Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	896,908	C→T	P259S (CCG→TCG)	potH →	putrescine ABC transporter membrane subunit PotH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	896,908	0	C	T	100.0%	196.1 / NA	59	P259S (CCG→TCG)	potH	putrescine ABC transporter membrane subunit PotH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/33);  total (26/33)

ACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGCGCCAATGCTGATGCTGGTTATCTATTCGTTTAACAGCTCGAAGC  >  NC_000913/896615‑897201                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      actttgtgCTGAATAATTTTCTGCTGTTGCTGGTGGTTATCGATCACCCGCTGACCATTCTGCATACCAATCTGGCCTTTTATATCTGCATTGTTTACTCTTACGTGCCGTTTATGGTATGGACGATTTATACTGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAAc                                                                                                                                                                                                                                                                                                 <  2:179168/296‑1 (MQ=255) acGGTGTGCTGAGTAATTTTTGGCTGTGGCTGGGGGGTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGGTTTATACCGCGTTGATTCGTATTGATTATTTGTTGGTGGGAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAAc                                                                                                                                                                                                                                                                                                 <  2:450120/300‑1 (MQ=255)      atgCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCCCGACCGCTGAAAACGTTCTTTACTGTGATCGTGCAGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGCACTGCTc                                                                                                                                                                                                                                                                                            >  2:251939/2‑300 (MQ=255)         cTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGt                                                                                                                                                                                                                                                                                         <  1:380969/300‑1 (MQ=255)           gAATAATTTTCTGCTGTGGCTGTGGGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTgg                                                                                                                                                                                                                                                                                       <  1:177139/300‑1 (MQ=255)           gAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTgg                                                                                                                                                                                                                                                                                       <  1:400681/300‑1 (MQ=255)           gAATAATTTTCTGCTGTGGCTGGGAGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCACTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTNCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGNGCGAGTTTGTGATCTCGGAACTGCTCGGTgg                                                                                                                                                                                                                                                                                       <  2:490912/300‑1 (MQ=255)                  tttCTGCGGTGGCTTGGGGTTATCGATCGACCGCTGACCATTTTGCATGCTAATCTGGCCGTTTACATCGGCATTGTTGACGCTTGCGTGCCGTTTATGGTACTGCCGATTTATACCGCGGTGAGTCGTATTGATTATTCGCTGGTGGAAGCAGCGTTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTATTGTGATCGTGCCGTTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCACGGag                                                                                                                                                                                                                                                                                <  2:361788/300‑2 (MQ=255)                  tgtataatagacaggggtgTTATCAATCAACCGCTGACCACTCTGCATCCCACCCCTTCCGTTTACATCGACAGTGTTTACGCTTACGGGCCGTTGAGGGTGCTGCCGATTGATACCGCGTTGATTCGTATTGATTATTCGTGGGGGGAAGCAGCGCTGGATTTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGag                                                                                                                                                                                                                                                                                <  2:57877/282‑2 (MQ=255)                            tgCTGCGGGTTTTCGATCAACCCCTGACCAGTCTGCATACCAGTCCCGCCTTTTATATCAGCATTGTTTACGCTTACGTGCCGTTTCTGGTACTGCCTATTTATACCGAGTTGATTCGTATTGATTACTCGCTGTTGGAAGCAACGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTGACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGCTTGTCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGa                                                                                                                                                                                                                                                                      <  2:372601/299‑1 (MQ=255)                            ggCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGa                                                                                                                                                                                                                                                                      >  1:571632/1‑300 (MQ=255)                                gggggTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGAcctgtctcttataca                                                                                                                                                                                                                                                                  >  1:58054/1‑286 (MQ=255)                                        tCGATCAACCGCTGACCATTCTGCATACCAATCTGTCCGTTTATATCTGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGc                                                                                                                                                                                                                                                          <  2:523475/300‑1 (MQ=255)                                           aTCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTAt                                                                                                                                                                                                                                                       >  1:582967/1‑300 (MQ=255)                                             cAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTTCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATgg                                                                                                                                                                                                                                                     <  2:425998/300‑1 (MQ=255)                                                            cTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACTGATGAAAACATTCTTTAATGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCAGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGAAAGAGTTTTTCAAt                                                                                                                                                                                                                                      >  2:81106/1‑300 (MQ=255)                                                                 tACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACcg                                                                                                                                                                                                                                 <  1:176981/300‑1 (MQ=255)                                                                                 tataTCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGcc                                                                                                                                                                                                                 <  1:163528/300‑1 (MQ=255)                                                                                      cGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTcggc                                                                                                                                                                                                            <  1:527041/300‑1 (MQ=255)                                                                                           ttGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAg                                                                                                                                                                                                       <  1:171660/300‑1 (MQ=255)                                                                                             gTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGTCCGCCCGCTGACCACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCg                                                                                                                                                                                                     >  2:261667/1‑300 (MQ=255)                                                                                             gTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCCCGACCGCTGACCACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCg                                                                                                                                                                                                     >  2:365713/1‑300 (MQ=255)                                                                                             gTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCg                                                                                                                                                                                                     >  2:393258/1‑300 (MQ=255)                                                                                                       aCGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGt                                                                                                                                                                                           <  1:400623/300‑1 (MQ=255)                                                                                                          ttccGTTGATGGTACTGCCGATTTATACCGCATTGATTCGTATTAATTATTCGCTGGTTGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTTTGATCGTGCCGCTGACGAAAGGTGGGATTATTGCCGGATCGGTGCTGGTGTTTATCTCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgc                                                                                                                                                                                        <  2:50964/298‑1 (MQ=255)                                                                                                          tGCCGTTTATGGGACTGCCGATTTATACCGCTTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgc                                                                                                                                                                                        <  2:614680/300‑1 (MQ=255)                                                                                                            ccGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCCCGACCGCTGAAAACGTTCTTTACTGTGATCGTGCAGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgctg                                                                                                                                                                                      >  2:331798/1‑300 (MQ=255)                                                                                                              gTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCCCGCCCGCTGACAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTcgctgct                                                                                                                                                                                    >  2:364711/1‑300 (MQ=255)                                                                                                              gTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCCCGCCCGCTGACAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTAGGCGGTAGCGATCATCATGTtgctgct                                                                                                                                                                                    >  2:216071/1‑300 (MQ=255)                                                                                                                    gttACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAAt                                                                                                                                                                              <  1:51497/298‑1 (MQ=255)                                                                                                                    ggTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAAt                                                                                                                                                                              <  1:51504/300‑1 (MQ=255)                                                                                                                      tgcTGCCGAGTTATGCTGCGTTGATTCGTGGTAATTATTCGCTGGTGGAAGCGGCGATGGATCGCGGGGCACTACCGCTGAAAACGTTCTTTACTTCGGTCGTGCCGTTGACTGAAGGTGGTATTATTGCCGGATCGATGCTGGTGTTTATCCCGGTTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATtg                                                                                                                                                                            <  1:285376/298‑1 (MQ=255)                                                                                                                             gATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGAt                                                                                                                                                                     >  1:579530/1‑300 (MQ=255)                                                                                                                                       gcgTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTc                                                                                                                                                           >  2:388438/1‑300 (MQ=255)                                                                                                                                            gATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACaa                                                                                                                                                      <  1:388429/300‑1 (MQ=255)                                                                                                                                                             cGCTGGTGGAATCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTANTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCANGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCg                                                                                                                                     <  2:640654/300‑1 (MQ=255)                                                                                                                                                                                     tcgttGCACGGCCGCTGGAAGCGTTCTTTACTGTGATCGTGCCGCTGACTAAATNTGGGATTATTGCCTGATCGATGCTGGTGTTTATCCCTGCTGTGGTCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGANAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGaataatgt                                                                                                             <  2:633461/296‑3 (MQ=255)                                                                                                                                                                                        gTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTAcc                                                                                                          >  2:629613/1‑300 (MQ=255)                                                                                                                                                                                               aCCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGTCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGNTATGGCAAGAGTTTTTCAATAACCGCNACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCTGTAgtt                                                                                                   <  2:472648/300‑1 (MQ=255)                                                                                                                                                                                               aCCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGNTATGGCAAGAGTTTTTCAATAACCGCNACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCTGTAggt                                                                                                   <  2:473244/300‑3 (MQ=255)                                                                                                                                                                                                           gTTCTTTACTGTGATCGTGCCGCTGACTAGAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGATGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGCAGTTCGTCCGCCCTgg                                                                                       >  2:89586/1‑300 (MQ=255)                                                                                                                                                                                                                  aCTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTCCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCACTGGCGGATtg                                                                                >  2:119184/1‑300 (MQ=255)                                                                                                                                                                                                                      tgATCGTGCAGCTGACTAAAGGTTGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGAt                                                                            <  1:513021/300‑1 (MQ=255)                                                                                                                                                                                                                         tCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGAtttt                                                                         >  2:658239/1‑300 (MQ=255)                                                                                                                                                                                                                                   gACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGc                                                               >  2:533254/1‑300 (MQ=255)                                                                                                                                                                                                                                   gACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGc                                                               >  1:541378/1‑300 (MQ=255)                                                                                                                                                                                                                                         aGGTGGGGTAATTGCCGGGTAGAGGCTGGTGTTTATCCCGGCGGTGGGCGAGTTTGTGACCTCGGAACGGCTCGTTGGCCCGGACAGCATAATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAGAACCGCGACTGGCCGGTGGCCTCGGCGGGAGCGATCATCATGTTGCTGCGGCTAATTGTGCCGAGAAGGTGGTTCCAAAAACACCAGAAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTAcc                                                         <  1:382623/300‑1 (MQ=255)                                                                                                                                                                                                                                               gATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTtctc                                                   <  1:110801/300‑1 (MQ=255)                                                                                                                                                                                                                                                       ccGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGCGcc                                           <  2:440744/300‑1 (MQ=255)                                                                                                                                                                                                                                                         ggATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGCACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGCGCCaa                                         >  2:211832/1‑300 (MQ=255)                                                                                                                                                                                                                                                               aTGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGCGCCAatgctga                                   >  2:66644/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      tgtTTATCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCAGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTCACGGTAGTTCGTTCGCCCCGGCGGANTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTCCGCGCCAATGCTGANGTTGGt                            >  2:575753/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            tCCCGGCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGCGCCAATGCTGATGCTGGTTATCTa                      >  1:2017/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 gCTGTGGGCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGCGCCAATGCTGATGCTGGTTATCTATTCGt                 >  1:363591/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gggCGAGTTTGTGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGCGCCAATGCTGATGCTGGTTATCTATTCGTTTAAc            <  1:641735/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gggggtgATCTCGGAGCTGCTCGGTGGCCAGGAAAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCCATAACCGCGACTGGACGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACGCCAGCACAAAAGTGTGGGAGAACACGGCTGAGTAATTTACTGGTAGTTCGTTCGCCCTGGCGGATCGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGTGCCAATGCTGATGCTGGTTATCTATTCGTTTAACAGCTCg      >  2:24957/5‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gTTTATGATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGAATGGCCGGTAGTATCGGCGGTAGCGATCGTCGTGTTGCTGCTGCTAATCGTGCCGATCATGTGGTTCCACAAACAACAGCAAAAAAGTGTGGGAGAACACGGATGAATAATTTACCGGTAGTCAGTTCACCCTGGCAGACTGTGATTTTGCTGCCGGGCTTTACCTTTACATACATGCCAATACGAATGCTGGCTACCTGTTCGTTTAACCGCTCg      >  2:25546/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                gtgATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGCGCCAATGCTGATGCTGGTTATCTATTCGTTTAACAGCTCGAAGc  <  2:312986/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                gtgATCTCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGAGCCAATGCTGATGCTGGTTATCTATTCGTTTAACAGCTCGAAGc  <  1:81396/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      ACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGCGCCAATGCTGATGCTGGTTATCTATTCGTTTAACAGCTCGAAGC  >  NC_000913/896615‑897201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑