Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,549,651	G→A	A751T (GCT→ACT)	fdnG →	formate dehydrogenase N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,549,651	0	G	A	100.0%	162.0 / NA	47	A751T (GCT→ACT)	fdnG	formate dehydrogenase N subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/16);  total (31/16)

GGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCC  >  NC_000913/1549363‑1549939                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                 ggTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGAt                                                                                                                                                                                                                                                                                       >  1:600462/1‑300 (MQ=255)  gTATCTACCATCATCTGCGCGAGTTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTATATGACGCTAATGGCGTGCTTATTGCGAAGAAGGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAAACAGATGACTAACGGCGATa                                                                                                                                                                                                                                                                                      >  2:144919/1‑300 (MQ=255)                    gcgAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGGAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTc                                                                                                                                                                                                                                                                    <  2:90561/300‑1 (MQ=255)                       aGCTGTACCAGTCCAAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTgg                                                                                                                                                                                                                                                                 >  1:622314/1‑300 (MQ=255)                         cTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGAGCTGGAAGATCTATATGACGCTAATGGCGTGCTTACTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCCTGCTGGATCTACACCGGTAGCTGGACAGAGTAGGGCAACCAGATGACTAACCGCGATAACTCCTAACCGTCCGGTCTgggg                                                                                                                                                                                                                                                               >  2:266609/1‑300 (MQ=255)                           gTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGNGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGaa                                                                                                                                                                                                                                                             >  1:486098/1‑300 (MQ=255)                           gTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGaa                                                                                                                                                                                                                                                             >  1:561083/1‑300 (MQ=255)                                 gTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCt                                                                                                                                                                                                                                                       >  1:337314/1‑300 (MQ=255)                                 gTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTAAGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCt                                                                                                                                                                                                                                                       >  2:350550/1‑300 (MQ=255)                                                aGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGc                                                                                                                                                                                                                                        <  2:375702/300‑1 (MQ=255)                                                aGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGCGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCNAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGc                                                                                                                                                                                                                                        <  2:149343/300‑1 (MQ=255)                                                               gatgaAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCg                                                                                                                                                                                                                         >  1:545592/1‑300 (MQ=255)                                                                    aGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTANAGAGAACAACGGCTACGCGCTGGAAGAGCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTcgcg                                                                                                                                                                                                                    <  2:513055/300‑1 (MQ=255)                                                                        gAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCt                                                                                                                                                                                                                >  1:1815/1‑300 (MQ=255)                                                                            tGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCTACCCGTCCGGTCTGGGGAATACGCTTGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTAc                                                                                                                                                                                                            <  2:38103/300‑1 (MQ=255)                                                                              gAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACaa                                                                                                                                                                                                          >  1:321552/1‑300 (MQ=255)                                                                               aaCTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGCACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCANTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTNCACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAAc                                                                                                                                                                                                         <  2:563839/300‑1 (MQ=255)                                                                               aaCTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAAc                                                                                                                                                                                                         <  1:51982/300‑1 (MQ=255)                                                                                                                     ggCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATcc                                                                                                                                                                   >  1:366228/1‑300 (MQ=255)                                                                                                                              gaACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGAt                                                                                                                                                          >  1:606797/1‑300 (MQ=255)                                                                                                                                           gcgcTGTAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTgg                                                                                                                                             <  1:423717/300‑1 (MQ=255)                                                                                                                                            cgcTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGa                                                                                                                                            >  1:55925/1‑300 (MQ=255)                                                                                                                                                    gATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGAACCGTCCGGTCTGGGGAATACGCTGGGATGGGCATGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTAGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGgcagc                                                                                                                                    >  2:271942/1‑300 (MQ=255)                                                                                                                                                      tctcTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCaa                                                                                                                                  >  2:452151/1‑300 (MQ=255)                                                                                                                                                         ctATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTAGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTg                                                                                                                               >  1:516770/1‑300 (MQ=255)                                                                                                                                                                     tGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGa                                                                                                                   >  1:430248/1‑300 (MQ=255)                                                                                                                                                                      tgCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTGGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGat                                                                                                                  <  1:265510/299‑1 (MQ=255)                                                                                                                                                                      ggCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCGGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGat                                                                                                                  <  1:113281/300‑1 (MQ=255)                                                                                                                                                                        cGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACAACGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGAACCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTAGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGAAGGGTAACGatat                                                                                                                >  2:681022/1‑300 (MQ=255)                                                                                                                                                                                ttGCTAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACt                                                                                                        <  2:82640/300‑1 (MQ=255)                                                                                                                                                                                     aagaaAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGTACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGc                                                                                                   <  1:78306/300‑1 (MQ=255)                                                                                                                                                                                               aGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCAc                                                                                         <  1:644386/300‑1 (MQ=255)                                                                                                                                                                                                         tAGCTTNGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTANACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGcc                                                                               <  2:572216/300‑1 (MQ=255)                                                                                                                                                                                                                                               cttcttGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGAc                                         <  1:346843/300‑1 (MQ=255)                                                                                                                                                                                                                                                ttcttGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATCACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGNGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGCCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACg                                        <  2:610481/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTAGGCGGATATCAACGGTAAACCGTGGGATCNGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGCCTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCAGGAAGGGATGGGACGCCTGTTTGc                               >  2:161276/1‑300 (MQ=255)                                                                                                                                                                                                                                                          aTCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCCTGTTTGcc                              >  1:26267/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gTAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGNCGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCCTGTTTGCCATCAACaaaa                    >  2:608942/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     tAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCCTGTTTGCCATCAACAAAAt                   >  2:83528/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     tAGCTGGACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGCGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCCTGTTTGCCATCAACAAAAt                   >  1:107356/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          ggACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCAGGAAGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCgg              >  1:83545/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          ggACAGAGCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTAGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGCTGGGACGCATGTTTGCAATCAACAAAATGGCgg              >  2:338039/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               gagCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCATTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGt         >  1:711865/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               gagCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGt         >  1:208159/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               gagCAGGGCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCAAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGt         >  2:14320/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               gagCAGGGCAACCAGATGACTAACCGCGATAACTCCGAACCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCATGTTTGCAATCAAAAAAATGGCGGAAGGt         >  2:229690/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gCAACCAGATGACTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTcc  >  1:107060/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                 GGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTACGCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTGAGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCC  >  NC_000913/1549363‑1549939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑