Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,542,535	G→A	A19V (GCC→GTC)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,542,535	0	G	A	100.0%	200.4 / NA	60	A19V (GCC→GTC)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/22);  total (38/22)

ATTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGGCAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCCGC  >  NC_000913/1542260‑1542820                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                              aTTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCAACTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGa                                                                                                                                                                                                                                                                       >  1:357020/1‑300 (MQ=255)   ttCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTCTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAg                                                                                                                                                                                                                                                                     >  2:410670/1‑300 (MQ=255)          ggTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTCCGCTCTTTTGTTTGAAGTAGCGaa                                                                                                                                                                                                                                                              >  1:575044/1‑300 (MQ=255)          ggTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTAGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGaa                                                                                                                                                                                                                                                              >  1:142722/1‑300 (MQ=255)             tAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGc                                                                                                                                                                                                                                                           <  1:262691/300‑1 (MQ=255)               gcgcTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGACAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGcaa                                                                                                                                                                                                                                                         >  2:67025/1‑298 (MQ=255)                  cTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATcc                                                                                                                                                                                                                                                      >  1:284458/1‑300 (MQ=255)                    gTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCaa                                                                                                                                                                                                                                                    >  1:230958/1‑300 (MQ=255)                                ggAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTcat                                                                                                                                                                                                                                        >  1:210295/1‑300 (MQ=255)                                ggAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCAACAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGANTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTcat                                                                                                                                                                                                                                        >  2:171438/1‑300 (MQ=255)                                        ttGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGTTCAGGGCGAGTGCGCGGGTAGGCGGTCTGTTGGATTTCCCAGTTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGTTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGTATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCatgacatgac                                                                                                                                                                                                                                <  2:359561/300‑1 (MQ=255)                                        ttGCGCCACGCGGGCAGCCGCGAGGGTCATGATTGGGCAGGTAAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACTTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTTTCGAACTGCCANCGCTGTCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGNAAGCGATCCAAAAGTTTACTCatgatatgac                                                                                                                                                                                                                                <  2:494094/300‑1 (MQ=255)                                          gcgcCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGGCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTc                                                                                                                                                                                                                              <  1:259919/300‑1 (MQ=255)                                                                gTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGa                                                                                                                                                                                                        >  2:144059/1‑300 (MQ=255)                                                                     ggATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGa                                                                                                                                                                                                   <  1:644689/299‑1 (MQ=255)                                                                        ttGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGc                                                                                                                                                                                                <  1:273228/300‑1 (MQ=255)                                                                         tGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGcc                                                                                                                                                                                               <  1:75400/300‑1 (MQ=255)                                                                         tGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGcc                                                                                                                                                                                               <  2:303840/300‑1 (MQ=255)                                                                               ggTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCg                                                                                                                                                                                         >  1:258875/1‑300 (MQ=255)                                                                               ggTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCg                                                                                                                                                                                         >  2:630709/1‑300 (MQ=255)                                                                                        gAGTGCACTGGTAGTCGTTATGTTGGATTTCCCAGGTCACCAGACCATCTTTAACGTAGATGTGCCAGCTACAGGATCCTGTACAGTTAGCATCGTGGGTGGAACGCACGATGTTGTCGAACTGCCAACGCTGTCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCTACAAAGGCTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACACGATTCCTGCGCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGaca                                                                                                                                                                                <  2:209680/300‑1 (MQ=255)                                                                                             cgcgGGTAGTCGGTCTGTTGGATTTCCCAGATCACCAGACCATTTTTAACGTAGCTTTTCCAGTTACAGGAACCTGTATCGTTAACACCGTGGGTGGAACGCACGATTTTGCCGAACTGCCAACGCCGGTGATAGTTGTCCTAACAGTCGCGGTTGCTATGCATCCCCTGTCCGTGACTATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTAATGACATGACTAATGCTCCAAGAATGAATTGGGATCTGATGCATTGTCGGATGAGACACGGtt                                                                                                                                                                           >  2:63812/1‑300 (MQ=255)                                                                                                 ggTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTAc                                                                                                                                                                       <  2:690373/300‑1 (MQ=255)                                                                                                 ggTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATGTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTAc                                                                                                                                                                       <  1:326126/300‑1 (MQ=255)                                                                                                     gTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCa                                                                                                                                                                   >  1:590662/1‑300 (MQ=255)                                                                                                        ggTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGCTTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCGTCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCNGGAATGAATTGGGGTCTGATGCCTTGTCGGATGCGACACGGTTGTACNTCAGGc                                                                                                                                                                >  2:585929/1‑300 (MQ=255)                                                                                                           cTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCAACTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATCGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATc                                                                                                                                                             >  2:221537/1‑300 (MQ=255)                                                                                                             gTTGGATTTCCCAGGTCACCATACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGa                                                                                                                                                           <  2:106216/300‑1 (MQ=255)                                                                                                                                       tttttAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTccgc                                                                                                                                 <  1:119794/300‑1 (MQ=255)                                                                                                                                                  gATTTTGCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCTTGACCATCGACAAAGGTTTCGCCCTTTCGTTTGAATTAGCGAAAGCGATCCAAANGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTNATTTTTGGCTGAACTTCCGCCGACCATAAAc                                                                                                                      <  2:483767/300‑1 (MQ=255)                                                                                                                                                          aGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAgg                                                                                                              >  1:219627/1‑300 (MQ=255)                                                                                                                                                             tACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACGTCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTca                                                                                                           <  1:145391/300‑1 (MQ=255)                                                                                                                                                                 ggAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTcagcag                                                                                                       <  1:258884/300‑1 (MQ=255)                                                                                                                                                                   aGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTATACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCCTAAACCAGCCAGGTcagcagta                                                                                                     >  2:180297/1‑298 (MQ=255)                                                                                                                                                                     ccTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGNCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATNTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGcaca                                                                                                   >  2:661430/1‑300 (MQ=255)                                                                                                                                                                        gTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGNATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACaa                                                                                                >  2:625847/1‑300 (MQ=255)                                                                                                                                                                        gTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACaa                                                                                                >  1:174820/1‑300 (MQ=255)                                                                                                                                                                        gTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCAAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACaa                                                                                                >  2:65278/1‑300 (MQ=255)                                                                                                                                                                        gTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTACCAGTCGCGGTTGCTATGCATCAACTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCCTGACNTGACTCCTGCTCAAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGAAACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAAACAGCCAGGTCGACAGCCCACaa                                                                                                >  2:160323/1‑300 (MQ=255)                                                                                                                                                                          aCAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAAc                                                                                              >  1:191332/1‑300 (MQ=255)                                                                                                                                                                           cAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACg                                                                                             >  1:324512/1‑300 (MQ=255)                                                                                                                                                                            aGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGa                                                                                            >  1:198944/1‑300 (MQ=255)                                                                                                                                                                            aGTTAACACCGTGGGGGGAATGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCATTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGa                                                                                            <  2:300447/300‑1 (MQ=255)                                                                                                                                                                             gTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGac                                                                                           >  2:252066/1‑299 (MQ=255)                                                                                                                                                                             gTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGAt                                                                                           >  1:279021/1‑300 (MQ=255)                                                                                                                                                                             gTTAACACCGTGGGTGGAACGCACGATTTTGNCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTNTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGAt                                                                                           >  2:501262/1‑300 (MQ=255)                                                                                                                                                                                  cacCGTGGTTGGAACGCACGATTTTTTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCCGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTaga                                                                                      <  2:136676/300‑1 (MQ=255)                                                                                                                                                                                            ggAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCGGCAGCACACAAACGATGTAGAAGATTaaaaa                                                                            >  2:97774/1‑300 (MQ=255)                                                                                                                                                                                                               aaCTGCCAACGCTGGCGATAGCTGTCCTCCCATTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGAcc                                                         <  1:278119/300‑1 (MQ=255)                                                                                                                                                                                                                      aacactttcgagagccgTCCTCCCATTCGCGTTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTGGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGc                                                  <  2:8319/284‑1 (MQ=255)                                                                                                                                                                                                                      aaCGCTGGCGATAGCTGTCCTCCCAGGCGTGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGACTAAAAACACTTTCATCGCGCCGACCGGAGAGc                                                  <  2:120857/300‑1 (MQ=255)                                                                                                                                                                                                                                aTAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCCTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTNATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATAtt                                        >  1:471547/1‑300 (MQ=255)                                                                                                                                                                                                                                 tAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTg                                       <  1:571335/300‑1 (MQ=255)                                                                                                                                                                                                                                   gCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAg                                     >  1:444100/1‑300 (MQ=255)                                                                                                                                                                                                                                        cctccCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACa                                >  1:647298/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gCTATGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACCCAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCgg                >  2:221787/1‑300 (MQ=255)                                                                                                                                                                                                                                                           aTGCATCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGATGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCAGGTCATATTGAGCGACATGCCAAACGCCTGCGGAAt             >  1:36644/1‑300 (MQ=255)                                                                                                                                                                                                                                                                tCACCTGTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGTCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATaaaaa        >  1:44619/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCANGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAgccgc  >  1:476331/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gTCCGTGACCATCGACAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAgccgc  >  1:48490/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                              ATTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCAGCTACAGGAGCCTGTACAGTTAACACCGTGGGTGGAACGCACGATTTTGTCGAACTGCCAACGCTGGCGATAGCTGTCCTCCCAGTCGCGGTTGCTATGCATCACCTGTCCGTGACCATCGGCAAAGGTTTCGCCCTTTTGTTTGAAGTAGCGAAAGCGATCCAAAAGTTTACTCATGACATGACTCCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCCGC  >  NC_000913/1542260‑1542820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑