Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,919,413	G→A	G635E (GGG→GAG)	letB →	lipophilic envelope spanning tunnel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,919,413	0	G	A	100.0%	190.2 / NA	55	G635E (GGG→GAG)	letB	lipophilic envelope spanning tunnel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/28);  total (27/28)

TGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCATCTTGATACTATCCTCCAGCCGTATATCAACGTCGAACCAGGCCGGGGCAATCC  >  NC_000913/1919123‑1919692                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                        tGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTAc                                                                                                                                                                                                                                                                                <  1:593117/300‑1 (MQ=255)   aaGTTGGTGAATGGATTACCATGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGc                                                                                                                                                                                                                                                                              <  2:533500/300‑1 (MQ=255)    aGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCt                                                                                                                                                                                                                                                                             <  1:390628/300‑1 (MQ=255)    aGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTAGCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCt                                                                                                                                                                                                                                                                             <  1:390609/300‑1 (MQ=255)           gAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATC‑ATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTNGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCtt                                                                                                                                                                                                                                                                     >  1:491676/1‑300 (MQ=255)             aGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCttt                                                                                                                                                                                                                                                                    >  1:582897/1‑300 (MQ=255)             aGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCAGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCttt                                                                                                                                                                                                                                                                    >  1:583014/1‑300 (MQ=255)              gTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTc                                                                                                                                                                                                                                                                   >  1:662250/1‑300 (MQ=255)                                 gAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCgg                                                                                                                                                                                                                                                >  2:689730/1‑300 (MQ=255)                                 gAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCgg                                                                                                                                                                                                                                                >  1:336095/1‑300 (MQ=255)                                  aGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCNTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCAGGAAAACNGGCGGt                                                                                                                                                                                                                                               >  2:584902/1‑300 (MQ=255)                                                                  aGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGTGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGTCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGata                                                                                                                                                                                                               <  2:684294/300‑1 (MQ=255)                                                                                 aCCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGNGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGTAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGa                                                                                                                                                                                                >  2:149928/1‑300 (MQ=255)                                                                                 aCCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGNGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGa                                                                                                                                                                                                >  2:149829/1‑300 (MQ=255)                                                                                           cAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTACGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCt                                                                                                                                                                                      >  1:354645/1‑300 (MQ=255)                                                                                            aGCAATAGCGTGTTCTGTGCAGAAGGCGGGTCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTg                                                                                                                                                                                     <  1:626618/300‑1 (MQ=255)                                                                                             gCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGTGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGATGGATCTGa                                                                                                                                                                                    >  2:128059/1‑300 (MQ=255)                                                                                             gCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGa                                                                                                                                                                                    >  2:299044/1‑300 (MQ=255)                                                                                             gCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTACAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCAAATTCTCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGa                                                                                                                                                                                    >  2:109950/1‑300 (MQ=255)                                                                                               aaTAGCGCGTTCTGGGCAGAAGGCGATTCGAAAGTTCATCTAAATGGTATTGGTCTGACTGTACGGGCATCCCCACTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGAtt                                                                                                                                                                                  <  1:454008/300‑1 (MQ=255)                                                                                                                aGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACATGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAATGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAAGGAAGt                                                                                                                                                                 <  2:546285/300‑1 (MQ=255)                                                                                                                       ggggCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCa                                                                                                                                                          <  1:301167/300‑1 (MQ=255)                                                                                                                            gAAAGTTCATCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGTGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGACTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACACTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAGGGc                                                                                                                                                     <  2:84202/300‑1 (MQ=255)                                                                                                                            gAAAGTTCAGCTGAATGGTAGTGTTCTTACCGTACAGGCATCCCCACTATCCAGAGAATTAAAGCGAGCCATTAGCGTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGc                                                                                                                                                     <  1:252466/300‑1 (MQ=255)                                                                                                                            gAAAGTCCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGGAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTTGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGAGTACCGCACGCAATGAAGTACAGGCAAAGGc                                                                                                                                                     <  2:600878/300‑1 (MQ=255)                                                                                                                              aaGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCgg                                                                                                                                                   <  1:654404/300‑1 (MQ=255)                                                                                                                                 ttCAGCTGAATGGTAGTGGTCTTACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAATGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTAc                                                                                                                                                <  2:386534/300‑1 (MQ=255)                                                                                                                                                        aCCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGAcc                                                                                                                         <  2:445300/300‑1 (MQ=255)                                                                                                                                                           gTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCACTTCTCTCTCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCAAGACCttt                                                                                                                      >  2:217801/1‑300 (MQ=255)                                                                                                                                                           gTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTAAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCttt                                                                                                                      >  2:6573/1‑300 (MQ=255)                                                                                                                                                                       ccGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCggtggt                                                                                                          >  2:236792/1‑300 (MQ=255)                                                                                                                                                                           tctcCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATTAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTAcgc                                                                                                      <  2:29465/300‑1 (MQ=255)                                                                                                                                                                            ctcCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCGGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTAcgcg                                                                                                     >  1:532387/1‑300 (MQ=255)                                                                                                                                                                            ctcCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTAcgcg                                                                                                     >  1:566458/1‑300 (MQ=255)                                                                                                                                                                             tcCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACgcgc                                                                                                    <  1:667442/300‑1 (MQ=255)                                                                                                                                                                                   gCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGCAGTACAGGCAAAGGCGGTACTTTAACCGGAATATGTCCAGACCTTTGCTTGCGGTGGTACGCGCTtctcc                                                                                              >  2:68727/1‑299 (MQ=255)                                                                                                                                                                                   gCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCa                                                                                              >  1:302354/1‑300 (MQ=255)                                                                                                                                                                                   gCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCa                                                                                              >  2:666197/1‑300 (MQ=255)                                                                                                                                                                                       tAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTgg                                                                                          <  1:113223/300‑1 (MQ=255)                                                                                                                                                                                       gaaaGGGAGCCATTATCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGTGTAAAGGCGAGAAGCGTATTATGTATGCGTCCGAAACAGCGGCCCTTGCGGTTGGCGAGCAGACTACGCGGCACGCTTTCGAGGCAGGAAAACTGGCGGTCGGGGTGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTGACCCGGAATATGGCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTgg                                                                                          <  2:282586/299‑1 (MQ=255)                                                                                                                                                                                       caaaGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAATTCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTgg                                                                                          <  1:214383/299‑1 (MQ=255)                                                                                                                                                                                              agtcattgtcTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCNTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGTTATTGATNTCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACAcc                                                                                   <  2:556288/291‑1 (MQ=255)                                                                                                                                                                                                 cATTAGCTTCGACAACCTCAGCGGTGCCAGCNCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATNCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCa                                                                                >  2:489094/1‑300 (MQ=255)                                                                                                                                                                                                                        ttGCCAGCACCCGTCAGCGTAAATGCGATAAGCATATTCTGTATGCTTACGAAACAGCTACCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAATGGGCGGTCTGGAGGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATGACCGCACGCAATGAAGGACAGGCAAAGGTGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGc                                                         <  2:67287/299‑1 (MQ=255)                                                                                                                                                                                                                          tgggctcggagatgtgtataagagacagaGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCAt                                                       <  1:413320/272‑1 (MQ=255)                                                                                                                                                                                                                                  cggagtgtgtataagagacaggCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCATCTTGATAc                                               <  1:657700/279‑1 (MQ=255)                                                                                                                                                                                                                                  cAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGNGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACNCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCATCTTGATAc                                               <  2:646908/300‑1 (MQ=255)                                                                                                                                                                                                                                        gCGTAAAGGCGATAAGCGTATTCTGTATGCTTACGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCATCTTGATACTAtcct                                         >  1:81594/1‑300 (MQ=255)                                                                                                                                                                                                                                               ggCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCCTCTCCGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCATCTTGATACTATCCTCCAGCCg                                  >  2:245773/1‑300 (MQ=255)                                                                                                                                                                                                                                                    tAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATNTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCATCTTGATACTATCCTCCAGCCGTatat                             <  1:497264/300‑1 (MQ=255)                                                                                                                                                                                                                                                      aGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCATCtgtctcttatacacatctcgcagggga                           >  2:407798/1‑274 (MQ=255)                                                                                                                                                                                                                                                       gCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCNTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCNAATTTCGGCAGCTGGCGTTGAGCATCTTGATAcctgtctcttatacacatctcg                          >  2:645321/1‑279 (MQ=255)                                                                                                                                                                                                                                                              cTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTAGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCATCTTGATACTATCCTACAGCCGTATATCAACGTCGaa                   >  2:232040/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gCTTCCGAAACAGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCATCTTGATACTATCCTCCAGCCGTATATCAACGTCGAACCAGGc             <  1:122817/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aGCGGCCCGTGCGGTTGGCGAGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCATCTTGATACTATCCTCCAGCCGTATATCAACGTCGAACCAGGCCGGGGCAATcc  <  1:344710/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                        TGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGAGCATCTTGATACTATCCTCCAGCCGTATATCAACGTCGAACCAGGCCGGGGCAATCC  >  NC_000913/1919123‑1919692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑