Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,622,465 C→T P1092S (CCG→TCG)  rhsB → rhs element protein RhsB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,622,4650CT98.0% 159.0 / NA 50P1092S (CCG→TCG) rhsBrhs element protein RhsB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (25/24);  minor base G (0/1);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTG‑GGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCC  >  NC_000913/3622202‑3622759
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      
gggTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTG‑GGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGaaa                                                                                                                                                                                                                                                                    >  1:385030/1‑300 (MQ=255)
                         gccaggCTGGATGTCGCTGTCATGGAAGCCACGAGTGACCTGGTA‑GTGTGTGAACTTCTACCGGCGGACCACGATACGGAACGACGGGAGCCGCATCCAGACGACTTATCAGCCGGTGAGCTTTACGCTACTCACCAGGGTCGAAACTACCACCGGGGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGAAGTTGGCAGTGTGGTGGTCGCACCGGTGCTGGTGCAGATGCTAGACCGGCTGGAAAGTGAAATCCTGGCTGGCCGGgggag                                                                                                                                                                                                                                           <  2:99132/296‑1 (MQ=9)
                                           gTCACGGAAACCGCAAGTGACCTGGTACGGCTG‑GGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctg                                                                                                                                                                                                                         >  1:219539/1‑300 (MQ=39)
                                                        caattGACCTGGTACGGCTG‑GGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCTGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTNCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGANTGAGGAAAGCCGCCGCTGGCTGGCATCGTGc                                                                                                                                                                                                            <  2:482433/296‑1 (MQ=255)
                                                              aCCTGGTACGGCTG‑GGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGAACGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTg                                                                                                                                                                                                      >  2:303342/1‑300 (MQ=255)
                                                                 tGGTACGGCTG‑GGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGAcc                                                                                                                                                                                                   >  1:61555/1‑300 (MQ=39)
                                                                  ggTACGGCTG‑GGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCg                                                                                                                                                                                                  >  2:22000/1‑300 (MQ=255)
                                                                  ggTACGGCTG‑GGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCg                                                                                                                                                                                                  >  1:515341/1‑300 (MQ=255)
                                                                   gTACGGCTG‑GGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGACGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGAACGt                                                                                                                                                                                                 >  2:43506/1‑300 (MQ=255)
                                                                        gCTG‑GGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACTGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGc                                                                                                                                                                                            <  2:409036/300‑1 (MQ=255)
                                                                           g‑ggACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGAACGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGa                                                                                                                                                                                         >  2:407926/1‑300 (MQ=255)
                                                                                  gCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCAATCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGACGGTGCTGGTGCAGATGCTCGACCGGCTGGACAGTGAAATCCTGGCTGACCGGGTGAGTGAGGCAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCCGATGCaaa                                                                                                                                                                                   >  2:253555/1‑300 (MQ=255)
                                                                                         gCTGACCACGATACCGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGACGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCATGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCACGGAGCAGATGCAAAACCAGac                                                                                                                                                                            >  2:678051/1‑299 (MQ=255)
                                                                                         gCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGAt                                                                                                                                                                            <  1:323363/300‑1 (MQ=39)
                                                                                               cACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGAccc                                                                                                                                                                      <  1:153823/300‑1 (MQ=39)
                                                                                                        gAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTacac                                                                                                                                                             <  1:339779/300‑1 (MQ=39)
                                                                                                         aaCGGCAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGTCGGATGCCCTTCAGCAGTCCGTCNGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGATGCAGATGCTCGACCGGCTGGAAAGTGAGATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACg                                                                                                                                                            <  2:170709/300‑1 (MQ=255)
                                                                                                                       cATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGAACGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCAGCTGGCTGGCATCGTGCGGCCTAACCGTGGAGCAGATGCAAAACAAGATGGACCCGGTGTACACGCCGGCGCGAAAAAt                                                                                                                                              >  2:92805/1‑300 (MQ=255)
                                                                                                                             gACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGAACGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGGGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCt                                                                                                                                        >  1:83460/1‑300 (MQ=25)
                                                                                                                                 aTTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGAACGGCTGGAAAGTGAAATCCTAGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCATGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTAc                                                                                                                                    >  2:367154/1‑300 (MQ=255)
                                                                                                                                  tttATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTAcc                                                                                                                                   <  1:272772/300‑1 (MQ=25)
                                                                                                                                    tATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGNAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCAc                                                                                                                                 <  1:493772/300‑1 (MQ=25)
                                                                                                                                      tCAGTCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCACAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGTCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCTGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTg                                                                                                                               <  1:21126/300‑1 (MQ=255)
                                                                                                                                      tCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTg                                                                                                                               <  1:171407/300‑1 (MQ=25)
                                                                                                                                      tCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCAGGCGCGAAAAATCCACCTGTACCACTg                                                                                                                               <  1:421174/300‑1 (MQ=25)
                                                                                                                                            ggggAGCTTCACGCCACTCATCAGGGTCGAAACTGCCAACGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGACGGTGCTGGTGCAAATGCTCGAACGGCTGGAAAGTGAAGTCCTGGCTGACCGGGTTCTTGAGGAAAGCAGCCGCTGGCTGGCATCGTGCGTCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCAGGTGTACACGCCGGCGCGAAAAATCCACCTGCACCACTGCGACCa                                                                                                                         >  1:192152/1‑300 (MQ=11)
                                                                                                                                                 gCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATcgcg                                                                                                                    >  2:43151/1‑300 (MQ=25)
                                                                                                                                                              catcaGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCATGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGgc                                                                                                       >  2:444993/1‑300 (MQ=25)
                                                                                                                                                                    ggTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACGTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCtcat                                                                                                 >  2:604173/1‑300 (MQ=37)
                                                                                                                                                                                    ggTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTTGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCa                                                                                 <  1:273100/300‑1 (MQ=25)
                                                                                                                                                                                    ggTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCa                                                                                 <  1:221314/300‑1 (MQ=25)
                                                                                                                                                                                        aGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAg                                                                             <  1:419508/300‑1 (MQ=25)
                                                                                                                                                                                         gCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGc                                                                            <  1:227839/300‑1 (MQ=25)
                                                                                                                                                                                                   aCGCAGCGCCGCAGCCTGGCGGATGCCCTTCNGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCNGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCa                                                                  >  2:504768/1‑300 (MQ=255)
                                                                                                                                                                                                     gCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGa                                                                <  1:653311/300‑1 (MQ=39)
                                                                                                                                                                                                       aGCGCCGCAGACGGGCGGAGGCCCTTCAGCAGTCCGGCGGCGAAAACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGTGGCCTGACCGTGGAGCAGATGCAAAACCAGATGTACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAat                                                              <  2:58324/300‑1 (MQ=255)
                                                                                                                                                                                                         cgccgcAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAatat                                                            <  1:664951/300‑1 (MQ=39)
                                                                                                                                                                                                           ccgcAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATTAGCACGGAAGGGGCAACAACGTGGTGCGCAGAACatga                                                          >  2:3487/1‑300 (MQ=255)
                                                                                                                                                                                                            cgcAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATatgat                                                         <  1:427359/300‑1 (MQ=255)
                                                                                                                                                                                                               agcatGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATAtgatgag                                                      >  2:29085/5‑299 (MQ=255)
                                                                                                                                                                                                                            cccTTCAGCAGTCCGGCGGCGAAGACGGTGGNAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGNCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAAAAGCGTGGTGCGCAGAATATGATGAATGGGGCAACctgc                                         >  2:499268/1‑300 (MQ=255)
                                                                                                                                                                                                                            cccTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACctgc                                         >  1:195200/1‑300 (MQ=39)
                                                                                                                                                                                                                            cccTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCTCGACGGTGCTGGTGCAGATGCTCGAACGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGNTGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGAGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACctgc                                         >  2:162743/1‑300 (MQ=255)
                                                                                                                                                                                                                                                 aaGACGGTGGCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATc                    <  1:93651/300‑1 (MQ=39)
                                                                                                                                                                                                                                                  agacgtttgCAGTGTGGTGTTCTCGCCGTTGCTGGTGCAGATGCTCGACCGGCTNGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGTACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGATCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGTAGGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCa                   <  2:521358/292‑1 (MQ=14)
                                                                                                                                                                                                                                                  aGACGGTGGCAGTTTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCa                   <  1:454660/300‑1 (MQ=255)
                                                                                                                                                                                                                                                         ggCAGTGTGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgcag            <  1:42465/300‑1 (MQ=39)
                                                                                                                                                                                                                                                             gtgtGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGNGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCagcagc        >  1:493903/1‑300 (MQ=39)
                                                                                                                                                                                                                                                             gtgtGGTGTTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCagcagc        >  2:404627/1‑300 (MQ=39)
                                                                                                                                                                                                                                                                   tgtTCTCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATcc  <  1:696646/300‑1 (MQ=39)
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      
GGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTG‑GGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCC  >  NC_000913/3622202‑3622759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑