Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,941,835	G→A	Q245* (CAA→TAA)	gcvA ←	DNA‑binding transcriptional dual regulator GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,941,835	0	G	A	100.0%	305.1 / NA	89	Q245* (CAA→TAA)	gcvA	DNA‑binding transcriptional dual regulator GcvA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/42);  total (47/42)

GGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCACCCGTAGCCCCGGCCAGTTGCCCCGACCATAA  >  NC_000913/2941539‑2942130                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        ggTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTacgc                                                                                                                                                                                                                                                                                                      <  1:437874/300‑1 (MQ=255)  gTTTTACTTAACACATGCGCGCCAAAAGCGCNCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACNAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCATCGATTTCAGATTACGcc                                                                                                                                                                                                                                                                                                     >  2:490773/1‑300 (MQ=255)       aCTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGTGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCAc                                                                                                                                                                                                                                                                                                >  2:633845/1‑300 (MQ=255)       aCTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGNGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTNCCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCAc                                                                                                                                                                                                                                                                                                >  2:563332/1‑300 (MQ=255)       aCTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCAc                                                                                                                                                                                                                                                                                                >  1:173969/1‑300 (MQ=255)       aCTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCAAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTACGGCCTCGATTTCAGATTACGCCATCAc                                                                                                                                                                                                                                                                                                >  1:536293/1‑300 (MQ=255)          tAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACATCCAGCCTCGATTTCAGATTACGCCATCAcatt                                                                                                                                                                                                                                                                                             >  1:20745/1‑297 (MQ=255)          tAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGAAGTCCGGCCTCGATTTCAGATTACGCCATCACGtt                                                                                                                                                                                                                                                                                             >  2:275200/1‑300 (MQ=255)            acacATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTAt                                                                                                                                                                                                                                                                                           >  2:634922/1‑300 (MQ=255)                 tGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGcc                                                                                                                                                                                                                                                                                      <  1:7381/300‑1 (MQ=255)                     cgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAgcg                                                                                                                                                                                                                                                                                  >  2:682473/1‑300 (MQ=255)                           aaGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGTTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAANTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTc                                                                                                                                                                                                                                                                            <  1:484452/300‑1 (MQ=255)                           aaGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTc                                                                                                                                                                                                                                                                            <  1:67108/300‑1 (MQ=255)                                 ccAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGcc                                                                                                                                                                                                                                                                      <  1:670226/300‑1 (MQ=255)                                           aaaaaTGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACTACGGTTCATGGTCGTACCCTACTGAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTGACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGTCCTCGATTTCAGATTACGCCATCACGTGATTTGCCAGCGCCACTCCCTGCCCGTGGAGAGc                                                                                                                                                                                                                                                            <  1:89633/300‑1 (MQ=255)                                           aaaaaTGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAATCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGc                                                                                                                                                                                                                                                            <  2:269460/300‑1 (MQ=255)                                           aaaaaTGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGc                                                                                                                                                                                                                                                            <  2:680038/300‑1 (MQ=255)                                                    gCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGNGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTNTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCACCACGTTATTTGCCAGCGCCACTCCCTGCACGTGGATAGCCGCTTgcag                                                                                                                                                                                                                                                   >  2:556520/1‑300 (MQ=255)                                                               cggcgAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGgcgc                                                                                                                                                                                                                                        <  1:298406/300‑1 (MQ=255)                                                                            cATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAAt                                                                                                                                                                                                                           <  1:53698/300‑1 (MQ=255)                                                                                 aaCGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGcc                                                                                                                                                                                                                      <  2:536184/300‑1 (MQ=255)                                                                                        ggTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTg                                                                                                                                                                                                               >  2:378238/1‑300 (MQ=255)                                                                                        ggTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTg                                                                                                                                                                                                               >  1:702709/1‑300 (MQ=255)                                                                                         gTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCAAGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGACCTTGCTGa                                                                                                                                                                                                              >  2:130976/1‑300 (MQ=255)                                                                                             tGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGt                                                                                                                                                                                                          >  1:622616/1‑300 (MQ=255)                                                                                               gTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAGGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTCACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTNGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTg                                                                                                                                                                                                        >  1:463950/1‑300 (MQ=255)                                                                                               gTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACNAACCAGATAAAAAGCATTTTTACTGANCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTg                                                                                                                                                                                                        >  2:465775/1‑300 (MQ=255)                                                                                               gTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATNGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGNATGTTg                                                                                                                                                                                                        >  2:580899/1‑300 (MQ=255)                                                                                               gTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTg                                                                                                                                                                                                        >  2:453547/1‑300 (MQ=255)                                                                                               gTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTg                                                                                                                                                                                                        >  1:56857/1‑300 (MQ=255)                                                                                               gTCGTACCCTAAGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGACAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTg                                                                                                                                                                                                        >  2:672782/1‑300 (MQ=255)                                                                                                       cTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGAtt                                                                                                                                                                                                <  1:547451/300‑1 (MQ=255)                                                                                                       cTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTAAGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGAtt                                                                                                                                                                                                >  2:145870/1‑300 (MQ=255)                                                                                                         aCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTa                                                                                                                                                                                              >  1:667859/1‑300 (MQ=255)                                                                                                         aCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTACTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGCTTTa                                                                                                                                                                                              >  2:147981/1‑300 (MQ=255)                                                                                                            tAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCATGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATGAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAAcc                                                                                                                                                                                           <  2:187922/300‑1 (MQ=255)                                                                                                             agattattGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCTAAAGGTGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAAccc                                                                                                                                                                                          <  2:92368/298‑1 (MQ=255)                                                                                                             aaaTTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCTGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTNCTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAAccc                                                                                                                                                                                          <  2:167420/300‑1 (MQ=255)                                                                                                             aaaTTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTNCTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAAccc                                                                                                                                                                                          <  2:167461/300‑1 (MQ=255)                                                                                                             aaaTTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATCTGATTTAAccc                                                                                                                                                                                          >  1:304262/1‑300 (MQ=255)                                                                                                             aaaTTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAAccc                                                                                                                                                                                          <  1:171596/300‑1 (MQ=255)                                                                                                                ttattGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCaa                                                                                                                                                                                       >  2:411844/1‑300 (MQ=255)                                                                                                                        ctgcgagatgtgtataagagacaggTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCggg                                                                                                                                                                               >  1:602598/25‑300 (MQ=255)                                                                                                                        cctAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAAATGTCggg                                                                                                                                                                               >  1:370979/3‑300 (MQ=255)                                                                                                                         aTAACGNAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGNCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAACCAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGACCGTTGATATGATTTAACCCCAACTGTCGGGt                                                                                                                                                                              <  2:587275/300‑1 (MQ=255)                                                                                                                               aaaGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTTGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTNAACGGGCAAACAAGACGTCCTGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCTTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGt                                                                                                                                                                        <  2:163000/300‑1 (MQ=255)                                                                                                                                aaGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTc                                                                                                                                                                       <  1:248477/300‑1 (MQ=255)                                                                                                                                    ggAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGcc                                                                                                                                                                   <  1:82488/300‑1 (MQ=255)                                                                                                                                    ggAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGAGTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGcc                                                                                                                                                                   <  2:683240/300‑1 (MQ=255)                                                                                                                                                gTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCANACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTNCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGGTATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGcgcg                                                                                                                                                       >  2:557712/1‑300 (MQ=255)                                                                                                                                                gTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGcgcg                                                                                                                                                       >  1:707856/1‑300 (MQ=255)                                                                                                                                                           gCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGt                                                                                                                                            <  1:295196/300‑1 (MQ=255)                                                                                                                                                              ttCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGtaat                                                                                                                                         <  1:142227/300‑1 (MQ=255)                                                                                                                                                                cGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCNTGACAAACCAGATAAAAAGCATTTTTAGTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGtaataa                                                                                                                                       <  2:514944/300‑1 (MQ=255)                                                                                                                                                                cGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATGACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGtaataa                                                                                                                                       <  1:237582/300‑1 (MQ=255)                                                                                                                                                                cGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGtaataa                                                                                                                                       <  1:531831/300‑1 (MQ=255)                                                                                                                                                                   cAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAATACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGt                                                                                                                                    <  2:265408/300‑1 (MQ=255)                                                                                                                                                                             tGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGcc                                                                                                                          <  1:131466/300‑1 (MQ=255)                                                                                                                                                                                   ctgcgagatgtgtataagagacaggCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAACTTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATTTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTCGCCAGATCt                                                                                                                    >  1:642206/25‑300 (MQ=255)                                                                                                                                                                                       cggcTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCg                                                                                                                >  2:535051/1‑300 (MQ=255)                                                                                                                                                                                                 aCCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATCTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCAAGATCTTCCGGTGTCTTCaa                                                                                                      >  2:268301/1‑300 (MQ=255)                                                                                                                                                                                                    cAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGTGCAAACAATACGTCCTGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAGTAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAggg                                                                                                   <  2:73129/300‑1 (MQ=255)                                                                                                                                                                                                      gTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGtt                                                                                                 >  1:602205/1‑300 (MQ=255)                                                                                                                                                                                                      gTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCAAGATCTTCCGGTGTCTTCAAGGGtt                                                                                                 >  1:50213/1‑300 (MQ=255)                                                                                                                                                                                                      gTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACATCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTANGGCTAAAAATTGGACATTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAGGCATCATGTAATAACATATGTTTAGCCAGATCttaaggtgctttcaaaggtt                                                                                                 >  2:154095/1‑282 (MQ=255)                                                                                                                                                                                                         cTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGttttt                                                                                              >  1:112376/1‑300 (MQ=255)                                                                                                                                                                                                           gCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTTACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCg                                                                                            >  1:597709/1‑300 (MQ=255)                                                                                                                                                                                                           gCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCg                                                                                            >  2:408507/1‑300 (MQ=255)                                                                                                                                                                                                                 cTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTc                                                                                      >  1:457109/1‑300 (MQ=255)                                                                                                                                                                                                                   gTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATATCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTANTAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTcag                                                                                    >  2:515952/1‑300 (MQ=255)                                                                                                                                                                                                                   gTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGTATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTcag                                                                                    >  1:1569/1‑300 (MQ=255)                                                                                                                                                                                                                      aTGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAATACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCGTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTcagcag                                                                                 <  1:559470/300‑1 (MQ=255)                                                                                                                                                                                                                      aTGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTcagcag                                                                                 <  1:198917/300‑1 (MQ=255)                                                                                                                                                                                                                            aaCCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAgcgg                                                                           <  1:712491/300‑1 (MQ=255)                                                                                                                                                                                                                                                tACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCaataaat                                                       <  1:448892/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTNCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATANATATTc                                                   >  2:570049/1‑300 (MQ=255)                                                                                                                                                                                                                                                      ccAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCgg                                                 >  1:408451/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGt                                              >  1:192685/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     aCGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCa                                  <  2:322779/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        ggCAAACAAGACGTCCGGCCTCGATTTCAGANTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGNACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCAccc                               >  2:485518/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            aaCAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCACCCGTAg                           >  1:282198/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              cAAGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCACCCGTAGcc                         <  2:367451/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              cAAGACGTCCGGCCTCGATTTCAGATTACACCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTACGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCACCCGTAGcc                         <  2:337233/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aaGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCNCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCACCCGTAGccc                        <  1:501474/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aaGACGTCCGGCCTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCACCCGTAGccc                        <  1:390303/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           cTCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTcctgtctcttatacacatctcgcag            <  1:224907/300‑24 (MQ=255)                                                                                                                                                                                                                                                                                            tCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTTCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCACCCGTAGCCCCGGCCAGTTGccc           <  2:147625/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            tCGATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCACCCGTAGCCCCGGCCAGTTGccc           <  1:382155/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              gATTTCAGATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCACCCGTAGCCCCGGCCAGTTGCCCCg         <  1:629333/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     gATTACGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCACCCGTAGCCCCGGCCAGTTGCCCCGACCATaa  >  1:56980/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        GGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACGTATGTTTAGCCAGATCTTCCGGTGTCTTCAAGGGTTTTTCGCCAGTCAGCAGTAGCGGCGAACACACCGGCAATAAATATTCGGCGTACAGTTTTTCCACCCGTAGCCCCGGCCAGTTGCCCCGACCATAA  >  NC_000913/2941539‑2942130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑