Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,265,552	G→A	G181S (GGT→AGT)	prfA →	peptide chain release factor RF1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,265,552	0	G	A	100.0%	195.8 / NA	57	G181S (GGT→AGT)	prfA	peptide chain release factor RF1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/30);  total (27/30)

AAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGC  >  NC_000913/1265262‑1265838                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                               aaGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATcgcg                                                                                                                                                                                                                                                                                       >  1:281078/1‑300 (MQ=255)         gAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACgt                                                                                                                                                                                                                                                                               <  1:260236/300‑1 (MQ=255)                gCGAGCAACTGGAACAGCAATTACAGTGTCTGTTACTGCCAAGAGATTCGGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGTTGTTCGCGGGCAATCTGTTCCGTATGTACAGCCGTTACGCCTAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCGGCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGGTccgg                                                                                                                                                                                                                                                                        <  2:345182/300‑3 (MQ=255)                       aCTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGa                                                                                                                                                                                                                                                                 >  1:23727/1‑300 (MQ=255)                              cAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAg                                                                                                                                                                                                                                                          <  1:369603/300‑1 (MQ=255)                                gCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGACATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAggg                                                                                                                                                                                                                                                        >  2:675153/1‑300 (MQ=255)                                        cggTTCTGCTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGTTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCTGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTc                                                                                                                                                                                                                                                <  2:93226/299‑1 (MQ=255)                                             cTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTTTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGANCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACt                                                                                                                                                                                                                                           >  1:511232/1‑300 (MQ=255)                                                     gCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTg                                                                                                                                                                                                                                   >  1:359505/1‑300 (MQ=255)                                                        aaaaGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAATAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTAc                                                                                                                                                                                                                                <  1:225329/300‑1 (MQ=255)                                                         aaaGCTCCTGATGACGAACGTAACGTCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCTACGAAGCGGCGCTGTTCGTGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTANAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTAcc                                                                                                                                                                                                                               <  2:163693/300‑1 (MQ=255)                                                         aaaGATCCTGATGACGAACTTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCTGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTANAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTAcc                                                                                                                                                                                                                               <  2:164419/300‑1 (MQ=255)                                                                     gaCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATg                                                                                                                                                                                                                   >  1:76502/1‑300 (MQ=255)                                                                          aCGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGa                                                                                                                                                                                                              >  2:312680/1‑300 (MQ=255)                                                                          aCGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCAAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGa                                                                                                                                                                                                              >  1:70145/1‑300 (MQ=255)                                                                            ttAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAAc                                                                                                                                                                                                            <  2:675319/299‑1 (MQ=255)                                                                            gTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAAc                                                                                                                                                                                                            <  1:330505/300‑1 (MQ=255)                                                                                       cTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCa                                                                                                                                                                                                 >  1:260697/1‑300 (MQ=255)                                                                                       cTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCAAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCa                                                                                                                                                                                                 >  1:659282/1‑300 (MQ=255)                                                                                           aagttcgagtcggggccGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAATAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAAc                                                                                                                                                                                             <  2:337993/285‑1 (MQ=255)                                                                                           aaGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAAc                                                                                                                                                                                             <  1:31369/300‑1 (MQ=255)                                                                                                  aGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGa                                                                                                                                                                                      >  1:230894/1‑300 (MQ=255)                                                                                                       gAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCa                                                                                                                                                                                 >  2:671928/1‑300 (MQ=255)                                                                                                         aCCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAAc                                                                                                                                                                               <  1:595134/300‑1 (MQ=255)                                                                                                                        gcggcgCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGc                                                                                                                                                                <  1:655604/300‑1 (MQ=255)                                                                                                                          ggcgCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCAt                                                                                                                                                              <  1:637257/300‑1 (MQ=255)                                                                                                                                gTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCNTCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATAc                                                                                                                                                        <  1:479686/300‑1 (MQ=255)                                                                                                                                gTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATAc                                                                                                                                                        >  2:194186/1‑300 (MQ=255)                                                                                                                                gTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATAc                                                                                                                                                        <  1:25123/300‑1 (MQ=255)                                                                                                                                          gATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCg                                                                                                                                              >  1:278589/1‑300 (MQ=255)                                                                                                                                             cTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGNGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCa                                                                                                                                           >  2:153068/1‑300 (MQ=255)                                                                                                                                              tGTTCCGTATGGACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAg                                                                                                                                          <  1:351344/300‑1 (MQ=255)                                                                                                                                                        gTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGggtgg                                                                                                                                >  1:673076/1‑300 (MQ=255)                                                                                                                                                          aCAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGGACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGTTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTc                                                                                                                              <  2:197147/300‑1 (MQ=255)                                                                                                                                                              ccGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCa                                                                                                                          >  1:415660/1‑300 (MQ=255)                                                                                                                                                               cGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCAc                                                                                                                         <  1:568650/300‑1 (MQ=255)                                                                                                                                                                      ccGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCCGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAAAGTGTTCCTACCACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTAGTAAGGGGCGGGTGGTCAGCACGTTAaca                                                                                                                  >  2:283079/1‑300 (MQ=255)                                                                                                                                                                       cGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGTTAAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAacac                                                                                                                 <  2:232156/300‑1 (MQ=255)                                                                                                                                                                                                       gcgcCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTGCGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGc                                                                                 >  2:598809/1‑300 (MQ=255)                                                                                                                                                                                                                 gggTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTNCGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGAt                                                                       <  1:463637/300‑1 (MQ=255)                                                                                                                                                                                                                     gAACATGGTGGTTGTAAAGAGATCATCGCCAAAATTAGCGGTGATTGTGTGTATGGTCGTCGGAAATTTGAATCCTGCAGTCATCGCGGGCAACGTGTTCCTGCTGCGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATTAACCCAGCAGATTTACGCATTGATACTCTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGAttgtt                                                                   <  2:253129/300‑1 (MQ=255)                                                                                                                                                                                                                      aaCATGGGGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGAttgttg                                                                  <  1:144838/300‑1 (MQ=255)                                                                                                                                                                                                                      aaCATAGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGAttgttg                                                                  <  1:372013/300‑1 (MQ=255)                                                                                                                                                                                                                          tggtggTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGa                                                              >  1:694141/1‑300 (MQ=255)                                                                                                                                                                                                                           ggtggtTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGaa                                                             <  1:366561/300‑1 (MQ=255)                                                                                                                                                                                                                                   taAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGa                                                     <  1:633312/300‑1 (MQ=255)                                                                                                                                                                                                                                    aaaGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGAc                                                    >  1:352354/1‑300 (MQ=255)                                                                                                                                                                                                                                             ctgcgagatgtgtataagagacaggtgtATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGATCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTca                                           >  1:351153/25‑300 (MQ=255)                                                                                                                                                                                                                                                 ccAAAATTATCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGGCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCacaac                                       <  2:532498/300‑1 (MQ=255)                                                                                                                                                                                                                                                 ccAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCacaac                                       <  1:19439/300‑1 (MQ=255)                                                                                                                                                                                                                                                  caaaattaacattGATGGTGTGTGTGGTCGTCGGAAATTTGAATCCGTCAGTCATCGCGTGCAACGTGTTCCTGCTACTGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCacaaca                                      <  2:189171/288‑1 (MQ=255)                                                                                                                                                                                                                                                                  ggTGTGTATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTaaa                      >  2:146865/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gtgtATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGNATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCa                   >  2:522054/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gtgtATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAACAACAAAGCTAAAGCa                   >  2:134661/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gtgtATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCa                   >  1:43141/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gtgtATGGTCGTCTGAAATTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCNACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGNATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCa                   >  2:644378/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      aTTTGAATCCGGCAGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGc  <  1:434108/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                               AAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGC  >  NC_000913/1265262‑1265838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑