Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	277,277	G→A	R188H (CGT→CAT)	mmuM →	homocysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	277,277	0	G	A	100.0%	206.1 / NA	60	R188H (CGT→CAT)	mmuM	homocysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/42);  total (18/42)

GCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAGTGGCAGGCC  >  NC_000913/276982‑277572                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        gcgcTGCTTGACAAAAGCGTGGAGCTGTCGCGTAAAGCCCTTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGGCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCTTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATgcg                                                                                                                                                                                                                                                                                                     <  2:602943/300‑1 (MQ=255)        ttGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGt                                                                                                                                                                                                                                                                                              <  1:228395/300‑1 (MQ=255)                  cGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCAtt                                                                                                                                                                                                                                                                                    <  1:158937/300‑1 (MQ=255)                      gggCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAAAAAACCCGCAGGCGGGAACGCTTCTGGTGGTAGAATCCGTCGGGCCCTCCGGCGCGTATCTGGCGGGTGGCTCTGCATACCGTGGAGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCGTGCTGGATGCCGGGGCCGATCTACTGGCCTGCGAAACCCTGCCGCATTTTTCCGAGATCGAGGAGTTGGCCGAGTTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTTATTTAcc                                                                                                                                                                                                                                                                                >  2:368500/3‑300 (MQ=255)                      gcgCTGGCGCGTAAAGCACGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGCTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATCGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTaac                                                                                                                                                                                                                                                                                >  2:38097/3‑298 (MQ=255)                       aGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTTAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTTAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTAccc                                                                                                                                                                                                                                                                               <  2:198969/300‑1 (MQ=255)                       aGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTAccc                                                                                                                                                                                                                                                                               <  1:103978/300‑1 (MQ=255)                       aGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTAccc                                                                                                                                                                                                                                                                               <  2:677839/300‑1 (MQ=255)                       aGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCCTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTAccc                                                                                                                                                                                                                                                                               <  2:541233/300‑1 (MQ=255)                               cgTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGAc                                                                                                                                                                                                                                                                       <  2:449016/300‑1 (MQ=255)                                         gTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACAcc                                                                                                                                                                                                                                                             <  1:196936/300‑1 (MQ=255)                                              gCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTNTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGc                                                                                                                                                                                                                                                        <  1:465819/300‑1 (MQ=255)                                              gCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGc                                                                                                                                                                                                                                                        <  1:541861/300‑1 (MQ=255)                                                        cAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTAcgc                                                                                                                                                                                                                                              <  1:639517/300‑1 (MQ=255)                                                         agagAACCCGCAGTCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGTCGATTATCATTGTAGCGTTTAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGATGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTAcgcc                                                                                                                                                                                                                                             <  2:86860/300‑1 (MQ=255)                                                           agaACCCGCAGGCGGGGACGCTTCTGGTTGCGAGAGCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCGGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGAGCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTTAGGCGTTTGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTAcgccgc                                                                                                                                                                                                                                           <  2:362893/300‑1 (MQ=255)                                                           agaACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGACCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTAcgccgc                                                                                                                                                                                                                                           >  2:603324/1‑300 (MQ=255)                                                               cccGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCg                                                                                                                                                                                                                                       <  1:623589/300‑1 (MQ=255)                                                                  gCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGa                                                                                                                                                                                                                                    <  2:412928/300‑1 (MQ=255)                                                                     tgcgtgAACGCCTCTGGTGGCTGGATCCTTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGTCGATTATCATTGTAGCGTTGAGGCATTTCAGTCGTTTCATCGCCCGCGCGTGTAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGt                                                                                                                                                                                                                                 <  2:387995/295‑1 (MQ=255)                                                                        gtgAACGCTTCTGGTGGCGGGATCCTTCGGGCCTTACGGCGCGTATTTGGCGGATGGCTATGAATACCTTGGCGATTATCATTGTAGCGTTTAGGCATTTCATGCATTTCATCGCCCGCGCTTGTAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCTAAACCCTGCCGAATTTTTCCTAGATTGAGGCGTTGGCCGAACTGTTGACCGCACATCCGCATGCGCGGGCGTGGGTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCACTGCGTGACGTGgt                                                                                                                                                                                                                              <  2:123791/298‑1 (MQ=255)                                                                                       ggCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTTAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCggg                                                                                                                                                                                                               <  1:262877/300‑1 (MQ=255)                                                                                                      gCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAggtggt                                                                                                                                                                                                >  1:518703/1‑300 (MQ=255)                                                                                                         ttACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGGAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGgc                                                                                                                                                                                             <  2:326463/300‑1 (MQ=255)                                                                                                              gcgcGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATTATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTACGAGATTGAGGCGTTGGCCGAGNTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAg                                                                                                                                                                                        >  2:156597/1‑300 (MQ=255)                                                                                                                        ggCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTg                                                                                                                                                                              <  1:605865/300‑1 (MQ=255)                                                                                                                           ggATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTAt                                                                                                                                                                           <  1:311178/300‑1 (MQ=255)                                                                                                                                gCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTgcgc                                                                                                                                                                      >  1:191618/1‑300 (MQ=255)                                                                                                                                gCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCAGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCCGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTgcgc                                                                                                                                                                      >  2:8937/1‑300 (MQ=255)                                                                                                                                gCTCTAAATACCGTGGCGATTATCAGGGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTgcgc                                                                                                                                                                      <  2:14849/300‑1 (MQ=255)                                                                                                                                  tctGAATACCGTGGCGATTATTATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCCGAGCGACGGTACGCAGCTGCGTAAAGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAAGCATCAACTGTATTGCGCTg                                                                                                                                                                    >  2:84050/1‑300 (MQ=255)                                                                                                                                     gAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGaa                                                                                                                                                                 >  2:594159/1‑300 (MQ=255)                                                                                                                                                    tATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCNCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCAANGCTGCg                                                                                                                                                  >  2:578418/1‑300 (MQ=255)                                                                                                                                                     aTCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGt                                                                                                                                                 <  1:673263/300‑1 (MQ=255)                                                                                                                                                        aTTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTgc                                                                                                                                              >  1:97127/1‑300 (MQ=255)                                                                                                                                                         ttGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTgca                                                                                                                                             >  1:207649/1‑300 (MQ=255)                                                                                                                                                          tGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTgcag                                                                                                                                            <  1:177687/300‑1 (MQ=255)                                                                                                                                                                       cATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCAGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTACGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCCAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAACATTTACACGGtt                                                                                                                               >  2:147736/1‑300 (MQ=255)                                                                                                                                                                           tCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTTAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCNACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCNCCGCTGCGTTGCAGCATTTACACGGTTTAAc                                                                                                                           <  2:504933/300‑1 (MQ=255)                                                                                                                                                                             aGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCTGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCTTGACGTGGTTGCGTTGCTTGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACgg                                                                                                                         <  1:594464/300‑1 (MQ=255)                                                                                                                                                                             aGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACgg                                                                                                                         <  1:194996/300‑1 (MQ=255)                                                                                                                                                                             aGGCGTTTCATCGCCCGCGCGTGGAAGCCTGGCTGGATGCCTGGTCCGATCTGCTTGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGTCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTCTAACgg                                                                                                                         <  2:65204/300‑1 (MQ=255)                                                                                                                                                                                cGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGtgc                                                                                                                      <  1:340957/300‑1 (MQ=255)                                                                                                                                                                                   ttCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGtgctgc                                                                                                                   >  1:164021/1‑300 (MQ=255)                                                                                                                                                                                                        ccTTGCGGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGATGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACt                                                                                              <  1:362143/300‑1 (MQ=255)                                                                                                                                                                                                                   gCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCAt                                                                                   <  1:23366/300‑1 (MQ=255)                                                                                                                                                                                                                        ggCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGa                                                                              <  1:227950/300‑1 (MQ=255)                                                                                                                                                                                                                        ggCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACAACTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGa                                                                              <  1:368764/300‑1 (MQ=255)                                                                                                                                                                                                                        ggCCAATCGGCTGGCCTGCGAAACCCTACCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCACATCCGCATGCGCGGGCTTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGa                                                                              <  2:71493/300‑1 (MQ=255)                                                                                                                                                                                                                           cGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGc                                                                           <  1:183222/300‑1 (MQ=255)                                                                                                                                                                                                                             aTCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCg                                                                         <  1:44738/300‑1 (MQ=255)                                                                                                                                                                                                                              tCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTNAACGGTGCTGCCGCTGGTGGTGTATCCGAACCCGGGCGAGCATTACGNTGcagt                                                                        >  2:579563/1‑297 (MQ=255)                                                                                                                                                                                                                                  ctgGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGc                                                                    <  1:632053/300‑1 (MQ=255)                                                                                                                                                                                                                                         gCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGAACGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGCACTCGGGCGAGCATTAAGATGCCGTGAGCAAAACCt                                                             >  2:626429/1‑300 (MQ=255)                                                                                                                                                                                                                                             agcccTGCCGAATTTTTCCGAGAGTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCACATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTNGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCATGAGCAAAACCTGGca                                                         <  2:165403/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGTGGATTAt                    >  1:280375/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAACGAACACCCGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGACGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGATGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAAAGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCNGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCNGATTAt                    >  2:587251/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGNGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCt                  <  1:471114/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           aCCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAg           <  1:612399/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    ccGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAGTGGCAGGcc  <  1:101170/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        GCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAGTGGCAGGCC  >  NC_000913/276982‑277572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑