Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,222,872	G→A	W80* (TGG→TGA)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,222,872	0	G	A	100.0%	306.9 / NA	89	W80* (TGG→TGA)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/52);  total (37/52)

ACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTG  >  NC_000913/3222579‑3223155                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                            acacAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCCGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATg                                                                                                                                                                                                                                                                                       >  2:254628/1‑300 (MQ=255)  cacaAACTACCATCACCATGAATGGTTCCGATTTCTCTCGACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATgg                                                                                                                                                                                                                                                                                      <  2:121053/300‑1 (MQ=255)      aaCTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAgg                                                                                                                                                                                                                                                                                  >  2:544865/1‑300 (MQ=255)       acgaCCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTGTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCAGGTGACAAATGGAAGGt                                                                                                                                                                                                                                                                                 <  1:619513/297‑1 (MQ=255)       aCTACCATCACCATGAATGGTTCCGATTTCTCTCTACCTGGAGGCCCTATGAATCGCTGGTAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGt                                                                                                                                                                                                                                                                                 <  1:619503/300‑1 (MQ=255)       aCTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGt                                                                                                                                                                                                                                                                                 <  1:320794/300‑1 (MQ=255)        cTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTc                                                                                                                                                                                                                                                                                >  1:660327/1‑300 (MQ=255)                aCCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCaaa                                                                                                                                                                                                                                                                        >  1:166286/1‑300 (MQ=255)                 ccATGAATGGTTCCGCTTTCTCTATACCGGGAGGCCCTATGGATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCAACTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAACTGGAAGGTCACGGCAAAc                                                                                                                                                                                                                                                                       >  2:591501/1‑300 (MQ=255)                 ccATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTACGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTATGAGTTAATGGCTGACTGGGGGCATATTACCGTCCACGCCATGTGACAAATGGAAGGTCACGGCAAAc                                                                                                                                                                                                                                                                       >  2:5152/1‑300 (MQ=255)                 ccATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAAc                                                                                                                                                                                                                                                                       >  1:544785/1‑300 (MQ=255)                   aTGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCGGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTg                                                                                                                                                                                                                                                                     <  2:340469/300‑1 (MQ=255)                     gAATGGTTCCGATTTCTCTCTAACGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCa                                                                                                                                                                                                                                                                   >  2:91213/1‑300 (MQ=255)                        tGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAata                                                                                                                                                                                                                                                                >  1:591825/1‑300 (MQ=255)                          gTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAAtata                                                                                                                                                                                                                                                              >  1:238162/1‑300 (MQ=255)                                aTTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACcgacg                                                                                                                                                                                                                                                        <  2:202940/300‑1 (MQ=255)                                        ctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGtttt                                                                                                                                                                                                                                                >  1:188251/1‑300 (MQ=255)                                        ctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCACGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGtttt                                                                                                                                                                                                                                                >  1:607824/1‑300 (MQ=255)                                          aCCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCNGCAGCCTGTTTCTGCCCTTCAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCANGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTCCCGTCCCCGCCATGTTACACATGGAAGGTCACGGCAAACTGCAATATACCGAGGAGGGTTTTct                                                                                                                                                                                                                                              >  2:560576/1‑299 (MQ=255)                                           ccgtgAGGCCCTATGAATTGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCATAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATTTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCg                                                                                                                                                                                                                                             <  2:14232/296‑1 (MQ=255)                                                        tGAATCACTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTGCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGAtg                                                                                                                                                                                                                                <  2:122718/300‑1 (MQ=255)                                                              gCTGGGAAAACATTCAGCGCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGt                                                                                                                                                                                                                          <  2:251910/300‑1 (MQ=255)                                                                    aaaaCATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTcc                                                                                                                                                                                                                    <  1:378515/300‑1 (MQ=255)                                                                    aaaaCATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTNCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTcc                                                                                                                                                                                                                    <  1:461818/300‑1 (MQ=255)                                                                           tcggttNACCCACGAAAACCGACTTTCGCCGCGTGCGTACTTTTTTTCATATGANTCTGTTGCGCAAGCGCGTACCTTTGCCCTCGAAACCAGCAGCCTGTTTCTGCACTTAAGCGGTCAGTGGAATTTCCACTTTTTGGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGTAAGGTCACGGCAAACTGCAATATACCGACTAACTTTTTCCGTGCCCCATCGATGTGCCGTTTGTCCCCAGCGa                                                                                                                                                                                                             <  2:574296/295‑1 (MQ=255)                                                                              gCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATaa                                                                                                                                                                                                          >  1:664914/1‑300 (MQ=255)                                                                               cTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTNAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCNCATCGATGTGCCGTTTGTCCCCAGCGATAAc                                                                                                                                                                                                         <  2:494467/300‑1 (MQ=255)                                                                                 cACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAAccc                                                                                                                                                                                                       <  2:296061/300‑1 (MQ=255)                                                                                 cACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCACGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCACAGCGATAAccc                                                                                                                                                                                                       >  1:432603/1‑300 (MQ=255)                                                                                   cacACGAAAACCGAATTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCATTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCAATGTGAAAAATGGAAGGTAACGGCAAACTGCAATGTACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCAGTTTGTCCCCAGCGATAACCCaa                                                                                                                                                                                                     >  2:296858/3‑300 (MQ=255)                                                                                     cACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCACAGCGATAACCCAAcc                                                                                                                                                                                                   >  2:218303/1‑300 (MQ=255)                                                                                      aCGAAAACCTACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCg                                                                                                                                                                                                  <  2:230726/300‑1 (MQ=255)                                                                                               gACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATc                                                                                                                                                                                         <  1:542230/300‑1 (MQ=255)                                                                                               gACTTGCGACGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCGAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGTGGGCATATTACCATCCCCGCCATTTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATc                                                                                                                                                                                         <  2:280359/300‑1 (MQ=255)                                                                                                 cTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCaa                                                                                                                                                                                       <  1:556753/300‑1 (MQ=255)                                                                                                 cTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATNTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCaa                                                                                                                                                                                       <  2:618804/300‑1 (MQ=255)                                                                                                   tGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACg                                                                                                                                                                                     >  1:563196/1‑300 (MQ=255)                                                                                                             gcgtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCAcc                                                                                                                                                                           <  1:342788/300‑1 (MQ=255)                                                                                                              cgtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCAccc                                                                                                                                                                          <  1:226125/300‑1 (MQ=255)                                                                                                              cgtACTNTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAANCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCAccc                                                                                                                                                                          <  2:575181/300‑1 (MQ=255)                                                                                                                         tCATATGATTCTGCTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGc                                                                                                                                                               <  1:645981/300‑1 (MQ=255)                                                                                                                               gATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAg                                                                                                                                                         <  2:286286/300‑1 (MQ=255)                                                                                                                                      ttGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAAc                                                                                                                                                  <  1:55080/300‑1 (MQ=255)                                                                                                                                        gcgcACGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCNGTCAGGGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGGAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGGGCCTATCAATGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAg                                                                                                                                                <  2:527209/300‑1 (MQ=255)                                                                                                                                        gcgcAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCNGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAg                                                                                                                                                <  2:526958/300‑1 (MQ=255)                                                                                                                                        gcgcAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCANCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAg                                                                                                                                                <  1:502740/300‑1 (MQ=255)                                                                                                                                        gcgcAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCCATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTCAACAg                                                                                                                                                >  1:551027/1‑300 (MQ=255)                                                                                                                                        gcgcAAGCGCGTACCTGTACCCGCGAAACCAGCAGCCTGTTTCTACCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTGCCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGNGGCATACTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAATGTATTTTCACTCTCAGCGACGGCTGGCAGGGTAAACAg                                                                                                                                                <  2:168012/300‑1 (MQ=255)                                                                                                                                                     ccTTTGCCCGCGAAACCAGCAGCCTGTTTCTNCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCANATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGAAGCTGATTAAAt                                                                                                                                   >  2:482877/1‑300 (MQ=255)                                                                                                                                                                          gcCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTAtt                                                                                                              >  1:399066/1‑300 (MQ=255)                                                                                                                                                                              gTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAANTGGAAGGTCACGGCAAACTGCAATATNCCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGa                                                                                                          >  2:479070/1‑300 (MQ=255)                                                                                                                                                                                    gCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTa                                                                                                    >  1:71684/1‑300 (MQ=255)                                                                                                                                                                                     cccTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTAt                                                                                                   >  2:241841/1‑300 (MQ=255)                                                                                                                                                                                       cTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGt                                                                                                 >  2:74012/1‑300 (MQ=255)                                                                                                                                                                                        ttAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTTGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGtt                                                                                                <  2:281140/300‑1 (MQ=255)                                                                                                                                                                                        ttAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCNTGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGtt                                                                                                <  2:609259/300‑1 (MQ=255)                                                                                                                                                                                        ttAAGCGGTCAGGGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGtt                                                                                                <  2:301481/300‑1 (MQ=255)                                                                                                                                                                                           aGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAAc                                                                                             <  1:704568/300‑1 (MQ=255)                                                                                                                                                                                                     ggAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGTGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTAtg                                                                                   <  1:417931/300‑1 (MQ=255)                                                                                                                                                                                                     ggAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTAtg                                                                                   <  1:623342/300‑1 (MQ=255)                                                                                                                                                                                                     ggAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTAtg                                                                                   <  2:597252/300‑1 (MQ=255)                                                                                                                                                                                                       aaTTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTAtgtg                                                                                 <  1:169014/300‑1 (MQ=255)                                                                                                                                                                                                         tttCCANTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTNATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCGTCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTggg                                                                               <  2:581981/300‑1 (MQ=255)                                                                                                                                                                                                         tttCCACTTTTTTTACCATCCGCTGCAAGTACCAGAAGCCTTCATCTCTTAGTTAATGGCTGACTGGGGGCATATTATCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTggg                                                                               <  2:62786/300‑1 (MQ=255)                                                                                                                                                                                                         tttCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGTGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTggg                                                                               <  2:338170/300‑1 (MQ=255)                                                                                                                                                                                                         tttCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACTTATTTTGAAGTCTATGTTAACGGTCAGTATGTggg                                                                               <  2:8548/300‑1 (MQ=255)                                                                                                                                                                                                         tttCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTggg                                                                               <  1:130158/300‑1 (MQ=255)                                                                                                                                                                                                                      gACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGc                                                                  <  2:361228/300‑1 (MQ=255)                                                                                                                                                                                                                                     gTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGAAGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGNCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCa                                                   >  2:617441/1‑300 (MQ=255)                                                                                                                                                                                                                                             aGCCTTCACCTCTTAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGa                                           >  1:201676/1‑300 (MQ=255)                                                                                                                                                                                                                                              gCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCNCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGAc                                          <  1:504511/300‑1 (MQ=255)                                                                                                                                                                                                                                              gCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGAc                                          >  1:277923/1‑300 (MQ=255)                                                                                                                                                                                                                                                    aCCTCTGAGTTAATGGCTGACTGGGGGCATATTAACGTCCACGACATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGGTGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTCGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGAACGCCGAGTTTGACATCAgc                                    >  2:141032/1‑300 (MQ=255)                                                                                                                                                                                                                                                     ccTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAgcg                                   >  1:422194/1‑300 (MQ=255)                                                                                                                                                                                                                                                      ctctGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAgcgc                                  >  1:576145/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGt                            >  2:412191/1‑300 (MQ=255)                                                                                                                                                                                                                                                                aTGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTaaa                        <  1:227197/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  ggCTGACTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAAc                      <  2:30577/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        cTGGGGGCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGa                <  1:533203/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATANCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCANTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCt          >  2:660743/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAAccg          >  2:890/1‑299 (MQ=255)                                                                                                                                                                                                                                                                              gCATATTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTAAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCt          >  2:380315/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                atcttACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCtgt        >  1:436172/4‑300 (MQ=255)                                                                                                                                                                                                                                                                                atatTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGTGACAACCtgt        >  2:429186/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  atTACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCtgttg      <  1:582353/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    tACCGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTtgtg    <  1:112300/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ccGTCCCCGCCATTTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTtgtgtg  <  1:59524/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ccGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCANGGTAAACAGACGCTGATTAAATTTGANGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTtgtgtg  <  2:470580/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ccGTCCCCGCCATGTGACAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTtgtgtg  <  1:256061/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                            ACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTG  >  NC_000913/3222579‑3223155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑