Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,276,230	C→T	P463P (CCG→CCA)	narX ←	sensor histidine kinase NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,276,230	0	C	T	100.0%	201.0 / NA	59	P463P (CCG→CCA)	narX	sensor histidine kinase NarX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/34);  total (25/34)

GCACGATCGCGCATTATTATCATGCCGTAGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACTGTTGCTGACGTTCCTGATGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTC  >  NC_000913/1275940‑1276520                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                   gcACGATCGCGCATTATTATCATGCCGTAGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGNCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAAt                                                                                                                                                                                                                                                                                           <  1:505115/300‑1 (MQ=255) gcACGATCGCGCATTATTATCATGCCGTAGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTTGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAAt                                                                                                                                                                                                                                                                                           <  2:411994/300‑1 (MQ=255)      aTCGCGCATTATTATCATGCCGTAGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAgg                                                                                                                                                                                                                                                                                      >  1:655237/1‑300 (MQ=255)        cgcgcgTTATTATCATGCCGTAGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCt                                                                                                                                                                                                                                                                                    <  2:416763/300‑1 (MQ=255)              ttattaTCATGCCGTAGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGTGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGa                                                                                                                                                                                                                                                                              <  2:542460/300‑1 (MQ=255)              ttattaTCATGCCGTAGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGa                                                                                                                                                                                                                                                                              >  2:261740/1‑300 (MQ=255)                      aTGCCGTAGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAg                                                                                                                                                                                                                                                                      >  1:544830/1‑300 (MQ=255)                        gCCGTAGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCAACGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCCCGGTGAGCTGCaaacg                                                                                                                                                                                                                                                                    >  2:543502/1‑297 (MQ=255)                           gTAGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGNCAGTTTGACCTGATTCTCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAACGTTTGAGGGCGTTACTTAATGCCTCCCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTNGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGaa                                                                                                                                                                                                                                                                 >  2:653056/1‑300 (MQ=255)                             aGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAAtg                                                                                                                                                                                                                                                               >  2:535298/1‑300 (MQ=255)                                     ctgcGGATGGCATTTTCATGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGc                                                                                                                                                                                                                                                       <  1:635203/300‑1 (MQ=255)                                                           cgccgcAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAgg                                                                                                                                                                                                                                 >  1:91378/1‑300 (MQ=255)                                                             ccgcAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGAt                                                                                                                                                                                                                               <  1:112456/300‑1 (MQ=255)                                                                  gCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCAtt                                                                                                                                                                                                                          >  1:384482/1‑300 (MQ=255)                                                                   ccGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTc                                                                                                                                                                                                                         <  1:674100/300‑1 (MQ=255)                                                                   ccGTTATCCTGGACGGTCAATTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTAACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTAACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCACAGGATGCATTc                                                                                                                                                                                                                         >  2:42256/1‑300 (MQ=255)                                                                        aTCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTc                                                                                                                                                                                                                    >  1:372428/1‑300 (MQ=255)                                                                        aTCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTAGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCGGTTc                                                                                                                                                                                                                    <  1:583326/300‑1 (MQ=255)                                                                        aTCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTAACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGACTGTGGTGAGCAATTCACGCAAATGCGCACAGGATGCATTAAGTTc                                                                                                                                                                                                                    >  2:178527/1‑300 (MQ=255)                                                                        aTCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCAACACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCAACGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGTGGAATGTGGTGAGCAATTCACGCAACTGCGCACAGGATGTATTAAGTTc                                                                                                                                                                                                                    >  2:102496/1‑300 (MQ=255)                                                                                      gTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTACTGCCTAACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTAACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCCTTCAGTTCGTTACGGATCTGAc                                                                                                                                                                                                      >  1:673624/1‑300 (MQ=255)                                                                                           aCCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAAc                                                                                                                                                                                                 <  1:233064/300‑1 (MQ=255)                                                                                             cTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCTGGAAGCCAAATTTGGCGCTGTACNCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATNCATTCAGTTCGTTACGGATCTGACTTAACAg                                                                                                                                                                                               <  2:503308/300‑1 (MQ=255)                                                                                             cTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAg                                                                                                                                                                                               <  1:315521/300‑1 (MQ=255)                                                                                             cTGATTATCGTTTTGCGCCACCGGCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAg                                                                                                                                                                                               <  1:440223/300‑1 (MQ=255)                                                                                               gATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGtt                                                                                                                                                                                             >  1:674850/1‑300 (MQ=255)                                                                                                                   gTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCATCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGTGGAATGTGGTGAGCAATTCACGCAACTGCGCACAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAgc                                                                                                                                                                         >  2:343834/1‑300 (MQ=255)                                                                                                                    tCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAAcctgtctcttatacacatctcgcagg                                                                                                                                                                        <  1:703082/300‑26 (MQ=255)                                                                                                                                 aCTCGCNTTCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAANAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCAt                                                                                                                                                           <  2:574798/300‑1 (MQ=255)                                                                                                                                  cTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACNAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGNTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATc                                                                                                                                                          <  2:653333/300‑1 (MQ=255)                                                                                                                                         gCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAaac                                                                                                                                                   <  1:355197/300‑1 (MQ=255)                                                                                                                                         gCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAaac                                                                                                                                                   <  2:402304/300‑1 (MQ=255)                                                                                                                                         gCGAATGTTTGAGGGCGTTACTTAATACCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCTGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAaac                                                                                                                                                   <  2:195929/300‑1 (MQ=255)                                                                                                                                               gTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCa                                                                                                                                             >  1:622064/1‑300 (MQ=255)                                                                                                                                                tttGAGGGCGCTACTTAATGCCTAACGGGCAACTTGCAACAAGTGGATTGCCTGACGCGAAGACACCAGGCGAGGCGGCAATTGATAATCCAGCTTCAACGGGAAGCCAAATTTGGCGCTGTACTCTCCGCACCTCGCCTCCAGCGCTGGACGTAATCCAGGCTAGGTGAGCTGCCAGCGGCCTGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTTAGTTCATTACGGCTCTGACTTCACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAAATCAc                                                                                                                                            >  2:238464/1‑300 (MQ=255)                                                                                                                                                 tttaGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAANTTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCAcc                                                                                                                                           <  1:487965/297‑1 (MQ=255)                                                                                                                                                       gCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGCCGTCATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGACCTGCATCTGTAAACAACTCACCTGCATc                                                                                                                                     >  2:317059/1‑300 (MQ=255)                                                                                                                                                       gCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATc                                                                                                                                     >  1:186750/1‑300 (MQ=255)                                                                                                                                                            aCTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAAAAGTTCGCGGCTGCTTTCTGGCAGCGCATCGACCTGCATCTGTAAACAACTCACATGCATCTTCAt                                                                                                                                >  2:366363/1‑300 (MQ=255)                                                                                                                                                            aCTTAATGCCTAACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTNCGCCCAGGATGCATTCAGTTCGTTACGGATCTGAATTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAACCAACTCACCTGCATCTTCAt                                                                                                                                >  2:624958/1‑300 (MQ=255)                                                                                                                                                              ttAATGCCTCATTTGCAATTTGCAACGAGTGGATTGCCTGATGCGAAGACACCATGCGAGGCGGCAATGGATAATCCAGCTTCACTGGTAAGCCAGATTTGGCACTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGTTGAGCGACAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCATGATGCATTTAGTTCGTTACAGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACCCACCTGCATCTTCATGc                                                                                                                              <  2:8084/300‑1 (MQ=255)                                                                                                                                                               tAATGCCTCACGGGCAATTTGCAACAAGGGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCa                                                                                                                             <  1:570803/300‑1 (MQ=255)                                                                                                                                                                         cGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACGCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGAt                                                                                                                   <  1:110659/300‑1 (MQ=255)                                                                                                                                                                                             gATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACNGGGAAGCCAAATTTGGCGCTGTACTCTCCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAg                                                                                               <  2:507910/300‑1 (MQ=255)                                                                                                                                                                                                 gCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCATGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTcg                                                                                           <  1:27495/300‑1 (MQ=255)                                                                                                                                                                                                     gATGCGAATGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCTGGAAGCNAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACNCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTcgcgcg                                                                                       <  2:661221/300‑1 (MQ=255)                                                                                                                                                                                                              gCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGTGCAATAGAATCATGCAGTTCGCGCGCAAtggtgg                                                                              <  2:305638/300‑1 (MQ=255)                                                                                                                                                                                                              gCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGTGCAATAGAATCATGCAGTTCGCGCGCAAtggtgg                                                                              >  1:307926/1‑300 (MQ=255)                                                                                                                                                                                                                aCCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCTGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCa                                                                            <  2:418129/300‑1 (MQ=255)                                                                                                                                                                                                                aCCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCa                                                                            <  2:540224/300‑1 (MQ=255)                                                                                                                                                                                                                        agttggcAATTGATAATACAGCTTCATCGGGAAGCCAAACTGGGCACTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCACGCTGAATGTGGTGAGCAATTCACGCAACTGCGCCTAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGGTCGCGGCTGCTTTCTGGCGGCGCATCGCCATGCATCTGTAAACAACTCAACTGCGTTTTCATGCACGAGATAGATTGGGCAATAGGATCATGCAGTTCGCGCGCAAGGGTGGCACGcgcggg                                                                    <  2:201016/296‑6 (MQ=255)                                                                                                                                                                                                                           cggcAATTGATAATCCAGCTTCACCGGGAAGCCAAGTTTGGCGCTGTACTCTTCNCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGANAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCGCTTCCAt                                                                 <  2:661130/300‑1 (MQ=255)                                                                                                                                                                                                                           cggcAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCAt                                                                 <  1:276297/300‑1 (MQ=255)                                                                                                                                                                                                                                     tAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTACAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTACATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTTTTCCATCACGATCAAc                                                       >  2:69380/1‑300 (MQ=255)                                                                                                                                                                                                                                      aaTCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACt                                                      >  1:342359/1‑300 (MQ=255)                                                                                                                                                                                                                                                           cAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACTGTTGCTGACGTTCCTGATGGc                                 <  1:320413/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        gTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCNTGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACTGTTGCTGACGTTCCTGATGGCGATccagcgccag                    >  1:485719/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          aCTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGACCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGNTCTGACTTAACAGTTCGCTGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTNACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACTGTTGCTGACGTTCCTGATGGCGATccagcgccagcg                  <  2:562868/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          cTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGATAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACTGTTGCTGACGTTCCTGATGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTc  <  2:335324/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                   GCACGATCGCGCATTATTATCATGCCGTAGTGATTGCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACTGTTGCTGACGTTCCTGATGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTC  >  NC_000913/1275940‑1276520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑