| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,001,282 | G→A | L170L (CTA→TTA) | fliA ← | RNA polymerase sigma factor FliA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,001,282 | 0 | G | A | 98.4% | 212.5 / NA | 64 | L170L (CTA→TTA) | fliA | RNA polymerase sigma factor FliA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (30/33); minor base C (1/0); total (31/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAGCGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACGGCTGCGAAGTTCATCCAGCATAGCGCCACGGATACGCTGCAC > NC_000913/2000989‑2001576 | gCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCAGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTACAGTAGTTGTTGTAACGGGtt > 2:229428/1‑300 (MQ=255) aTGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTtctc < 1:420904/300‑1 (MQ=255) ctttgccggggctgcACCATCATTAAGAACTCCTGGTAGTCGAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACCGACTGACCCGCGGTTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTTAGCTCTTCCTGGT‑AATAAAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGGTGTAACGGGTGGTCTCGCtgatga < 2:68540/290‑1 (MQ=255) tttCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGGAGATTACTGTCTAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGatc > 2:92676/1‑300 (MQ=255) tttCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGatc < 1:529042/300‑1 (MQ=255) ttCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGANCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AANAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGatca > 2:548778/1‑300 (MQ=255) ggTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAg > 1:246058/1‑300 (MQ=255) gTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGt > 2:197433/1‑300 (MQ=255) tgtaCCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCTGCATTTACTTACGTTATAACGTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTTGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAAcc < 1:305564/297‑1 (MQ=255) tgcACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAAcc < 1:528924/300‑1 (MQ=255) gcACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCa > 2:348199/1‑300 (MQ=255) gcACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCa > 1:543518/1‑300 (MQ=255) atTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTTGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATg < 2:75625/300‑1 (MQ=255) ttAAGAACTCCTGGTAGTCAAATTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGc < 1:134423/300‑1 (MQ=255) ttAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTTGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGaggc < 1:403457/300‑4 (MQ=255) aCTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATACCTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCCGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTACCGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGCTGCTATCga > 2:355556/1‑299 (MQ=255) gTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGctc < 1:27521/300‑1 (MQ=255) gTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCNGGT‑AATAGAGGGTTAATACCAGTTTTNCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGctc < 2:457731/300‑1 (MQ=255) aaGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCgcgc > 1:638823/1‑300 (MQ=255) gTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATc < 1:319892/300‑1 (MQ=255) tACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCTCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGATGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGagaag < 1:187407/300‑1 (MQ=255) cGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAgctgg > 1:610097/1‑300 (MQ=255) ttATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAgctggct < 1:645851/300‑1 (MQ=255) aaCTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGTCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTa > 1:658268/1‑300 (MQ=255) tACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGTAGAAGAGCTGGCTGTTATTgg < 2:271300/300‑1 (MQ=255) aCCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTACTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTACATCAACCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGgt > 2:265473/1‑300 (MQ=255) ccGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTNTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCg > 2:617582/1‑300 (MQ=255) ccGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCg < 2:4974/300‑1 (MQ=255) gCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCa > 1:601492/1‑300 (MQ=255) ggCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAatat < 1:193389/300‑1 (MQ=255) tgtgTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGa < 1:432853/300‑1 (MQ=255) gtgtAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGAt > 1:458875/1‑300 (MQ=255) gtgtAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGAt > 2:439556/1‑300 (MQ=255) gtAACTGACTGACCCGCGATTCCCCGACCTACAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATcc > 2:73147/1‑300 (MQ=255) gactgacCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAAc < 1:653797/300‑1 (MQ=255) actgacCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCC‑GGTGAATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACg < 2:682856/300‑1 (MQ=255) actgacCCGCGATTCCCCGACCTCCAGCACCGCGCCAACTTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCAATAATCGGCAATATCGATCCCTAAACg < 2:180634/300‑1 (MQ=255) acCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTcc > 1:435267/1‑300 (MQ=255) acCCGCGATTCCCCGAACTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTcc > 1:434940/1‑300 (MQ=255) aCCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTAGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTcc > 2:271106/1‑300 (MQ=255) cTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCNGTAGTTGTTGTAACGGGTTTTCTCGCNGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTAGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGATACCTCAGTTTCCGt > 2:474725/1‑300 (MQ=255) cTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGt > 1:586173/1‑300 (MQ=255) cTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCATAAACGTTCCGCTACCTCAGTTTCCGt > 1:199311/1‑300 (MQ=255) aTTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAg > 1:579720/1‑300 (MQ=255) ccTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTg < 1:389488/300‑1 (MQ=255) tGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCCGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGcc > 1:568338/1‑300 (MQ=255) tGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGcc < 1:349922/300‑1 (MQ=255) ggTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCAACCGCTGGCGCAGATTAATGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTACGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTc > 2:52779/1‑300 (MQ=255) gTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCa > 1:628473/1‑300 (MQ=255) gTTAATACCAGTTTTTCGCGCTCCGGCAACGNTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGANGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCa > 2:484936/1‑300 (MQ=255) gcTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTNATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGc < 2:169619/300‑1 (MQ=255) cGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTAACGCGCGTTGCGTCGCACGCTgcg > 2:30534/1‑300 (MQ=255) aaCGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTTTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATNATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCANTTCACGCGCGTTGCGTCGCACGCTGCGCGGc < 2:491479/300‑1 (MQ=255) cGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCAc < 1:30585/300‑1 (MQ=255) cGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGt < 1:45198/300‑1 (MQ=255) aTGGCTTCCATCACCCGCTGGTGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTNCGCGCCACTCATCGTAGTAGAAGAGCTGGCTGTTATTGGTGTTGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCa < 2:617411/300‑1 (MQ=255) aTGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCTTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCa < 1:211157/300‑1 (MQ=255) aTGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCACTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCa < 2:418757/300‑1 (MQ=255) gCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACgg > 1:290181/1‑300 (MQ=255) cTTCCATCACCCGCTGGCGCAGATTACTGGCCAGTAGTTGTTGTAACGGTTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACGGc < 1:349757/300‑1 (MQ=255) ttACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACGGCTGCGAAGTTCATCCAGCATAgcg > 1:65807/1‑300 (MQ=255) tACTGTCCAGTAGTTGTTGTAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTNTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGACCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACGGCTGCGAAGTTCATCCAGCATAgcgc < 1:490068/300‑1 (MQ=255) ttgtAACGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACGGCTGCGAAGTTCATCCAGCATAGCGCCACGGATACGCTGCAc < 1:55444/300‑1 (MQ=255) ttgtAACGGGTTTTCTCGCGGAGGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGTAGAAGAGCTGGCTGTTAGTGGTGTCGAGCAACATTTGGCGATAATCGGCAATNTCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACGGCTGCGAAGTTCATCCAGCATAGCGCCACGGATACGCTGCAc < 2:168435/300‑1 (MQ=255) | GCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGT‑AATAGAGGGTTAATACCAGTTTTTCGCGCTCCGGCAACGTTTCGATGGCTTCCATCACCCGCTGGCGCAGATTACTGTCCAGTAGTTGTTGTAGCGGGTTTTCTCGCTGATGATCATCAGTAACCAGTTCGATGCTATCGCCGTGCTCTTCGCGCCACTCATCGTAGGAGAAGAGCTGGCTGTTATTGGTGTCGAGCAACATTTGGCGATAATCGGCAATATCGATCCCTAAACGTTCCGCTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACGGCTGCGAAGTTCATCCAGCATAGCGCCACGGATACGCTGCAC > NC_000913/2000989‑2001576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |