Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,159,4150CG29.7% 83.9 / 12.1 46L344V (CTG→GTG) mdtCmultidrug efflux pump RND permease subunit MdtC
Reads supporting (aligned to +/- strand):  ref base C (8/18);  new base G (11/0);  total (28/18)
Fisher's exact test for biased strand distribution p-value = 1.26e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGT  >  NC_000913/2159126‑2159713
                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                            
cTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑t                                                                                                                                                                                                                                                                                                   <  1:586607/300‑1 (MQ=255)
         cggcggCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTAGGTGG‑TGGTGGTGGTTC‑T‑AGTGGTGtt                                                                                                                                                                                                                                                                                         >  2:248779/1‑300 (MQ=255)
                 cgGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTcc                                                                                                                                                                                                                                                                                 <  1:337918/300‑1 (MQ=255)
                      cGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCNAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTAGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTgcgc                                                                                                                                                                                                                                                                            >  1:504702/1‑300 (MQ=255)
                                         cGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGATGGTGGTTC‑T‑GGTGGTGGTTTTATTCCTGCGCTCGGGTCGTGCCACtatta                                                                                                                                                                                                                                                         >  2:419916/1‑300 (MQ=255)
                                           gTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTattatt                                                                                                                                                                                                                                                       <  1:398911/300‑1 (MQ=255)
                                           gTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGATGGTGATTC‑T‑GGTGGTGTTTTTATTCATGCGCTTGGGTCGCGCCACTattatt                                                                                                                                                                                                                                                       >  2:131307/1‑300 (MQ=255)
                                             gACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGATGGTACTTT‑T‑GGGGGTGGGGTTGTTCCTGGGCTAGGGTCGCGCCACgattgttgc                                                                                                                                                                                                                                                     >  2:243791/1‑295 (MQ=255)
                                             gACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTAGGTGG‑TGATGGTGATTT‑T‑GGTGGTGTTTTTATTCCTgcgttggggtagcgaaaagattattca                                                                                                                                                                                                                                                     >  2:289087/1‑276 (MQ=255)
                                                       gTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGGTAGTGGTGC‑T‑AGTGGTGTTTTTATTCCTGCGCTCGGGTTGCGCAACTATTATTCCCGCGGTTTCg                                                                                                                                                                                                                                           >  2:143573/1‑300 (MQ=255)
                                                       gTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGATAGTGATTC‑T‑GGTGGTGTTTTTATTTCTGGGTTCGGGTCGCGCCAATATTATTCCCGCCGTTTCg                                                                                                                                                                                                                                           >  2:575645/1‑300 (MQ=255)
                                                       gTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑AGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCg                                                                                                                                                                                                                                           >  2:150681/1‑300 (MQ=255)
                                                       gTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTAGGTGG‑TGGTGGTGGTTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCg                                                                                                                                                                                                                                           >  2:459346/1‑300 (MQ=255)
                                                       gTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTAGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGATGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGtttag                                                                                                                                                                                                                                           >  2:298777/1‑298 (MQ=255)
                                                             gATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGNAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCNCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGGTGGTAATGC‑T‑GGGGGTGTGTTTCTTCATGCGGTCGGGTGGCGCCAATGTTATTCACGACGTTTAGGTGcag                                                                                                                                                                                                                                     >  2:599963/1‑298 (MQ=255)
                                                                     cAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCt                                                                                                                                                                                                                             <  1:641416/300‑1 (MQ=255)
                                                                                                 gCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCtg                                                                                                                                                                                                 <  1:496897/300‑1 (MQ=255)
                                                                                                              tgatCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGtc                                                                                                                                                                                    <  1:311810/300‑1 (MQ=255)
                                                                                                                 tCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCa                                                                                                                                                                                 >  1:636807/1‑300 (MQ=255)
                                                                                                                         cTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGATGGTGGTTT‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTACTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCtt                                                                                                                                                                         >  2:454642/1‑300 (MQ=255)
                                                                                                                           gCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGGTAGTGATTC‑T‑GGCGGTGTTTTTATTCCTGGGCTTGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGTTGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCACTAACCTTTc                                                                                                                                                                       >  2:310942/1‑300 (MQ=255)
                                                                                                                                 aGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTAGGTGG‑TGCTGGTGGTTT‑T‑GGTGGTGGTGTTATTCATGCGCTCGGGTAGCGCCGATATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGGTTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTATCAATAACCTTTCGTTAAt                                                                                                                                                                 >  2:292695/1‑300 (MQ=255)
                                                                                                                                      atatTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGANGTCGAGCAAACGCTGATTATCTCGGTGG‑TGGTGGTGATTC‑T‑GGTGGTGGTTTTATTCCTGCNCTCGGGTCGCGCCACTATTATTCCCGCAGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGgcgc                                                                                                                                                            >  2:597076/1‑300 (MQ=255)
                                                                                                                                                  cGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTAGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTGAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTa                                                                                                                                                >  2:640188/1‑300 (MQ=255)
                                                                                                                                                    gTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGGTGGTGGTTT‑T‑GGTGGGGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTAGGTGCAGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTaat                                                                                                                                              >  2:89527/1‑298 (MQ=255)
                                                                                                                                                    gTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGGTGGTGCTTC‑T‑GGTGGTGTTTTTATTNCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCGGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACt                                                                                                                                              >  2:186278/1‑300 (MQ=255)
                                                                                                                                                    gTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGGTGGTGGTGC‑T‑GGGGGTGGGGTTATTGATGGGCTGGGGTAGCGAAGATATTATTCACGCAGTGTCGGTGCCGGTTTCGCTGATTGGTATGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTaat                                                                                                                                              >  2:139416/1‑298 (MQ=255)
                                                                                                                                                                              ggAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCgt                                                                                                                    <  1:712948/300‑1 (MQ=255)
                                                                                                                                                                                                                         gCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CCACAGTGGCCC‑T‑AAGGGTGTTGTGGTTCATGCGCTCGGGTCGCGACACTATTATTCCCGCAGTTTCGGTGCCGGTTTCGATGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGaaa                                                                         >  2:285977/1‑300 (MQ=255)
                                                                                                                                                                                                                                  cGCTCCCCAACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GTTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCaa                                                                <  1:411309/300‑1 (MQ=255)
                                                                                                                                                                                                                                    cTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGc                                                              <  1:159988/300‑1 (MQ=255)
                                                                                                                                                                                                                                            ccaTTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CCCCAGTACCCC‑T‑GACGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGANTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGCTGCAAGCCGCACTGc                                                      >  2:182069/1‑300 (MQ=255)
                                                                                                                                                                                                                                                 cgctccTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGt                                                 <  1:731939/296‑1 (MQ=255)
                                                                                                                                                                                                                                                 cgcgCCTCGCGGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGTTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGAGTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGt                                                 <  2:397391/300‑1 (MQ=255)
                                                                                                                                                                                                                                                 agcgcCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGt                                                 <  1:653514/299‑1 (MQ=255)
                                                                                                                                                                                                                                                        cGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGGTGGTGGTTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATNGGTACGTTTGCGGCGATGTACCTGTGNGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTcgcg                                          >  2:500317/1‑300 (MQ=255)
                                                                                                                                                                                                                                                              aagaagTCGAGCAAACGCTGATTATCTCGGTGGTCGC‑AGT‑ATCC‑TTAACAGTGGTGTTATTCATGCGGTAGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCAATCGTGGTGCTGGAAAACATTGCACGTCATATGGAAGCGGGAATGAAACAGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCg                                    >  2:366274/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                   gTCGAGCAAACGCTGATTATCTCGGTGG‑TAGTAGT‑AGTC‑TTGGGGGTGGTTTTATTCATGCGCTCGGGTCGTGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTCATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGANGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGtttt                               >  2:553178/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                       aGCAAACGCTGATTATCTCGGTGGTCG‑TGGT‑ATCCCT‑AACGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATTGCTACTGGTTTCGTGGTGGATGACGCAATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACgg                           >  2:232666/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                             cGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGt                     >  1:425877/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                 gATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGAt                 <  1:187258/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  attgtctcGGTGG‑CGCTGTTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTTCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAGACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATg                <  1:254281/296‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    tATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAg              <  1:115597/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      tctcGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTc            <  1:57127/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                            tGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCTGTTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCAc      <  1:366927/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                            tGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCAc      <  1:617628/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                              g‑cgcTGGTGATTC‑T‑GGTGGTGTTCTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGCGTCTGTCACtg    >  1:79460/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                 gcTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACtggt  <  1:748544/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                            
CTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTC‑T‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGT  >  NC_000913/2159126‑2159713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑