Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	837,571	A→G	intergenic (+9/+94)	ybiE → / ← ybiJ	protein YbiE/DUF1471 domain‑containing protein YbiJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	837,571	0	A	G	100.0%	222.6 / NA	58	intergenic (+9/+94)	ybiE/ybiJ	protein YbiE/DUF1471 domain‑containing protein YbiJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/26);  total (32/26)

ATTTTGCTACCGGGCGAGTGGGAAGTGAACACGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGCCTGGGATGCCGTTACCGGTTCCGCCGCGAA  >  NC_000913/837275‑837865                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       aTTTTGCTACCGGGCGAGTGGGAAGTGAACACGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCTGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAATAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCgg                                                                                                                                                                                                                                                                                                     <  1:364907/300‑1 (MQ=255) aTTTTGCTACCGGGCGAGTGGGAAGTGAACACGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGTTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCgg                                                                                                                                                                                                                                                                                                     <  1:229640/300‑1 (MQ=255) aTTTTGCTACCGGGCGAGTGGGAAGTGAACACGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCgg                                                                                                                                                                                                                                                                                                     <  1:364788/300‑1 (MQ=255)                         gTGAACACGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGNCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAAAAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGNCGTAGCGTAAACGCCTGCTCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAg                                                                                                                                                                                                                                                                             >  2:630750/1‑300 (MQ=255)                           gAACACGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGAc                                                                                                                                                                                                                                                                           >  1:526543/1‑300 (MQ=255)                                             gCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCCTATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCAGTCGCGTCGCATCAg                                                                                                                                                                                                                                                         >  2:117393/1‑300 (MQ=255)                                                gAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGTTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCACATCAGGCa                                                                                                                                                                                                                                                      <  1:643404/300‑1 (MQ=255)                                                        ggATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGc                                                                                                                                                                                                                                              >  1:522235/1‑300 (MQ=255)                                                             ccACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCAGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCAAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAGCAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGGTTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGAt                                                                                                                                                                                                                                         >  2:188466/1‑300 (MQ=255)                                                                         ggAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAATAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACg                                                                                                                                                                                                                             <  1:583494/300‑1 (MQ=255)                                                                                ggCATGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                                                                                      <  1:547471/300‑1 (MQ=255)                                                                                    ggCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTc                                                                                                                                                                                                                  <  1:684424/300‑1 (MQ=255)                                                                                                            gATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCg                                                                                                                                                                                          >  1:305293/1‑300 (MQ=255)                                                                                                              ttGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCa                                                                                                                                                                                        >  1:258653/1‑300 (MQ=255)                                                                                                                  tATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTAc                                                                                                                                                                                    <  1:327161/300‑1 (MQ=255)                                                                                                                    tGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCg                                                                                                                                                                                  >  2:336159/1‑300 (MQ=255)                                                                                                                       cGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAACAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCNGGACGTAGCGTAAACGCCTGATCCGTNCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCtt                                                                                                                                                                               <  2:458967/300‑1 (MQ=255)                                                                                                                                cGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGTTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTNTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTAttg                                                                                                                                                                      <  1:493203/300‑1 (MQ=255)                                                                                                                                cGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTNTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGGAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTAtgg                                                                                                                                                                      <  1:493197/300‑3 (MQ=255)                                                                                                                                         cTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTtggtg                                                                                                                                                             >  1:123153/1‑300 (MQ=255)                                                                                                                                         cTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAAAATAGGATCAATGAGGGTTCGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGATTAATTTATTGTTGTtggtg                                                                                                                                                             >  2:371929/1‑300 (MQ=255)                                                                                                                                                      gTTAGCCAGCTGAAAAAAAGCCCTTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATATTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGAGTGTGTAGGCCTGATAAGACGCGGTAGCGTCACATCAGACATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTATTGTTGGCGGCGTAAGTGATGc                                                                                                                                                <  2:96761/300‑1 (MQ=255)                                                                                                                                                      gTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGTAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGc                                                                                                                                                <  2:258106/300‑1 (MQ=255)                                                                                                                                                        tAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTg                                                                                                                                              <  1:411376/300‑1 (MQ=255)                                                                                                                                                                gatgtgtataagagacaggTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGNACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACt                                                                                                                                      <  1:477465/282‑1 (MQ=255)                                                                                                                                                                gatgtgtataagagacaggTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACt                                                                                                                                      <  1:118781/282‑1 (MQ=255)                                                                                                                                                                             gTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGcc                                                                                                                         >  2:70267/1‑300 (MQ=255)                                                                                                                                                                                  gTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATcccctctgtctcttatacacatc                                                                                                                    >  2:118230/1‑279 (MQ=255)                                                                                                                                                                                  gTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTNCGGATTTTGTAGGCCTGATAAGACGCNGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACtctgtctcttatacacatc                                                                                                                    >  2:467426/1‑282 (MQ=255)                                                                                                                                                                                                         aaCATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCa                                                                                             >  2:339146/1‑300 (MQ=255)                                                                                                                                                                                                          aCATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAg                                                                                            >  2:293061/1‑300 (MQ=255)                                                                                                                                                                                                          aCATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAg                                                                                            >  1:187740/1‑300 (MQ=255)                                                                                                                                                                                                          aCATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCAGGATGCGGCGTANACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAg                                                                                            >  2:161182/1‑300 (MQ=255)                                                                                                                                                                                                           cATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAgg                                                                                           >  1:588554/1‑300 (MQ=255)                                                                                                                                                                                                                     aggAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTATCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAggg                                                                                 <  1:554237/298‑1 (MQ=255)                                                                                                                                                                                                                         gggTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGa                                                                             >  1:18007/1‑300 (MQ=255)                                                                                                                                                                                                                          ggTTAGAGTATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGACTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCCTCAGGCATCTGTTGCCGGATGTGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTCTTGTTGTTGGTGGCGGAAGTGGTGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGAt                                                                            >  2:216587/1‑300 (MQ=255)                                                                                                                                                                                                                               gagCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGcc                                                                       >  1:104306/1‑300 (MQ=255)                                                                                                                                                                                                                               gagCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTAACGCTTAATTTATTGTTGTTGGTGGTGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGcc                                                                       >  2:98913/1‑300 (MQ=255)                                                                                                                                                                                                                                     atGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGc                                                                 <  2:680274/300‑1 (MQ=255)                                                                                                                                                                                                                                          tCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGaaa                                                            <  2:302319/300‑1 (MQ=255)                                                                                                                                                                                                                                            tGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAAcc                                                          <  1:521195/300‑1 (MQ=255)                                                                                                                                                                                                                                                ggCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGc                                                      <  2:7080/300‑1 (MQ=255)                                                                                                                                                                                                                                                            gggAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCa                                          >  2:213858/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 aTACTTGTGCTGTACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTc                                     <  1:198613/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 aTACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTc                                     <  1:198798/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       gtgCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGcc                               <  2:103884/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       gtgCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGcc                               >  1:43873/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gCTGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGCCTg                             >  1:550193/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           tGGACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGCCTggg                           <  1:423242/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            ggACGTAGCGTAAACGCCTGATCCGTCCAGCNGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATNACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGCCTGGGa                          >  2:496896/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            ggACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGCCTGGGa                          >  1:430886/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            ggACGTAGCGTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCAAGCC‑ATTTTATTCATGTTCTGTGCCTGGGAt                         >  1:300342/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gcgagatgtgtataagagacaggTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGCCTGGGATg                        >  1:384357/23‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gTAAACGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGCCTGGGATGCCGTTAc                 >  1:314505/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          cGCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGNCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGCCTGGGATGCCGTTACCGGtt            >  2:597986/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           gCCTGATCCGTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGCCTGGGATGCCGTTACCGGTTc           <  2:122554/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    gTCCAGCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGCCTGGGATGCCGTTACCGGTTCCGCCGCGaa  >  1:402327/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       ATTTTGCTACCGGGCGAGTGGGAAGTGAACACGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATTACTTATAAATTACCGCAGTACCGCTTAATTTATTGTTGTTGGTGGCGGAAGTGATGCTGTATCCACTGGCACCGGCTGCCGCGGCTTTCTCAGCCAGTTTCGCTTCCAGGGCATCGAGGGTGGATGCGCCATCGGCAGAAACCACGCCGATTTTATTCATGTTCTGTGCCTGGGATGCCGTTACCGGTTCCGCCGCGAA  >  NC_000913/837275‑837865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑