    >  2:67188/1‑300 (MQ=255)                                                                                                                                                                                                                            gAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATANGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCt                                                                    >  2:609149/1‑300 (MQ=255)                                                                                                                                                                                                                              acatccctttCTTTTTGTTTTATCGCTGGATGACCCGCCTTAGATCCACAATCACTATCGCGTGACTAAAGGTGGTAGACCCACTCATAAGCTGGTGATGCGTGCCCTGACGCTCCGGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTgg                                                                  <  2:437246/293‑1 (MQ=255)                                                                                                                                                                                                                                 tcatTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGNTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGa                                                               <  1:474501/300‑1 (MQ=255)                                                                                                                                                                                                                                 tcatTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGTGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCATGGTGa                                                               >  2:239781/1‑300 (MQ=255)                                                                                                                                                                                                                                      ttCTTTTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAAGATCGCGGGACTAAAGGTGGTAGACCCACACATAAGCTGTTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTc                                                          <  2:146686/300‑1 (MQ=255)                                                                                                                                                                                                                                      ttCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGTTGATGCGTGCCCTGACGCTCCTNCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTc                                                          <  1:505876/300‑1 (MQ=255)                                                                                                                                                                                                                                      ttCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTc                                                          <  2:389184/300‑1 (MQ=255)                                                                                                                                                                                                                                      ttCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTc                                                          <  1:651977/300‑1 (MQ=255)                                                                                                                                                                                                                                      ttCTTGTTGGGTTGTCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGNGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAGCGTGCTGGTCTGCGTTAATCGCACCAGCGCACAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATGTTc                                                          <  2:519734/300‑1 (MQ=255)                                                                                                                                                                                                                                                     cGCTGGATTGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACgg                                           <  1:103166/300‑1 (MQ=255)                                                                                                                                                                                                                                                       cTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGaa                                         <  1:308337/300‑1 (MQ=255)                                                                                                                                                                                                                                                           agtgCCCGCCTGAGATCCACAAGCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGGCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCANCCGTTGCAGGTATATGATTTTTTGTGNGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGTTGATATTCAGGGTGAGCTAACGGAATGGt                                     <  2:477631/297‑1 (MQ=255)                                                                                                                                                                                                                                                            tGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTc                                    >  1:365082/1‑300 (MQ=255)                                                                                                                                                                                                                                                                ccGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGGCTGCGTTAATCGCACCAGCGCGCAACAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTg                                <  1:622496/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 cGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCACACCGGCGCGCAGCAACCGTTGCAAGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTgc                               <  2:323868/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      gagaTCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCANCAGCGCGCAGCAACCGTTGCAGGTATNTGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGccca                          >  2:476999/1‑299 (MQ=255)                                                                                                                                                                                                                                                                         aTCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCgag                       >  1:685653/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          tCCACANTCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGANGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCgagg                      <  2:585263/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tCCACAATCAATATCGCGTGACTAAAGTTGGTAGACCCACGCATAAGCTGTTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCgagg                      <  1:120732/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGTTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCgagg                      <  1:400117/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tCCACAATCAATATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCgagg                      <  1:288696/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tCCACAATCAATATCGCGTGACCAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCgagg                      <  1:630739/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              caatcaatATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGtt                  >  2:384953/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              caatcaatATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGtt                  >  1:151322/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               aatcaatATCGCGTGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCNCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCNCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTc                 >  2:658351/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            tGACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTCGGTGAGTTTCtgg    >  1:123382/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             gACTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTCGGTGAGTTTCtggt   >  1:27380/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              aCTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTCGGTGAGTTTCtggtg  >  1:594621/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              aCTAAAGGTGGTAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACGTAAGTTACATGCGCCTGGTGATATTCANGGTGAGCTAACGGAATGGTCGGTGCGCACCGAGGAGTTCGGTGAGTTTCtggtg  >  2:527338/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                 GACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGACTATAACGTGCTGGTCTGCGTTAATCGCACCAGCGCGCAGCAACCGTTGCAGGTATATGATTTTTTGTGCGATGCGGGAGTCGAATTCATCCAGTTTATTCCGGTGGTCGAGCGCCTGGCTGATGAAACAACTGCCCGCGATGGACTTAAGTTACATGCGCCTGGTGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTCGGTGAGTTTCTGGTG  >  NC_000913/3983099‑3983683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑