Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,324	G→A	G179D (GGC→GAC)	yagH →	putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,324	0	G	A	95.3%	136.6 / NA	43	G179D (GGC→GAC)	yagH	putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (15/26);  minor base C (2/0);  total (17/26)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTG  >  NC_000913/287037‑287616                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                     aTTCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGCCACGCCGCTCTg                                                                                                                                                                                                                                                                                          >  2:683008/1‑300 (MQ=255)  gccgggCTGCTCTACACCGACGGGAAGATTGTCGACTCGCCTTGGAAAAACGGCCACAACTTCCTCGTCACCGCACCCTCCATCGAGGGGCCATTGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCACCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGc                                                                                                                                                                                                                                                                                         <  2:90980/296‑1 (MQ=255)     tGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTac                                                                                                                                                                                                                                                                                      <  1:565870/300‑1 (MQ=255)       gctgctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACNATGGCCGCAAATACTATATCTACCGCNCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACATGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTacac                                                                                                                                                                                                                                                                                    <  2:473578/300‑1 (MQ=255)       gctgctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTacac                                                                                                                                                                                                                                                                                    <  1:200301/300‑1 (MQ=255)       gctgctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCACGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCACCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTacac                                                                                                                                                                                                                                                                                    <  2:692039/300‑1 (MQ=255)       caggctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCACAACTTCCTCGTCACCGCACCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTGGACCCGTCCCGGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCTGGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTacac                                                                                                                                                                                                                                                                                    <  2:539714/297‑1 (MQ=255)          gctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACAcctg                                                                                                                                                                                                                                                                                 >  1:548967/1‑298 (MQ=255)          gctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGACCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGa                                                                                                                                                                                                                                                                                 >  1:217978/1‑300 (MQ=255)          gctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTCCCNCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGAACCGTCCCTGTTCCACGCCGACGATGGCCGCAAATACCATATCTACCGCACGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTAAAGGCGTTTGCCCCGCAGACCGGCANGCCCTCGACCGAGTGTAAAACGCTGTTTACCGACACGCCGCTCTGCTaaacaga                                                                                                                                                                                                                                                                                 >  2:648469/1‑294 (MQ=255)             ctACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCAATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGNCCCTGTTCCACGACGACGATGGCCGCCAATACTATATCTACCGCCCGTGAGGGCCGCGCCANCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCAGCACGCTCTCGCCCGGGCTCACAACGCGGTTTACCGCCACGCCGCTCTGCTACACCGAAga                                                                                                                                                                                                                                                                              >  2:564725/1‑299 (MQ=255)                          aaGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCTTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTAt                                                                                                                                                                                                                                                                 <  1:7548/300‑1 (MQ=255)                          aaGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTAt                                                                                                                                                                                                                                                                 <  1:4566/300‑1 (MQ=255)                                         ccGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATgg                                                                                                                                                                                                                                                  >  1:240965/1‑300 (MQ=255)                                            tGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATATTATATCGACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTGACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGtac                                                                                                                                                                                                                                               <  1:209540/300‑1 (MQ=255)                                            tGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCTGGATGGtac                                                                                                                                                                                                                                               <  1:649416/300‑1 (MQ=255)                                                 aaaCTGCCGCAACTTCCTCGTCACCGCGCCTTCCATCTAGGGGCCATGGAGCGAGCCAATCCCGATTGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCt                                                                                                                                                                                                                                          <  1:317336/300‑1 (MQ=255)                                                 aaaCGGCTGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCACCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCt                                                                                                                                                                                                                                          <  2:237052/300‑1 (MQ=255)                                                 aaaCGGCCGCAACTTCCTCGTCACCGCGCCCTCCGTCTAGGGGCCATGGAGCGAGCCAATCTCGATGGGCAACGGTGGGTTTGACTCGTCCCTGTTCCACGACGACGATTGCCGCAAATACTATATCTACCTCCCTTGGGGGCCGCGCCACCACAACAACCCACACAACACCATTGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAACCGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCt                                                                                                                                                                                                                                          <  2:344809/300‑1 (MQ=255)                                                                             cccTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCNCAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGNCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCAAGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTAc                                                                                                                                                                                                              >  2:561416/1‑300 (MQ=255)                                                                              ccTCCATCGAGTGGCCGTGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGTCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACg                                                                                                                                                                                                             <  2:318104/300‑1 (MQ=255)                                                                               cTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACTGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGTTACGa                                                                                                                                                                                                            >  2:137945/1‑300 (MQ=255)                                                                                             cATGGAGCGAGCCAATCCCGATGGGCAACGGCGGTTTTGACCCTTCCCTGTTCCACGACGACGGTGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACTGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCgtgg                                                                                                                                                                                              <  1:61571/300‑1 (MQ=255)                                                                                                ggAGCGAGCAAATCCCGATGGGCAACGGCGGGTTGGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCCCCCGTGGGGGCCGCGCCACCACAGCAACNCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGNAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGtgc                                                                                                                                                                                           <  2:547445/300‑1 (MQ=255)                                                                                                                          ggcggGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCg                                                                                                                                                                 >  1:106496/1‑300 (MQ=255)                                                                                                                              ggTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGTGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACg                                                                                                                                                             >  1:15510/1‑300 (MQ=255)                                                                                                                              ggTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACg                                                                                                                                                             >  1:299184/1‑300 (MQ=255)                                                                                                                                                           gaTGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTTTTACAGGCGTTTGACCCGCAGACCGGCACACTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGAcc                                                                                                                                <  1:349157/300‑1 (MQ=255)                                                                                                                                                                     gatactgggtttgccGCCCTTGGGGGCCGCACCACCACAGCAACCCGCCCAACACCACCGGGGTACAGGTGGTTGACCCGCAGACCGGCACGCGCTCGCCCGAGCGCAAAATTCTGTTGACCGACACGCCGCTCTGCTACACCGAAGANACGCACCTGTATCGCCACGCTGGATGNTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGCCGTGCCGTACGAGCGGCGCCCGGACGTAACGATGATGACCAGCTGGCAcc                                                                                                                      <  2:457953/287‑1 (MQ=255)                                                                                                                                                                                  ccGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAAccc                                                                                                         <  1:378971/300‑1 (MQ=255)                                                                                                                                                                                            gtgCCGCGCCACCACAGCAACCCGCACAACACCCTCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGNCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGCATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCagaag                                                                                               <  2:609691/298‑1 (MQ=255)                                                                                                                                                                                              gctgcgcCACCAAAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGAACGCTCTCGCCCGAGCGCAAAACGTTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCTGGAGGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAagag                                                                                             <  2:278080/297‑1 (MQ=255)                                                                                                                                                                                                             cAACCCTCCCAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCgct                                                                              <  2:390608/300‑1 (MQ=255)                                                                                                                                                                                                                   gCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCa                                                                        >  1:566547/1‑300 (MQ=255)                                                                                                                                                                                                                        ccacCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTAAACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCAGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGc                                                                   >  1:635071/2‑300 (MQ=255)                                                                                                                                                                                                                         cacCATCGTGTTACAGGCGTTTGACCCGCAGACTGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACTGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCa                                                                  <  2:229845/300‑1 (MQ=255)                                                                                                                                                                                                                                   ttACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGaa                                                        >  1:519499/1‑300 (MQ=255)                                                                                                                                                                                                                                          cGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACa                                                 >  1:373376/1‑300 (MQ=255)                                                                                                                                                                                                                                              tGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACNCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACAAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGc                                             <  2:509899/300‑1 (MQ=255)                                                                                                                                                                                                                                              tGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGc                                             <  1:69360/300‑1 (MQ=255)                                                                                                                                                                                                                                                           ggCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGNAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAgc                                >  1:504652/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGccgccc                           >  1:48091/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         tttACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCTTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCgctg  <  2:281966/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                     ATTCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTG  >  NC_000913/287037‑287616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑