Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,684,231	G→A	intergenic (+48/‑28)	rstB → / → tus	sensory histidine kinase RstB/DNA replication terminus site‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,684,231	0	G	A	100.0%	184.1 / NA	54	intergenic (+48/‑28)	rstB/tus	sensory histidine kinase RstB/DNA replication terminus site‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/25);  total (29/25)

GGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGC  >  NC_000913/1683960‑1684516                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                              ggATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAAtatta                                                                                                                                                                                                                                                                   >  1:395304/1‑300 (MQ=255)    ttGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATgg                                                                                                                                                                                                                                                                <  1:333715/300‑1 (MQ=255)                        cATATCTTTGAACCTTTTTTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGTGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGAc                                                                                                                                                                                                                                            <  1:188789/300‑1 (MQ=255)                        cATATCTTTGAACCTTTTGTTCGCCTCGATCCCGGCCGGGATCGCTCAACCGGCTGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTNCTCGTTTAGCTGTCCGTTATGGCATANCATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGAc                                                                                                                                                                                                                                            <  2:471390/300‑1 (MQ=255)                        cATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGAc                                                                                                                                                                                                                                            <  1:676176/300‑1 (MQ=255)                        cATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCACTCAACCGGCNGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGAc                                                                                                                                                                                                                                            <  2:522203/300‑1 (MQ=255)                                   aCCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCAGTGGCAATGGGCGGTACGGTTAATTTTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAacac                                                                                                                                                                                                                                 <  2:415302/300‑1 (MQ=255)                                   aCCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAacac                                                                                                                                                                                                                                 <  2:410836/300‑1 (MQ=255)                                      ttttGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTAc                                                                                                                                                                                                                              <  1:414495/300‑1 (MQ=255)                                        ttGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCt                                                                                                                                                                                                                            <  1:216634/300‑1 (MQ=255)                                                            cGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTTTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAagag                                                                                                                                                                                                        <  2:666067/300‑1 (MQ=255)                                                                       aaCCGGCGGCTGCGGGCTGGGGTTGGCAATTGTCCACTCTATAGCACTGGCAATGGTCGGTACGGTTAATTGTGACACCAGCGAACTGGTTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATAtt                                                                                                                                                                                             <  2:318641/300‑1 (MQ=255)                                                                       aaCCGGCGGCTGCGGGCTGGGGCTAGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACAAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAGATAAATATGTTGTAACTAAATTGTTTAATATTATTGCGCGCTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATAtt                                                                                                                                                                                             <  2:204660/300‑1 (MQ=255)                                                                          cggcggCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATACCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGc                                                                                                                                                                                          <  2:603554/300‑1 (MQ=255)                                                                                     ggctggggctggCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTg                                                                                                                                                                               >  1:310831/1‑300 (MQ=255)                                                                                             ctggCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAacac                                                                                                                                                                       <  2:592291/300‑1 (MQ=255)                                                                                                  aaTTGTCCACTCTATAACACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGTGAACTGGTTGGTGCCCGCTTCTCGTTTCGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCt                                                                                                                                                                  <  2:606166/300‑1 (MQ=255)                                                                                                           ctctATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATNTTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGc                                                                                                                                                         >  1:481896/1‑300 (MQ=255)                                                                                                           ctctATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGc                                                                                                                                                         >  2:628572/1‑300 (MQ=255)                                                                                                           ctctATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAAAACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGc                                                                                                                                                         >  2:688912/1‑300 (MQ=255)                                                                                                             ctATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGccc                                                                                                                                                       >  1:93678/1‑300 (MQ=255)                                                                                                             ctATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGAATAAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGCGCAACAAAAGCTATTGGTTGccc                                                                                                                                                       >  2:49579/1‑300 (MQ=255)                                                                                                             ctATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTAGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACAAAAGCCATTGGTTGccc                                                                                                                                                       >  2:3878/1‑300 (MQ=255)                                                                                                                    aCTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGtt                                                                                                                                                >  1:578249/1‑300 (MQ=255)                                                                                                                       ggCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTtctc                                                                                                                                             >  1:130588/1‑300 (MQ=255)                                                                                                                             gggCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGcc                                                                                                                                       >  1:47823/1‑300 (MQ=255)                                                                                                                                                         gAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCAGGAGGTAAAAAAAGAGGATGAGCATAAt                                                                                                           >  1:261090/1‑300 (MQ=255)                                                                                                                                                         gAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATGTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAAt                                                                                                           >  2:419057/1‑300 (MQ=255)                                                                                                                                                          aacggggTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATc                                                                                                          <  1:187022/296‑1 (MQ=255)                                                                                                                                                           aCTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATcc                                                                                                         <  2:412829/300‑1 (MQ=255)                                                                                                                                                              gggTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTAACTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGNTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCt                                                                                                      >  2:524362/1‑300 (MQ=255)                                                                                                                                                                            tctcGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAgg                                                                                        <  1:673110/300‑1 (MQ=255)                                                                                                                                                                               cGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGAATCAACACTACCTTTCGCCAGGTGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGCATTGAGGTaa                                                                                     >  2:108508/1‑300 (MQ=255)                                                                                                                                                                                  ttAGCTGGCCGTTATGGCATAACATCCCGCAATGTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGGTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTGGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAaac                                                                                  <  2:543959/300‑1 (MQ=255)                                                                                                                                                                                       tGGCCGTTAGGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGTTAAAACAACAt                                                                             <  2:241796/300‑1 (MQ=255)                                                                                                                                                                                                  gCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCNTAGACCGACTCAACACTACCTTTCGCNAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAacga                                                                  >  2:474972/1‑300 (MQ=255)                                                                                                                                                                                                  gCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAacga                                                                  >  1:691989/1‑300 (MQ=255)                                                                                                                                                                                                  gCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTAGGCAacga                                                                  >  1:271496/1‑300 (MQ=255)                                                                                                                                                                                                   gcgagatgtgtataagagacagctGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAacgac                                                                 >  1:385352/23‑300 (MQ=255)                                                                                                                                                                                                       aCATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCANCACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACgcgc                                                             >  1:464830/1‑300 (MQ=255)                                                                                                                                                                                                                 atctacctccaccTGACACTACACGCACGATGTTCAAGTCACCACGACTATGCTATAAAAGAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGc                                                   <  1:293605/289‑1 (MQ=255)                                                                                                                                                                                                                         ctGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTc                                           >  1:406427/1‑300 (MQ=255)                                                                                                                                                                                                                           gCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGAGTCAt                                         >  2:544190/1‑300 (MQ=255)                                                                                                                                                                                                                           gCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCAGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCAt                                         >  2:544566/1‑300 (MQ=255)                                                                                                                                                                                                                                  acTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCTACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGTAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGcc                                  <  2:640455/300‑1 (MQ=255)                                                                                                                                                                                                                                            cGATGGTCAAGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATTCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGCCGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATttatttat                        >  2:231438/1‑300 (MQ=255)                                                                                                                                                                                                                                              aTGGTCAAGTCACCACGACTGTGCTATAAAANAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTNGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTAGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTc                      >  2:491713/1‑300 (MQ=255)                                                                                                                                                                                                                                                   aaaGTAACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTcaacaa                 <  1:708996/299‑1 (MQ=255)                                                                                                                                                                                                                                                    aaGTCACCACGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCaacaac                <  1:645942/300‑1 (MQ=255)                                                                                                                                                                                                                                                    aaGTCACCACGACTGTGCGAGAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCGGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGTAGGGAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCaacaac                <  2:224981/300‑1 (MQ=255)                                                                                                                                                                                                                                                        caccccGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCACCACTCCCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACAAAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCAGGAGGTAAAAAAGGGGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTAGGCAACGACGCGCAGTCGCTGGCGTTGCGTCCTTTCCGCCATTTATTTCCTCAACAACAGTc            >  1:552801/1‑300 (MQ=255)                                                                                                                                                                                                                                                        caccacGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTc            >  1:697688/1‑300 (MQ=255)                                                                                                                                                                                                                                                        caccacGACTGTGCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTc            >  2:677614/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gtgCTATAAAATAAATATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCNTAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCgc  >  1:494234/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                              GGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGC  >  NC_000913/1683960‑1684516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑